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No products found
because this supplier's products are not listed.
Francisco J. Calero-Cuenca, et al.,
bioRxiv - Cell Biology 2020
Quote:
... Reverse transcription quantitative PCR (RT-qPCR) was performed using Power SYBR Green PCR MasterMix (Alfagene) according to the manufacturer’s instructions and using primers forward and reverse at 0,25 μM (final concentration ...
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No products found
because this supplier's products are not listed.
Sandra L. Bixler, et al.,
bioRxiv - Microbiology 2021
Quote:
Total SARS-CoV-2 E gene and subgenomic E gene target copy numbers were determined by real-time RT-PCR using previously described assays (24–25) and a synthetic RNA containing the subgenomic E RNA sequence (Bio-Synthesis, Inc., Lewisville, TX, USA). Extracted nucleic acid was tested in triplicate (5 μL extracted nucleic acid ...
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No products found
because this supplier's products are not listed.
Enrique Lin-Shiao, et al.,
bioRxiv - Bioengineering 2021
Quote:
... and 10 μg U6-ELS77 plasmids using polyethylenimine (Polyscience Inc.). Transfected cells were switched into Optimem (Gibco ...
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No products found
because this supplier's products are not listed.
Lucia Zubizarreta, et al.,
bioRxiv - Neuroscience 2023
Quote:
Calibration curves were made from certified reference standards (Cerilliant Co., Round Rock, TX) prepared in 50% HPLC-grade methanol ...
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No products found
because this supplier's products are not listed.
Chiu-An Lo, Brian Chen,
bioRxiv - Cell Biology 2019
Quote:
The optimal sgRNA in pCFD3: U6:3-gRNA vector was microinjected into embryos (BestGene, Inc) to create a transgenic fly constitutively expressing the Rpl13a-specific sgRNA ...
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No products found
because this supplier's products are not listed.
Hiroto Yamashita, et al.,
bioRxiv - Plant Biology 2023
Quote:
... Compounds were quantified using calibration curves for the following chemicals: chicoric acid and chlorogenic acid purchased from Toronto Research Chemicals (Toronto, Canada), quercetin-3-O-glucoside purchased from Extrasynthese (Genay ...
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No products found
because this supplier's products are not listed.
Ricardo M. Santos, Anton Sirota,
bioRxiv - Neuroscience 2020
Quote:
All in vitro tests were done in a stirred calibration buffer kept at 37 °C using a circulating water pump (Gaymar heating/cooling pump, Braintree Scientific, Inc., USA) connected to the calibration beaker ...
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No products found
because this supplier's products are not listed.
Salim Arslan, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... study [22] (i.e TCGA-BRCA) and 2) a proprietary dataset by a private clinical data provider (e.g. BioIVT). All samples were following the inclusion criteria described in Target Patient Groups.
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No products found
because this supplier's products are not listed.
Manuela Fuchs, et al.,
bioRxiv - Microbiology 2020
Quote:
... overnight at 22 °C followed with two bead purification steps with 2 volumes of MagSi-NGSPREP Plus beads (AMSBIO). A PCR enrichment test was performed in order to determine the number of PCR cycles that are nesessery for each pool followed by a bead purification step and a QC with DNA HS kit (Agilent) ...
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No products found
because this supplier's products are not listed.
Qiang Sun,
bioRxiv - Plant Biology 2021
Quote:
... Xylem segments were then silver-enhanced at RT for 15 min with a silver enhancement kit (BBI Solutions, UK, Batch No. 12348). After wash with DD H2O and dehydration via an ethanol series ...
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No products found
because this supplier's products are not listed.
Maximilian Peer, et al.,
bioRxiv - Biochemistry 2022
Quote:
... Initial crystallization screenings were carried out at 22°C by the sitting drop vapor diffusion method using the Phoenix HT liquid handling robot (Rigaku) to set up dual droplets for each condition with drop volume of 0.2 and 0.3 µl (1:1 and 2:1 ...
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No products found
because this supplier's products are not listed.
Jamal Green, et al.,
bioRxiv - Immunology 2024
Quote:
... Gavages were performed using a sterile syringe and a straight 1-inch-long 22-gauge gavage needle (Cadence Science, #7901). For post-weaning colonization experiments ...
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No products found
because this supplier's products are not listed.
Malin C. Erlandsson, et al.,
bioRxiv - Immunology 2021
Quote:
... IFNγ (PelikineM1933, detection limit 3pg/ml, Sanquin, Amsterdam, the Netherlands), IL10 (DY217B ...
-
No products found
because this supplier's products are not listed.
Marshall Lukacs, et al.,
bioRxiv - Genetics 2019
Quote:
... was used for detection on an Alliance chemiluminescence imaging system (UVITEC Cambridge).
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No products found
because this supplier's products are not listed.
Philipp Starkl, et al.,
bioRxiv - Immunology 2023
Quote:
... Primary antibodies and detection reagents used: polyclonal chicken anti-GFP (Aves labs); anti-beta-tubulin III (clone 2G7D4 ...
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No products found
because this supplier's products are not listed.
Afrida Rahman-Enyart, et al.,
bioRxiv - Systems Biology 2021
Quote:
... We allowed particulates to settle for 30 s before drawing supernatant into a syringe attached to a 22-gauge gavage needle (Fine Science Tools, Foster City, CA). We pooled together 500 μL of the supernatants from each of the three 1 mL suspensions and deposited 200 μL of this pooled supernatant into the stomach of each recipient mouse by gavage ...
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No products found
because this supplier's products are not listed.
Qi Wang, et al.,
bioRxiv - Genomics 2023
Quote:
... and RNasin Plus (Promega)] 10-15 times using pestle A “loose” followed by pestle B “tight” 10-15 times (DWK Life Sciences, Millville, NJ, USA). Homogenate was passed through a 70 µm 1.5 ml mini strainer (PluriSelect ...
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No products found
because this supplier's products are not listed.
Teresa R. Wagner, et al.,
bioRxiv - Immunology 2023
Quote:
... followed by adding the M13-HRP-labeled detection antibody (Progen, 1:2000 Dilution) for 1 h ...
-
No products found
because this supplier's products are not listed.
Thomas HB FitzGerald, et al.,
bioRxiv - Neuroscience 2019
Quote:
... agents update beliefs about all states within a fixed-length time window that includes the present time but stretches a set distance into the past (Figure 1) (FitzGerald et al., 2017). This window moves forward in time at the same rate that observations are gathered ...
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No products found
because this supplier's products are not listed.
Zhexin Wang, et al.,
bioRxiv - Cell Biology 2020
Quote:
... and 3 times at 10,000 rpm for 5 s (IKA TC10 basic ULTRA-TURRAX® homogenizer with S10N-5G dispersing element ...
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No products found
because this supplier's products are not listed.
Lizhi He, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... lysed by grinding 50 times in Wheaton A (Wheaton, 357538), and then incubated on ice for 20 minutes ...
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No products found
because this supplier's products are not listed.
Michelle E. Maxson, et al.,
bioRxiv - Cell Biology 2021
Quote:
... Time series images were analyzed using Volocity v6.3 software (Quorum Technologies), and SidK-AL568 molecule fluorescence over time plotted to generate traces where the number of steps required for SidK-AL568 spot disappearance were counted ...
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No products found
because this supplier's products are not listed.
Gwendolyn M. Pais, et al.,
bioRxiv - Pharmacology and Toxicology 2019
Quote:
... including the time in which they resided in metabolic cages (Lab Products Inc. ...
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No products found
because this supplier's products are not listed.
Adam S Hassan, et al.,
bioRxiv - Immunology 2019
Quote:
... plates were washed three times with phosphate buffered saline (PBS: Wisent Bioproducts) and replaced in the incubator with DMEM-FBS containing 50 μg/mL gentamicin (Sigma-Aldrich ...
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No products found
because this supplier's products are not listed.
Roman Franěk, et al.,
bioRxiv - Zoology 2019
Quote:
... and 3 μl PCR H2O (Top-Bio). Reaction conditions were 30 cycles of 94 °C for 30 s ...
-
No products found
because this supplier's products are not listed.
A. Schlör, et al.,
bioRxiv - Immunology 2022
Quote:
... 1 h incubation at RT) and 1-2 μg/mL of HRP-conjugated secondary antibody (ABIN1981272, antibodies-online) in 50 μL/well supplemented with PBS/1% casein or PBS/5 % NCS for 45 min incubation at RT.
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No products found
because this supplier's products are not listed.
Michelle A. Baird, et al.,
bioRxiv - Cell Biology 2023
Quote:
... Cells were incubated for 1.5 hours at RT with the following primary antibodies: LBR (1:200, Atlas Antibodies HPA062236), LaminB1 (1:500 ...
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No products found
because this supplier's products are not listed.
Rémi Porte, et al.,
bioRxiv - Immunology 2024
Quote:
... brain cell suspensions were incubated for 45 min at RT with the following barcoded dextramers diluted in complete RPMI: Kb-SIINFEKL (Immudex cat # JD2163-PfBC ...
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No products found
because this supplier's products are not listed.
John Paul Tsu Ouyang, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... Samples were then shaken at 1500 RPM for five minutes at RT in a benchtop shaker (Benchmark Scientific; Model #: H5000-HC). Samples were then incubated for 5 minutes at RT with no shaking ...
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No products found
because this supplier's products are not listed.
Taylor K Loe, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... Coverslips were dehydrated in an ethanol series (70%, 90% and 100%) and hybridized at RT with a TRITC-OO[TTAGGG]3 labeled PNA probe (PNA Bio) in hybridization buffer (70% formamide ...
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No products found
because this supplier's products are not listed.
Maria V. Sinegubova, et al.,
bioRxiv - Biochemistry 2020
Quote:
... The PCR product was restricted using AbsI (Sibenzyme, Novosibirsk, Russia) and NheI (Fermentas ...
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No products found
because this supplier's products are not listed.
Catherine A. Cotter, et al.,
bioRxiv - Microbiology 2022
Quote:
... wells were washed three times with 250 μl PBS + 0.05% Tween-20 (PBS-T, Accurate Chemical). Plates were blocked for 2 h at room temperature with 200 μl PBS-T + 5% nonfat milk and subsequently washed three times with PBS-T prior to incubation with a series of eight 4-fold dilutions of mouse sera for 1 h at room temperature ...
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No products found
because this supplier's products are not listed.
Clara Lambert, et al.,
bioRxiv - Microbiology 2023
Quote:
... and identified by comparing to retention times of purified esterified FA standards (Mixture ME100, Larodan, Sweden). Results are shown as percent of the specific FA compared to total peak areas (TotalChrom Workstation ...
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No products found
because this supplier's products are not listed.
Aaron Lanz, Grant Gordon, Jaideep Bains,
bioRxiv - Neuroscience 2020
Quote:
... Virus was pressure injected into the brain three times with a Nanoject II apparatus (Drummond Scientific Company), each injection at a volume of 69 nL and placed increasingly deeper into the brain (AP ...
-
No products found
because this supplier's products are not listed.
Elisabet Perez-Coronel, J. Michael Beman,
bioRxiv - Genomics 2021
Quote:
... Temperature and dissolved oxygen were measured at the time of sample collection using a ProODO YSI probe (YSI Inc., Yellow Springs, OH, USA).
-
No products found
because this supplier's products are not listed.
Ricardo J. Ferreira, et al.,
bioRxiv - Microbiology 2021
Quote:
... coli gyrase supercoiling assay kit from Inspiralis (Norwich Research Park ...
-
No products found
because this supplier's products are not listed.
Chengyan Wu, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... The CCK8 kit was purchased from Abbkine. The reactive oxygen detection kit for tissue sections was purchased from Shanghai Pebble Biotechnology Company ...
-
No products found
because this supplier's products are not listed.
Katia K Mattis, et al.,
bioRxiv - Physiology 2022
Quote:
... Single larvae per well of a 96-well PCR plate were homogenised with a 1.4 mm acid washed zirconium bead (OPS diagnostics, USA) in 88 µL ice cold PBS using a MiniG™ 1600 homogenizer (SPEX® SamplePrep ...
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Cat# NOS0500,
For up to 500 assays in 96-well plate : 25 mL reagent 1 (sulf.) + 25 mL reagent 2 (NED) + 1 mL Nitrite Standard (0.1 M), USD $149.00/KIT
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Adam T. Vogel, Shelley J. Russek,
bioRxiv - Neuroscience 2021
Quote:
... using the NeuroMag Magnetofection™ kit (Oz Biosciences #KC30800). Plasmid DNA was diluted in NBM and added to the NeuroMag Transfection Reagent ...
-
No products found
because this supplier's products are not listed.
Shoupeng Wei, et al.,
bioRxiv - Neuroscience 2023
Quote:
Hito Golgi-Cox Kit (Hitobiotec Corp., Wilmington, DE, USA) was used to reveal the structure and density of dendritic spines in the mPFC37–39 ...
-
No products found
because this supplier's products are not listed.
Luca Valzania, Aya Alami, Pierre Léopold,
bioRxiv - Developmental Biology 2023
Quote:
... using the 20-Hydroxyecdysone ELISA kit (Bertin bioreagent, #A05120) as recommended by the manufacturer ...
-
No products found
because this supplier's products are not listed.
Sujoy Chatterjee, et al.,
bioRxiv - Immunology 2020
Quote:
... Gel shift assays were done with EMSA kits (Signosis Inc). 5 μg nuclear extracts were incubated with 1× binding buffer and biotin-labeled probe for 30 min at room temperature ...
-
No products found
because this supplier's products are not listed.
Zhouyi Rong, et al.,
bioRxiv - Neuroscience 2023
Quote:
... Mouse Interferon Gamma (IFNg) ELISA Kit (RD-IFNg-Mu, Reddot biotech), Mouse Interleukin 6 (IL6 ...
-
No products found
because this supplier's products are not listed.
Sybille Koehler, et al.,
bioRxiv - Cell Biology 2020
Quote:
Urinary albumin levels were measured with a mouse albumin ELISA kit (ICL/Dunn Labortechnik GmbH ...
-
No products found
because this supplier's products are not listed.
Syed Moiz Ahmed, et al.,
bioRxiv - Cancer Biology 2019
Quote:
Endogenous TOP1cc were detected by Human Topoisomerase ICE kit (Topogen, TG1020-0) following the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Yuka Sakata, et al.,
bioRxiv - Developmental Biology 2022
Quote:
... Commercially-available clinical-grade enzyme-linked immunosorbent assay (ELISA) kits (DRG International) were used to detect pregnenolone (EIA-4170) ...
-
No products found
because this supplier's products are not listed.
Thomas Klein, et al.,
bioRxiv - Neuroscience 2023
Quote:
... iPSC were generated using the StemRNA 3rd Gen reprogramming kit (Reprocell, Maryland, USA) for all male cell lines (FD-1 ...
-
No products found
because this supplier's products are not listed.
Yonghui Ding, et al.,
bioRxiv - Bioengineering 2023
Quote:
... were expanded in human SMC growth medium kit (Cell Applications, Inc., 311K-500) under the standard culture condition (37 °C with 5% CO2 in a humid environment ...
-
No products found
because this supplier's products are not listed.
Carina C D Joe, et al.,
bioRxiv - Bioengineering 2021
Quote:
Residual host-cell protein (HCP) was quantified using the HEK293 HCP ELISA kit (Cygnus Technologies) according to the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Changrui Lu, et al.,
bioRxiv - Molecular Biology 2022
Quote:
The IgG ELISAs for RBD were conducted by using Antibody IgG Titer Serologic Assay Kit (AcroBiosystems, RAS-T059). According to the manufacturer’s protocol ...