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Citations for Addgene :
1001 - 1050 of 2227 citations for 8 phenylamino 5 4 5 sulpho 1 naphthyl azo 1 naphthyl azo naphthalene 1 sulphonic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: ... with 1 μg of DNA plasmid vector Cry2Olig-mCherry (purchased from Addgene, number 60032), and then platted on fluorodishes ...
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bioRxiv - Molecular Biology 2021Quote: ... lentiviruses were produced in HEK293T cells in a pLKO.1-puro (Addgene, plasmid 8453) backbone ...
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bioRxiv - Genomics 2021Quote: ... IMR90 cells were transfected with 1 µg of pEGFP-Δ50 LMNA (Addgene Plasmid #17653). After expression of the GFP was observed (∼2 days) ...
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bioRxiv - Plant Biology 2021Quote: ... pENTR4-GFP-C3 (w393-1) was a gift from Eric Campeau & Paul Kaufman (Addgene plasmid # 17397 ...
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bioRxiv - Developmental Biology 2020Quote: ... and corresponding 1-kbp homology arms were cloned into pHD vector (pHD-DsRed, Addgene plasmid #51434 ...
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bioRxiv - Neuroscience 2021Quote: ... 1 million cells were nucleofected with iμg pAAV-CAG-GFP plasmid DNA (Addgene 37825) in Ingenio electroporation buffer (Mirus MIR50111 ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 µg of the resulting plasmid and 0.5 µg of plasmid hCas9 (Addgene 41815) were co-transfected using 3 µl of Turbofect transfection reagent (Thermo Fisher) ...
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Optimized RNA-targeting CRISPR/Cas13d technology outperforms shRNA in identifying essential circRNAsbioRxiv - Molecular Biology 2020Quote: ... The oligonucleotides for shRNA were cloned into the pLKO.1-TRC vector (Addgene #10878) using AgeI/EcoRI ...
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Evolutionary plasticity of SH3 domain binding by Nef proteins of the HIV-1/SIVcpz lentiviral lineagebioRxiv - Microbiology 2021Quote: ... and HIV-1 P RBF168) were cloned into the pWPI-GFP vector (Addgene # 12254) to transduce Jurkat cells stably expressing CD4.
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bioRxiv - Cancer Biology 2020Quote: ... The shRNA sequences were assembled into a pLKO.1 lentiviral backbone (Addgene plasmid #10878), containing a puromycin resistance marker to allow for the antibiotic selection of transduced cells ...
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bioRxiv - Neuroscience 2021Quote: ... The pLKO.1 TRC cloning vector was a gift from David Root (RRID: Addgene_10878) (Moffat et al. ...
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bioRxiv - Biochemistry 2022Quote: Human PARP6 (Uniprot #Q2NL67-1) was cloned into a modified pFASTBac1 vector (Addgene #30116) with N-terminal 6X His-maltose binding protein (MBP ...
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bioRxiv - Cancer Biology 2022Quote: ... oligonucleotides of the same sequence (TRCN0000111876) were cloned into pLKO.1-blast (Addgene #26655). Cells transduced were selected in culture containing puromycin (InvivoGen ...
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bioRxiv - Cancer Biology 2022Quote: ... β-catenin shRNA was purchased from Addgene (pLKO.1 puro shRNA β-catenin #18803). Scramble siRNA and p68 siRNA were purchased from Santa Cruz Biotechnology (Santa Cruz ...
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bioRxiv - Neuroscience 2022Quote: ... a volume of 1-1.2 μl of the viral plasmid pAAV.hSyn.Flex.iGluSnFR.WPRE.SV40 (gift from Dr. Loren Looger; Addgene plasmid # 98931 ...
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bioRxiv - Neuroscience 2022Quote: ... titer: 4.5 × 1013 GC/ml (Cat # AV-1-ALL864, RRID:Addgene_51503; U Penn Vector Core).
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bioRxiv - Neuroscience 2022Quote: ... 10 μL of AAV-PHPeB-hsyn-cre-eGFP (Addgene#105540, titer ∼1×10^13) or AAV-PHPeB-CAG-GFP (Addgene#37825 ...
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bioRxiv - Synthetic Biology 2022Quote: ... we have combined them all in a pX330A-dCas9 1×6 (Plasmid #63600, Addgene). Cloning of oligonucleotides was confirmed by DNA sequencing ...
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bioRxiv - Cancer Biology 2023Quote: ... 2μg of target shRNA construct and 2μg of 3:1 ratio of psPAX2 (Addgene) and pMD2.G (Addgene ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Cells were transiently transfected with 1 μg of each plasmid expressing GCaMP6s (Addgene #277314.1040753) (Chen et al. ...
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bioRxiv - Neuroscience 2024Quote: ... 1:20 of final mix) was mixed with AAV5-hSyn-DIO-mCherry-WPRE (Addgene 50459 ...
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bioRxiv - Neuroscience 2024Quote: ... we unilaterally injected 450 nL AAV9-hSyn-flex-GCaMP7s (≥ 1×10¹³ vg/mL, Addgene)107 into lAcbSh ...
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bioRxiv - Cancer Biology 2024Quote: ... Nontargeting shRNA in pLKO.1 (shSCR) was used as a control (Addgene Plasmid #17920).
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bioRxiv - Cell Biology 2024Quote: ... subsequently Dm-KHC(1-421)-SNAP-6xHis (gift from Kapitein lab, Addgene plasmid #196976) labelled with Alexa647-SNAP dye (NEB ...
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bioRxiv - Genetics 2024Quote: ... 1 μg of the lentiviral transfer vector G1088E_pLenti-CMV-mNeonGreen-2A-HygroR (Addgene #216279), and 1 μg of one of six spike proteins tested.
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Ribonuclease Inhibitor and Angiogenin collaboratively regulate cell-type-specific global translationbioRxiv - Biochemistry 2024Quote: ... and then recombined into the destination vector pLenti CMV Blast DEST (706-1, Addgene) plasmid using the Gateway cloning method.
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bioRxiv - Cell Biology 2024Quote: ... and pLKO.1 ShCtrl and psPAX2 (#12260) and pMD2G (#12259) (Addgene, Cambridge, MA, USA) were used ...
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bioRxiv - Cell Biology 2024Quote: ... or pLKO.1-Neo-CMV-tGFP-Scramble shRNA plasmid (Addgene#136035 as scramble control). Lentiviral vectors were packaged using packaging plasmid (pCMV delta R8.2 dvpr ...
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bioRxiv - Molecular Biology 2023Quote: ... we cloned the shRNA targeting TET2 into the pLKO.1-blast vector (Addgene #26655). Plasmids were generated using PCR ...
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bioRxiv - Neuroscience 2023Quote: ... AAV2/1-EF1a-DIO-mKate2-PDE4D3-Cat (Boston Children’s Hospital Vector Core; Addgene 169128) or AAV9-Ef1a-DIO-mCherry (UNC Vector core ...
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bioRxiv - Neuroscience 2023Quote: ... AAV2/1-EF1a-DIO-mKate2-PDE4D3-Cat (Boston Children’s Hospital Vector Core; Addgene 169128), and AAV2/1-hSyn-DIO-GreenDownwardcADDis (Boston Children’s Hospital Vector Core ...
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bioRxiv - Cancer Biology 2022Quote: ECs were transfected with 1 µg of a PGC1α promoter luciferase (Addgene # Plasmid #8888) and 35 ng of pRL/TK using PEI transfection reagent (Polyethylenimine) ...
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bioRxiv - Cell Biology 2022Quote: ... plasmids from the step 1 were cloned into pLV GG hUbC-EGFP (84034, Addgene; dsRED was replaced with EGFP from 1168 ...
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bioRxiv - Genomics 2022Quote: ... gRNAs were constructed from pSLQ2853-3 pHR: U6-Sasgv2CXCR4-1 CMV-EGFP (Addgene 84254) and pSLQ1852-2 pHR ...
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bioRxiv - Neuroscience 2023Quote: ... and CamKII-Cre (pENN.AAV.CamKII 0.4.Cre.SV40, titer≥1×10¹³ vg/ml Addgene 105558-AAV9) virus was injected in S1 barrel cortex ...
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bioRxiv - Neuroscience 2023Quote: ... AAV2/1-EF1a-DIO-mKate2-PDE4D3-Cat (Boston Children’s Hospital Vector Core; Addgene 169128) or AAV9-Ef1a-DIO-mCherry (UNC Vector core ...
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bioRxiv - Neuroscience 2023Quote: ... AAV2/1-EF1a-DIO-mKate2-PDE4D3-Cat (Boston Children’s Hospital Vector Core; Addgene 169128) and AAV1-Syn-Flex-GCaMP6s-WPRE-SV40 (Addgene 100845-AAV1 ...
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bioRxiv - Developmental Biology 2023Quote: ... and hnd-1p::ZIF-1 (pRA642) expression constructs were created in pCFJ355 (Addgene #34870), a vector designed for Mos1-mediated single copy transgene insertion on LGX (Frokjaer-Jensen et al ...
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bioRxiv - Neuroscience 2023Quote: ... 0.75 µL of rgAAV-FLEX-tdTomato (diluted 1:3 in dPBS; Addgene number: 28306) was injected in TH-Cre mice in basal forebrain (AP ...
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bioRxiv - Neuroscience 2023Quote: ... 1 µL of a virus encoding AAV5.CamKIIa.GCaMP6f (pENN.AAV.CamKII.GCaMP6f.WPRE.SV40, Addgene viral prep 100834-AAV5) virus was injected unilaterally in the PL (AP +1.9 ...
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bioRxiv - Neuroscience 2023Quote: ... Ascl1 was cloned into pLenti CMV/TO Neo empty (w215-1) plasmid (Addgene #17485).
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bioRxiv - Microbiology 2023Quote: ... plus the pscALPS-hACE2 or -cACE2 (1 µg) or the empty pscALPS_puro (Addgene, 128504) vector as a negative control ...
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bioRxiv - Cell Biology 2023Quote: ... For retroviral transfections 1 μg VSVG and 1.86 μg HIV gag-pol (Addgene #14887) were used ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were then transfected with 1 μg of pHrD-IRES-Fluc (Addgene plasmid #194250) and 1 ng of CMV-Rluc reporter plasmids (78 ...
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bioRxiv - Neuroscience 2024Quote: ... human TDP-43M337V has subcloned into the pLenti CMV Puro DEST (W118-1, Addgene) plasmid as previously described (Zhang et al ...
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bioRxiv - Biophysics 2024Quote: ... A plasmid encoding aa 1-261 of human utrophin (PaGFP-UtrCH, Addgene plasmid #26738) was obtained as a gift from Dr ...
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bioRxiv - Physiology 2021Quote: ... Adeno-associated virus serotype 8 (AAV8) with the thyroxine-binding globulin promoter (TBG) promoter expressing Cre (Addgene, AV-8-PV1091) were injected via tail vein at a dose of 1×1012 genomic copies per mouse on GD8 ...
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bioRxiv - Immunology 2023Quote: ... were transfected with the above retroviral plasmids encoding the 8-24 or 8-DN TCRβ transgene and the pCL-Eco packaging vector (Addgene) using Lipofectamine 2000 (Life Technologies ...
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bioRxiv - Developmental Biology 2020Quote: The zebrafish gfap promoter 51 was derived from the Addgene plasmid: pEGFP-gfap(Intron1/5’/Exon1-zebrafish) (Addgene, #39761) and lssmKate2 65 was derived from the Addgene plasmid ...
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bioRxiv - Neuroscience 2021Quote: ... and sg-KCTD16-2 (5’-TTCCGCAAACCAAAATCCGG-3’) were then cloned into the AAV-U6-sgRNA-hSyn- mCherry plasmid (RRID:Addgene_87916) using the SapI site 5’ of the gRNA scaffold ...