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Citations for Addgene :
1151 - 1200 of 1446 citations for Onion Yellow Dwarf Virus OYDV PCR Kits since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... The cDNA of interest was amplified by PCR using primers (Table S4) with overhangs that allow directional cloning into EcoRV-linearized pHIVdTomato (#21374, Addgene) using the 2X Gibson Assembly Kit (Cat # E2611S ...
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bioRxiv - Cancer Biology 2024Quote: ... A sequence containing the 2.4-kbp p21 promoter region was PCR amplified from the pGL2-p21 promoter- Luc (Addgene #33021). The mCherry-3X-NLS sequence was PCR amplified from CSII-prEF1a- mCherry-3X-NLS (Addgene #125262) ...
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bioRxiv - Molecular Biology 2024Quote: ... We amplified this sequence by PCR with primers that have 40 bp overlap to the digested ends (BamH1+XhoI) of the target plasmid (pCR Zero, #120275, Addgene) (primers ...
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bioRxiv - Neuroscience 2024Quote: The mtHyPer7 H2O2 sensor targeted to the mitochondrial matrix of astrocytes was PCR amplified from pCS2+MLS-HyPer7 (donated by Dr. Vsevolod Belousov, Addgene plasmid 136470; RRID: Addgene_136470) with primers containing SalI and HindIII restriction sites 5’ and 3’ to the coding sequence ...
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bioRxiv - Developmental Biology 2024Quote: ... and LaG17 were PCR-amplified from pHR-SFFV-LaG17-synNotch-TetRVP64 (a generous gift from Wendell A. Lim, Addgene #79128). Nucleotide fragments of EGFP and hPDGFRB(512-561 ...
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bioRxiv - Cell Biology 2024Quote: ... The target sites were PCR amplified with primers containing gRNA target sequences using the pCBC-DT1T2 vector as a template (Addgene plasmid # 50590 ...
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bioRxiv - Biochemistry 2024Quote: ... DNA sequences for 8xHis-MBP TRPA11089-1119 and 3xFlag-6xHis-WT CaM were PCR amplified and subcloned into a pET28a vector (Addgene) at the NcoI/NotI sites using In-Fusion EcoDry cloning ...
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bioRxiv - Bioengineering 2024Quote: ... Gluc-RMA was amplified by polymerase chain reaction (PCR) from the vector AAV-hSyn1-Gluc-RMA-IRES-EGFP (Addgene, 189629), and its DNA was extracted using a Zymoclean Gel DNA Recovery Kits (ZYMO) ...
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bioRxiv - Cell Biology 2024Quote: ... UbC-HA-NLS-tdMCP-GFP and UbC-GFP were PCR amplified from pHAGE-UBC-HA-NLS-tdMCP-GFP (Addgene #40649) and Lenti-UbiC-GFP11 ...
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bioRxiv - Cell Biology 2024Quote: ... 3423 bp was provided by Colin Johnson) and for GFP were generated by PCR-based cloning into a pDC315 vector (Addgene). For GFP-MEGF10 ...
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bioRxiv - Cell Biology 2023Quote: ... The TRUPATH kit was purchased from Addgene (#1000000163) and was a gift from Bryan Roth ...
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bioRxiv - Synthetic Biology 2023Quote: ... a gift from John Dueber (Addgene kit # 1000000061). The constructs encoding sequential incorporation of serine in place of tyrosine in the FUS IDR was based on previously published sequences66 ...
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bioRxiv - Synthetic Biology 2024Quote: ... a gift from Christopher Voigt (Addgene Kit #1000000137), were utilized in this study as PCR templates and/or intermediate DNA assembly hosts (19).
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bioRxiv - Neuroscience 2020Quote: ... The plasmid kit used for the generation of TALENs was a gift of Daniel Voytas and Adam Bogdanove (Addgene kit #1000000016). Plasmid encoding assembled TALENs was linearized with SacI ...
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bioRxiv - Plant Biology 2020Quote: ... GFP tagged OsHIPP19 and OsHIPP20 were generated by Golden Gate methods (Engler et al. 2008) using MoClo Plant Parts Kit and MoClo Plant Tool Kit (Addgene, UK). OsHIPP19 and OsHIPP20 were cloned into pICH47732 with a GFP tag at the N-terminus ...
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bioRxiv - Biochemistry 2024Quote: Plasmids encoding GPCRs were obtained from various sources (as indicated Key Resources Table) or subcloned into pcDNA3.1 from the PRESTO-Tango GPCR kit (Addgene Kit #1000000068) as described next ...
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bioRxiv - Plant Biology 2024Quote: ... Plasmid construction was performed by modular cloning using the MoClo Tool Kit and the MoClo Plant Parts Kit (Addgene, 28–30). A detailed MoClo protocol and the list of vectors used can be found in 18.
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bioRxiv - Neuroscience 2022Quote: ... we cloned the dgn-1 promoter and AcNPV p10 3’UTR into the vector pPD49.26 (from the Andrew Fire plasmid kit, Addgene Kit #1000000001) to generate plasmid pNTC2 ...
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bioRxiv - Cell Biology 2023Quote: ... were constructed in according to Golden Gate TALEN assembly protocol and using the Golden Gate TALEN and TAL Effector Kit 2.0 (Addgene, Kit #1000000024). CIscript-GoldyTALEN was a gift from Daniel Carlson & Stephen Ekker (Addgene ...
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bioRxiv - Cell Biology 2024Quote: ... The 7x sgRNAs targeting Gramd1a/b were selected using the tool CRISPOR v5.2 [62] and assembled as previously described using the multiplex CRISPR/Cas9 assembly kit [63] (Addgene kit # 1000000055). sgRNAs sequences and oligos used for cloning are provided in Supplemental Table 5 ...
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bioRxiv - Molecular Biology 2020Quote: ... A C-terminal Strep tag on ORF24-NTD was added by inverse PCR to generate p6H-SUMO3-ORF24-NTD-Strep (Addgene #138467).
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bioRxiv - Cell Biology 2020Quote: For derivation of HA-Smo and Smo-HA ESCs mouse Smo was amplified by PCR from the pGEN-mSmo (Addgene, #37673) and introduced in the PB-HA-IRES-Neo vector ...
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bioRxiv - Cell Biology 2020Quote: ... To generate PCP-NLS-2xmCherry, we PCR amplified PCP from phage UbiC NLS HA stdPCP st-dGFP (Horvathova et al., 2017) (Addgene #104099) using primer sequences 5’-ATACTCGAGCGCCAC-CATGGGCCCAAA and 5’-CTCATCGATGGTGGC-GACCGGTGGAC ...
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bioRxiv - Cell Biology 2020Quote: ... To generate MCP-NLS-2xGFP, we PCR amplified 2xEGFP from phage UbiC NLS HA stdPCP stdGFP (Horvathova et al., 2017) (Addgene #104099) using primer sequences 5’-TCTATCGATATGGTGAGCAAGGG-CGAG and 5’-ATAGCGGCCGCTTATTTGTACAAT-TCATCCATACCATGGG ...
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bioRxiv - Cell Biology 2020Quote: ... To generate the modified TREAT reporter constructs, we first PCR amplified humanized Renilla luciferase from the original TREAT plasmid (Horvathova et al., 2017) (Addgene #104096) using primer sequences 5’-ATACTCGAGCGACTCACTATAGGCTAGCCAC and 5’-ATAGGCCGGCCTTACTGCTCGTTCTTCAG-CAC ...
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bioRxiv - Cell Biology 2020Quote: ... We then excised the IRES-H2B-RFP cassette and cloned in place a 5’ copy of YFP lacking a stop codon, which we PCR amplified from IRES-H2B-YFP-DD (Han et al., 2014) (Addgene #96893) using primer sequenc-es 5’-AAGTTATCGATATGGTGAGCAAGGGCGAGG and 5’-GCATGGACGAGCTGTACAAGAGCGGC-CGCATGGT ...
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bioRxiv - Genetics 2021Quote: ... For the pPB_TRE3G::dCas9-5XGCN4_EF1a::TetOn-Hygro, the Streptococcus pyogenes dCas9GCN4 was PCR amplified from the PlatTET-gRNA2 plasmid (Morita et al., 2016) (Addgene #82559), and cloned together with a d2 destabilization domain under control of the TRE3G promoter in a PiggyBac backbone vector also containing the TET-ON3G transactivator and the Hygromycin resistance gene separated by an IRES sequence and under control of the CAG promoter ...
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bioRxiv - Molecular Biology 2021Quote: ... To express TagRFP658 and mCardinal in neurons under CaMKII promoter the corresponding genes were PCR amplified and swapped with the Arch-GFP gene in FCK-Arch-GFP (Addgene #22217) using InFusion cloning ...
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bioRxiv - Neuroscience 2021Quote: ... was PCR amplified from the pBid-UAS-myr plasmid (Akbergenova et al., 2018) and fused with the GCaMP7s cDNA (Addgene # 104463) (Dana et al. ...
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bioRxiv - Neuroscience 2021Quote: ... The donor was made by amplifying homology arms from the genome by PCR and fusing them by Gibson assembly with a cDNA coding for 6xHis-mClover3 (Addgene #74252) (Bajar et al. ...
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bioRxiv - Molecular Biology 2021Quote: We in-house generated a lentiviral construct expressing dCas9-KRAB-MeCP2 by PCR amplification of the dCas9-KRAB-MeCP2 (contains a domain of MeCP2) from pB-CAGGS-dCas9-KRAB-MeCP2 (Addgene 110824), and then insert it to the pLenti-EF1a-dCas9-VP64-2A-Blast backbone (Addgene 61425 ...
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bioRxiv - Cell Biology 2022Quote: CymR was constructed via PCR and Gibson Assembly from the following constructs: pCuo CA Rac1 CMV + cumate operon purchased from Addgene (#84643), human APC open reading frame purchased from Addgene (#16507) ...
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bioRxiv - Cell Biology 2022Quote: ... The mCherry2-NSP2 expressing plasmids were created by cloning a PCR-amplified NSP2 fragment into the mCherry2-C1 (Addgene plasmid 54563) and mCherry2-N1 vectors (Addgene plasmid 54517 ...
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bioRxiv - Molecular Biology 2021Quote: ... GFP ORF was PCR-amplified and cloned between AgeI and EcoRI sited of lentiviral vector with synapsin I promoter (Addgene #20945). During this cloning AgeI site was destroyed and a new AgeI was introduced on a PCR primer downstream of GFP ...
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bioRxiv - Genomics 2020Quote: ... The INTS6 ORF was PCR amplified with 5’ Xho I and 3’ EcoRI restriction site overhangs and the coding sequence for a C-terminal V5 epitope tag was added in frame to the 3’ end of the INTS6 ORF (Key Resources Table. The INTS6 PCR product and MSCVpuro vector (Addgene 68469) were digested with XhoI (NEB R0146 ...
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bioRxiv - Bioengineering 2021Quote: ... The nCas9(D10A) was generated by the PCR method using previously optimized Cas9 as a template (Level 1 hCas9 module, Addgene #49771). Desired sgRNA sequence was PCR amplified using plasmid pICH86966::AtU6p::sgRNA_PDS (Addgene #46966 ...
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bioRxiv - Bioengineering 2021Quote: ... nanobody sequences were PCR amplified with primers containing terminal regions of homology for cloning into a pRset plasmid (Addgene plasmid 3991)(Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... The UAS sites and K10 3’UTR were replaced via PCR with the squash promoter and squash 3’UTR from pBS-Squ-mCherry (Eric Wieschaus56, Addgene 20163). The RhoGEF2 ORF was obtained from the DGRC (SD04476) ...
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bioRxiv - Cell Biology 2021Quote: These constructs were subcloned via PCR from the corresponding squ-mNeongreen-RhoGEF2 full length constructs into Ac5-Stable2-neo (Rose Barrio and James Sutherland60, Addgene 32426).
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bioRxiv - Cell Biology 2021Quote: These constructs were subcloned via PCR from the corresponding squ-mNeongreen-RhoGEF2 full length constructs into pGEX6P1-N-HA (Andrew Jackson and Martin Reijns, Addgene 119756).
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bioRxiv - Neuroscience 2020Quote: ... pAAV-hsyn1-mGFP was generated by inserting PCR-amplified mGFP between restriction sites BamHI and HindIII of pAAV-hSyn1-ChR2-EGFP (Addgene #58881). The plasmid pAAV-Ef1a-DIO-hChR2(E123T/T159C)-EYFP was obtained from Addgene (#35509).
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bioRxiv - Neuroscience 2020Quote: To generate pAAV-hsyn1-mGFP-T2A-Cre we amplified EGFP-T2A-Cre by PCR from pRetroX-GFP-T2A-Cre (Addgene #63704) and inserted this fragment between restriction sites BamHI and HindIII of pAAV-hSyn1-ChR2-EGFP (Addgene #58881) ...
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bioRxiv - Biochemistry 2020Quote: ... BsaI sites were added to ends by PCR with complementary overhangs for golden gate cloning into pATT-Dest (Addgene plasmid #79770) using primers MBP-BsaI-GG-F and MBP-BsaI-GG-R (the sequences for all primers used for cloning can be found in Table S1) ...
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bioRxiv - Cell Biology 2021Quote: ... The expression vector for N-terminally FLAG-tagged mouse SYDE2 was generated by PCR amplification of the coding sequence from pNICE HA-mSYD1B (Addgene #59362) and insertion into pcDNA3-FLAG by Gibson assembly.
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bioRxiv - Cell Biology 2021Quote: ... Mito-mCh-1×FLAG and Mito-mCh-smFLAG were constructed by ligating 1×FLAG synthesized by overlapping PCR and smFLAG amplified from smFLAG-KDM5B-24×MS2 (Addgene # 81084) with previously built Mito-mCh-1×HA cut by BglII and BamHI through Gibson Assembly.
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bioRxiv - Neuroscience 2020Quote: ... Rnd2 and DNRhoA were cloned by PCR using pNeuroD1-Rnd2 (Heng et al., 2008) and pRK5-myc-RhoA-T19N (Addgene 12963) as templates respectively and then inserted into the SfiI/PmeI sites of the CAG-Neurog2-IRES-DsRed retroviral vector (Heinrich et al. ...
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bioRxiv - Molecular Biology 2020Quote: ... Guide RNA resistant ORC2 (ORC2gr) was amplified by PCR and cloned into NheI-digested mAID-mCherry2-NeoR plasmid (mAID-mCherry2-NeoR, Addgene 72830) in order to add mAID degron sequence to the N-terminus ...
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bioRxiv - Plant Biology 2021Quote: ... 3356 bp upstream of the RPT2 start codon were amplified by PCR with primers 13Rb-RPT2F and 13Rb-RPT2R and cloned into the AL13Rb plasmid (Addgene # 161006) (Alamos et al. ...
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bioRxiv - Cancer Biology 2021Quote: ... cDNA encoding RNF43 without stop codon was amplified by the PCR and cloned into the pcDNA3.1 MCS-BirA(R118G)-HA (Addgene plasmid #36047) using HpaI (ER1031 ...
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bioRxiv - Microbiology 2022Quote: ... with a 15 base pair overlap with vector backbone were used in a polymerase chain reaction (PCR) to generate fast Timer protein timer fragment from plasmid pFast-FT-N1 (Addgene 31910). Five tandem repeats of Tax responsive element (TRE ...