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101 - 150 of 2434 citations for trans 2 2 4 Bromophenyl 2 oxoethyl cyclohexane 1 carboxylic acid since 2020

• Identification of a global gene expression signature associated with the genetic risk of catastrophic fracture in iPSC-derived osteoblasts from Thoroughbred horses

  Esther Palomino Lago, et al., bioRxiv - Genetics 2024

  Quote: ... pMXs.hSox2 (SRY-Box Transcription Factor 2, RRID:Addgene_17218), pMXs.hKlf4 (Kruppel Like Factor 4RRID:Addgene_17219) ...
• Zika virus remodels and hijacks IGF2BP2 ribonucleoprotein complex to promote viral replication organelle biogenesis

  Clément Mazeaud, et al., bioRxiv - Microbiology 2024

  Quote: ... we used pcDNA3-GFP-IMP2-2 (Addgene plasmid # 42175 ...
• Coupling proximity biotinylation with genomic targeting to characterize locus-specific changes in chromatin environments

  Pata-Eting Kougnassoukou Tchara, et al., bioRxiv - Biochemistry 2024

  Quote: ... 3 µg of psPAX.2 (Addgene, #12260), and 3 µg of sgRNA plasmid into HEK293T cells seeded in 6-well plates at 70% confluence using jetPRIME transfection reagent according to the manufacturer’s protocol ...
• Cav3.1 is a leucine sensor in POMC neurons mediating appetite suppression and weight loss

  Anthony H. Tsang, et al., bioRxiv - Neuroscience 2024

  Quote: ... 2.) piRES-puro vector (Addgene Cat# 25728) with EcoRI and NotI sites for site-directed mutagenesis experiments.
• Humanized antibody potently neutralizes all SARS-CoV-2 variants by a novel mechanism

  Sai Luo, et al., bioRxiv - Immunology 2022

  Quote: ... The Vκ1-33/CBE replacement of Vκ3-2 was mediated by homologous recombination using a PGKneolox2DTA.2 (Addgene #13449) construct and two guide RNAs that target the mouse Vκ3-2 segment ...
• The ventral CA2 region of the hippocampus and its differential contributions to social memory and social aggression

  Lara M. Boyle, et al., bioRxiv - Neuroscience 2024

  Quote: ... cohort 1) or AAV9.EF1a.dflox.hChR2(H134R).mCherry.WPRE.HGH (1×10^12 pp/mL, Addgene, cohort 2).
• Ensemble cryo-electron microscopy reveals conformational states of the nsp13 helicase in the SARS-CoV-2 helicase replication-transcription complex

  James Chen, et al., bioRxiv - Biophysics 2021

  Quote: ... a pRSFDuet-1 plasmid containing His6-SUMO SARS-CoV-2 nsp12 (Addgene #159107) was transformed into E ...
• Ensemble cryo-electron microscopy reveals conformational states of the nsp13 helicase in the SARS-CoV-2 helicase replication-transcription complex

  James Chen, et al., bioRxiv - Biophysics 2021

  Quote: ... the pCDFDuet-1 plasmid containing His6 SARS-CoV-2 nsp7/8 (Addgene #159092) was transformed into E ...
• Structural basis for substrate selection by the SARS-CoV-2 replicase

  Brandon F. Malone, et al., bioRxiv - Biophysics 2022

  Quote: ... the pCDFDuet-1 plasmid containing His6 SARS-CoV-2 nsp7/8 (Addgene #159092) was transformed into E ...
• Top-down control of hippocampal signal-to-noise by prefrontal long-range inhibition

  Ruchi Malik, et al., bioRxiv - Neuroscience 2021

  Quote: ... 500–550 nl of AAV9-Syn-jGCaMP7f-WPRE virus (diluted 1:2; Addgene) was injected in dorsal CA1 to express synapsin-driven calcium sensor jGCaMP7f (injection coordinates ...
• mtDNA breaks compromise mitochondrial membrane ultrastructure and trigger an integrated stress response

  Yi Fu, et al., bioRxiv - Cell Biology 2022

  Quote: ... 1–2 (lenti-TRE3G-ApaLI(*)-Hygro) were cloned into LT3REVIR (Addgene Plasmid #111176) by inserting mito-ApaLI(* ...
• Vagus nerve stimulation recruits the central cholinergic system to enhance perceptual learning

  Kathleen A. Martin, et al., bioRxiv - Neuroscience 2023

  Quote: ... 0.50 µL of AAV5.FLEX.ArchT.tdTomato (diluted 1:2 with dPBS; Addgene number: 28305) was injected in basal forebrain of ChAT-Cre animals using the coordinates described above ...
• Interareal synaptic inputs underlying whisking-related activity in the primary somatosensory barrel cortex

  Masahiro Kawatani, et al., bioRxiv - Neuroscience 2023

  Quote: ... diluted to 1/2) or AAV-Ef1a-mCherry (#114470-AAV9, obtained from Addgene; titer ...
• Pharmacological inhibition of CLK2 activates YAP by promoting alternative splicing of AMOTL2

  Maya L. Bulos, et al., bioRxiv - Cell Biology 2023

  Quote: ... YAP-targeting shRNA vectors 1 and 2 refer to pLKO1-shYAP1 (Addgene, #27368) and pLKO1-shYAP2 (Addgene ...
• Integration of hunger and hormonal state gates infant-directed aggression

  Mingran Cao, et al., bioRxiv - Neuroscience 2024

  Quote: AAV2/1-syn-GCaMP7s (Addgene 104487, 100–200 nl, 2 × 1013 GC / ml) was unilaterally injected into the MPOA of C57BL/6J mice using a Nanoject II or Nanoject III injector (Drummond Scientific ...
• Trafficking of the glutamate transporter is impaired in LRRK2-related Parkinson’s disease

  Ludovica Iovino, et al., bioRxiv - Neuroscience 2022

  Quote: The pCMV-hEAAT2 plasmid encoding the human excitatory amino acid transporter type 2 (EAAT2) was a gift from Susan Amara (Addgene plasmid # 32814 ...
• Infection and chronic disease activate a brain-muscle signaling axis that regulates muscle performance

  Shuo Yang, et al., bioRxiv - Microbiology 2022

  Quote: pCMV-GFP-SARS-CoV-ORF3a was generated by recombining the SARS-CoV-2-ORF3a coding sequence (pDONR207 SARS-CoV-2 ORF3aA, #141271, Addgene) into pDEST-CMV-N-EGFP (#122842 ...
• Infection and chronic disease activate a brain-muscle signaling axis that regulates muscle performance

  Shuo Yang, et al., bioRxiv - Microbiology 2022

  Quote: UAS-SARS-CoV-2-ORF3a transgenic flies were generated by PCR-mediated subcloning of the SARS-CoV-2-ORF3a coding sequence (pDONR207-SARS-CoV-2 ORF3a, #141271, Addgene) into pUASt-Attb (EcoRI/XbaI) ...
• SARS-CoV-2 and its ORF3a, E and M viroporins activate inflammasome in human macrophages and induce of IL-1α in pulmonary epithelial and endothelial cells

  Magdalena Ambrożek-Latecka, et al., bioRxiv - Immunology 2023

  Quote: ... pDONR207 SARS-CoV-2 M and pDONR207 SARS-CoV-2 ORF3a (all plasmids were gifts from Fritz Roth via Addgene, # 141273 ...
• Novel mechanism of inflammatory activation by Ebola virus matrix protein linked to the ebolavirus virulence

  Satoko Yamaoka, et al., bioRxiv - Microbiology 2024

  Quote: The following open reading frames were cloned into an expression vector under the control of the phosphoglycerate kinase promoter: IKK-2 and its mutant IKK-2 K44M (Addgene plasmids #11103 and #11104 ...
• Differential Kinetics of SARS-CoV-2 Proteases Revealed by a Dual-Color, BRET-based Protease Biosensor, DuProSense

  Asma Fatima, et al., bioRxiv - Biophysics 2024

  Quote: HEK293T cells were co-transfected with mSca-containing DuProSense biosensor plasmid DNA and pLVX-EF1alpha-SARS-CoV-2-nsp5-2×Strep-IRES-Puro (Mpro) (a gift from Nevan Krogan; Addgene #141370 ...
• Differential Kinetics of SARS-CoV-2 Proteases Revealed by a Dual-Color, BRET-based Protease Biosensor, DuProSense

  Asma Fatima, et al., bioRxiv - Biophysics 2024

  Quote: ... along with either pLVX-EF1alpha-SARS-CoV-2-nsp5-2×Strep-IRES-Puro (Mpro) (a gift from Nevan Krogan; Addgene #141370 ...
• Differential Kinetics of SARS-CoV-2 Proteases Revealed by a Dual-Color, BRET-based Protease Biosensor, DuProSense

  Asma Fatima, et al., bioRxiv - Biophysics 2024

  Quote: ... and pmNG-Mpro-NLuc-PLpro-mScarlet either alone or co-transfected with either pLVX-EF1alpha-SARS-CoV-2-nsp5-2×Strep-IRES-Puro (Mpro WT) (a gift from Nevan Krogan; Addgene #141370 ...
• Differential Kinetics of SARS-CoV-2 Proteases Revealed by a Dual-Color, BRET-based Protease Biosensor, DuProSense

  Asma Fatima, et al., bioRxiv - Biophysics 2024

  Quote: ... HEK293T cells were co-transfected with DuProSense biosensor containing Mpro cleavage sites and pLVX-EF1alpha-SARS-CoV-2-nsp5-2×Strep-IRES-Puro (Mpro WT) (a gift from Nevan Krogan; Addgene #141370 ...
• Differential Kinetics of SARS-CoV-2 Proteases Revealed by a Dual-Color, BRET-based Protease Biosensor, DuProSense

  Asma Fatima, et al., bioRxiv - Biophysics 2024

  Quote: ... HEK293T cells were co-transfected with mSca-containing DuProSense biosensor plasmid along with either pLVX-EF1alpha-SARS-CoV-2-nsp5-2×Strep-IRES-Puro (Mpro WT) (a gift from Nevan Krogan; Addgene plasmid # 141370 ...
• Differential Kinetics of SARS-CoV-2 Proteases Revealed by a Dual-Color, BRET-based Protease Biosensor, DuProSense

  Asma Fatima, et al., bioRxiv - Biophysics 2024

  Quote: ... HEK293T cells were co-transfected with mSca-containing DuProSense biosensor plasmid along with pLVX-EF1alpha-SARS-CoV-2-nsp5-2×Strep-IRES-Puro (Mpro WT) (a gift from Nevan Krogan; Addgene #141370 ...
• Differential Kinetics of SARS-CoV-2 Proteases Revealed by a Dual-Color, BRET-based Protease Biosensor, DuProSense

  Asma Fatima, et al., bioRxiv - Biophysics 2024

  Quote: HEK293T cells were co-transfected with mSca-containing DuProSense biosensor plasmid DNA and either pLVX-EF1alpha-SARS-CoV-2-nsp5-2×Strep-IRES-Puro (Mpro) (a gift from Nevan Krogan; Addgene #141370 ...
• Differential Kinetics of SARS-CoV-2 Proteases Revealed by a Dual-Color, BRET-based Protease Biosensor, DuProSense

  Asma Fatima, et al., bioRxiv - Biophysics 2024

  Quote: ... equal amounts of both pLVX-EF1alpha-SARS-CoV-2-nsp5-2×Strep-IRES-Puro (Mpro) (a gift from Nevan Krogan; Addgene #141370 ...
• Intermitochondrial signaling regulates the uniform distribution of stationary mitochondria in axons

  Nozomu Matsumoto, et al., bioRxiv - Neuroscience 2020

  Quote: ... 2 μg of tdTomato-Mito-7 (Addgene #58115) (36 ...
• CAR-NK Cells Effectively Target the D614 and G614 SARS-CoV-2-infected Cells

  Minh Ma, et al., bioRxiv - Immunology 2021

  Quote: pcDNA3.1-SARS-CoV-2 Spike (Addgene plasmid #145302) was used to clone SARS-CoV-2 S gene into the SFG backbone using the In-Fusion Cloning kit (Takara Bio) ...
• DMT1 bridges endosomes and mitochondria to modulate mitochondrial iron translocation

  Jonathan Barra, et al., bioRxiv - Cell Biology 2022

  Quote: ... 2 µg of psPAX2 (Addgene, #12260, MA, USA) and 2 µg of lentiCRISPRv2 sgRNA DMT1 ...
• Caenorhabditis elegans junctophilin has tissue-specific functions and regulates neurotransmission with extended-synaptotagmin

  Christopher A. Piggott, et al., bioRxiv - Genetics 2020

  Quote: ... and pCFJ90 Pmyo-2-mCherry (Addgene plasmid 19327) and pCFJ104 Pmyo-3-mCherry (Addgene plasmid 19328)(Frøkjær-Jensen et al. ...
• Limited Dishevelled/Axin oligomerization determines efficiency of Wnt/β-catenin signal transduction

  Wei Kan, et al., bioRxiv - Biochemistry 2020

  Quote: ... and combined with pX330A-2-PITCh (Addgene #63670) by golden gate cloning ...
• Conditional knockout of Shank3 in the ventral CA1 by quantitative in vivo genome-editing impairs social memory

  Myung Chung, et al., bioRxiv - Neuroscience 2024

  Quote: ... 85 nl of AAV1-hSyn:Cre (Addgene, 2 × 1013) was injected into vCA1 and 200 nl of a 1:1 cocktail of AAV9-pMeCP2:DIO-Cas9 (2 × 1012 GC/ml ...
• Conditional knockout of Shank3 in the ventral CA1 by quantitative in vivo genome-editing impairs social memory

  Myung Chung, et al., bioRxiv - Neuroscience 2024

  Quote: ... and pAAV2/2 (for AAV2; Addgene plasmid # 104963), pAAV2/5 (for AAV5 ...
• Loss of NAT10 disrupts enhancer organization via p300 mislocalization and suppresses transcription of genes necessary for metastasis progression

  Ruhul Amin, et al., bioRxiv - Cancer Biology 2024

  Quote: ... PITCh sgRNA from pX330S-2-PITCh (Addgene #63670) vector was then inserted into the pX330A-1x2-cNAT10 vector using Golden Gate Assembly (New England Biolab ...
• Precision and efficacy of RNA-guided DNA integration in high-expressing muscle loci

  Made Harumi Padmaswari, et al., bioRxiv - Bioengineering 2024

  Quote: ... and exon 2-8 was cloned from Addgene #182141 ...
• Intracellular replication of Pseudomonas aeruginosa in epithelial cells requires suppression of the caspase-4 inflammasome

  Abby R Kroken, et al., bioRxiv - Microbiology 2023

  Quote: ... and pMD.2 (Didier Trono, Addgene plasmid # 12259), combined with either Cas9 expression vector plenti-Cas9-blast (Feng Zhang ...
• A large-scale sORF screen identifies putative microproteins and provides insights into their interaction partners, localisation and function

  Dörte Schlesinger, et al., bioRxiv - Molecular Biology 2023

  Quote: ... PITCh sgRNA from pX330S-2-PITCh (Addgene #63670) was cut-out via Eco31I (Thermo Fisher Scientific ...
• In vivo intra-uterine delivery of TAT-fused Cre recombinase and CRISPR/Cas9 editing unveil histopathology of Pten/p53-deficient endometrial cancers

  Raúl Navaridas, et al., bioRxiv - Pathology 2023

  Quote: ... 2 µg of pCA-mTmG plasmid (#26123, Addgene) was added to diluted TAT-Cre and incubated at 37°C for 1 hour ...
• Differences in syncytia formation by SARS-CoV-2 variants modify host chromatin accessibility and cellular senescence via TP53

  Jonathan D. Lee, et al., bioRxiv - Microbiology 2023

  Quote: ... and pDONR223 SARS-CoV-2 spike (Addgene #149329) were subcloned into pLVpuro-CMV-N-3xFLAG (Addgene #123223 ...
• The FW2.2/CNR protein regulates cell-to-cell communication in tomato by modulating callose deposition at plasmodesmata

  Arthur Beauchet, et al., bioRxiv - Plant Biology 2023

  Quote: ... the AtCas9 (2×35S::AtCAS9-OCST; Addgene #112079) and the linker pICH41780 (Addgene #48019 ...
• Early steps of multi-receptor viral interactions dissected by high-density, multi-color single molecule mapping in living cells

  Nicolas Mateos, et al., bioRxiv - Biophysics 2023

  Quote: The plasmid pr8ΔEnv.2 was obtained from Addgene, Plasmid #12263) ...
• 3-nitrotyrosine shortens axons of a non-dopaminergic neuron by inhibiting mitochondrial motility

  Masahiro Hirai, et al., bioRxiv - Neuroscience 2024

  Quote: ... 2 μg of tdTomato-Mito-7 (Addgene_#58115) (Ai et al. ...
• Reverse engineering of the pattern recognition receptor FLS2 reveals key design principles of broader recognition spectra against evading flg22 epitopes

  Songyuan Zhang, et al., bioRxiv - Plant Biology 2024

  Quote: ... into a level 2 binary vector (Addgene #54346). LRR12-19VvFLS2 fragments carrying computationally predicted polymorphic residue sets (‘comp.Max’ ...
• Reconstitution of BNIP3/NIX-mediated autophagy reveals two pathways and hierarchical flexibility of the initiation machinery

  Elias Adriaenssens, et al., bioRxiv - Cell Biology 2024

  Quote: ... BCL2L13 Y213A/I216A/W276A/I279A (ΔLIR1+2) (RRID:Addgene_223752), BCL2L13 I224A/L227A/W276A/I279A (ΔLIR1+3 ...
• Advanced Peptide Nanoparticles Enable Robust and Efficient delivery of gene editors across cell types

  Oskar Gustafsson, et al., bioRxiv - Bioengineering 2024

  Quote: ... and Prime editor 2 (herein called PE2) (Addgene plasmids ...
• Pancreatic endocrine cells are transduced by adeno-associated virus serotypes 2 and 9 but not 6

  Vishal Ahuja, et al., bioRxiv - Bioengineering 2024

  Quote: ... or pAAV 2/9n for AAV9 (Addgene #112865). Calcium phosphate-based transfection was carried out26 ...
• Soluble CTLA-4 raises the threshold for T-cell activation and modulates anti-tumour immunity

  Paul T. Kennedy, et al., bioRxiv - Cancer Biology 2023

  Quote: ... 4×105 HEK 293T cells were transfected with pCDH-EF1-sCTLA-4 expression plasmid (1.5 µg) and psPax2 (2 µg) and pMD2.G (1.5 µg) packaging plasmids (Addgene), using Viafect™ (Promega ...
• Cell Surface SARS-CoV-2 Nucleocapsid Protein Modulates Innate and Adaptive Immunity

  Alberto Domingo López-Muñoz, et al., bioRxiv - Microbiology 2021

  Quote: ... 2 x 105 cells were seeded on 12-well plates and transiently transfected with 2 μg of plasmids encoding SARS-CoV-2 N (Addgene # 141391) or eGFP (Addgene # 141395 ...