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Citations for Addgene :
51 - 100 of 269 citations for Anti Epidermis type lipoxygenase 12 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... wild type mice were injected with AAV5-GFAP-cyto-GCaMP6 (Addgene #52925-AAV5) and AAV1-Syn-NES-jRGECO1a-WPRE (Addgene #100854-AAV1 ...
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bioRxiv - Biochemistry 2024Quote: ... as has the wild-type FLAG Pol η vector (pJRM160) (19) (Addgene # 221897). Vectors expressing FLAG Pol η point mutations or deletions ...
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bioRxiv - Cancer Biology 2024Quote: ... harboring the full-length cDNA of human wild type Androgen receptor (AR) (Addgene plasmid # 89078 ...
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bioRxiv - Cancer Biology 2022Quote: ... lentivirus was produced from the Brunello sgRNA library (12) (Addgene 73178) in 293FT cells (Invitrogen ...
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bioRxiv - Neuroscience 2022Quote: ... AAV2-retro-AAV-CAG-tdTomato (Addgene, 7×10^12 gc/ml), Cholera toxin subunit B CF-640 (Biotium ...
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bioRxiv - Biochemistry 2020Quote: ... wild-type and mutant 3xFLAG-CDK12 sequences were subcloned into lentiCas9-Blast (Addgene 52962) by replacing the Cas9 ORF with 3xFLAG-CDK12 ...
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bioRxiv - Molecular Biology 2023Quote: ... wild-type MEFs were transduced with lentiviral particles containing the plasmids lentiMPHv2 (Addgene #89308) and lentiSAMv2 (Addgene #75112) ...
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bioRxiv - Neuroscience 2024Quote: ... wild type mice were injected with AAV5-GFAP-hM3D(Gq)-mCherry (Addgene #50478-AAV5) into motor cortex (MC ...
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bioRxiv - Immunology 2024Quote: ... The wild-type SARS-CoV-2 spike plasmid (HDM-SARS2-spike-delta21, Addgene #155130) was cloned with the additional D614G substitution ...
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bioRxiv - Bioengineering 2024Quote: ... The wild-type SARS-CoV-2 spike plasmid (HDM-SARS2-spike-delta21, Addgene #155130) was cloned with the additional D614G substitution ...
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bioRxiv - Cancer Biology 2024Quote: ... wild-type DAXX cDNA was cloned into the pInducer20 expression vector (Addgene Plasmid #44012), lentivirus was generated using the psPAX and pMD2.G packaging vectors (Addgene Plasmid #12260 and #12259 ...
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bioRxiv - Biochemistry 2022Quote: ... mCherry was PCR amplified from mCherry-Alpha-5-Integrin-12 (Addgene #54970) using forward primer 54970RMmCherryaddNHis_F2 ...
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bioRxiv - Synthetic Biology 2020Quote: ... 12 μg of pCMV delta R8.2 (gift from Didier Trono, Addgene #12263) and 9 μg of lentiviral vector were diluted in 1.2 ml OptiMEM ...
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bioRxiv - Cell Biology 2021Quote: mApple-Alpha-5-Integrin-12 was a gift from Michael Davidson (Addgene plasmid # 54864 ...
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bioRxiv - Neuroscience 2023Quote: ... AAV1_hSyn1-SIO-stGtACR2-FusionRed (Addgene 105677, 2.1e+12 after 10 fold dilution) was used for optogenetic inactivation ...
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bioRxiv - Cell Biology 2024Quote: ... and 12 μg of pMD2.G VSV-G envelope plasmid (Addgene 12259) with 135 μL of Lipofectamine in 6.75 mL of OptiMEM media (Thermo Fisher 51985091) ...
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bioRxiv - Microbiology 2024Quote: ... coli K-12 MG1655 and LC-E7543 (derivative of MG1655, Addgene #115925) were used for the CRISPRi screen ...
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bioRxiv - Molecular Biology 2020Quote: ... His-tagged wild-type and D135S AlkB plasmids were obtained from Addgene (#79050 and #79051) and proteins were purified by a Ni-NTA column followed by cation exchange ...
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bioRxiv - Cell Biology 2020Quote: ... Wild-type β-PheRS cDNAs were cloned into the pET LIC (2A-T) plasmid (Addgene). Then ...
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bioRxiv - Molecular Biology 2023Quote: ... The type I-B Anabaena variabilis ATCC 29413 CAST was sub-cloned from Addgene (#168137) [16] ...
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bioRxiv - Cell Biology 2022Quote: Full-length ERK3 wild type (WT) pDONR-223 construct purified from Human Kinase Library (Addgene) was used as a template to generate ERK3 K49A K50A kinase dead (KD ...
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bioRxiv - Cell Biology 2022Quote: pHAGE-PIK3CA encoding PIK3CA wild-type (wt; Addgene plasmid #116771; http://n2t.net/addgene:116771; RRID:Addgene_116771) and pHAGE-PIK3CA-H1047R encoding PIK3CA with oncogenic point mutation on p.H1047R (Addgene plasmid #116500 ...
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bioRxiv - Developmental Biology 2024Quote: The plasmid encoding wild-type Yap was obtained from Addgene (pcDNA Flag Yap1, Addgene #18881). Plasmids encoding Yap2YF and Yap3YF were constructed from pcDNA Flag-Yap1 Y357F (Addgene #18882 ...
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bioRxiv - Biochemistry 2024Quote: ... the wild-type human VCPIP1 gene was purchased from Addgene (plasmid #22592 from Wade Harper) (28) ...
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bioRxiv - Biochemistry 2024Quote: DNA plasmids for wild-type PETase (PETase, I. sakaiensis, accession number: A0A0K8P6T7, Addgene number: #112202), hyperactive mutant (Addgene number ...
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bioRxiv - Cell Biology 2024Quote: ... were transfected with 1000 ng of wild-type full-length LRRK2 (pcDNA5-LRRK2, RRID: Addgene_229019) and 500 ng GFP-Rab8A (Addgene ...
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bioRxiv - Genomics 2022Quote: ... Co-transfect 12 μg lentivector with packaging plasmids 9 μg psPAX2 (Addgene, #12260) and 3 μg pMD2.G (Addgene ...
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bioRxiv - Neuroscience 2021Quote: ... AAV5-hSyn-DIO-mCherry (#50459, Addgene, 4.8 x 10^12 GC/mL titer) and AAV5-Ef1a-DIO-hChR2(C128S/D156A)-EYFP (4.9×10^12 titer ...
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bioRxiv - Cancer Biology 2024Quote: ... C-terminally FLAG-tagged ASXL1 p.G646Wfs*12 and p.Y591X were obtained from Addgene. The MSCV-T2A-Puromycin vectors encoding 3xFLAG-tagged full-length ASXL1 and truncated ASXL1 were generated by PCR amplification followed by sub-cloning using Gibson assembly.
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bioRxiv - Cancer Biology 2020Quote: ... wild-type HeLa and MCF10A cells were infected with the dCas9 lentiviral construct (Addgene 61425-LV) and selected with 3μg/ml blasticidin for 5 days ...
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bioRxiv - Biochemistry 2021Quote: URA7 and URA8 wild type genes with ribosomal binding site were cloned into pet28b-6His (Addgene, Massachusetts ...
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bioRxiv - Cell Biology 2023Quote: ... Wild-type and Fam134s KO MEFs were infected with pCW57-CMV-ssRFP-GFP-KDEL (Addgene #128257) in order to generate ER-phagy reporter inducible cell lines ...
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bioRxiv - Cancer Biology 2023Quote: ... wild type TP53 cDNA was amplified from p53-GFP plasmid (Cat #11770) (Addgene, Cambridge, Massachusetts, USA) and cloned into pFN21A (Promega Corporation ...
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bioRxiv - Molecular Biology 2024Quote: ... wild-type K562 cells were transduced with the dCas9-BFP-KRAB lentiviral vector (Addgene Cat#85969). Pure polyclonal populations of CRISPRi-expressing cells were then generated by sorting the transduced cells on a BD FACS Aria II instrument for the top 50% of BFP signal ...
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bioRxiv - Cell Biology 2024Quote: ... Mouse wild-type Pcyt2 cDNA (Horizon Discovery, MMM1013-202763346) was cloned into N174-MCS (Addgene, 81061) backbone digested with EcoRI and BamHI (New England Biolabs ...
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bioRxiv - Cancer Biology 2024Quote: ... Wild-type and mutant CD38 sequences were cloned into the lentiviral expression vector pLX307 (Addgene #41392). Lentiviral particles were produced and used to transduce K562 cells ...
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bioRxiv - Biochemistry 2024Quote: The wild-type HA Pol η expression vector (pJRM56) has been described previously (18) (Addgene # 201671), as has the wild-type FLAG Pol η vector (pJRM160 ...
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bioRxiv - Cell Biology 2022Quote: ... and 12 μg of the pLJM1 vector for overexpression (plasmid #19319; Addgene, Teddington, UK) or 12ug of the pLKO vector for shRNAs (plasmid #8453 ...
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bioRxiv - Developmental Biology 2023Quote: ... A mixture of 12 μg of the validated retroviral vectors pSIREN_EGFP-siLuc (Addgene #122287) 21 expressing GFP under the control of PGK promoter and 9 μg of a VSVG plasmid ...
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bioRxiv - Neuroscience 2024Quote: ... or pAAV-hSyn-DIO-mCherry (4.2*10^12 gc/ml, Addgene, United States, addgene.org) were bilaterally injected into the mPFC ...
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bioRxiv - Neuroscience 2024Quote: ... 250 nL of pENN.AAV9.CaMKII.GCaMP6s.WPRE.SV40 (concentration of 1.0E+12 viral genomes/mL, Addgene, 107790) was injected into the right primary motor cortex to specifically target layer 2&3 neurons ...
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bioRxiv - Cell Biology 2024Quote: ... kit using 3 µg of mouse Septin 12-GFP plasmid (pEGFP-C1, Addgene, USA) and 3 µg of mouse Lamin B1 or Lamin B2 or Lamin B3 plasmids conjugated with myc-tag (pCS2-MT ...
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bioRxiv - Microbiology 2024Quote: ... The aTc-inducible CRISPR/FnCas12a plasmid system (PB025, ∼12 kb) from Addgene (PB025 #183092) was engineered to target gadB ...
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bioRxiv - Cancer Biology 2020Quote: ... Human codon-optimized Streptococcus pyogenes wild type Cas9 (Cas9-2A-GFP) was obtained from Addgene (Cambridge, MA). Chimeric guide RNA expression cassettes with different sgRNAs (sgRNA1 ...
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bioRxiv - Cell Biology 2020Quote: The doxycycline-inducible PLK4 plasmid was generated by PCR of the wild-type PLK4 cDNA from Addgene plasmid #41165 by PfuUltra II Fusion HS DNA polymerase (Agilent #600670. ...
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bioRxiv - Bioengineering 2020Quote: ... either of the two cell types was subjected to Lipofectamine transfection with CMV-R-GECO1.2-mCherry (Addgene, Watertown ...
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bioRxiv - Biochemistry 2020Quote: Recombinant wild-type and mutant SpCas9 (1-1,368) possessing a C-terminal decahistidine tag (Addgene, no. 62731) was expressed and purified as described previously (35) ...
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bioRxiv - Synthetic Biology 2021Quote: ... mTiam1(64-437)-tgRFP-SspB wild type were a gift from Brian Kuhlman (Addgene plasmid # 60411; http://n2t.net/addgene:60411; RRID:Addgene_60411, and Addgene plasmid # 60417 ...
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bioRxiv - Microbiology 2020Quote: Plasmids for lentiviral transduction (pLJM1-2xStrep or untagged wild-type ORF68, mutants, and homologs) (Addgene #x-x) were generated by subcloning into the AgeI and EcoRI sites of pLJM1 modified to confer resistance to zeocin (Addgene #19319 ...
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bioRxiv - Molecular Biology 2023Quote: ... The type I-F Vibrio cholerae HE-45 CAST was sub-cloned from Addgene (#130637 and #130633) [14] ...