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Citations for Addgene :
851 - 900 of 1985 citations for Cis Dcca 100 Ug Ml In Acetonitrile D3 1 Carboxyl 13C2 99%; 1 D 97% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... and ESRG (6 μg each) along with 3 μg of pMD2.G (gift from Dr. D. Trono; RRID:Addgene_12259) was transfected into PLAT-GP packaging cells ...
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bioRxiv - Neuroscience 2021Quote: ... 2016) (Fig. 5A, Suppl. Figs. 3C,D and 8; Salk Vector Core; 1.0 x 1012; Addgene plasmid #55636); AAV1-SynP-DIO-splitTVA-EGFP-B19G (Fig ...
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bioRxiv - Microbiology 2023Quote: The construct for GRA47 complementation was created using the pUPRT::DHFR-D plasmid backbone from Addgene (Cat#58528). The DHFR cassette was eliminated through PCR amplification using primers pUPRT::GOI GIB F and pUPRT::GOI GIB R (Table S1) ...
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bioRxiv - Neuroscience 2022Quote: ... retrograde (1E12 GC/mL, Addgene 55637 ...
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bioRxiv - Neuroscience 2023Quote: ... Janelia) or AAVretro_Syn_GCamp7f (1.0e13 GC/ml, lot #21720; 1.2e12GC/ml, lot #v52598, Addgene 104488-AAVrg) in the right dorsolateral striatum (DLS ...
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bioRxiv - Cell Biology 2020Quote: 100 ng of human sgRNA library Brunello in lentiCRISPRv2 (Addgene, #73179) was transformed into electrocompetent Endura cells (#60242 ...
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bioRxiv - Neuroscience 2021Quote: ... 100-200 nl of AAV8-hSyn-DIO-hM4D(Gi)-mCherry (Addgene) were injected (DV −3.6 mm ...
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bioRxiv - Neuroscience 2023Quote: ... we injected 100 nl of AVV1-syn-FLEX-jGCaMP7f-WPRE (Addgene, catalog # 104492-AAV1 ...
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bioRxiv - Neuroscience 2023Quote: ... we injected 3×100 nl AAV5.Ef1a.DIO.eYFP (Penn Core, Addgene: 27056) into the mPFC (AP/ML/DV coordinates 2.2 ...
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bioRxiv - Cancer Biology 2021Quote: The PPARGC1A (PGC1α) gene was amplified from the vector pcDNA myc PGC-1 alpha (Addgene, 10974) using primers containing BamHI and EcoRI cloning sites (PPARGC1A_BamHI_F ...
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bioRxiv - Cell Biology 2020Quote: ... pGEX6P1-human LIC1 G domain (GST-LIC 1-389) was a gift from Ron Vale (Addgene plasmid # 74598 ...
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bioRxiv - Neuroscience 2021Quote: ... Constructs injected included: AAV2/1-CAG-FLEx-jGCaMP8 constructs (pGP-AAV-CAG-FLEx-jGCaMP8f-WPRE, Addgene plasmid #162382 ...
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bioRxiv - Molecular Biology 2020Quote: ... shRNA targeting the C/EBPα and ELF1 were cloned into pLKO.1 vector (Addgene, plasmid # 26655) harboring a blasticidin-selectable marker ...
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bioRxiv - Genomics 2020Quote: ... the whole cDNA was subcloned into the lentiviral CMV GFP destination vector 736-1 (Addgene #19732), and further used along with packaging vectors to generate lentiviral particles (control lentiviral particles were generated in the same manner using empty lentiviral vectors) ...
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bioRxiv - Cancer Biology 2019Quote: ... annealed oligos (Supplemental Table 1) were inserted into pSpCas9(BB)–2A–Puro (PX459) V2.0 (Addgene, #62988) using the BbsI (New England Biolabs ...
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bioRxiv - Immunology 2019Quote: ... Lentiviral vector pBABE-puro-SDF-1 alpha was a gift from Bob Weinberg (Addgene plasmid #12270) 75 ...
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bioRxiv - Neuroscience 2020Quote: ... the target sequence (5’-GGGTGAAGATCCTGTTCAATA-3’) was shuttled to the pLKO.1-Hygromycin vector (Addgene, #24150). For human astrocytes ...
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bioRxiv - Bioengineering 2019Quote: ... of a custom GFPACG gBlock cassette (Supplementary Table 1) into AcsI/AgeI-digested SGEN (Addgene #111171) backbone ...
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bioRxiv - Cell Biology 2021Quote: ... pclbw-opa1(isoform 1)-myc (myc-Opa1) was a gift from David Chan (Addgene plasmid # 62845) 47 ...
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bioRxiv - Cell Biology 2020Quote: ... HEK293T cells were transfected with the pLenti-PGK-Tacc3 or pLKO.1 TRC (control, Addgene 10879), pMD2G (Addgene 12259 ...
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bioRxiv - Biophysics 2020Quote: The DNA of the human kinesin-1 variant devoid of cysteine residue-encoding codons (Addgene #24430) consisted of amino acids 1 to 560 of KIF5B encoding a C-terminal 6xHis-tag[41] ...
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bioRxiv - Neuroscience 2022Quote: ... 2 µL of the adeno-associated viral vector rAAV9/1.EF1a.DIO.hChR2(H134R)-eYFP.WPRE.hGH (Addgene, #20298-AAV9) was dyed with 0.3 µL fast green (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2022Quote: ... Virus (0.25-1 μl) containing either AAV5: hSyn-DIO-hM3Dq-mCherry (excitatory DREADD; Addgene, Watertown MA), AAV5 ...
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bioRxiv - Molecular Biology 2020Quote: ... lentivirus destination vector or into the pLenti CMV Puro DEST (w118-1) vector (Addgene Plasmid #17452) using Gateway LR Clonase (Thermo Fisher ...
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bioRxiv - Molecular Biology 2021Quote: ... RRL.sin.cPPT.SFFV/TMPRSS2(variant 1).IRES-neo.WPRE (MT130) was a gift from Caroline Goujon (Addgene plasmid # 145843). pGBW-m4137383 was a gift from Ginkgo Bioworks (Addgene plasmid #149541) ...
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bioRxiv - Cancer Biology 2020Quote: ... Annealed gRNA oligos were ligated to pLKO.1-puro U6 sgRNA BfuAI stuffer (Addgene plasmid # 50920) or to pLenti SpBsmBI sgRNA Hygro (Addgene plasmid # 62205) ...
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bioRxiv - Genomics 2022Quote: ... 1-10 µg YAC/BAC payload DNA and 2-5 µg pCAG-iCre plasmid (Addgene #89573) per transfection ...
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bioRxiv - Molecular Biology 2020Quote: 1×106 cells of WTC p42 were transfected with 5mg AAVS1-TALEN R plasmid (Addgene #59026), 5 μg AAVS1-TALEN L plasmid (Addgene #59025) ...
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bioRxiv - Genetics 2020Quote: ... digested pENTR-LUC (w158-1; a kind gift from Eric Campeau & Paul Kaufman; Addgene plasmid #17473)(33 ...
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bioRxiv - Molecular Biology 2020Quote: ... The pLenti CMV rtTA3 Blast (w756-1) was a gift from Eric Campeau (Addgene plasmid #26429).
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bioRxiv - Cancer Biology 2020Quote: ... was cloned into pINDUCER20 (Meerbrey et al., 2011) and pLenti CMV Blast DEST (706-1) (Addgene plasmid #17451 was a gift from Eric Campeau & Paul Kaufman ...
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bioRxiv - Neuroscience 2019Quote: ... an AAV1:CBA:FLEX:Arch-GFP vector was used (UPenn [Addgene], cat. no. AV-1-PV2432 [22222-AAV1]). In mice for the control groups (Figs ...
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bioRxiv - Biochemistry 2021Quote: ... and ASW (Uniprot Q9BVC5-1) were subcloned into the UC Berkeley MacroLab 438B (Addgene plasmid #55219), 438Rgfp (Addgene plasmid #55221) ...
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bioRxiv - Biochemistry 2021Quote: ... and ASW (Uniprot Q9BVC5-1) were subcloned into the UC Berkeley MacroLab 438A (Addgene plasmid #55218) plasmid along with sequences encoding N-terminal affinity/fluorescence tags ...
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bioRxiv - Microbiology 2020Quote: ... pLenti CMV Blast DEST (706-1) (a gift from Eric Campeau & Paul Kaufman (Addgene plasmid #17451)) constructs carrying a human Plxdc1-TwinStrep or Plxdc2-TwinStrep expression cassette (pLenti-CMV-Blast-Plxdc1-Strep/ pLenti-CMV-Blast-Plxdc2-Strep ...
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bioRxiv - Cancer Biology 2021Quote: ... 2012) were cloned into the pLKO.1-Puro vector (a gift from R. Weinberg; Addgene #8453) to generate pLKO.1-Cys-TD and pLKO.1-Scr-TD ...
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bioRxiv - Immunology 2021Quote: ... Guide 1 (TGTTGGGGAACATATGACAC) and Guide 2 (AAGGAAAAATTCAAACAAGG) sequences were cloned into lentiGuide-puro plasmid (Addgene, #52963), transformed in Stbl3 bacteria (Invitrogen ...
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bioRxiv - Immunology 2020Quote: ... Major changes to the reported protocol included: 1) Use of a 2nd generation psPAX2 (Addgene, #12260) lentivirus packaging system instead of the 3rd generation system used by the Bloom lab ...
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bioRxiv - Neuroscience 2022Quote: ... mice were infused with diluted (1:5) or undiluted virus pAAV5-CAMKIIa-(hChR2- H134R)-eYFP (Addgene-ChR2 titre ...
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bioRxiv - Cell Biology 2022Quote: ... The GFP-RIAM(1-666) construct was a gift from Chinten James Lim (Addgene plasmid 80028) (Lee et al. ...
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bioRxiv - Genetics 2022Quote: ... Transfection mix for each shRNA contained 8.75μg of shRNA targeting construct in pLKO.1 (Addgene#8453), 8.75μg psPAX2 (Addgene#12260 ...
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bioRxiv - Molecular Biology 2022Quote: ... was used per manufacturer’s instruction to add 1 µg of the mRFP-UtrCH plasmid (Addgene #26739) to both WT and PDKO T-REx-293 cells seeded in 6-well plates at ∼70% confluency ...
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bioRxiv - Cancer Biology 2022Quote: ... Annealed gRNA oligos were ligated to pLKO.1-puro U6 sgRNA BfuAI stuffer (Addgene plasmid #50920), and lentiviral particles were generated ...
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bioRxiv - Neuroscience 2023Quote: ... 600 nL (in AuCx) or 1 µL (in IC) of AAV1-hSyn-hChR2(H134R)-EYFP (Addgene, Item# 26973-AAV1 ...
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bioRxiv - Molecular Biology 2023Quote: ... as well as overhangs for assembly into the STARR-seq vector (Addgene #99296; Supplemental Table 1) according to the manufacturer’s instruction (10 to 11 cycles of amplification) ...
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bioRxiv - Neuroscience 2022Quote: ... Mice were injected with ∼2000 nl of a 1:6 ratio of AAV1-Syn-Cre (AAV.hSyn.Cre.WPRE.hGH, 105553-AAV1, Addgene) and AAV1-CAG-tdTomato (59462-AAV1 ...
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bioRxiv - Genetics 2023Quote: ... and the annealed sense and antisense shRNA oligonucleotides were cloned into pLKO.1-puro vector (Addgene) for knockdown of human KISS1 ...
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bioRxiv - Developmental Biology 2023Quote: H2B-EGFP mRNA was transcribed from plasmid #1 pCS-H2B-EGFP (Megason, 2009) (Addgene, Plasmid #53744). To construct pCS2-myrTagRFP-T (plasmid #2) ...
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bioRxiv - Genetics 2023Quote: pHarvester was generated using pCFD5-gRNA#1&2 and pnos-Cas9-nos plasmids (Addgene no: 62208). Briefly ...
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bioRxiv - Cell Biology 2023Quote: ... Both pie-1p and pie-1 3’UTR PCR products were amplified using pPK605 plasmid (Addgene) as the template.