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Citations for Addgene :
51 - 100 of 1485 citations for Astrovirus Antigen Type 1 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2019Quote: Arachnoid cells were immortalized using a lentivirus harboring the SV40 large T antigen [pBABE-puro SV40 LT was a gift from Thomas Roberts (Addgene, RRID:Addgene_13970) as previously described (48) ...
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bioRxiv - Molecular Biology 2022Quote: ... early passage primary MEFs from all genotypes were infected in parallel with the SV40 large T antigen (Addgene:13970) (Zhao et al. ...
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bioRxiv - Cell Biology 2022Quote: ... cDNA encoding human Dnm1 wild-type was amplified from Dnm1-pmCherryN1 (Addgene #27697 ...
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bioRxiv - Cancer Biology 2022Quote: ... pCDNA3-V5-PTPN14-wild type was a gift from Jianmin Zhang (Addgene plasmid # 61003 ...
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bioRxiv - Molecular Biology 2023Quote: ... The type V Scytonema hofmanni (Sh)CAST was obtained from Addgene (#127922) [15] ...
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bioRxiv - Biochemistry 2023Quote: ... Human codon optimized wild-type Mpro was obtained from Addgene (catalog #141370). Mpro single point mutants (M49A ...
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bioRxiv - Neuroscience 2024Quote: Adeno-associated virus (AAV) type 2 carrying cre-dependent ChR2-eYFP (Addgene plasmid 20298 ...
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bioRxiv - Biophysics 2021Quote: ... and immortalized by transduction with a retrovirus expressing SV40 T antigen from pBABE-puro SV40LT (pBABE-puro SV40LT was a gift from Thomas Roberts (Addgene plasmid # 13970 ...
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bioRxiv - Cancer Biology 2024Quote: The chimeric antigen receptor against human CD276 (Du et al., 2019) and CD19 (Maude et al., 2018)together with the Thy1.1 surface antigen (Addgene 154194)(Umkehrer et al. ...
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bioRxiv - Cell Biology 2023Quote: ... Non-GFP expressing ZT cells were obtained by sorting out non-fluorescence ZT cells and they were subsequently immortalized by transfecting them with SV40 T-antigen expressing plasmid (Addgene).
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bioRxiv - Cancer Biology 2022Quote: TP53 constructs (wild-type, G245D, R175H) in pBabe retroviral expression vector (pBABEpuro; Addgene) were a generous gift from Drs ...
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bioRxiv - Biochemistry 2021Quote: ... was inserted into a vector PX601-containing wild-type SaCas9 (Addgene plasmid #61591) and replacing that domain of SaCas9 ...
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bioRxiv - Cancer Biology 2022Quote: ... PSMC3IP p.Glu201del or wild-type PSMC3IP cDNA was cloned into pLX302 (Addgene #25896) expression vector ...
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bioRxiv - Genetics 2023Quote: ... These plasmids were co-transfected with wild-type SpCas9 (RTW3027, Addgene plasmid # 139987) or the SpG variant capable of targeting sites encoding NGA PAMs (RTW4177 ...
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bioRxiv - Cancer Biology 2019Quote: Arachnoid cells were immortalized using a lentivirus harboring the SV40 large T antigen [pBABE-puro SV40 LT was a gift from Thomas Roberts (Addgene, RRID:Addgene_13970) as previously described (48) ...
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bioRxiv - Biochemistry 2020Quote: ... wild-type and mutant 3xFLAG-CDK12 sequences were subcloned into lentiCas9-Blast (Addgene 52962) by replacing the Cas9 ORF with 3xFLAG-CDK12 ...
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bioRxiv - Molecular Biology 2023Quote: ... wild-type MEFs were transduced with lentiviral particles containing the plasmids lentiMPHv2 (Addgene #89308) and lentiSAMv2 (Addgene #75112) ...
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bioRxiv - Molecular Biology 2023Quote: ... WT and T1150A catalytic domains tagged with nuclear localization sequence (NLS) of the SV40 Large T-antigen were cloned into the mVenus-C1 plasmid backbone (provided by Steven Vogel, Addgene plasmids no. 27794) (Koushik et al. ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were immortalized by transduction with a retrovirus expressing SV40 large T antigen from pBABE-puro largeTcDNA (Addgene plasmid # 14088; http://n2t.net/addgene:14088; RRID:Addgene_14088; a gift from William Hahn) as previously described (13) ...
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bioRxiv - Cancer Biology 2019Quote: ... Wild-type human CDH1 on a pcDNA3 plasmid was obtained (hE-cadherin-pcDNA3, Addgene, 45769). Variants were generated using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs) ...
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bioRxiv - Molecular Biology 2020Quote: ... His-tagged wild-type and D135S AlkB plasmids were obtained from Addgene (#79050 and #79051) and proteins were purified by a Ni-NTA column followed by cation exchange ...
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bioRxiv - Cell Biology 2020Quote: ... Wild-type β-PheRS cDNAs were cloned into the pET LIC (2A-T) plasmid (Addgene). Then ...
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bioRxiv - Cancer Biology 2019Quote: ... The CRISPR/Cas9 plasmid encoding the target wild type sgKRAS sequence was purchased from Addgene. CRISPR/Cas9 plasmid at 2 μg concentration was transfected by Turbofectin 8.0 following the protocol from OriGene (Rockville ...
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bioRxiv - Cell Biology 2022Quote: Full-length ERK3 wild type (WT) pDONR-223 construct purified from Human Kinase Library (Addgene) was used as a template to generate ERK3 K49A K50A kinase dead (KD ...
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bioRxiv - Cell Biology 2022Quote: pHAGE-PIK3CA encoding PIK3CA wild-type (wt; Addgene plasmid #116771; http://n2t.net/addgene:116771; RRID:Addgene_116771) and pHAGE-PIK3CA-H1047R encoding PIK3CA with oncogenic point mutation on p.H1047R (Addgene plasmid #116500 ...
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bioRxiv - Molecular Biology 2023Quote: ... The type I-B Anabaena variabilis ATCC 29413 CAST was sub-cloned from Addgene (#168137) [16] ...
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bioRxiv - Cancer Biology 2020Quote: ... wild-type HeLa and MCF10A cells were infected with the dCas9 lentiviral construct (Addgene 61425-LV) and selected with 3μg/ml blasticidin for 5 days ...
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bioRxiv - Biochemistry 2021Quote: URA7 and URA8 wild type genes with ribosomal binding site were cloned into pet28b-6His (Addgene, Massachusetts ...
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bioRxiv - Cancer Biology 2023Quote: ... wild type TP53 cDNA was amplified from p53-GFP plasmid (Cat #11770) (Addgene, Cambridge, Massachusetts, USA) and cloned into pFN21A (Promega Corporation ...
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bioRxiv - Cell Biology 2023Quote: ... Wild-type and Fam134s KO MEFs were infected with pCW57-CMV-ssRFP-GFP-KDEL (Addgene #128257) in order to generate ER-phagy reporter inducible cell lines ...
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bioRxiv - Cancer Biology 2020Quote: ... Human codon-optimized Streptococcus pyogenes wild type Cas9 (Cas9-2A-GFP) was obtained from Addgene (Cambridge, MA). Chimeric guide RNA expression cassettes with different sgRNAs (sgRNA1 ...
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bioRxiv - Cell Biology 2020Quote: The doxycycline-inducible PLK4 plasmid was generated by PCR of the wild-type PLK4 cDNA from Addgene plasmid #41165 by PfuUltra II Fusion HS DNA polymerase (Agilent #600670. ...
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bioRxiv - Bioengineering 2020Quote: ... either of the two cell types was subjected to Lipofectamine transfection with CMV-R-GECO1.2-mCherry (Addgene, Watertown ...
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bioRxiv - Synthetic Biology 2021Quote: ... mTiam1(64-437)-tgRFP-SspB wild type were a gift from Brian Kuhlman (Addgene plasmid # 60411; http://n2t.net/addgene:60411; RRID:Addgene_60411, and Addgene plasmid # 60417 ...
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bioRxiv - Microbiology 2020Quote: Plasmids for lentiviral transduction (pLJM1-2xStrep or untagged wild-type ORF68, mutants, and homologs) (Addgene #x-x) were generated by subcloning into the AgeI and EcoRI sites of pLJM1 modified to confer resistance to zeocin (Addgene #19319 ...
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bioRxiv - Neuroscience 2022Quote: ... wild-type embryos were injected with a vsx1:Gal4 BAC and UAS:CoChR2-tdTomato plasmid (Addgene Catalog #: 124233) at 20 ng/µL and 25 ng/µL ...
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bioRxiv - Genomics 2022Quote: ... Wild-type (J1) mESCs were transduced with Cas9-blast virus (generated from pLentiCas9-Blast, Addgene ID: 52962) and selected with 10μg/ml blasticidin (InvivoGen ...
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bioRxiv - Molecular Biology 2023Quote: ... The type I-F Vibrio cholerae HE-45 CAST was sub-cloned from Addgene (#130637 and #130633) [14] ...
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bioRxiv - Developmental Biology 2023Quote: ... and RYBP wild-type and mutant cDNAs using Gibson assembly into pdCas9-DNMT3A-2A-PuroR (Addgene# 71667)72 ...
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bioRxiv - Cell Biology 2024Quote: Lentiviral-TOP/FOP-dGFP-reporters (wild-type consensus plasmid TOP: Addgene plasmid #14715; http://n2t.net/addgene:14715; RRID:Addgene_14715; inverted consensus plasmid FOP: Addgene plasmid # 14885 ...
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bioRxiv - Cancer Biology 2021Quote: ... wild type or mutant FSCN1 (S39E and 149-151A mutants) cDNAs were subcloned into pLenti.CMV.blasticidin vector (Addgene, 17486) (Campeau et al. ...
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bioRxiv - Cell Biology 2020Quote: ... of L-type Ca2+ channels was a gift from Diane Lipscombe (Addgene plasmid # 26572; http://n2t.net/addgene:26572; RRID:Addgene_26572). The mApple-myosin X was subcloned by the Protein Cloning and Expression Core facility of the MBI.
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bioRxiv - Neuroscience 2022Quote: The pCMV-hEAAT2 plasmid encoding the human excitatory amino acid transporter type 2 (EAAT2) was a gift from Susan Amara (Addgene plasmid # 32814; http://n2t.net/addgene:32814; RRID:Addgene_32814). The cDNA was subcloned into pcDNA3 between KpnI and XbaI restriction sites and under the T7 promoter for expression in Xenopus laevis oocytes ...
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bioRxiv - Cancer Biology 2019Quote: ... The X22B and X22C guides were each cloned into the pX459 plasmid (with wild type Cas9; Addgene #48139) and the pX462 plasmid (with the D10A mutant version of Cas9 ...
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bioRxiv - Cancer Biology 2020Quote: ... The full-length wild-type cDNA of human BRCA2 was subcloned from pcDNA3 236HSC WT (Addgene plasmid # 16246) into the piggyBac vector ...
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bioRxiv - Neuroscience 2020Quote: ... we injected rAAV.mDLX.GCaMP6f24 (gift of Jordane Dimidschstein and Gordon Fishell) into wild type mice or injected AAV1.Syn.Flex.GCaMP6f (UPenn Vector Core or Addgene) into Gad2-Ires-Cre C57BL/6J mice (Jackson Laboratory) ...
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bioRxiv - Biochemistry 2020Quote: ... cDNA encoding wild-type human ubiquitin containing an N-terminal HA-tag was expressed from pRK5-HA (Addgene). Full-length EGFP fused N-terminally to a nuclear localization signal (NLS ...
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bioRxiv - Cancer Biology 2022Quote: ... human codon-optimized Streptococcus pyogenes wild-type Cas9 (Cas9-2A-GFP) was obtained from Addgene (Cat. No. 44719). Two chimeric guide RNA expression cassettes containing two of the following sgRNAs (sgRNA1 ...
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bioRxiv - Biochemistry 2022Quote: ... The plasmid expressing FLAG-tagged wild-type human HDAC1 was a gift from Eric Verdin (Addgene Plasmid # 13820). HCT116 cells were transfected in 10 cm plates with 8 μg plasmid and 40 μl PEI ...
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bioRxiv - Neuroscience 2022Quote: ... The wild type aequorin obtained by introducing two reverse mutations into the plasmid [pcDNA3.1+/mit-2mutAEQ] (Addgene #45539) (de la Fuente et al. ...