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Citations for Addgene :
101 - 150 of 269 citations for Anti Epidermis type lipoxygenase 12 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... wild-type embryos were injected with a vsx1:Gal4 BAC and UAS:CoChR2-tdTomato plasmid (Addgene Catalog #: 124233) at 20 ng/µL and 25 ng/µL ...
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bioRxiv - Genomics 2022Quote: ... Wild-type (J1) mESCs were transduced with Cas9-blast virus (generated from pLentiCas9-Blast, Addgene ID: 52962) and selected with 10μg/ml blasticidin (InvivoGen ...
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bioRxiv - Cell Biology 2024Quote: Lentiviral-TOP/FOP-dGFP-reporters (wild-type consensus plasmid TOP: Addgene plasmid #14715; http://n2t.net/addgene:14715; RRID:Addgene_14715; inverted consensus plasmid FOP: Addgene plasmid # 14885 ...
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bioRxiv - Microbiology 2023Quote: ... The wild-type (Flag-SIRT1) and mutant (Flag-SIRT1 H363Y) SIRT-1 plasmids were purchased from Addgene and transfected into cells using Lipofectamine 2000 ...
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bioRxiv - Developmental Biology 2023Quote: ... and RYBP wild-type and mutant cDNAs using Gibson assembly into pdCas9-DNMT3A-2A-PuroR (Addgene# 71667)72 ...
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bioRxiv - Immunology 2024Quote: ... DLD-1 wild type cells were transduced with pLenti-CMV-MCS-RFP-SV-puro (Addgene plasmid #109377) viral particles (DLD-RFP ...
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bioRxiv - Cancer Biology 2024Quote: Introduction of N361A by site-directed mutagenesis on the pBABE puro EGFR wild-type (EGFR WT, RRID:Addgene_11011) and pBABE puro EGFR L858R (RRID:Addgene_11012 ...
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bioRxiv - Cancer Biology 2021Quote: ... wild type or mutant FSCN1 (S39E and 149-151A mutants) cDNAs were subcloned into pLenti.CMV.blasticidin vector (Addgene, 17486) (Campeau et al. ...
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bioRxiv - Cell Biology 2020Quote: ... of L-type Ca2+ channels was a gift from Diane Lipscombe (Addgene plasmid # 26572; http://n2t.net/addgene:26572; RRID:Addgene_26572). The mApple-myosin X was subcloned by the Protein Cloning and Expression Core facility of the MBI.
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bioRxiv - Neuroscience 2022Quote: The pCMV-hEAAT2 plasmid encoding the human excitatory amino acid transporter type 2 (EAAT2) was a gift from Susan Amara (Addgene plasmid # 32814; http://n2t.net/addgene:32814; RRID:Addgene_32814). The cDNA was subcloned into pcDNA3 between KpnI and XbaI restriction sites and under the T7 promoter for expression in Xenopus laevis oocytes ...
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bioRxiv - Cancer Biology 2020Quote: ... The full-length wild-type cDNA of human BRCA2 was subcloned from pcDNA3 236HSC WT (Addgene plasmid # 16246) into the piggyBac vector ...
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bioRxiv - Neuroscience 2020Quote: ... we injected rAAV.mDLX.GCaMP6f24 (gift of Jordane Dimidschstein and Gordon Fishell) into wild type mice or injected AAV1.Syn.Flex.GCaMP6f (UPenn Vector Core or Addgene) into Gad2-Ires-Cre C57BL/6J mice (Jackson Laboratory) ...
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bioRxiv - Biochemistry 2020Quote: ... cDNA encoding wild-type human ubiquitin containing an N-terminal HA-tag was expressed from pRK5-HA (Addgene). Full-length EGFP fused N-terminally to a nuclear localization signal (NLS ...
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bioRxiv - Cancer Biology 2022Quote: ... human codon-optimized Streptococcus pyogenes wild-type Cas9 (Cas9-2A-GFP) was obtained from Addgene (Cat. No. 44719). Two chimeric guide RNA expression cassettes containing two of the following sgRNAs (sgRNA1 ...
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bioRxiv - Biochemistry 2022Quote: ... The plasmid expressing FLAG-tagged wild-type human HDAC1 was a gift from Eric Verdin (Addgene Plasmid # 13820). HCT116 cells were transfected in 10 cm plates with 8 μg plasmid and 40 μl PEI ...
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bioRxiv - Neuroscience 2022Quote: ... The wild type aequorin obtained by introducing two reverse mutations into the plasmid [pcDNA3.1+/mit-2mutAEQ] (Addgene #45539) (de la Fuente et al. ...
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bioRxiv - Synthetic Biology 2021Quote: ... mTiam1(64-437)-tgRFP-SspB wild type were a gift from Brian Kuhlman (Addgene plasmid # 60411; http://n2t.net/addgene:60411; RRID:Addgene_60411 ...
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bioRxiv - Microbiology 2023Quote: ... The parental strains Mtb Erdman wild-type and espA-tn were electroporated with the plasmid pKM461 (Addgene, #108320) expressing tetracycline-inducible Che9c phage RecT annealase and Bxb1 phage integrase (80) ...
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bioRxiv - Microbiology 2023Quote: Sequences of all 16 plasmids used for the generation of wild type SA11 strain were obtained from Addgene (https://www.addgene.org/Takeshi_Kobayashi/)5 ...
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bioRxiv - Molecular Biology 2022Quote: ... Synthetic cell type specific promoters were obtained from the following Addgene plasmids: 161_pAAV-ProD5-CatCh-GFP-WPRE (Addgene plasmid # 125981 ...
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bioRxiv - Cancer Biology 2022Quote: ... pCDNA3-V5-PTPN14-wild type was a gift from Jianmin Zhang (Addgene plasmid # 61003; http://n2t.net/addgene:61003 ; RRID:Addgene_61003). siRNAs (pool ...
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bioRxiv - Cell Biology 2024Quote: Lentiviral-TOP/FOP-dGFP-reporters (wild-type consensus plasmid TOP: Addgene plasmid #14715; http://n2t.net/addgene:14715; RRID:Addgene_14715 ...
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bioRxiv - Microbiology 2024Quote: ... Wild-type Myc-MEKK3 (in pcDNA3.1 vector) was generated from a kinase-dead mutant (K391A, Addgene plasmid # 44157)(55 ...
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bioRxiv - Biochemistry 2022Quote: ... The wild-type 14-3-3 ζ gene was cloned into NcoI/XhoI digested pBAD plasmid (Addgene #85482) with a TEV cleavable C-terminal His6 purification tag ...
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bioRxiv - Neuroscience 2024Quote: ... We used the following viral constructs and injection volumes per site in the different types of experiments: AAV.9.Syn.Flex.GCaMP6f.WPRE.SV40 (calcium recordings 140 nl; Addgene, no. 100833); AAV.1.hSyn.dio.EGFP (anterograde labelling ...
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bioRxiv - Neuroscience 2021Quote: ... 200 nL of AAVrg-hSyn-GFP (Addgene 50465-AAVrg; titer 7×10^12 vg/mL) was injected unilaterally at a rate of 2nL/sec ...
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bioRxiv - Neuroscience 2021Quote: ... AAV9 empty vector as control) and AAV1.Tdtomato (5.0E+12 viral genomes /mL; Addgene, 59462) were added to 1 mL culture medium.
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bioRxiv - Neuroscience 2024Quote: ... cohort 1) or AAV9.EF1a.dflox.hChR2(H134R).mCherry.WPRE.HGH (1×10^12 pp/mL, Addgene, cohort 2).
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bioRxiv - Neuroscience 2024Quote: ... DV - 3.8) with 200 nL AAV9.EF1a.dflox.hChR2(H134R).mCherry.WPRE.HGH (1×10^12 pp/mL, Addgene). For subjects in cohort 1 (n=2) ...
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bioRxiv - Cell Biology 2020Quote: ... The plasmid containing the α1C subunit (CaV1.2) of L-type Ca2+ channels was a gift from Diane Lipscombe (Addgene plasmid # 26572 ...
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bioRxiv - Microbiology 2021Quote: ... Plasmids for the expression of wild type spCas9 alone or in combination with gRNA were pcDNA3.3-hCas9 (Addgene #41815) and pX330-T2A-BFP (Addgene # 64216) ...
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bioRxiv - Cancer Biology 2021Quote: ... pcDNA3-based plasmids encoding FLAG-tagged wild type and SATA (S939A/T1462A)-mutant TSC2(51) were obtained from Addgene. The TSC2-5A (S939A ...
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bioRxiv - Neuroscience 2022Quote: ... The AU1-tagged wild-type and two mutant (S2215Y and R2505P) mTOR constructs were gifts from Fuyuhiko Tamanoi (Addgene) 59 ...
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bioRxiv - Cancer Biology 2022Quote: Polyclonal SK-N-DZ cells constitutively expressing the CRISPR activation machinery were engineered by transducing wild-type cells with lentiviral particles carrying a dCas9-VP64 (lenti dCas9VP64_Blast, Addgene plasmid #61425 was a gift from Feng Zhang ...
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bioRxiv - Cell Biology 2021Quote: ... GFP-hRab5B.dn3 (#1008) plasmid encoding GFP-labeled wild-type version of human Rab5B isoform was from Addgene (Cat# 61802). GFP-hRab5B(S34N ...
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bioRxiv - Synthetic Biology 2021Quote: ... Venus-iLID-CAAX (from KRas4B) and pLL7.0: mTiam1(64-437)-tgRFP-SspB wild type were a gift from Brian Kuhlman (Addgene plasmid # 60411 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... a plasmid containing cloned wild type AlkB protein (pET24a-AlkB deltaN11 [plasmid #73622]) was obtained from Addgene (http://www.addgene.org/), and the AlkB protein was expressed and purified at the CSU Biochemistry and Molecular Biology Protein Expression and Purification Facility.
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bioRxiv - Neuroscience 2023Quote: Wild-type and Flailer primary neuronal cultures were infected at 3 DIV with AAV coding for GCaMP6s (Addgene #51086) and FL1 or control AAVs ...
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bioRxiv - Neuroscience 2023Quote: Wild type Drosophila and human spectrins were cloned into a histidine tagged vector (2BC-T cloning vector, Addgene # 31070) using ligation independent cloning to obtain C-terminally tagged proteins ...
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bioRxiv - Cell Biology 2024Quote: ... enables GFP and APEX to be targeted to cilia in many cell types via the N-terminal 203 residues of NPHP3 and was a gift from Maxence Nachury (RRID:Addgene_73186) (Mick et al ...
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bioRxiv - Cell Biology 2023Quote: ... and subcloned into a pGEX-4T1 vector with an N-terminal MBP-tag followed by a TEV cleavage site before wild-type NAP1 (RRID:Addgene_208871), NAP1 delta-NDP52 (S37K/A44E ...
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bioRxiv - Neuroscience 2022Quote: ... N2a cells were co-transfected with the wild-type or FeRIC channels and GCaMP6 (GCaMP6 medium, Addgene cat.40754) or YFP-H148Q ...
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bioRxiv - Neuroscience 2024Quote: Plasmids containing degenerated protein-coding sequences of wild type and mutated D290V hnRNP A2 isoforms were purchased by Addgene. hnRNP B1 sequence were amplified by PCR from hnRNP A2 plasmid ...
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bioRxiv - Cancer Biology 2024Quote: ... harboring the full-length cDNA of human wild type Androgen receptor (AR) (Addgene plasmid # 89078; http://n2t.net/addgene:89078; RRID: Addgene_89078) was procured from Addgene [17] ...
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bioRxiv - Neuroscience 2021Quote: ... 200 nL total of AAVrg-hSyn-GFP (Addgene 50465-AAVrg; titer 7×10^12 vg/mL) was unilaterally injected at a rate or 2 nL/sec into the mPFC (100 nL in IL ...
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bioRxiv - Neuroscience 2023Quote: ... UNC Vector Core) or rAAV5-hSyn-DIO-hM3D(Gq)-mCherry (4.2*10^12 gc/ml; Addgene) in the Locus Coeruleus (LC ...
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bioRxiv - Cell Biology 2024Quote: COS7 cells were seeded over 12 mm cover glasses and co-transfected with the pMitoTimer (Addgene #52659 gently donated by Dr ...
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bioRxiv - Neuroscience 2022Quote: The pCMV-hEAAT2 plasmid encoding the human excitatory amino acid transporter type 2 (EAAT2) was a gift from Susan Amara (Addgene plasmid # 32814 ...
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bioRxiv - Developmental Biology 2020Quote: ... To generate CRISPR Cas9 knockout mESC lines we transfected wild-type (for Srpk1 and Srpk2) or Rlim-/y (for Zfp42) mESCs with pX335 and pKN7 vectors (Addgene) containing gRNA sequences targeting ...
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bioRxiv - Biochemistry 2020Quote: Recombinant wild-type Streptococcus pyogenes Cas9 REC3 domain (506-712) possessing an amino-terminal His10-MBP tag (Addgene, no. 101205) followed by a TEV protease site was expressed in Escherichia coli strain BL21(DE3 ...