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Citations for Addgene :
451 - 500 of 2209 citations for Puumala Virus Glycoprotein 2 Gc Human Heterodimeric Fc tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... Pups were injected bilaterally with 4 ul of AAV9-hSyn-NES-jRCaMP1b (2.5×10^13 gc/ml, Addgene). Mice also received an injection of AAV9-hSyn-GRABACh3.0 to express the genetically encoded cholinergic sensor GRABACh3.0 48 ...
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bioRxiv - Neuroscience 2022Quote: ... chemogenetic non-projection specific inhibition experiments AAV8-hSyn-hM4D(Gi)-mCherry (Addgene #50475; 4.8×1012 GC ml-1). Retrograde rabies tracing25 ...
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bioRxiv - Neuroscience 2022Quote: ... Retrograde tracing was performed using the viral vectors AAV2retro-CAG-FLEX-tdTomato (1.3E13 GC/ml) obtained from Addgene (provided by Edward Boyden ...
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bioRxiv - Neuroscience 2023Quote: ... Rats intended for optogenetic experiments received AAV9 DO-hChR2-mCherry (0.4uL, titer ∼ 3.1 × 1013 GC/m, Addgene # 20297) injected unilaterally into the VP and retro-AAV Cre (titer ∼ 1.1 × 1013 GC/mL ...
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bioRxiv - Biochemistry 2023Quote: ... mice were injected via the tail vein with 2.5×1011 GC of AAV-TBG-Cre (Addgene, 107787-AAV8) in PBS ...
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bioRxiv - Neuroscience 2022Quote: ... For in vivo imaging: Two 300 nl injections of AAV2retro-pkg-Cre (Addgene 24593, 1.4 × 1013 GC/ml) and AAV2retro-syn-jGCaMP7f-WPRE (Addgene 104488 ...
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bioRxiv - Neuroscience 2023Quote: ... 300nL of AAV/DJ-hSyn1-DIO-tdTomato-T2A-Syp::eGFP-WPRE (2.5 × 1013 GC/mL from Addgene #51509) was injected unilaterally at a rate of 60nL/sec ...
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bioRxiv - Neuroscience 2023Quote: ... Either AAV2/8-hSYN-JAWS-tdTomato-ER2 (Neurophotonics, diluted 2.5x from 9.8e2 GC/ml stock into PBS) or AAV8-hSyn-mCherry (Addgene catalog #114472 ...
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bioRxiv - Neuroscience 2023Quote: ... AAV8-pEF1a-DIO-FLPo-WPRE-hGHpA (2x1012 gc/ml) was a gift from Li Zhang (Addgene plasmid # 87306) (Zingg et al. ...
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bioRxiv - Neuroscience 2023Quote: ... AAV8-hSyn-DIO-hM3D(Gq)-mCherry (1.83x1012 gc/ml) was a gift from Bryan Roth (Addgene plasmid # 44361) (Krashes et al. ...
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bioRxiv - Neuroscience 2024Quote: ... University of Pennsylvania vector core) and AAV1-Syn-Flex-GCaMP6f-WPRE-SV40 (0.4 μl, 1.3 x 1013 GC/ml, Addgene) was injected into the IL ...
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bioRxiv - Cancer Biology 2023Quote: ... male mice were injected with 6.4 X 108 GC/mouse of AAV8.TBG.PI.Cre.rBG (AAV-TBG-Cre, Addgene, 107787-AAV8) diluted in 100μL PBS via the tail vein ...
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bioRxiv - Neuroscience 2021Quote: ... CalR-HaloTag-Snap-tag-KDEL (ER-localizing) or GCaMP6f (Addgene 100837-AAV1). On day 14-18 the neurons were incubated for at least 2 h with 1 μM of the corresponding MaPCa dyes containing 0.04% Pluronic-F127 ...
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bioRxiv - Biochemistry 2019Quote: ... coli Destination vector pDest-566 (N-terminal His6-MBP tag, Addgene #11517). Final baculovirus expression clones were transformed into DH10Bac cells (ThermoFisher) ...
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bioRxiv - Molecular Biology 2020Quote: ... The 6xHis-MBP tag was PCR amplified from pDZ2087 construct (Addgene, #92414) with primers MBP F and MBP R ...
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bioRxiv - Genomics 2023Quote: ... Mito-tag plasmids were based on pMXs-3XHA-EGFP-OMP25 (Addgene, #83356) and pMXs-3XMyc-EGFP-OMP25 (Addgene ...
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bioRxiv - Cell Biology 2024Quote: ... pMXs-3XHA-EGFP-OMP25 was obtained for MITO-Tag experiments (Addgene #83356). CRISPR/Cas9 plasmids for control and SLC25A48-KO (Santa Cruz Biotechnology ...
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bioRxiv - Neuroscience 2022Quote: The pCMV-hEAAT2 plasmid encoding the human excitatory amino acid transporter type 2 (EAAT2) was a gift from Susan Amara (Addgene plasmid # 32814 ...
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bioRxiv - Plant Biology 2020Quote: The human codon-optimized CAS9 with 2×35S CaMV promoter and Nos terminator was amplified from pAGM4723 plasmid (Addgene# 49772) using KpnI forward and PacI reverse primers (Supplemental Table 9) ...
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bioRxiv - Cancer Biology 2019Quote: ... The plasmid construct targeting exon 2 of human AIRE (AIREKO) was created using pSpCas9(BB)-2A-GFP (a gift from Dr. Feng Zhang, #48138, Addgene, http://n2t.net/addgene:48138 ...
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bioRxiv - Neuroscience 2023Quote: A 2.2 kb BamHI-SalI cDNA fragment of the long form of human PREPL (PREPLL) was cloned into pLenti-GFP (Addgene) digested with the same restriction enzymes ...
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bioRxiv - Cancer Biology 2023Quote: ... sgRNAs targeting mouse and human genes of interest (see Supplementary Table 2) were cloned into plenti-sgRNA (Addgene plasmid #71409) using BsmbI and into Cas9 plasmid (Addgene plasmid #62988)(Ran et al ...
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bioRxiv - Neuroscience 2021Quote: ... Mice were bilaterally microinjected with 500 nL of AAV1.Syn.GCaMP6f.WPRE.SV40 virus (purchased from Addgene 100837) at 20 -120 nL/min into the dorsal cortex using the stereotactic coordinates ...
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bioRxiv - Bioengineering 2021Quote: ... Lentivirus pseudotyped with the vesicular stomatitis virus spike G (pCMV-VSV-G; Addgene plasmid # 8454) was used as a positive control and the bald lentivirus having no coat was used as a negative control.
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bioRxiv - Neuroscience 2021Quote: MSCV (Murine Stem Cell Virus)-CreERT2 puro plasmid was a gift from Tyler Jacks (Addgene, Watertown ...
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bioRxiv - Cancer Biology 2020Quote: HT29 cells were infected with the pLenti-U6-tdTomato-P2A-BlasR (LRT2B) virus (Addgene, 1108545) that enables dual production of firefly luciferase and dTomato ...
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bioRxiv - Neuroscience 2021Quote: ... DV 1.4) before injecting 250 nL of virus (pGP-AAV1-syn-jGCaMP7f-SV40-WPRE; Addgene) in 50 nL pulses each interspersed by ∼10 s and the final pulse followed by a 10 min period where the needle was left in place to allow for diffusion ...
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bioRxiv - Neuroscience 2020Quote: ... We then injected the AAV5.CamKII.GCaMP6f.WPRE.SV40 virus (Addgene # 100834; 200 nL at 1 nl.s-1) in hippocampal CA1 using the following coordinates ...
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bioRxiv - Physiology 2020Quote: ... A retrograde AAV virus carrying a Cre gene (AAVrg-hSyn1-Cre, Addgene; 200 nl volume) was injected into the PBN (coordinates ...
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bioRxiv - Cancer Biology 2022Quote: MB-004 cells were transduced with the enti-dCas9-KRAB-T2A-GFP virus (Addgene # 71237). sgRNAs targeting SOX11/HNRNPH1-binding motifs in the distal enhancers were designed using the CHOPCHOP program (https://chopchop.cbu.uib.no) ...
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bioRxiv - Neuroscience 2022Quote: ... and 0.5µl of virus (either AAV1-CaMKIIa-ArchT-GFP or AAV8-CaMKIIa-GFP, Addgene, MA) was infused bilaterally with a 1 µl Hamilton syringe into either the PRC (AP ...
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bioRxiv - Neuroscience 2022Quote: ... Anxa1-iCre mice (new line) were injected with AAV1-CAG-FLEX-GCaMP6f virus (RRID: Addgene_100835). For labelling VGlut2+ or Aldh1a1+ dopamine neurons ...
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bioRxiv - Neuroscience 2023Quote: ... the pipette containing AAVPHP.S-CAG-FLEX-tdTomato virus (Addgene 28306-PHP.S, 1.8×1013 vg/mL) targeted at 2 locations in the mandibular branch spaced 250 um apart ...
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bioRxiv - Neuroscience 2023Quote: ... virus expressing tdTomato under the PVIN specific S5E2 enhancer (AAV9-S5E2-tdTomato, Addgene 135630-AAV9) was injected in mice using coordinates ...
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bioRxiv - Neuroscience 2023Quote: Unilateral stereotaxic injections of AAV5-CaMKIIα-EGFP (Addgene #50469; virus titer ≥ 3×10¹² vg/mL) and AAV5-hSyn-DIO-hM4D(Gi)-mCherry (Addgene #44362 ...
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bioRxiv - Neuroscience 2023Quote: ... and AAV5-hSyn-DIO-hM4D(Gi)-mCherry (Addgene #44362; virus titer ≥ 7×10¹² vg/mL) viruses were performed on adult anesthetized (isoflurane ...
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bioRxiv - Neuroscience 2021Quote: ... and retrograde AAV encoding Cre recombinase (AAVretro-hSyn-HI-eGFP-Cre (titer 1.17 x 1013 GC/ml, Addgene 105540) were stereotaxically injected into S2 or M1 and nwS1 ...
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Basolateral amygdala parvalbumin neurons report aversive prediction error to constrain fear learningbioRxiv - Neuroscience 2020Quote: ... AAV9-Syn-FLEX-gCaMP7s (1.2×1013 GC/ml) (pGP-AAV-syn-FLEX-jGCaMP7s-WPRE was a gift from Douglas Kim & GENIE Project (Addgene viral prep # 104491-AAV9 ...
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Basolateral amygdala parvalbumin neurons report aversive prediction error to constrain fear learningbioRxiv - Neuroscience 2020Quote: ... AAV5-ef1-DIO-eYFP (3.3×1012 GC/ml) (pAAV-Ef1a-DIO EYFP was a gift from Karl Deisseroth (Addgene viral prep # 27056-AAV5 ...
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bioRxiv - Neuroscience 2020Quote: ... bilaterally injected using a pulled glass needle in the hippocampal area with 0.5 μL AAV-hSyn-Cre-EGFP at 3 × 1012 GC ml-1 (Addgene #105540 ...
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bioRxiv - Neuroscience 2021Quote: ... FL: 24.5 μl of AAV9-hSyn-ChR2-eYFP (UPenn Lot: CS0964 based on Addgene 26973P, titer: 1.03e13 GC/ml). The construct was loaded into a 10 μl Nanofil syringe (World Precision Instruments ...
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bioRxiv - Molecular Biology 2021Quote: AAV8-hSyn-DIO-hM3D(Gq)-mCherry (titer:4.8×1012 GC/mL) (pAAV-hSyn-DIO-hM3D(Gq)- mCherry was a gift from Bryan Roth (Addgene viral prep #44361-AAV8 ...
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bioRxiv - Neuroscience 2020Quote: ... we also injected 100 nL anterograde viral tracer (AAV1-CB7-CI-TurboRFP-WPRE-RBG, 2.2 × 1012 gc/mL, catalog # 105546-AAV1, Addgene) into the ventral posteromedial parvocellularis (VPMpc ...
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bioRxiv - Neuroscience 2020Quote: ... Chronos was manufactured at the University of Pennsylvania Vector Core (AAV2/8.Syn.Chronos.tdTomato, Addgene 62726, 1.6×1013 GC/ml). oChIEF was manufactured by Virovek (AAV2/1.CAG.flex.oChIEF.tdTomato ...
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bioRxiv - Neuroscience 2021Quote: ... 6-OHDA injection was immediately followed by unilateral injection of AAV8-hSyn-DIO-hM4D(Gi)-mCherry (2.9×1013 GC/mL, Addgene) into the striatum ipsilateral to the lesioned side of ChATcre/wt mice ...
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bioRxiv - Neuroscience 2022Quote: ... we injected either rAAV2/9 encoding GCaMP6s (Chen et al., 2013) under control of the CaMKII promoter (1.25 × 1013 gc/ml; AddGene viral prep #107790-AAV9 ...
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bioRxiv - Neuroscience 2022Quote: ... and 210nl of Arch 3.0- eYFP (rAAV2-EF1a-double floxed-eArch3.0-eYFP; 5 × 1011 GC per ml; UNC Vector Core or eYFP (Addgene plasmid 20296 ...
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bioRxiv - Neuroscience 2021Quote: ... heterozygous D1-Cre or D2-Cre mice were unilaterally injected with 1200 nl of AAV9-FLEX-jGCaMP7f (9.6×1012 GC/ml, Addgene) were stereotaxically injected using a Nanoject III instrument (Drummond ...
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bioRxiv - Neuroscience 2022Quote: ... 0.8 uL of a 1:1 mixture of EGFP (AAV9- Hsyn-DIO-EGFP;4.6*10^13 GC/mL; Addgene) and AAV8-GAD1-cre was injected unilaterally into VP ...
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bioRxiv - Neuroscience 2022Quote: ... or rAAV2/9 encoding for NIR-GECO2 under control of the synthetic CAG promoter (0.5 × 1012 -1 × 1013 gc/ml; AddGene plasmid #159603 ...