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Citations for Addgene :
1151 - 1200 of 1312 citations for En 1948 4 Marker Pcb Extraction Spike 13C12 99% 100 Ng Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: Adult mice were stereotaxically injected at 7-10 weeks of age with 600 nl of 1.5×1013 vg/ml AAV1-CKIIa-stGtACR2-FusionRed (AddGene) or 7×1012 vg/ml AAV1-CKIIa-mCherry control at bilateral ACC (antero-posterior (AP ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... experimental animals received bilateral infusions (0.5 µL/hemisphere) of AAV5-hSyn-DIO-hM3D(Gq)-mCherry (7×1012 vg/mL; Addgene) into the VTA as described in [32] ...
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bioRxiv - Neuroscience 2023Quote: ... a mixture of flexed AAV GFP (pAAV-FLEX-GFP-Virus, titer ≥ 1×10¹³ vg/mL, Addgene 28304-AAV PHPeB) and CamKII-Cre (pENN.AAV.CamKII 0.4.Cre.SV40 ...
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bioRxiv - Neuroscience 2023Quote: ... we injected the Cre-dependent constructs AAV-EF1a-DIO-ChrimsonR-mRuby2-KV2.1-WPRE-SV40 (5×1011 gc/mL; Addgene) and AAV-EF1a-DIO-eNpHR3.0-mCherry-WPRE (5×1012 gc/mL ...
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bioRxiv - Neuroscience 2023Quote: ... followed by insertion of a 10uL Hamilton syringe loaded with 1.5uL of AAV/DJ-hSyn1-DIO-tdTomato-T2A-Syp::eGFP-WPRE (2.5 × 1013 GC/mL from Addgene #51509). Following intravitreal delivery ...
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bioRxiv - Neuroscience 2023Quote: ... AAV5-hSyn-DIO-hM4D(Gi)-mCherry or AAV5-hSyn-DIO-mCherry (all viruses: 4.2*10^12 gc/ml; Addgene) in the LC (AP ...
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bioRxiv - Neuroscience 2023Quote: The virus and transgenes infused were AAV2/9-Ef1α-DIO-eGFP-htauE14 (2.05e13 vg/mL; Virovek CA, USA; the htauE14 plasmid, a kind donation from Karen Ashe, Addgene), or AAV2/9-Ef1α-Flex-eGFP (1.8E13 vg/mL ...
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bioRxiv - Neuroscience 2023Quote: ... 1 μl and 500 nl of AAV9-hSyn-GRAB-rDA1m (2 x 10^13 vg/ml; Addgene, 140556-AAV9) were injected into the dorsal striatum (AP 0.5 mm ...
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bioRxiv - Neuroscience 2023Quote: ... After 2 weeks the same procedure was used to injected 200nL of AAV5-hSyn1-SIO-eOPN3-mScarlet-WPRE (3,4 10^12 gc/mL Addgene) at the same previous locations inside the RSC ...
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bioRxiv - Neuroscience 2023Quote: ... AAVrg-hSyn-fDIO-hM3D(Gq)-mCherry-WPREpA (1.8x1012 gc/ml) was a gift from Ulrik Gether (Addgene plasmid # 154868); AAV8-hSyn-DIO-mCherry (3.6x1012 gc/ml ...
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bioRxiv - Neuroscience 2024Quote: ... and the AAV9 hM3D DREADD (AAV-hSyn-DIO-hM4D(Gq)-mCherry) (titer = 4.5 × 1012 vg/mL; Addgene; 44361-AAV9) was stereotaxically injected into the OV/MEPO of Nos1-cre female and male mice ...
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bioRxiv - Cancer Biology 2023Quote: ... A 10-μl Hamilton syringe was used to infuse 1 μl of AAV1/Syn-GCaMP6f-WPRESV40 (titer 4.65 × 1013 GC per ml, via Addgene) into the parabrachial nucleus (−5.3 mm anteroposterior ...
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bioRxiv - Neuroscience 2024Quote: ... a total volume of 10-20 ul of AAVrg-FLEX-taCasp3-TEVp (titer >1 × 1012 pfu/ml; Addgene 45580) was injected into the medial and left lobes of the livers of AvilCreERT2 mice ...
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bioRxiv - Neuroscience 2024Quote: ... Either 375nl AAV9-CAG-FLEX-tdTomato (wheel running and optogenetic control, Addgene 28306-AAV9, 3.8 x 1013 gc/mL) or AAV9-CAG-Flex-ChR2-tdTomato (optogenetic stimulation ...
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bioRxiv - Neuroscience 2024Quote: ... rAAV5/hSyn Coff/Fon hChR2(H134R)-EYFP (University of North Carolina Vector Core #8476, titer 5.8 x 1012 GC/mL (Fenno et al., 2014)) and rAAV8/nEF-ChRmine-oScarlet (Addgene #137160-AAV8 ...
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bioRxiv - Neuroscience 2024Quote: ... into the DR and bilateral infusions of AAVretro-hSyn-DIO-EGFP (200 nL over 2 min; 1.3 x 1013 GC/mL, Addgene) into the BLA ...
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bioRxiv - Neuroscience 2024Quote: ... we intracranially injected 400 nL of AAV5-hSyn-DIO-EGFP (Addgene 50457-AAV5; titer: 1.3 x 1012 vg/mL) into the right BLA at coordinates AP ...
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bioRxiv - Cancer Biology 2024Quote: ... at a titer of >1x109 vg/ml.121 AAV-mDlx-NLS-mRuby2 was a gift from Viviana Gradinaru (Addgene plasmid #99130 ...
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bioRxiv - Neuroscience 2023Quote: ... 1 µL of AAV9.hsyn.FLEX.iGluSnFR.WPRE.SV40 (a gift from Loren Looger, Addgene plasmid # 98931; http://n2t.net/addgene: 98931; RRID:Addgene_98931, 10 vg/mL in water) solution was injected with a blunt tip (30 gauge ...
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bioRxiv - Neuroscience 2021Quote: ... were infused bilaterally with AAV expressing the inhibitory designer receptor human M4 muscarinic receptor (hM4Di; AAV8-hSyn-hM4Di-mCherry, 4.8 × 1012 or 3.7 × 1012 vg/mL; Addgene; 0.3 μL) at a rate of 0.1 μL/min into the mOFC (AP ...
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bioRxiv - Neuroscience 2021Quote: ... were infused bilaterally with AAV expressing the inhibitory designer receptor hM4Di (AAV8-hSyn-hM4Di-mCherry, 4.8 × 1012 or 3.7 × 1012 vg/mL; Addgene; 0.4 μL) at a rate of 0.1 μL/min into the BLA (AP ...
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bioRxiv - Neuroscience 2022Quote: ... For stimulation of Drd1-Cre+ UBCs we injected AAV5-EF1a-DIO-hChR2(H134R)-eYFP-WPRE-hGH (7.2×1012 vg/ml, Addgene, 20298P). For experiments with optogenetic stimulation of Pkj cells we injected AAV1-PCP2-CHR2-mCherry (7.2×1012 vg/ml ...
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bioRxiv - Neuroscience 2021Quote: ... and the control virus AAV2/5-hGFAP-EGFP-5’miR-30a-shRNA(scramble)-3’miR-30a-WPREs (AAV-shscramble, 2.57 × 1012 vg/mL) were synthesized based on the pAAV-hGFAP-EGFP-WPRE-hGH plasmid (Addgene # 105549) and packaged by Brain VTA (Wuhan ...
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bioRxiv - Neuroscience 2021Quote: ... AAV2/1-hSynapsin-1-jGCaMP8 constructs (pGP-AAV-syn1-jGCaMP8f-WPRE, Addgene plasmid #162376, 4e12 GC/mL titer; pGP-AAV-syn1-jGCaMP8m-WPRE, Addgene plasmid #162375 ...
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bioRxiv - Neuroscience 2019Quote: ... CA) or the excitatory DREADD receptor-encoding virus (AAV5-hSyn-DIO-hM3Dq-mCherry, 6.50E+12 GC/mL, Addgene, Watertown, MA). Viral incubation occurred for at least 8 weeks during the post-surgical recovery ...
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bioRxiv - Neuroscience 2020Quote: ... injections of rAAV2-retro-Cre (produced by Salk Vector Core or Vigene, 2×1012 to 1×1013 viral genomes/ml, produced with capsid from Addgene plasmid #81070 packaging pAAV-EF1a-Cre from Addgene plasmid #55636 ...
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Basolateral amygdala parvalbumin neurons report aversive prediction error to constrain fear learningbioRxiv - Neuroscience 2020Quote: ... AAV5-ef1α-DIO-hChR2(H134R)-eYFP (5.5×1012 GC/ml) (pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA was a gift from Karl Deisseroth (Addgene viral prep # 20298-AAV5 ...
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bioRxiv - Neuroscience 2020Quote: Optogenetic transduction of neurons was achieved using AAV9-CaMKii-hChR2(E123T/T159C)-mCherry (Addgene 35512; 3.3 × 1013 genome copies/ml) obtained from University of Pennsylvania Vector Core or Addgene ...
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bioRxiv - Neuroscience 2021Quote: ... which provided a higher titer (AAV5-hSyn-ChR2-eYFP, UPenn Lot: CS1078 based on Addgene 26973P, titer: 3.828e13 GC/ml). All injections were made in five different locations in a plus-shaped pattern within the chamber and separated by 2 mm horizontally and vertically (see example in Supp ...
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bioRxiv - Neuroscience 2020Quote: ... viral vectors encoding DREADDs for ChI manipulation were infused into the NAcore of ChAT::cre animals: AAV5-hsyn-DIO-HM4D(Gi)-mCherry (inhibitory; titer: 1.2×1013 vg/mL; N=15 [Addgene, #44362]), AAV5-hsyn-DIO-rM3D(Gs)-mCherry (excitatory ...
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bioRxiv - Cancer Biology 2021Quote: ... and after selection in 300 μg/ml G418 cells were tagged with luciferase by transduction with lentiviruses expressing pFU-Luc2-eGFP (Addgene).
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bioRxiv - Microbiology 2022Quote: Chemical-genetic screens were initiated by thawing 2 × 1ml aliquots (1.0 OD600 units/mL) of the Mtb CRISPRi library (RLC12; Addgene 163954) and inoculating each aliquot into 19ml 7H9 supplemented with kanamycin (10 μg/mL ...
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bioRxiv - Neuroscience 2020Quote: ... AAV1.hSyn.GCaMP6f.WPRE.SV40 (120 nl, 2×1013 vg/mL Penn Vector Core/Addgene; diluted 1:2 to 1:10 in saline) was injected either into V1 ...
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bioRxiv - Neuroscience 2021Quote: ... unilaterally with 70 nl viral mixture of AAV pCAG-FLEX-{EGFP}on (Addgene plasmid #51502, titer 1.1E+12 GC/ml,) and AAV-{CAR}off (titer 1E+12 GC/ml)104 at 200 µm depth from the bulb surface ...
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bioRxiv - Neuroscience 2020Quote: ... RFP-GFP-LC3-encoding retrovirus (9.3×10 TU/mL, produced at the Molecular Tools Platform at the CERVO Brain Research Center based on plasmid #21074, Addgene, kindly provided by Dr ...
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bioRxiv - Neuroscience 2020Quote: ... CMV-PercevalHR (1×1010 TU/mL, produced at the University of North Carolina Vector Core Facility based on plasmid # 49083, Addgene, kindly provided by Dr ...
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bioRxiv - Neuroscience 2020Quote: To express oChIEF-mCitrine in pOXR1-Cre mice, a recombinant adeno-associated virus (rAAV, serotype 1, 4.8×1013 VC/ml titer) expressing FLEXed oChIEF-mCitrine (Addgene #50973) was package by Vector Biolabs (Malvern ...
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bioRxiv - Neuroscience 2021Quote: The single-stranded AAV vector ssAAV-9/2-hSyn1-chI-dlox-Igk_(rev)-dlox-WPRE-SV40p(A) (1.2 × 1013 vg/ml) was produced using the plasmid by Viral Vector Facility at University of Zurich (Addgene #121922). For intravitreal viral injections of the AAV ...
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bioRxiv - Neuroscience 2021Quote: ... 1 µL of 4.6 x 1012vg/mL AAV5-hSyn-DIO-hM4D(Gi)-mCherry (UNC Viral Vector Core; (Krashes et al., 2011)) or 7 x 1012vg/mL AAV5-hSyn-DIO-mCherry (Addgene) was injected at 2.1 mm below the surface of the brain (Andrews-Zwilling et al. ...
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bioRxiv - Neuroscience 2022Quote: ... DV: −4.4) we infused 450nL of pAAV5- Ef1a-DIO-(hChR2-E123A)-eYFP (Addgene-ChR2 & eYFP titre: 2.2x10^13 GC/mL)76 ...
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bioRxiv - Neuroscience 2022Quote: ... using an ultra-fine dental drill and inserted a glass pipette backfilled with AAV2-CamkIIa-hChR2(H134R)-EYFP (titer: 3×10¹² viral genomes per ml, 26969-AAV2, Addgene). AC was approached similar to extracellular recordings ...
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bioRxiv - Neuroscience 2022Quote: ... CRF1-cre mice were injected with 500 nL/hemisphere of AAV5-hSyn-DIO-eGFP (50457-AAV5; titer ≥ 7×10¹² vg/mL, Addgene) into the VTA (ML ±0.60 ...
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bioRxiv - Microbiology 2023Quote: ... and lentiviral particles expressing human TMPRSS2 (selection with 1 mg/ml G418). The TMPRSS2 (cat. 145843) and ACE2 (cat. 145839) lentiviral DNA clones (23) were purchased from Addgene and transfected into HEK293T cells together with pSPAX2 (Addgene ...
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bioRxiv - Neuroscience 2022Quote: ... AAV9-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA (300 nL; 3×1011 GC/ml; Serotype:9; Addgene, Massachusetts, USA) was unilaterally injected into the right MePD using a 2-μL Hamilton micro syringe (Esslab ...
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bioRxiv - Neuroscience 2022Quote: ... 800-1000 nL of a AAV5-hSyn-dLight1.2 vector (~1 × 1013 viral genomes/mL, obtained from Addgene; catalog #AAV5-111068) was delivered into the LNAc (antero-posterior ...
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bioRxiv - Neuroscience 2022Quote: ... 6 mice from each line were injected bilaterally with a pAAV9-CAG-Flex.GCaMP6s.WPRE.SV40 virus (Addgene, titre ≥ 1×1013 vg/mL) in the medial NAc shell (D1-cre and D2(A2a)-cre mice ...
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bioRxiv - Neuroscience 2023Quote: ... The following viruses were injected:: AAV5-hSyn-DIO-hM3Dq-mCherry (DREADD-Gq, titer 6 × 1012 cfu/ml, 250ul bilateral, #44361, Addgene), AAV5-hSyn-DIO-mCherry (control ...
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bioRxiv - Neuroscience 2023Quote: ... injections of rAAV2-retro-Cre (produced by Salk Vector Core or Vigene, 2×1012 to 1×1013 viral genomes/ml, produced with capsid from Addgene plasmid #81070 packaging pAAV-EF1a-Cre from Addgene plasmid #55636 ...
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bioRxiv - Neuroscience 2022Quote: ... titer ≥ 7× 1012 vg/mL; gift from Edward Boyden and purchased through UNC Vector Core; now commercially available from Addgene viral preparation #37825-AAV5 ...
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bioRxiv - Neuroscience 2022Quote: ... titer ≥ 7× 1012 vg/mL; gift from Edward Boyden and purchased through UNC Vector Core; now commercially available from Addgene viral preparation #29777-AAV5 ...