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Citations for Addgene :
1051 - 1100 of 1457 citations for En 1948 4 Marker Pcb Extraction Spike 13C12 99% 100 Ng Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: WM266-4 or SK-MEL-2 cells were co-transduced with conditioned media containing lentiviruses bearing Firefly luciferase-7TFP (Addgene #24308, β-catenin reporter) and Renilla luciferase pLenti.PGK.blast-Renilla Luciferase (Addgene #74444 ...
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bioRxiv - Biochemistry 2022Quote: CRISPR-Cas9-mediated gene ablation was carried out using a guide RNA targeting Cmas exon 4 (5’-TGTCGACGAGGCCGTTTCGC-3’) in the plasmid pX330-U6-Chimeric_BB-CBh-hSpCas9 (Addgene plasmid #42230 from Feng Zhang) as used before.11 TSC were cotransfected with GFP-expressing plasmid peGFP-CI (Clontech ...
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bioRxiv - Neuroscience 2024Quote: ... polyA_R, Table 3) and plasmid backbone (Col1E_F, Col1E_R, Table 4) were amplified from pPRISM-Stop-cmlc2-eGFP (Addgene kit #1000000154; Wierson et al., 2020). The PCR-amplified fragments were assembled using NEBuilder HiFi DNA Assembly Cloning Kit (E5520S ...
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bioRxiv - Neuroscience 2023Quote: ... 60-100 nl AAV2-CAG-FLEX-GCaMP6f viruses (Vigene, custom prepared) or AAV1-CAG-FLEX-GCaMP6f viruses (Addgene, 100835-AAV1, 3x dilution) were delivered unilaterally into each of the following targeted brain regions as described previously (Fang et al. ...
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bioRxiv - Neuroscience 2023Quote: ... or left IC (0.9 mm caudal and 1 mm lateral from lambda suture) to inject 100-200 nL of pAAV-Syn-Chronos-GFP virus (Addgene #59170-AAV1). At the end of the surgery ...
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bioRxiv - Neuroscience 2024Quote: ... or juvenile (P28-30) Grpr::eGFP mice were injected bilaterally with 250 nL (neonates) or 100 nL (juveniles) AAV5-hSyn-mCherry (Addgene: 114422-AAV5) diluted 1:200 to label neurons in the NAc MSh ...
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bioRxiv - Neuroscience 2021Quote: ... 200 nL of AAVrg-hSyn-GFP (Addgene 50465-AAVrg; titer 7×10^12 vg/mL) was injected unilaterally at a rate of 2nL/sec ...
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bioRxiv - Neuroscience 2021Quote: ... AAV9 empty vector as control) and AAV1.Tdtomato (5.0E+12 viral genomes /mL; Addgene, 59462) were added to 1 mL culture medium.
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bioRxiv - Neuroscience 2021Quote: ... or AAV5-hSyn-Con/Foff-hChR2(H134R)-EYFP (5.3e12 vg/ml, UNC, Addgene plasmid #55646) into SNr ...
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bioRxiv - Neuroscience 2019Quote: ... All viruses were used at a titer of 1012 GC/mL and acquired from Addgene.
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bioRxiv - Neuroscience 2020Quote: ... AAV5-hsyn-DIO-rM3D(Gs)-mCherry (excitatory; titer: 1.3×1013 vg/mL; N=10 [Addgene, #50485 ...
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bioRxiv - Neuroscience 2022Quote: ... AAV vectors encoding ChR2(H134R)-eYFP (titer = 1 x 1012 vg/ml, Addgene 26973, RRID:Addgene_127090) were stereotaxically injected into and centered at the temporal association cortex (TeA ...
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bioRxiv - Neuroscience 2022Quote: ... AAV vectors encoding ChR2(H134R)-eYFP (titer = 1 x 1012 vg/ml, Addgene 26973, RRID:Addgene_127090) were stereotaxically injected into and centered at the temporal association cortex (TeA ...
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bioRxiv - Neuroscience 2022Quote: pAAV-CAG-tdTomato (titer ≥ 1×10¹³ vg/mL) was a gift from Edward Boyden (Addgene viral prep #59462-AAV9 ...
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bioRxiv - Neuroscience 2022Quote: ... or pAAV-hSyn-DIO-mCherry (Control mCherry reporter; Addgene #50459, titers: 7×1012 vg/ml) into the VTA ...
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bioRxiv - Neuroscience 2022Quote: ... AAV9-EF1a-DIO-hChR2(H134R)-EYFP (Addgene, 1.8*10^13 gc/ml, diluted 1:10), AAV2-hSyn-hChR2(H134R)-EYFP (UNC Vector Core ...
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bioRxiv - Neuroscience 2022Quote: ... AAV encoding AAV5- CBA-FLEX-ArchT-tdTomato (Titer: 1.3 × 1013 GC/mL) (#28305; Addgene, USA) after 10-fold dilution with PBS was used ...
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bioRxiv - Neuroscience 2022Quote: ... AAV vectors containing Cre-inducible GCaMP6f (AAV.9.Syn.Flex.GCaMP6f.WPRE.SV40, Addgene; TIter≥2.1×13 GC/ml) were injected into the right DLS or DMS by stereotaxic surgery ...
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bioRxiv - Molecular Biology 2022Quote: ... a 2 mL transfection mixture containing lentiviral packaging plasmids 7.5 µg pMD2.G (#12259, Addgene), 11.4 µg pMDLg-RRE (#12251 ...
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bioRxiv - Neuroscience 2021Quote: ... pAAV-hSyn-hM4D(Gi)-mCherry (serotype AAV retrograde, viral titer ≥ 7×1012 vg/mL; Addgene), and Clozapine N-oxide (CNO ...
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bioRxiv - Neuroscience 2022Quote: ... AAV2/1-DIO-CAG-GCaMP6f was purchased from Addgene (# 100839, titer: 1.4 × 1013 gc/ml). To chemogenetically activate PVNOT neurons by means of DREADD we employed AAV2/1-DIO-hSYN1-hM3Dq-mCherry (Addgene # 44361 ...
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bioRxiv - Neuroscience 2022Quote: AAVretro-Cre (pENN-AAV/retro-hSyn-Cre-WPRE-hGH, 2.10E+13 vg/mL, Addgene, USA) was injected in three locations (100 nl/each ...
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bioRxiv - Neuroscience 2023Quote: ... AAVrg-EF1a-DIO-FLPo-WPRE-hGHpA (titer 1.6 × 1013 vg/ml, 250nl bilateral, #87306, Addgene), AAV-DJ-hSyn-fDIO-hMD4Gi-mCherry (titer 2 × 1013 vg/ml ...
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bioRxiv - Neuroscience 2023Quote: ... Retro-AAV2 coding for mCherry (pAAV2-hSyn-mCherry, Addgene #114472, 2 x 1013 GC/ml) and green fluorescent protein (pAAV2-hSyn-eGFP ...
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bioRxiv - Microbiology 2022Quote: ... 500 ml LB culture of pCDFDuet-1-6×His-SARS-CoV-2-NSP7/NSP8 (Addgene)-transformed E.coli BL21 DE3 strain was induced by isopropyl β-d-1-thiogalactopyranoside (IPTG ...
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bioRxiv - Neuroscience 2022Quote: ... at titer ≥7x1012 vg/mL or 12μl AAV1 SYN-GCaMP6f-WPRE-SV40 (Addgene #100837-AAV1) at titer ≥1x1013 vg/mL per 24 wells ...
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bioRxiv - Neuroscience 2024Quote: AAV serotype 9 hSyn-FLEx(FRT)-mGFP (7.4 × 1012 gp/mL) (Corresponding plasmid: Addgene #71761)85
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bioRxiv - Neuroscience 2024Quote: ... plus either AAV8-hSyn-DIO-hM4Di-mCherry (2.2 x 1013 GC/mL, Addgene, Watertown, MA) or AAV8-hSyn-DIO-mCherry (Control virus ...
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bioRxiv - Neuroscience 2024Quote: ... and 0.1 µL of AAV2-pCAG-FLEX-eGFP (Addgene #51502; titer: 7×10¹² vg/mL) was injected into the SNr during the same surgery ...
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bioRxiv - Neuroscience 2023Quote: ... and the injections of AAV5-hSyn-NES-his-CaMPARI2-WPRE-SV40 (2.5x10^12gc/ml Addgene 200nl measured using a Nano Injector ...
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bioRxiv - Neuroscience 2024Quote: ... we injected a mix of AAV1.Syn.jGCaMP7f (Addgene 104488; dilution 1:10 ∼ 1x1013 GC/mL) and AAV1.Syn.Flex.tdTomato (Addgene 28306 ...
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bioRxiv - Neuroscience 2024Quote: ... AAV-syn-FLEX-jGCaMP7b-WPRE (FLEX-GCaMP7b, 1.90e+13 gc/mL, 250nl, Addgene #104493-AAV1), ssAAV-retro/2-hSyn1-mCherry_iCre-WPRE-hGHp(A ...
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bioRxiv - Neuroscience 2023Quote: ... into the VP and retro-AAV Cre (0.4uL, titer ∼ 1.03 × 1014 GC/mL, Addgene #51507,) into the ipsilateral VTA at a rate of 0.1uL/min ...
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bioRxiv - Neuroscience 2023Quote: ... the pipette containing AAVPHP.S-CAG-FLEX-tdTomato virus (Addgene 28306-PHP.S, 1.8×1013 vg/mL) targeted at 2 locations in the mandibular branch spaced 250 um apart ...
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bioRxiv - Neuroscience 2023Quote: ... AAV1-Syn-GCaMP6f-WPRE-SV40 (titer: 2.8 × 10^13 GC/mL) was purchased from AddGene and was diluted 1:4 in sterile 1× PBS ...
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bioRxiv - Neuroscience 2023Quote: ... cre-dependent AAV9-hSyn-FLEX-GCaMP6s-WPRE-SV40 (∼2.0 x 1012 vg/ml; Addgene #100845) was injected unilateral into the VTA (Coordinates ...
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bioRxiv - Neuroscience 2023Quote: ... cre-dependent AAV9-hSyn-FLEX-GCaMP6s-WPRE-SV40 (∼2.0 x 1012 vg/ml; Addgene #100845) was bilaterally injected into the ARC (Coordinates ...
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bioRxiv - Neuroscience 2023Quote: ... we injected AAV-EF1a-DIO-ChrimsonR-mRuby2-KV2.1-WPRE-SV40 (5×1011 gc/mL; Addgene) bilaterally into the lateral hypothalamus of the previously characterized and validated MCH-Cre mice42 ...
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bioRxiv - Neuroscience 2023Quote: ... AAV5-Syn-PSAM4-GlyR-IRES-eGFP (2.4 × 10^13 GC/mL) was purchased from AddGene. Mice had the virus injected at stock titer bilaterally into dorsal and ventral hippocampus ...
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bioRxiv - Neuroscience 2023Quote: Unilateral stereotaxic injections of AAV5-CaMKIIα-EGFP (Addgene #50469; virus titer ≥ 3×10¹² vg/mL) and AAV5-hSyn-DIO-hM4D(Gi)-mCherry (Addgene #44362 ...
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bioRxiv - Neuroscience 2023Quote: ... and AAV5-hSyn-DIO-hM4D(Gi)-mCherry (Addgene #44362; virus titer ≥ 7×10¹² vg/mL) viruses were performed on adult anesthetized (isoflurane ...
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bioRxiv - Neuroscience 2024Quote: ... mice were injected in LHA with AAV5-hSyn-DIO-eGFP (1*10^12vc/ml; Addgene) and co-injected with rAAV5-ORXpr1-3TdTomato (1*10^12 gc/ml ...
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bioRxiv - Neuroscience 2024Quote: Mice were injected with 0.7μl of pGP-AAVrgsyn-jGCaMP7f-WPRE virus (1.85× 1013GC/ml; Addgene) in the nucleus accumbens (NAc ...
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bioRxiv - Neuroscience 2024Quote: ... cohort 1) or AAV9.EF1a.dflox.hChR2(H134R).mCherry.WPRE.HGH (1×10^12 pp/mL, Addgene, cohort 2).
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bioRxiv - Neuroscience 2024Quote: ... a 1:1 mixture of AAV8-hSyn-DIO-EGFP (2.3 x 1013 CG/mL, Addgene) and AAV8-Flex-3mts-mScarlet (1.14 x 1013 CG/mL ...
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bioRxiv - Neuroscience 2024Quote: ... 200 nl of lenti pSICO.DIO.shRheb (1.00 X 1013 GC/ml, packaged by Vigene, Addgene #81087) was bilaterally injected into the medial prefrontal cortex [-2.00 mm anterior posterior (AP) ...
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bioRxiv - Neuroscience 2024Quote: ... DV - 3.8) with 200 nL AAV9.EF1a.dflox.hChR2(H134R).mCherry.WPRE.HGH (1×10^12 pp/mL, Addgene). For subjects in cohort 1 (n=2) ...
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bioRxiv - Physiology 2024Quote: ... ChATCre mice received an AAV.Php.eB-flex-tdTomato (titer; 1.9×1013 pfu/ml, Addgene 28306-PHP.eB) injection into the liver ...
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bioRxiv - Immunology 2021Quote: ... and the third exon of NCR3 (conserved in 3 of the 4 longest isoforms of NCR3 with transcript ID ENST00000376073) were designed and cloned into the lentiCRISPR V2 vector (Addgene #52961, sgRNA sequences in Table S1). Lentiviruses were produced in HEK293T cells using standard laboratory protocols ...
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bioRxiv - Bioengineering 2020Quote: ... The eluted fractions were then pooled together and underwent TEV cleavage overnight at 4°C (TEV protease was purified using the plasmid pRK793, #8827 from Addgene, a gift from David Waugh Lab).