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Citations for Addgene :
1101 - 1150 of 2227 citations for 8 phenylamino 5 4 5 sulpho 1 naphthyl azo 1 naphthyl azo naphthalene 1 sulphonic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: ... pLenti CMVTRE3G Puro DEST (w811-1) was a gift from Eric Campeau (Addgene plasmid # 27565). Both plasmids harbor a puromycin- resistant gene as a marker for antibiotic selection ...
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bioRxiv - Biophysics 2022Quote: ... pLenti-CMV-Puro-DEST (w118-1) was a gift from Eric Campeau & Paul Kaufman (Addgene plasmid # 17452 ...
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bioRxiv - Cell Biology 2021Quote: ... the GST-tagged anti-GFP nanobody construct in pGEX-6P-1 vector (Addgene ID # 61838) was transformed to BL21 RIL (DE3 ...
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bioRxiv - Cancer Biology 2020Quote: Two distinct shRNA for each target gene were cloned into pLKO.1 puro (Addgene #8453) according to the corresponding protocol ...
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bioRxiv - Neuroscience 2021Quote: AAV5.CaMKII.hChR2 (H134R)-mCherry (100 μL at titer ≥ 1 × 10 13 vg/mL) from Addgene was injected in 4-week-old rats intracerebrally in the right somatosensory forepaw region ...
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bioRxiv - Biochemistry 2020Quote: ... amplifying the vector from DU67831 and the Nsp3 1-109 with linker sequence from Addgene plasmid 141257 pDONR207 SARS-CoV-2 Nsp3.
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bioRxiv - Cancer Biology 2021Quote: ... they were recombined into the AgeI-EcoRI-digested pLKO.1-TRC cloning vector (Addgene #10878), using T4 DNA ligase (Thermofisher ...
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bioRxiv - Physiology 2022Quote: ... pSF-lenti or pLKO.1 together with the two helper plasmids psPAX2 (Addgene plasmid 12260) and pMD2.G (Addgene plasmid 12259 ...
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bioRxiv - Biochemistry 2019Quote: ... and then cloned into the lentivirus expression plasmid pLenti CMV Puro DEST (w118-1) (Addgene) by Gateway cloning (ThermoFisher ...
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bioRxiv - Developmental Biology 2019Quote: ... cloned in pLKO.1 puro vector and confirmed as per the protocol from addgene (Addgene Plasmid 10878 ...
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bioRxiv - Molecular Biology 2021Quote: ... The pSFFV_mNG2(11)1-10 plasmid was a gift from Bo Huang (Addgene plasmid # 82610) (Feng et al. ...
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bioRxiv - Molecular Biology 2020Quote: ... 1-1.5μl of Forward and Reverse primers and 50ng of lenti-sgRNA plasmid (Addgene; 52963) in a 50μl total volume reaction ...
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bioRxiv - Cancer Biology 2022Quote: ... the reporter plasmid was first built upon the pLKO.1 - TRC cloning vector (Addgene #10878), by substituting the Puromycin resistance gene with the p53mClover2-P2A-mRFP1 or mClover2-P2A-mRFP1 (empty control ...
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bioRxiv - Cell Biology 2022Quote: ... 2011) (pCMV-VSV-G and pLKO.1 puromycin were gifts from R. Weinberg through Addgene), using Lipofectamine 3000 (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: HEK293T cells were plated 1 day before transfection with psPax2 (viral packaging plasmid; Addgene; 12260), pCMV-VSV-G (viral envelope plasmid ...
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bioRxiv - Neuroscience 2022Quote: ... AAV2/1-DIO-CAG-GCaMP6f was purchased from Addgene (# 100839, titer: 1.4 × 1013 gc/ml). To chemogenetically activate PVNOT neurons by means of DREADD we employed AAV2/1-DIO-hSYN1-hM3Dq-mCherry (Addgene # 44361 ...
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bioRxiv - Microbiology 2022Quote: ... 500 ml LB culture of pCDFDuet-1-6×His-SARS-CoV-2-NSP7/NSP8 (Addgene)-transformed E.coli BL21 DE3 strain was induced by isopropyl β-d-1-thiogalactopyranoside (IPTG ...
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bioRxiv - Neuroscience 2022Quote: ... pAAV2/1 (Penn vector core) or rAAV2-retro helper (Addgene, #81070 (Tervo et al., 2016)) ...
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bioRxiv - Molecular Biology 2022Quote: ... that had been linearized with EagI and 1 μg of pBABE-puro (Addgene plasmid #1764) which contains a puromycin resistance gene ...
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bioRxiv - Cell Biology 2022Quote: ... pcDNA3.1-hMcl-1 was a gift from Roger Davis (Addgene plasmid 25375, Cambridge, MA, USA) and is indicated in the text as WT Mcl-1 ...
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bioRxiv - Cancer Biology 2024Quote: ... Efficient nuclease activity was confirmed using a reporter system (Addgene #67979, #67980; Supp. Fig. 1). We synthesized a custom single guide RNA (sgRNA ...
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bioRxiv - Neuroscience 2024Quote: ... we injected a mix of AAV1.Syn.jGCaMP7f (Addgene 104488; dilution 1:10 ∼ 1x1013 GC/mL) and AAV1.Syn.Flex.tdTomato (Addgene 28306 ...
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bioRxiv - Neuroscience 2024Quote: ... 1:20 of final mix) was mixed with AAV5-hSyn-DIO-hM3D(Gq)-mCherry (Addgene 44361 ...
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bioRxiv - Neuroscience 2024Quote: ... at a 1:40 dilution for sparse cell targeting and AAV8-hSyn-dio-GFP (Addgene #50457-AAV8 ...
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bioRxiv - Immunology 2024Quote: Our SAM.1 (PB-UniSAM) parent vector was a gift from Lesley Forrester (Addgene 99866), that itself was a polycistronic derivative of Feng Zhang’s dual vector system (Addgene 61422 ...
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bioRxiv - Biochemistry 2024Quote: ... two all-in-one CRISPR-Cas9 vectors were generated from pX330A-1×2 (58766, Addgene) and pX330S-2-PITCh (63670 ...
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bioRxiv - Developmental Biology 2023Quote: ... ASEC-1 cells were transfected with a Cas9 plasmid carrying puromycin resistance (Addgene pSpCas9(BB)-2A-Puro (PX459 ...
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bioRxiv - Plant Biology 2023Quote: ... These level 0 parts were assembled into the level 1 Loop acceptor (pCk2; Addgene #136696) in a one-step restriction-ligation reactions with a double CaMV35s-ΩTMV promoter/5′ UTR (pICH51288 ...
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bioRxiv - Cancer Biology 2023Quote: ... pLenti CMV Puro LUC (w168-1) was a gift from Eric Campeau & Paul Kaufman (Addgene plasmid #17477 ...
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bioRxiv - Molecular Biology 2023Quote: ... an shRNA targeting human UNK gene was cloned in the pLKO.1 puro plasmid (Addgene_8453) between AgeI and EcoRI sites61 ...
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bioRxiv - Evolutionary Biology 2023Quote: 1) Cas9 vector to target the C terminus of Nelfe gene: PX459 vector (Addgene 62988) was digested using BbsI-HF (NEB ...
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bioRxiv - Cell Biology 2023Quote: ... The open reading freame (ORF) of EGFP was amplified from pEGFP-C2 (#6083-1, Addgene) using upstream and downstream primers located at the BstbI and XbaI cleavage sites ...
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bioRxiv - Cell Biology 2023Quote: The expression vector of talin 1-RFP was a gift from Michael Davidson (Addgene #55139). FL TSM and HP35st TSM were gifts from Carsten Grashoff (Addgene plasmids #101170 and #101251) ...
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bioRxiv - Molecular Biology 2023Quote: ... 1000k pelleted hiPSCs were mixed with 1 μL 10 μM SP-dCas9-VPR (Addgene, 63798), 9 μL Buffer R2 (STEMCELL Technologies ...
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ER mediates spatial regulation of lysosome-endosome interactions via motion switch at junction sitesbioRxiv - Cell Biology 2023Quote: ... The shRNA plasmids were constructed based on the lentiviral backbone PLKO.1 (Addgene Cat# 8453) following the protocol provided by Addgene (https://www.addgene.org/protocols/) ...
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bioRxiv - Cell Biology 2024Quote: ... Constructs to KD Slug in SGEF KD were cloned into pLKO.1-Hygro (Addgene 24150). To generate Scribble and Dlg1 CRISPR/Cas9 KO cell lines we used pLentiCRISPR v2-Blast (Addgene 83480) ...
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bioRxiv - Microbiology 2023Quote: ... Each well was then transfected with the lentiviral packaging vectors psPAX2 (1 µg, Addgene, 12260), and pMD2.G (1 µg ...
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bioRxiv - Biochemistry 2023Quote: ... coli BL21 (DE3) cells using the plasmid pRSFDuet-1/CylLL/CylM-2 (Addgene ID #208759), following the method previously reported.9 For the preparation of lanthionine standard ...
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bioRxiv - Neuroscience 2023Quote: ... and pAAV-syn-FLEX-splitTVA-EGFP- tTA (diluted 1:200 in dPBS; Addgene: 100798-AAV1) was injected into basal forebrain (AP ...
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bioRxiv - Immunology 2023Quote: ... stably expressing clonal RAW MΦs were transduced with pLenti CMV Puro DEST (Addgene w118-1) constructs containing 3xFL-GFP ...
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bioRxiv - Neuroscience 2024Quote: ... and C1(1-29)-TurboID-V5_pCDNA3 vectors were obtained from Addgene (149414, 149415, 166971, 107173).58–60 Each was separately inserted into the pZac2.1 GfaABC1D-tdTomato vector109 obtained from Addgene (44332 ...
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bioRxiv - Molecular Biology 2024Quote: Oligoes containing shRNA target sequences were cloned into pLKO.1-blast lentiviral vector (Addgene #26655). shRNA sequences used in this study are listed below.
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bioRxiv - Neuroscience 2024Quote: ... mice were injected in LHA with AAV5-hSyn-DIO-eGFP (1*10^12vc/ml; Addgene) and co-injected with rAAV5-ORXpr1-3TdTomato (1*10^12 gc/ml ...
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bioRxiv - Neuroscience 2024Quote: ... DV - 3.8) with 200 nL AAV9.EF1a.dflox.hChR2(H134R).mCherry.WPRE.HGH (1×10^12 pp/mL, Addgene). For subjects in cohort 1 (n=2) ...
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bioRxiv - Biochemistry 2024Quote: ... The tag-free wildtype (Wuhan-hu-1) nucleocapsid protein plasmid was purchased from Addgene (#177937), and the plasmid encoding the Strep-tagged nucleocapsid protein was a kind gift from Dr ...
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bioRxiv - Cancer Biology 2024Quote: ... Scramble pLKO.1 shRNA control (further termed “scramble”) was a gift from David Sabatini (Addgene plasmid #1864 ...
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bioRxiv - Bioengineering 2019Quote: cDNA for pLenti CMV GFP Puro (658-5) was a gift from Eric Campeau and Paul Kaufman (Addgene plasmid #1744856). To generate lentivirus ...
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bioRxiv - Cell Biology 2020Quote: ... designed by Life technologies to target the second exon of murine Vangl2 (gRNA: 5’-TCGGCTATTCCTACAAGTC-3’) into pSpCas9(BB)-2A-GFP (PX458, Addgene #48138). Upon transfection ...
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bioRxiv - Developmental Biology 2021Quote: ... The following guides 5’-GGACCTGTTCGGAATCCACC-3’ and 5’-GGGTGAGGTTCTGTCTACCC-3 were separately cloned into the BbsI site of pU6-BbsI-chiRNA plasmid (obtained from Addgene) and both were simultaneously injected by Best Gene into w1118 ...
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bioRxiv - Molecular Biology 2020Quote: The gRNA sequence: 5’-TTAAAGAGTAACTACCAACT-3’ targeting the mouse Xpo7 gene was cloned into the PX459 plasmid (Addgene #62988, (23)) ...