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No products found
because this supplier's products are not listed.
Ugo Tomasello, et al.,
bioRxiv - Evolutionary Biology 2021
Quote:
... three sections for each brain electroporated at E14.5 with pUB6-TOM and pSilencer-U6-scram (number of brains, n = 4) or pSilencer-U6-miR-137 (number of brains, n = 4) and injected with retrobeads (Lumafluor) at P9 ...
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No products found
because this supplier's products are not listed.
Jiling Feng, Yuexun Tang, Wenwei Fu, Hongxi Xu,
bioRxiv - Cell Biology 2023
Quote:
... RT-PCR was performed with a one-step real time PCR using KAPA SYBR FAST One-Step qRT-PCR Universal (D-MARK Biosciences). Primers were used as previously described [18] ...
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TTP22 is a casein kinase 2 (CK2) inhibitor with an IC50 value of 100 nM. It displays selectivity...
Cat# S6536, SKU# S6536-5mg,
5mg, $97.00
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Emiliano Hergenreder, et al.,
bioRxiv - Neuroscience 2022
Quote:
... 22 confirmed compounds were purchased from Selleck Chemicals, reconstituted in a suitable solvent and applied for dose-response validation in a concentration log scale (30nM ...
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No products found
because this supplier's products are not listed.
Maxime Mivelaz, et al.,
bioRxiv - Biophysics 2019
Quote:
... instrument calibration was performed by imaging 100-nm biotinylated Gold nanoparticles (Cytodiagnostics) with 532 nm excitation and 100 ms time-resolution over 10 s ...
-
No products found
because this supplier's products are not listed.
Ying Yang, et al.,
bioRxiv - Cell Biology 2023
Quote:
... where the working electrode was the indium tin oxide (ITO) coated coverslips (8-12 Ω, 22×22 cm, SPI Supplies, USA).
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PCR Mycoplasma Detection Kit is designed to detect the presence of mycoplasma contamination by...
Cat# abx298017-100RXNS,
100 rxns USD $507.5
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Xinmeng Li, et al.,
bioRxiv - Neuroscience 2023
Quote:
... the supernatants were collected for DBI detection using DBI ELISA kit from Abbexa Ltd (abx153899) ...
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No products found
because this supplier's products are not listed.
Galen J. Correy, et al.,
bioRxiv - Biochemistry 2022
Quote:
... MicroRT tubing (Mitegen, RT-T1) with 5 μl of reservoir in the end was placed over the crystals to prevent dehydration ...
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No products found
because this supplier's products are not listed.
Sara M. Blazejewski, et al.,
bioRxiv - Neuroscience 2020
Quote:
pCAG-eCas9-GFP-U6-Rpsa-gRNA plasmid was transfected into mouse Neuro-2a cells using PolyJet transfection reagent (SignaGen Laboratories), and Genomic DNA was isolated and subjected to PCR to amplify the 433 bp fragment containing gRNA target sequence using Q5 High Fidelity DNA polymerase (NEB ...
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No products found
because this supplier's products are not listed.
Alexander D. Coleman, et al.,
bioRxiv - Plant Biology 2019
Quote:
... The soluble fraction was dialyzed in ⌀22 mm tubing with a 14 kDa cut-off (Biomol, Hamburg, Germany) overnight at 4°C and subsequently lyophilized ...
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No products found
because this supplier's products are not listed.
Wei-Wen Lim, et al.,
bioRxiv - Pathology 2019
Quote:
The amount of total tissue collagen was quantified using colorimetric detection of hydroxyproline using the Quickzyme Total Collagen assay kit (Quickzyme Biosciences) performed according to the manufacturer’s protocol ...
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No products found
because this supplier's products are not listed.
Xiaoxin X. Wang, et al.,
bioRxiv - Pathology 2020
Quote:
... UnoVue HRP secondary antibody detection reagent (Diagnostic BioSystems, Pleasanton, CA) was applied followed by DAB chromogen ...
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No products found
because this supplier's products are not listed.
Román Ramos Báez, et al.,
bioRxiv - Plant Biology 2019
Quote:
... and the products were purified using the EZNA Cycle Pure® Kit or the NEB Monarch® PCR & DNA Cleanup Kit and confirmed by sequencing (MCLab or Genewiz).
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No products found
because this supplier's products are not listed.
Kristina Bartmann, et al.,
bioRxiv - Pharmacology and Toxicology 2023
Quote:
... washed three times with PBS (PAN Biotech), and then incubated overnight at 4°C with primary antibodies diluted in blocking solution [5% Goat Serum (Sigma Aldrich ...
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No products found
because this supplier's products are not listed.
Anne-Perrine Foray, et al.,
bioRxiv - Immunology 2021
Quote:
... and an anti-IFNγ capture and detection antibody pair (U-CyTech Biosciences) were used ...
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No products found
because this supplier's products are not listed.
Sangsu Bang, et al.,
bioRxiv - Neuroscience 2023
Quote:
... followed by detection using an anti-GRP37L1 antibody (Bioss, Rabbit, 1:1000, Cat No. bs-15390R) or anti-flag antibody (Cell signaling ...
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No products found
because this supplier's products are not listed.
Sarah Bahraoui, et al.,
bioRxiv - Cell Biology 2023
Quote:
... Quantitative PCR was performed on 6,25ng of cDNA using the SensiFAST™ SYBR® No-ROX kit (Bioline, Meridian Life Science© Company) and a LightCycler® 480 Detection system (Roche) ...
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No products found
because this supplier's products are not listed.
Nils Burger, et al.,
bioRxiv - Cell Biology 2024
Quote:
... Membranes were washed three times with TBS + 0.1% Tween (Boston BioProducts, USA) and membranes were scanned using an Odyssey DLx (LI-COR Biosciences ...
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No products found
because this supplier's products are not listed.
Seesandra V. Rajagopala, et al.,
bioRxiv - Genomics 2022
Quote:
... and 7.25 μl PCR Certified Water (Teknova). RNA was reverse transcribed at 50ºC for 15 minutes ...
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No products found
because this supplier's products are not listed.
Marie-Laure Fogeron, et al.,
bioRxiv - Biochemistry 2021
Quote:
... Protein detection on the blots was performed with an antibody against the Strep-tag II (StrepMAB-Classic, IBA Lifesciences, Germany).
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No products found
because this supplier's products are not listed.
Ahmed Ali, et al.,
bioRxiv - Biophysics 2023
Quote:
Mouse sera were diluted ten times in PEG dilution buffer (PEGD50-1, Life Diagnostics, Inc.), and sequentially in 1:3 dilution steps added to the wells of PEG-BSA coated ELISA plates (PBSA20PL ...
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No products found
because this supplier's products are not listed.
Jingjing Lou, et al.,
bioRxiv - Microbiology 2023
Quote:
... mAID CDS PCR amplified (TruFi™ DNA Polymerase; Azura Genomics) from plasmid plinker-AID-Ty-HXGPRT-LoxP using primers P8 and P9 ...
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No products found
because this supplier's products are not listed.
Suchitra Joshi, et al.,
bioRxiv - Neuroscience 2023
Quote:
... The estradiol levels (n=4) were also measured using an ELISA assay (#ES180S-100 Calbiotech, detection range 3 to 300 pg/mL). The intra-assay variation was 5.9% in these assays.
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No products found
because this supplier's products are not listed.
Glory Nasseri, et al.,
bioRxiv - Neuroscience 2021
Quote:
... resuspended proteins were PEGylated for 1 hr at RT to label newly exposed cysteine thiols with the 5 kDa mass tag reagent (mPEG-5k, Badrilla SiteCounter™). Approximately 20 μl of each supernatant was saved as the “total input.” For immunoblot analysis ...
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No products found
because this supplier's products are not listed.
M. Koltun, et al.,
bioRxiv - Neuroscience 2019
Quote:
... Each time tissues were homogenized using Bullet Blender with 0.5 mm RNase Free Zirconium Oxide Beads (Next Advance) in RLT buffer from RNeasy Plus Micro Kit (Qiagen) ...
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No products found
because this supplier's products are not listed.
Eric T. Son, et al.,
bioRxiv - Immunology 2021
Quote:
... QuickSwitch Custom Tetramer Kits (MBL International) were utilised to generate multiple tetramers with selected peptides in order to screen an array of pMHC epitopes ...
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No products found
because this supplier's products are not listed.
Stavros Giaglis, et al.,
bioRxiv - Immunology 2021
Quote:
... utilizing the human TAT Complexes ELISA Kit (Assaypro) and the Imuclone D-Dimer ELISA Kit (American Diagnostica ...
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No products found
because this supplier's products are not listed.
Ruud H. Wijdeven, et al.,
bioRxiv - Immunology 2021
Quote:
... rabbit anti-TEAD1 (ProSci 22-472), mouse anti-TAZ (Santa Cruz ...
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No products found
because this supplier's products are not listed.
Zhipeng Li, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... Solution standard: 2 µl of calibration standard #2 (“CEM2”, LGC Standards #VHG-SM70B-100) was added to 200 µl of 50% HNO3 and heated for 2 hrs at 90°C in a loosely capped 15ml centrifuge tube (VWR ...
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No products found
because this supplier's products are not listed.
Jinze Li, et al.,
bioRxiv - Neuroscience 2023
Quote:
Blood flow in the mouse brain was recorded in real-time using an RFLSI III device (RWD Life Sciences, Shenzhen, China) throughout the whole process of ischemic stroke ...
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No products found
because this supplier's products are not listed.
S. Marsit, et al.,
bioRxiv - Evolutionary Biology 2020
Quote:
... (2019)22 with a SporePlay™ dissection microscope (Singer Instruments, Somerset, UK) on YPD plates and incubated for 5 days at 25 °C ...
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No products found
because this supplier's products are not listed.
Yasmin Fareed, et al.,
bioRxiv - Pharmacology and Toxicology 2022
Quote:
Pooled biofluid samples for matrix-matched calibration were obtained as follows: lithium-heparin plasma was purchased from Innovative Research (Novi, Michigan). Pooled urine was provided by an Austrian female volunteer who avoided the consumption of food and use of cosmetics stored in plastic containers ...
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No products found
because this supplier's products are not listed.
Priyasha Deshpande, et al.,
bioRxiv - Biophysics 2024
Quote:
... Unmodified and methylated histone H3 peptides (1-22) (MW =2354) were purchased in lyophilized form from Bachem Americas,Inc (Torrance ...
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No products found
because this supplier's products are not listed.
Marina Schrecker, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... 3.5 μL of purified protein at a concentration of 0.22 mg/mL was applied to Graphene Oxide Au 400 mesh QUANTIFOIL R1.2/1.3 holey carbon grids (Quantifoil), and then plunged into liquid nitrogen-cooled liquid ethane with a FEI Vitrobot Mark IV (FEI Thermo Fisher) ...
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No products found
because this supplier's products are not listed.
Joshua B. R. White, et al.,
bioRxiv - Microbiology 2022
Quote:
... The cells were lysed with a single pass at 22 kpsi through a cell disruptor (0.75 kW; Constant Systems). Membranes were isolated by ultracentrifugation for 45 min at 42,000 rpm (45 Ti rotor ...
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No products found
because this supplier's products are not listed.
Brian W. Ji, et al.,
bioRxiv - Microbiology 2019
Quote:
... inverted sterile 200 µL pipette tips (Rainin RT-L200F) were used to core out a small sample from the stool ...
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No products found
because this supplier's products are not listed.
Pradeep Ramalingam, et al.,
bioRxiv - Cell Biology 2020
Quote:
... Established BMEC lines were transduced with GFP-tagged RNAi lentivirus (BLOCK-iT HiPerform Lentiviral Pol II miR RNAi Expression system, Invitrogen Cat# K4934-00) targeting Raptor or Rictor ...
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No products found
because this supplier's products are not listed.
Brandon M Bensel, et al.,
bioRxiv - Biophysics 2023
Quote:
... Liposomes were then extruded to their final size of 350 nm diameter (22) through a 200 nm pore diameter extruder (T&T Scientific).
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No products found
because this supplier's products are not listed.
Maximilian Bunz, et al.,
bioRxiv - Microbiology 2023
Quote:
... or SiR-DNA dye (1 µM, 90 min, RT; Tebu-Bio). Three final washing steps were performed ...
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No products found
because this supplier's products are not listed.
Mikolaj J. Filon, et al.,
bioRxiv - Neuroscience 2020
Quote:
... Mice were dosed once daily with fenobam and once every other day with CTEP at 400 μL per 40g body weight by oral gavage with 22g 1.4” feeding needles with ball (Kent Scientific, catalog #FNC-22-1.5). Final drug concentrations were 24 mg/kg fenobam and 2 mg/kg CTEP ...
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No products found
because this supplier's products are not listed.
C Bourges, et al.,
bioRxiv - Genetics 2020
Quote:
Oligonucleotide libraries were re-suspended in nuclease-free water and amplified by emulsion PCR (NEB Q5 polymerase, 30 cycles, Micellula DNA Emulsion & Purification Kit (Chimerx)) using primers containing SfiI restriction sites ...
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No products found
because this supplier's products are not listed.
Sanne van der Niet, et al.,
bioRxiv - Cell Biology 2020
Quote:
... Dihydrochloride) detection was performed using anti dinitrophenol (DNP) (Polyclonal Anti-DNP, Oxford Biomedical Research). All antibodies were diluted in 1% BSA in PBS ...
-
No products found
because this supplier's products are not listed.
Dena Ennis, et al.,
bioRxiv - Microbiology 2023
Quote:
... Samples were separated by HPLC with fluorescence detection on a TSKgel Amide-80 column (Tosoh Bioscience, Tokyo, Japan) with a linear gradient of a 50 mM ammonium formate/acetonitrile solvent system ...
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No products found
because this supplier's products are not listed.
Jingyao Li, et al.,
bioRxiv - Genomics 2021
Quote:
... 10 nl of 1 uM barcoded DRUG-seq RT primers were then dispensed into each well using an Echo 555 Liquid Handler (Labcyte). Plates were sealed (BioRad ...
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No products found
because this supplier's products are not listed.
Clara Sidor, et al.,
bioRxiv - Developmental Biology 2019
Quote:
... was amplified by PCR from the mNeonGreen vector (Allele Biotechnology), with the exclusion of the stop codon and the addition of a C-terminal linker.
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No products found
because this supplier's products are not listed.
Cody Juguilon, et al.,
bioRxiv - Physiology 2021
Quote:
... The beads with EC were washed several times and cultured in mouse EC cultured medium (Cell Biologics). When the cells were confluent ...
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No products found
because this supplier's products are not listed.
Mindy Engevik, et al.,
bioRxiv - Cell Biology 2022
Quote:
Albumin concentration in stool was determined using a commercial kit (Albumin ELISA Kit, Genway, GWB-282C17) according to the manufacturer’s instructions ...
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No products found
because this supplier's products are not listed.
Wyatt G. Paltzer, et al.,
bioRxiv - Developmental Biology 2023
Quote:
... Aniline Blue Stain Kit” (Newcomer Supply, #9179B) following the kit protocol ...
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No products found
because this supplier's products are not listed.
Ichiro Yamauchi, et al.,
bioRxiv - Physiology 2023
Quote:
... TSH AccuLite VAST CLIA kit (Monobind Inc., Lake Forest, CA). We diluted serum samples 25-fold with phosphate buffered saline when we measured human TSH levels ...
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No products found
because this supplier's products are not listed.
JM Robinson, et al.,
bioRxiv - Immunology 2019
Quote:
... I-FABP (Human I-FABP ELISA Kit, Hycult biotech, Cat# HK406), and LBP (Human Lipopolysaccharide Binding Protein ELISA Kit ...
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No products found
because this supplier's products are not listed.
Caroline Passaes, et al.,
bioRxiv - Immunology 2019
Quote:
... Culture supernatants were assayed on day 7 using an SIV p27 Antigen ELISA Kit (Zeptometrix). Antiviral activity was calculated as log10 (mean p27 ng/mL in SIV-infected CD4+ T-cell cultures without CD8+ T-cells ...