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Citations for Addgene :
101 - 150 of 1751 citations for Vascular Endothelial Cell Growth Factor VEGF Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... Jurkat cells containing pLenti-PE2-BSD (Addgene, #161514) were treated with 10 µg/ml blasticidin for selection of blasticidin and incubated for 72 hours ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then transfected with plasmids (9021,60360,139059 - Addgene) using the Lipofectamine 3000 DNA Transfection Reagent for 48 h ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were transiently transfected with PX459 (Addgene; #48139) and selected with puromycin for 2 days ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were transduced with a pRDA_256 (Addgene #158581) co-expressing an sgRNA and BE3.9max with a puromycin resistance cassette ...
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bioRxiv - Microbiology 2023Quote: ... cells were transfected with pLew100cre-del-tetO (Addgene) [66] and selected with 40 µg/mL ganciclovir ...
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bioRxiv - Cancer Biology 2023Quote: ... cell membrane marker (pLenti-mCherry-CAAX, Addgene #129285), E-cadherin reporter (pHAGE-E-cadherin-RFP ...
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bioRxiv - Cancer Biology 2023Quote: ... or GFP positive cells (when using Addgene #195124) were single cell sorted onto 96-well plates ...
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bioRxiv - Cell Biology 2022Quote: ... cells were infected with pRDA_186 plasmid (Addgene 133458), a gift from John Doench (Broad Institute) ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were transiently transfected with PX459 (Addgene; #48139) and selected with puromycin for 2 days ...
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bioRxiv - Microbiology 2023Quote: ... PEL cell lines expressing lentiCas9-Blast (Addgene, 52962) were previously generated and validated by STR profiling7 ...
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bioRxiv - Cell Biology 2023Quote: ... Cells transfected with EGFP-Actin (Addgene plasmid #31502) (52 ...
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bioRxiv - Cell Biology 2023Quote: PC12 cells were transfected with NPY-pHTomato (Addgene) and stably selected ...
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bioRxiv - Cell Biology 2023Quote: ... cells were transfected with GCaMP7s (Addgene Plasmid #104463)(14) ...
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bioRxiv - Systems Biology 2024Quote: ... BJ1 cells were transduced with Cas9 (Addgene #96924) and selected using 10 µg/mL blasticidin for 7 days ...
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bioRxiv - Physiology 2023Quote: ... HEK293T cells were incubated with pMD2G (#12259, Addgene), psPAX2 (#12260 ...
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bioRxiv - Cancer Biology 2023Quote: ... HPAF-II cells stably expressing FUCas9Cherry (Addgene#70182) were transduced with MSMO1 sg RNA or FAXDC2 sgRNA along with pMD2.G (Addgene #12259 ...
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bioRxiv - Systems Biology 2023Quote: ... Cell (2023) (also deposited in Addgene MORF Collection).
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bioRxiv - Biochemistry 2024Quote: ... coli Δlac cells (Addgene, Didovyk et al., 2017) transformed with plasmids encoding β-gal (wild-type or mutant ...
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bioRxiv - Genomics 2023Quote: ... LNCaP cells stably expressing dCas9-KRAB (Addgene #89567) were seeded in a 6-well plate at a density of 200K cells per well ...
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bioRxiv - Genomics 2023Quote: ... cells were transfected with pMD2.G (Addgene #12259), psPAX2 (Addgene #12260) ...
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bioRxiv - Cell Biology 2024Quote: ... cells were transfected with pMD2.G (Addgene #12259), psPAX2 (Addgene #12260) ...
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bioRxiv - Biochemistry 2024Quote: ... HT22 cells were transfected with psPAX2 (Addgene # 12260), and pMD2.G (Addgene # 12259 ...
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bioRxiv - Cancer Biology 2020Quote: Generation of batch MTR knockout cells in each cell line was achieved using the lentiviral CRISPR–Cas9 vector system LentiCRISPR v2 (Addgene #52961). Briefly ...
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bioRxiv - Biochemistry 2020Quote: HEK293T CRISPR-Cas9 cells were generated by transfecting wild-type 293T cells with pX330-U6-Chimeric_BB-CBh-hSpCas9 vector (Addgene, Plasmid# 42230) carrying single-guide RNA (sgRNA ...
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bioRxiv - Microbiology 2020Quote: RAW 264.7 cells stably expressing FL-Cas9 were generated by transducing RAW 264.7 cells with lentivirus containing LentiCas9-Blast (Addgene plasmid #52962)(Sanjana et al. ...
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bioRxiv - Cancer Biology 2022Quote: ... 22Rv1 STEAP1 ko and PC3 STEAP1 ko cells were generated by transient transfection of 22Rv1 cells with a pool of PX458 (Addgene, #48138) plasmids each expressing one of four different sgRNA targeting sequences predicted from the Broad Institute Genetic Perturbation Platform sgRNA Designer63 ...
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bioRxiv - Genomics 2020Quote: In order to create stable dCas9-KRAB expressing cell line LNCaP cells were infected with lenti-KRAB-dCas9-blast (Addgene, 89567) and selected with 6 µg/ml blasticidin for two weeks ...
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bioRxiv - Cell Biology 2021Quote: ... WT and Lig4-/- abl pre-B cells and MCF10A human mammary epithelial cells used in this study all contain pCW-Cas9 (Addgene# 50661), which has a FLAG-tagged Cas9 cDNA under the control of a doxycycline-inducible promoter ...
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bioRxiv - Microbiology 2021Quote: ... Derivatives of HEK-293T cells expressing ACE2 were generated by transducing HEK-293T (ATCC, CRL-3216) cells with ACE2 (Addgene, #145839). Cells were used as single cell clone was derived by limiting dilution from the bulk populations ...
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bioRxiv - Cancer Biology 2022Quote: ... These constructs were introduced into cells generated by lentiviral transduction of MCF10A TP53-/- cells with lenti-BLAST-dCas9-KRAB (Addgene, #89567) followed by selection with 10 μg/mL blasticidin.
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bioRxiv - Cell Biology 2022Quote: An OgtfloxedCre-ERT2+/KI Rosa26-LSL-YFP+/KI mESC cell line stably expressing Cas9 was generated by lentiviral transduction of the parental cells with lentiCas9-Blast (Addgene #52962). CRISPR sgRNA library lentiGuide-Puro-Brie (Addgene ...
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bioRxiv - Cell Biology 2023Quote: ... LS174T ERN1-/- cells and LS174T ERN2-/- cells were constructed by transfection with pSpCas9(BB)-2A-GFP (Feng Zhang lab, Addgene #48138) containing sequences for guides targeting exon 8 (WGE ID’s 1150303698 and 1150303744 for ERN1 ...
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bioRxiv - Genetics 2023Quote: ... sgRNAs were cloned into a novel construct that had previously been developed for the pooled single-cell RNA sequence profiling of primary cells (CROPseq-Guide- pEFS-SpCas9-p2a-puro; Addgene: #99248).20 The lentivirus was then packaged by transfecting HEK 293FT cells with pCMV delta 8.91 ...
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bioRxiv - Genetics 2023Quote: We plated 550,000 HEK293T cells on 6-well plates and 24 hours later we transfected the cells with 900 ng psPAX2 packaging vector (Addgene #12260), 360 ng pMD2.g VSV-G envelope vector (Addgene #12259) ...
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bioRxiv - Microbiology 2024Quote: HeLa-TetR-Cas9 clonal cells used in the genome-wide screen and polyclonal Huh7 cells transduced with 311-Cas9 (Plasmid Addgene #96924) and selected using 10 µg/mL blasticidin for 7 days were used for secondary screening ...
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bioRxiv - Cancer Biology 2021Quote: ... Flp-In 3T3 p53-/- cell line was generated by Crispr/Cas9 by co-transfecting Flp-In 3T3 cells with px330 p53 plasmid (Addgene Plasmid #59910) expressing Cas9 and sgRNA targeting mouse p53 and pmaxGFP plasmid (Lonza) ...
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bioRxiv - Cancer Biology 2020Quote: ... To generate inducible CRISPR-Cas9 GFPT2 KO cell lines, parental cells (H460, H157, Calu-1) were first infected by pCW-Cas9 plasmid (Addgene plasmid #50661), sorted by puromycin selection ...
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bioRxiv - Cell Biology 2021Quote: Stock of lentiviral particles were obtained by transfection of HEK293T cells (2×106 cells) with 2 μg of lentivector plasmid lentiCRISPRv2 (a gift from Feng Zhang, Addgene plasmid, 52961) expressing single guide RNA targeting an exon within ATG5 gene ...
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bioRxiv - Cancer Biology 2021Quote: ... For generation of KO cell line pools PC-9 and HCC827 cells were transduced with pKLV2-EF1a-Cas9Bsd-W (Addgene ID:68343)[71] to stably express Cas9 ...
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bioRxiv - Biochemistry 2022Quote: ... For transient HER2 overexpression in HEK293 cells, the cells were transfected with a plasmid encoding human HER2 wildtype (Li et al., 2004)(Addgene plasmid #16257) the day before measurement with removal of the transfection reagent after 6 hours ...
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bioRxiv - Cell Biology 2022Quote: ... The S1R-Apex and GFP-Apex plasmids were co-transfected into either HeLa cells (ATCC; CCL-2) or HEK293T cells (ATCC; CRL-3216) along with pXAT2 (Addgene plasmid 80494) (30) ...
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bioRxiv - Molecular Biology 2020Quote: DR-GFP-HCT116 and DR-GFP-U2OS reporter cells were generated by electroporating HCT116 and U2OS cells with Sac1/Kpn1 linearized pHPRT-DRGFP (43) (Addgene, plasmid #26476) DNA ...
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bioRxiv - Cell Biology 2022Quote: ... we first generated a doxycycline-inducible Cas9-expressing THP-1 cell line (iCas9-expressing cells) by transducing THP-1 cells with lentiviruses carrying the Lenti-iCas9-neo plasmid (a gift from Qin Yan; Addgene plasmid #85400). Multiple guide RNAs targeting a gene were cloned into the Lenti-multi-Guide plasmid (a gift from Qin Yan ...
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bioRxiv - Immunology 2019Quote: ... expressing 4T1 tumor cells were generated by transducing cells with lentiviral vector carrying GFP gene (phage ubc nls ha pcp gfp plasmid, Addgene Plasmid #64539).
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bioRxiv - Cell Biology 2021Quote: ... CRISPR-edited NRF2-KO HaCaT cells were produced by transfecting HaCaT cells with pLentiCRISPR-v2 (a gift from Dr Feng Zhang, Addgene plasmid #52961) containing a guide RNA directed against the exon 2 of the NFE2L2 locus (which encodes NRF2 ...
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bioRxiv - Molecular Biology 2022Quote: ... 5 × 106 cells of each cell line were electroporated with a mixture of 10 μg of plasmid pEGFP-D50 lamin A (Addgene plasmid #17653) that had been linearized with EagI and 1 μg of pBABE-puro (Addgene plasmid #1764 ...
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bioRxiv - Molecular Biology 2023Quote: ... Human TMPRSS2 expression in VeroE2T and in VeroE6 cells (VeroE6-TMPRSS2) was achieved by infecting the cells with a 2nd generation lentiviral vector pLEX307-TMPRSS2-blast (Addgene plasmid #158458) as a transfer vector ...
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bioRxiv - Cell Biology 2023Quote: ... Primary MEFs used for the long term cell migration and JLY experiments were infected with viral media from HEK cells transfected with 3x-mScarlet-FTractin (Addgene Plasmid #112960)(53) ...
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bioRxiv - Cell Biology 2023Quote: ... MCF-7 or HeLa single cell colonies were selected from cells transduced with the following sequences targeting human genes cloned into lentiCRISPR v2 (Addgene no. 52961):
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bioRxiv - Cancer Biology 2021Quote: ... CRISPR generated cells were transduced with lentiCRISPRv2 (Addgene # 98290) cloned with specific guides targeting tRNAIleUAU loci (Guide 1 ...