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Citations for Addgene :
151 - 200 of 472 citations for NGS ChIP Seq kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: ... we amplified Ultramers with primers containing sequence homologous to either the STARR-seq luciferase validation vector_mP_empty (Addgene# 99298) or pGL4.23 (Promega ...
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bioRxiv - Microbiology 2020Quote: ... The sgRNA expression construct containing the target sequence 5’-GGTTTGGTTGATTTCTGCAC-3’ was obtained using a PCR-fusion technique and the PCR product was injected at 15 ng/μL together with 50 ng/μL pDD162 (eft-3prom::Cas9, a gift from Bob Goldstein, Addgene plasmid #47549). After injection ...
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bioRxiv - Bioengineering 2020Quote: ... 600 ng of BxbI Integrase Expression Vector (Addgene #51271) and 600 ng of each landing pad donor plasmid were co-transfected ...
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bioRxiv - Synthetic Biology 2021Quote: ... The evoAPOBEC1-BE4max-NG DNA from pBT375 (Addgene# 125616) were cloned between the NotI and PmeI sites of the PB-TRE-Cas9 with NotI restriction enzyme site insertion ...
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bioRxiv - Biophysics 2020Quote: ... 6750 ng of psPAX2 packaging plasmid (Addgene plasmid # 12260), and 750 ng of Spike-18aa truncated (Addgene plasmid # 149541 ...
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bioRxiv - Molecular Biology 2022Quote: ... 400 ng of dCas9-KRAB expression vector (Addgene #60954), 170 ng of lentiGuide puro (Addgene #52963 ...
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bioRxiv - Developmental Biology 2020Quote: ... and 2.5 ng/µl Pmyo-2::mCherry (Addgene #19327). Three injected animals were pooled and incubated for 3 days at 20 °C before adding 250 ng/µl of hygromycin per plate ...
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bioRxiv - Molecular Biology 2021Quote: ... 6750 ng of psPAX2 packaging plasmid (Addgene plasmid # 12260), and either 750 ng of Spike-18aa truncated (Addgene plasmid # 149541) ...
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bioRxiv - Systems Biology 2019Quote: ... and 300 ng of pCAG-mTagBFP2 (Addgene, Plasmid #122373) plasmids ...
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bioRxiv - Genetics 2020Quote: ... and 500 ng of each TALEN-L (Addgene #35431) and TALEN-R (Addgene #35432 ...
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bioRxiv - Systems Biology 2021Quote: ... 70 ng of VP12 humanSpCas9-Hf1 plasmid (Addgene 72247), and 175 ng of donor reporter plasmid ...
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bioRxiv - Genomics 2020Quote: ... 200 ng of CRISPR-KRAB-MeCP2 vector (Addgene 110821) and 50 ng of gRNA gblock (IDT ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and pCFD5-frame_selector_0,1,2 (Addgene 131152; 400 ng/µl each) into nos-Cas9 (attp40) ...
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bioRxiv - Cell Biology 2023Quote: ... 32 ng total/embryo of pCSKA-xwnt8 (Addgene # 16866) plasmid DNA was injected into the 2 dorsal blastomeres of 4-cell stage embryos ...
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bioRxiv - Systems Biology 2023Quote: ... and 500 ng of each TALEN-L (Addgene #35431) and TALEN-R (Addgene #35432 ...
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bioRxiv - Systems Biology 2023Quote: ... with 1000 ng plasmid containing inducible HGF (Addgene #188749) or a constitutive pEF1α-HGF (Addgene #188748 ...
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bioRxiv - Immunology 2023Quote: ... and NG-ABE8e (gift from David Liu, Addgene #138491), and an in vitro transcrition (IVT ...
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bioRxiv - Genomics 2024Quote: ... and transfected with 500 ng pMD-VSVg (Addgene #12259), 1,750 ng psPax2 (Addgene #12260) ...
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bioRxiv - Molecular Biology 2023Quote: ... and 300 ng of pCAG-mTagBFP2 (Addgene plasmid #122373) were combined ...
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bioRxiv - Physiology 2024Quote: ... or 200 ng pDisplay-ATP1.0-IRES-mCherryCAAX (Addgene, #167582) was transfected to HEK293T cells in 24-well plates ...
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bioRxiv - Biochemistry 2024Quote: ... 500 ng of dead SaCas9 plasmid (Addgene no. 138162), 200 ng of SaCas9 sgRNA plasmid ...
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bioRxiv - Immunology 2024Quote: ... 500 ng of pCMV-VSV-G (Addgene plasmid #8454) and 1000 ng of cloned LentiCRISPRv2-Puro plasmid using Lipofectamine (5µl P3000 ...
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bioRxiv - Immunology 2024Quote: ... electroporated with 10 ng pON-mCherry plasmid (Addgene, US) in 200 µl ddH2O using 2-mm cuvettes at 2.5 kV and 200 ν for 4 ms ...
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bioRxiv - Microbiology 2021Quote: ... the TEV FlipGFP plasmid PCDNA3-FlipGFP(TEV cleavage seq) T2A mCherry (Addgene, #124429, a gift from Xiaokun Shu [9]) was used as a template for pairs of PCR reactions including primers designed to generate overlapping products replacing the TEV cleavage site with the indicated cleavage sequence (S9 Table) ...
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bioRxiv - Genomics 2020Quote: ... complete the Illumina sequencing primer sequences and add 15 bp of sequence homologous to the hSTARR-seq (Addgene #99292) plasmid for InFusion cloning ...
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bioRxiv - Microbiology 2022Quote: ... pDSAP-aapp-hGrx1-roGFP2 and pDSAP-trio-DsRed) (~160 ng/µL) and the integrase helper plasmid carrying the Vasa2-ΦC31-Integrase gene (Addgene # 62299, ~200 ng/µL) diluted in 0.5x phosphate buffered saline (0.1 mM NaHPO4 buffer ...
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bioRxiv - Microbiology 2022Quote: ... and 500 ng plentiguide puro expression vector (Addgene cat # 52963), in a final volume of 20 mL with Opti-MEM ...
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bioRxiv - Microbiology 2022Quote: ... The second solution contained 150 ng pCMV-VSVG (Addgene 8454), 400 ng psPAX2 (Addgene 12260) ...
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bioRxiv - Biochemistry 2021Quote: ... 500 ng pMD2.G (Didier Trono lab, Addgene plasmid #12259) and 5 μg of transfer plasmid per plate using polyethylenimine (Merck ...
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bioRxiv - Biophysics 2020Quote: ... and 1250 ng of TMPRSS2 expression plasmids (Addgene plasmid #145843) in the 6-well plate ...
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bioRxiv - Biophysics 2020Quote: ... and 750 ng of Spike-18aa truncated (Addgene plasmid # 149541) were mixed ...
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bioRxiv - Genomics 2021Quote: ... 375 ng of Prime Editor-2 enzyme plasmid (Addgene #132776) and 125 ng of pegRNA plasmid were mixed and prepared with a transfection reagent (Lipofectamine 3000 ...
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bioRxiv - Genetics 2022Quote: ... and pBS-Hsp70-Cas9 (442 ng/μL, Addgene plasmid #45945), which provided Cas9 ...
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bioRxiv - Cancer Biology 2022Quote: ... 680 ng of pCD/NL-BH*DDD (Addgene plasmid # 17531) and either 340 ng of pCEF-VSV-G (Addgene plasmid # 41792 ...
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bioRxiv - Biochemistry 2022Quote: ... and 500 ng of pRK5-HA-Ub-K6 (Addgene, #22900) for 24 h according to the manufacturer’s recommendation ...
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bioRxiv - Developmental Biology 2021Quote: ... and 125 ng expression plasmid (pcDNA3-FOXO::FLAG, Addgene #13507) using manufacturer’s recommendations for lipofectamine 3000 ...
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bioRxiv - Immunology 2021Quote: ... 500 ng pMD2.G VSV-G envelope plasmid (Addgene 12259) and 1 μg shRNA plasmid using Fugene 6 (Promega) ...
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bioRxiv - Molecular Biology 2021Quote: ... NG-ABEmax was a gift from David Liu (Addgene #124163). SpG- and SpRY-ABEmax were a gifts from Benjamin Kleinstiverwas (Addgene plasmids #140002 and #140003) ...
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bioRxiv - Genetics 2023Quote: ... 360 ng pMD2.g VSV-G envelope vector (Addgene #12259), and 1.2 ug of purified plasmid library using 5.8 uL of X-tremeGENE HPTM DNA Transfection Reagent (Millipore Sigma #06366236001) ...
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bioRxiv - Genomics 2021Quote: ... cells constitutively expressing dCas9KRAB were transduced with a library of gRNAs cloned into the CROP-seq-opti vector (Addgene #106280) in order to capture gRNA information on the 10X platform ...
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bioRxiv - Synthetic Biology 2021Quote: ... S2060 bacteria were transformed with a luciferase reporter containing a quadruplet codon at residue 357 (for example, https://benchling.com/s/seq-7CsWcP8Ez4JNjNM9W23N, Addgene #134787) and an inducible qtRNA expression plasmid (for example ...
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bioRxiv - Systems Biology 2021Quote: ... WT HFFs were lentivirally transduced with barcoded Perturb-seq vectors to encode the experimental condition (pBA571, Addgene #85968; Table S3), followed by puromycin selection ...
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bioRxiv - Bioengineering 2023Quote: ... or a GTP/ARCA compatible T7 promoter (Promoter seq: TAATACGACTCACTATAGGAT. Sequences from VEEV were derived from T7-VEE-GFP, a gift from Steven Dowdy (Addgene plasmid #58977 ...
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bioRxiv - Microbiology 2021Quote: ... The gRNA library was synthesized as an oligonucleotide pool (Integrated DNA Technologies) and cloned into CROP-seq-opti (a gift from Jay Shendure, Addgene # 10628095) as described96 ...
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bioRxiv - Cancer Biology 2020Quote: ... A library of 256 CRISPR sgRNA targeting 58 genes was cloned into the CROP-Seq-Guide-Puro expression vector (Addgene #86708) and packaged into lentivirus ...
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bioRxiv - Genomics 2021Quote: ... for 4 h was isolated by formaldehyde-assisted isolation of regulatory elements (FAIRE, Giresi et al., 2007) and subsequently cloned into the STARR-seq screening vector (Addgene #71509) following the protocol described in Arnold et al. ...
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bioRxiv - Genomics 2022Quote: sgRNA-CRISPR library targeting 550 chromatin regulators (Suppl Table 1) was ordered from IDT Technologies and cloned using Gibson assembly in CRISP-seq backbone (Addgene #85707). The Gibson assembly product was electroporated in Endura ElectroCompetent cells following the manufacturer’s protocol (Endura #60242-2 ...
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bioRxiv - Genomics 2023Quote: ... CROP-seq-opti-Puro-T2A-GFP was assembled by adding a T2A-GFP downstream of Puromycin resistant protein coding sequence on the CROP-seq-opti plasmid (Addgene #106280). Flanking MluI and CsiI digestion sites were added to the GFP Gblock (IDT ...
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bioRxiv - Genomics 2023Quote: Perturb-seq GBC library (Adamson et al. 2016, Dixit et al. 2016) was a gift from Jonathan Weissman (Addgene ID #85968). The library contains a random 18-nt guide barcode (GBC ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were transfected with either 500 ng of Super TOPFlash (Addgene) or Super FOPFlash (Addgene ...