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Citations for Addgene :
1 - 50 of 67 citations for pET28a DsRed2 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... and DsRed2 ER5 (Addgene plasmid 55836) originated from Dr ...
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bioRxiv - Synthetic Biology 2023Quote: ... We then replaced the mCerulean gene carried in the new P3 plasmid first with the dsRed2 gene amplified from the CMVp-dsRed2-Triplex-28-gRNA1-28 (Addgene cat no. 55200) into the FseI-treated P3-7xTS by restriction-ligation to create P3-7xTS-dsRed2 ...
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bioRxiv - Genetics 2022Quote: pENTR-DsRed2 N1 (CMB1) was a gift from Eric Campeau (Addgene plasmid #22523 ...
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bioRxiv - Cell Biology 2020Quote: ... and pET28a(+) (Addgene #69864-3), for His6-tag protein purification ...
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bioRxiv - Cell Biology 2024Quote: The plasmid EF1a-mito-dsRED2 was a gift from Thomas Schwarz (Addgene plasmid # 174541 ...
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bioRxiv - Physiology 2022Quote: ... cells were transfected with 1μg DsRed2-Mito-7 (Mito-dsRed) (Addgene Plasmid #55838) by lipofectamine (Invitrogen L3000008 ...
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bioRxiv - Synthetic Biology 2023Quote: ... inverse PCR from CMVp-dsRed2-Triplex-28-gRNA1-28 (Addgene cat no. 55200) using a pair of primers including SacI restriction sites generated a linear plasmid ...
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bioRxiv - Biophysics 2021Quote: ... MSP1D1 in pET28a was purchased from Addgene and was expressed and purified as previously described(58).
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bioRxiv - Biophysics 2022Quote: ... was cloned into a pET28a vector (Addgene) and transformed in E ...
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bioRxiv - Biophysics 2020Quote: ... MSP2N2 inserted into a pET28a vector (Addgene) was expressed in BL21 (DE3 ...
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bioRxiv - Immunology 2020Quote: The pET28a His6-SpyCatcher-mi3 gene (Addgene) was transformed into BL21 (DE3)-RIPL E.coli (Agilent) ...
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bioRxiv - Biochemistry 2023Quote: ... from pET28a/Cas9-Cys (Addgene plasmid # 53261), which was kindly provided by Hyongbum Kim via Addgene ...
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bioRxiv - Microbiology 2024Quote: pET28a-SpyCatcher003-mi3 (GenBank MT945417, Addgene 159995) (Rahikainen et al. ...
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bioRxiv - Immunology 2024Quote: pET28a-SpyCatcher003-mi3 (GenBank MT945417, Addgene 159995) was previously described23 ...
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bioRxiv - Biochemistry 2020Quote: ... The pET28a-LIC-HAT1 plasmid (plasmid #25239, Addgene) was transformed into BL21 (DE3)/ RIL competent cells through heat-shock and then spread on agar plates containing antibiotics kanamycin and chloramphenicol ...
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bioRxiv - Genetics 2023Quote: ... xiap and p47 were cloned in DsRed2-N1(a gift from Michael Davidson, Addgene plasmid #54493) and pAQUA-N1vectors (a gift from Fabienne Merola ...
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bioRxiv - Immunology 2020Quote: ... pET28a-SpyCatcher003-mi3 (GenBank and Addgene deposition in progress) was created by replacing SpyCatcher in pET28a-SpyCatcher-mi3 with SpyCatcher003 18 ...
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bioRxiv - Microbiology 2022Quote: ... transformed with the pET28a His6-SpyCatcher003-mi3 gene (Addgene) as described (34 ...
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bioRxiv - Bioengineering 2024Quote: ... pET28a-nanoHER2-SpyTag003 (GenBank and Addgene deposition in progress), based on the 11A4 nanobody against HER2 (Verheije et al. ...
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bioRxiv - Biophysics 2023Quote: The pET28a-EGFP-CNA35 DNA construct purchased from Addgene was described before(Aper et al. ...
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bioRxiv - Microbiology 2024Quote: ... and pET28a-AviTag-DogCatcher-MBP (GenBank MZ365293, Addgene 171928) (Keeble et al. ...
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bioRxiv - Microbiology 2024Quote: ... from the pET28a-C-tag-SpyCatcher-Mi3 (Addgene #112255) and pET15b-StrepTag-SpyCatcher-LS (Novagen ...
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bioRxiv - Genetics 2022Quote: pENTR-DsRed2 N1 (CMB1) was a gift from Eric Campeau (Addgene plasmid #22523; http://n2t.net/addgene:22523; RRID:Addgene_22523). pDONR223_GLI1_WT was a gift from Jesse Boehm & William Hahn & David Root (Addgene plasmid #82123 ...
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bioRxiv - Cell Biology 2024Quote: The plasmid EF1a-mito-dsRED2 was a gift from Thomas Schwarz (Addgene plasmid # 174541; http://n2t.net/addgene:174541; RRID:Addgene_174541). It contains a Flex-Switch-MitoDsRed construct under the transcriptional control of the EF-1α promoter ...
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bioRxiv - Biochemistry 2020Quote: ... coli BL21-CodonPlus (DE3) cells containing pET28a-MSP1D1delltaH5 vector (Addgene cat# 71714 was kindly purchased by Dr ...
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bioRxiv - Biochemistry 2024Quote: ... The pET28a-TRBP was purchased from Addgene (Addgene plasmid # 50351). The pET28a-TRBP plasmid was transformed into E ...
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bioRxiv - Cell Biology 2022Quote: Constructs used were: Mito-DsRed2 (kindly provided by T. Schwarz, Harvard Medical School, Boston) and Mito-SBFP2 (Addgene #187964); untagged Parkin (Addgene #187897) ...
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bioRxiv - Developmental Biology 2021Quote: ... SpCas9 was expressed from pET28a-Cas9-His (Addgene plasmid number 98158) and purified in our lab ...
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bioRxiv - Cell Biology 2021Quote: Protein expression vector pET28a-mCherry-CNA35 was obtained from Addgene (#61607). Transformed E.coli (BL21 ...
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bioRxiv - Bioengineering 2024Quote: pET28a-SpyTag003-MBP (GenBank Accession no. MN433888, Addgene plasmid ID 133450), pET28-SpyTag003-mClover3 (Addgene plasmid ID 133453 ...
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bioRxiv - Developmental Biology 2023Quote: ... coli colonies with the pET28a-EGFP-CNA35 gene (Addgene, Plasmid #61603) were cultured in LB media and protein expression was induced with 0.5 mM isopropyl β-D-1-thiogalactopyranoside (Sigma Aldrich ...
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bioRxiv - Bioengineering 2024Quote: ... Coli BL21 strain harboring pET28a-Cas9-His were purchased from Addgene Inc ...
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bioRxiv - Developmental Biology 2022Quote: ... pET28a-mCherry-CNA35 was a gift from Maarten Merkx (Addgene plasmid # 61607)20 ...
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bioRxiv - Cell Biology 2023Quote: ... by replacement of the eGFP coding sequence within pET28a-eGFP-CNA3546 (Addgene, #61603 ...
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bioRxiv - Molecular Biology 2024Quote: ... and pET28a(+)6xHis-TEV-IFIT3 were a gift from Kathleen Collins (Addgene plasmid # 53557 ...
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bioRxiv - Neuroscience 2020Quote: ... DCC:TDTOMATO (pmDCC:TDTOMATO; provided by Erik Dent, University of Wisconsin-Madison) was subcloned into the pCag-DsRed2 plasmid (Addgene, 15777, Cambridge, MA), by excising DsRed2.
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bioRxiv - Bioengineering 2022Quote: ... and the dsRed filler fragment was generated by amplifying a portion of the dsRed gene from CMVp-dsRed2-Triplex-HHRibo-gRNA1-HDVRibo-pA (Addgene 55201) with primer set AA-sg1-scaf-short-filler-FW/AA-filler-SpeI-gib-RV in separate PCR reactions ...
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bioRxiv - Synthetic Biology 2023Quote: ... the CMV promoter of the dsRed2-free plasmid was replaced with the EF1α promoter amplified from pCEP4_WT_OCT4 (Addgene cat no. 40629) by Gibson assembly after KpnI and SacI restriction ...
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bioRxiv - Synthetic Biology 2022Quote: ... SpyTag and SnoopTag sequences were obtained from pET28a-SnoopTag-mEGFP-SpyTag (Addgene, #72325), while the AviTag sequence originated from AviTag-SpyCatcher plasmid (Addgene ...
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bioRxiv - Biochemistry 2023Quote: ... The pET28a plasmid carrying the MSP1E1 gene was obtained from Addgene (plasmid #20062). The membrane scaffold protein (MSP1E1 ...
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bioRxiv - Cancer Biology 2023Quote: The plasmid pet28a-His6-Keap1 for expressing His-KEAP1 was purchased from Addgene (a gift from Yimon Aye ...
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bioRxiv - Cancer Biology 2023Quote: ... coli cells were transformed with the pET28a-His6-KEAP1 vector (Addgene plasmid #62454). Colonies were grown in LB broth containing 50 μM ZnCl2 and 50 μg/ml kanamycin at 37 °C until cultures reached an OD600 of 0.6-0.8 and then were allowed to cool to room temperature ...
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bioRxiv - Synthetic Biology 2024Quote: ... The TdT gene was cloned into a pET28a expression vector (Addgene #69864-3) with an N-terminal 10xHis tag ...
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bioRxiv - Biochemistry 2024Quote: ... The expression and purification of MOZ protein (pET28a-LIC-MOZ plasmid, Addgene #25181) were done according to the protocol from Structural Genomics Consortium (SGC ...
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bioRxiv - Biophysics 2020Quote: The plasmid for membrane scaffold protein was MSP1D1 in pET28a was purchased from Addgene and prepared as previously described (Hagn et al. ...
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bioRxiv - Bioengineering 2022Quote: ... the pET28a-EGFP-CNA35 plasmid was received as a gift from Maarten Merkx (Addgene plasmid # 61603 ...
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bioRxiv - Biochemistry 2021Quote: ... elegans ERH-2 (NCBI gene ID: 185323) was cloned into pET28a (Addgene: 69864-3), containing an N-terminal His6 tag followed by a TEV protease cleavage site (MGSSHHHHHHSSGENLYFQGHMAS ...
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bioRxiv - Bioengineering 2024Quote: ... and pET28a-SnoopTag-SpyTag-(AffiHER2)3 (GenBank accession no. KU296976) (Addgene deposition in progress) (Veggiani et al. ...
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bioRxiv - Cell Biology 2023Quote: The bacterial expression vector for pET28a-EGFP-CNA35 was a gift from Maarten Merkx (Addgene plasmid # 61603 ...
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bioRxiv - Microbiology 2024Quote: ... tuberculosis H37Rv ΔRD1 ΔpanCD genomic DNA by PCR and ligated onto the pET28a vector (Addgene) that had been digested with the NcoI and XhoI restriction enzymes (Thermo Fisher) ...