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Citations for Addgene :
51 - 67 of 67 citations for pET28a DsRed2 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: A pET28a plasmid containing the SENP2 catalytic domain (cat.SENP2) was obtained from Addgene (Catalog number 16357).44 The protein was expressed and purified from E ...
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bioRxiv - Bioengineering 2024Quote: ... pET28a-MBPX-MBPX-SpyTag003 (His6-MBPX-SSSGGSGGGSG linker-MBPX-SpyTag003; GenBank and Addgene deposition in progress), where MBPX is a variant of MBP with stronger maltose binding (Veggiani et al. ...
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bioRxiv - Synthetic Biology 2022Quote: ... The Spy and Snoop catcher domains were cloned from the pET28a-SpyCatcher-SnoopCatcher plasmid (Addgene, #72324) while the Traptavidin domain came from pET21a-Core-Traptavidin (Addgene ...
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bioRxiv - Plant Biology 2020Quote: ... the Hip1 sequence was amplified without signal peptide and cloned into the pET28a (+) expression vector (Addgene, USA), eventually having a His-tag at the N-terminus ...
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bioRxiv - Biochemistry 2022Quote: A pET28a plasmid containing full-length human β-catenin was gifted from Randall Moon (Addgene plasmid # 17198) and various fragments of the coding region (residues 1-137 (NTERM) ...
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bioRxiv - Cell Biology 2023Quote: The bacterial expression vector for pET28a-EGFP-CNA35 was a gift from Maarten Merkx (Addgene plasmid # 61603; http://n2t.net/addgene:61603; RRID:Addgene_61603) (50) ...
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bioRxiv - Molecular Biology 2024Quote: ... and pET28a(+)6xHis-TEV-IFIT3 were a gift from Kathleen Collins (Addgene plasmid # 53557; http://n2t.net/addgene:53557; RRID:Addgene_53557 ...
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bioRxiv - Biochemistry 2022Quote: The pET28a plasmid vector containing DNA sequences encoding the PANK3 protein (residues pro12 to Asn368) was purchased from Addgene (25518) and transformed into both E ...
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bioRxiv - Biochemistry 2023Quote: Dephosphorylated AurA (residues 122-403, TEV-cleavable, N-terminal His6-tagged, kanamycin-resistance) in pET28a and LPP (#79748) from Addgene were cotransformed in BL21(DE3 ...
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bioRxiv - Neuroscience 2022Quote: ... A photoactivatable adenylyl cyclase (PAC) from Beggiatoa sp (amplified from pET28a-ec_bPAC, a gift from Peter Hegemann (Addgene plasmid # 28135)) or empty vector control was transgenically expressed in the egg-laying muscles of C ...
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bioRxiv - Bioengineering 2024Quote: ... pET28a-SnoopTag-AffiHER2-SpyTag (N-terminal His6–SnoopTag–anti-HER2 Affibody–SpyTag) (GenBank accession no. KU296975) (Addgene deposition in progress) (Brune et al. ...
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bioRxiv - Biochemistry 2023Quote: ... the open reading frame of QBP was amplified from E.coli K12 DNA and cloned into bacterial expression vector pET28a-LIC (Addgene #26094). Transformed E.coli BL21 (DE3) ...
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bioRxiv - Cell Biology 2024Quote: ... cells were co-transformed with pET28a plasmid encoding the amber mutant TTC5 and the pEVOL-pBpF plasmid (Addgene plasmid #31190). Cells were grown at 37 °C in LB containing 50 μg/mL kanamycin and 25 μg/mL chloramphenicol and induced with 0.2 % L-arabinose at an A600 of 0.3 for 30 min followed by a second induction with 0.2 mM IPTG at an A600 of ∼0.6 at 16 °C overnight ...
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bioRxiv - Biochemistry 2024Quote: ... DNA sequences for 8xHis-MBP TRPA11089-1119 and 3xFlag-6xHis-WT CaM were PCR amplified and subcloned into a pET28a vector (Addgene) at the NcoI/NotI sites using In-Fusion EcoDry cloning ...
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bioRxiv - Biophysics 2020Quote: ... was expressed in E.coli using gene with an N-terminal 6XHis-tag and up stream TEV-protease site cloned into pET28a(+) (Addgene plasmid #2006150). MSP1D1 was purified using IMAC72 with further cleavage of 6xHis-tag by TEV protease (Sigma-Aldrich) ...
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bioRxiv - Biochemistry 2023Quote: ... and the 6xHis-SUMO tag was removed by enzymatic cleavage using human Sentrin-specific protease 1 (SENP1) catalytic domain (derived from pET28a-HsSENP1, that was a gift from Jorge Eduardo Azevedo (Addgene plasmid #71465) at 4°C overnight27,28 ...
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bioRxiv - Biophysics 2023Quote: ... was expressed in E.coli using a gene with an N-terminal 6×His-tag and an upstream TEV-protease site cloned into pET28a(+) (Addgene plasmid #20061). MSP1D1 was purified using IMAC with further cleavage of 6×His-tag by TEV protease 50,51 ...