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No products found
because this supplier's products are not listed.
Hua Shen, Ryan A. Lane,
bioRxiv - Bioengineering 2023
Quote:
... and primers specific for mmu-miR-147-3p (alias, hsa-miR-147b; Applied Biosystems). The relative abundance of EV miRNA was determined by TaqMan PCR using TaqMan Universal PCR Master Mix II and TaqMan primers and probes from Applied Biosystems ...
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No products found
because this supplier's products are not listed.
Dayaneth Jácome, et al.,
bioRxiv - Neuroscience 2023
Quote:
... Samples were normalized for the relative expression of the housekeeping miR103a-3p (hsa-miR-103a-3p, LNA™ PCR primer set, Qiagen), which showed no variability between analyzed groups.
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No products found
because this supplier's products are not listed.
Mariska Miranda, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... Standard RT-PCR using independent sets of Predesigned KiCqStart® SYBR® Green Primers (Sigma, Table S11) was carried out for validation with independent biological replicates from 2D cultures (n = 2 ...
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No products found
because this supplier's products are not listed.
Amit Prabhakar, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... qRT-PCR reactions were performed using pre-miR-primers by real-time PCR machine (CFX96, BioRad). 100 U/ml RNase inhibitor (SUPERase•in™ ...
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No products found
because this supplier's products are not listed.
Nikita Vasilyev, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... Index Primers Set 3 (NEB). Amplified libraries were cleaned up using Sample Purification Beads (NEB ...
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No products found
because this supplier's products are not listed.
Yasuo Ariumi,
bioRxiv - Microbiology 2021
Quote:
... real-time RT-PCR was performed with SARS-CoV-2 N primer sets and SYBR Premix Ex Taq II (TaKaRa-Bio) using a LightCycler Nano (Roche ...
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No products found
because this supplier's products are not listed.
Masahiro Nogami, et al.,
bioRxiv - Neuroscience 2021
Quote:
... hsa-miR-125b-5p Pre-miR™ miRNA Precursor (#AM17100, PM10148, ABI), miR-124-3p ...
-
No products found
because this supplier's products are not listed.
Anna Tejchman-Skrzyszewska, et al.,
bioRxiv - Neuroscience 2023
Quote:
... RT-PCR with random primers (Promega, Madison, WI, USA) and Moloney murine leukemia virus M-MLV reverse transcriptase (Promega ...
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No products found
because this supplier's products are not listed.
Yi-Chen Chen, et al.,
bioRxiv - Bioengineering 2023
Quote:
... The DNA fragments around the crRNA target site were amplified by a two-step PCR with specific primer sets (Table S2) and Index PCR Primers mentioned in the manufacturer’s instructions (Illumina, USA). After gel purification ...
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No products found
because this supplier's products are not listed.
Kasper Mikkelsen, et al.,
bioRxiv - Microbiology 2023
Quote:
... RT-PCR reactions were set up using the FastStart Essential DNA Green Master kit (Roche), using four different primer pairs ...
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No products found
because this supplier's products are not listed.
Baojun Ren, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... Mycoplasma Plus PCR Primer Set (Agilent) was used to determine mycoplasma for all cell lines ...
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No products found
because this supplier's products are not listed.
Michael J. Podolsky, et al.,
bioRxiv - Cell Biology 2023
Quote:
... Quantitative real-time PCR reactions were performed with different sets of primers and Sensifast SYBR (Bioline, Taunton, MA) on a CFX96 or CFX384 Real-Time PCR Detection System (Bio-Rad ...
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No products found
because this supplier's products are not listed.
Grant F Marshall, et al.,
bioRxiv - Neuroscience 2023
Quote:
... Primers for reverse-transcription PCR (RT-PCR) and quantitative RT-PCR (qRT-PCR) were supplied by Merck with standard desalting at 100 µM in TE buffer ...
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No products found
because this supplier's products are not listed.
Pirunthan Perampalam, et al.,
bioRxiv - Systems Biology 2020
Quote:
... was used to clone a pool of four sgRNAs per gene (AGPS, SLC2A11, ZC3H7A, PDCD2L, NPM1, EPS15, hsa-mir-761, RPAP1, SYAP1, TRAF3IP1, and EGFP) into lentiCRISPR v2 (Addgene #52961). iOvCa147 cells were transduced with viral particles encoding a Cas9 and sgRNA expression cassettes ...
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No products found
because this supplier's products are not listed.
Noushin Hadadi, et al.,
bioRxiv - Systems Biology 2019
Quote:
... and indexed with ScriptSeq™ Index PCR primers set 1 (Epicentre, Illumina) following the standard protocol ...
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No products found
because this supplier's products are not listed.
Wei Yu, et al.,
bioRxiv - Developmental Biology 2020
Quote:
... Mouse positive control primer set Actb2 (#71017, Active Motif) and mouse negative control primer set 1 (#71011 ...
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No products found
because this supplier's products are not listed.
Yumei Qin, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Genotypes were confirmed using primer sets recommended by Jackson Laboratory and Sinha lab.
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No products found
because this supplier's products are not listed.
Kaneez Fatima, et al.,
bioRxiv - Cell Biology 2024
Quote:
... TransIT-X2 (MirusBio, MIR 6000) or TransIT2020 (Mirus, MIR 5400) were used for cell transfection according to manufacturer’s instruction.
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No products found
because this supplier's products are not listed.
Erin A. Nekritz, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... MiRNA inhibition in MCF-10A cells was achieved by transducing cells with a miRNA inhibitor against hsa-miR-424-5p and hsa-miR-503-5p (Genecopoeia #HmiR-AN0494-AM03 and #HmiR-AN0550-AM03 respectively) ...
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No products found
Molly R. Patterson, et al.,
bioRxiv - Cancer Biology 2024
Quote:
... hsa-miR-203a miRNA mimic (MIMAT0000264) and antagomir (MIMAT0031890) were obtained from ABM.
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No products found
because this supplier's products are not listed.
Karine Pozo, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... and NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 1 E7335S, Index Primers Set 2, E7500S) with Agencourt AMPure XP magnetic beads (Beckman coulter A63881) exactly as per manufacturer’s instructions ...
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No products found
because this supplier's products are not listed.
Leonardo Lupori, et al.,
bioRxiv - Neuroscience 2021
Quote:
... The primer sets used were Quick-16S™ Primer Set V3-V4 (Zymo Research). The sequencing library was prepared using an innovative library preparation process in which PCR reactions were performed in real-time PCR machines to control cycles and therefore limit PCR chimera formation ...
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No products found
because this supplier's products are not listed.
Cilia R Pothast, et al.,
bioRxiv - Immunology 2022
Quote:
... Smartseq2modified PCR primer (Eurogentec) and TRAC or TRBC1/2 specific primers (Eurogentec ...
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No products found
because this supplier's products are not listed.
Isaline Bontemps, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... and 635 nm) or on a BD FACSCalibur (Configuration: 488 nm and 635 nm) (BD Biosciences, San Jose, CA). Data were analyzed with FlowJo v10.7.1 (Tree Star) ...
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No products found
because this supplier's products are not listed.
Coralie Dessauges, et al.,
bioRxiv - Systems Biology 2022
Quote:
... The following primers were used for the RT-qPCR reaction (designed with the Real-time PCR (TaqMan) Primer and Probes Design Tool from GenScript).
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No products found
because this supplier's products are not listed.
Katarzyna Goljanek-Whysall, et al.,
bioRxiv - Physiology 2019
Quote:
... with 100nM miR scrambled or miR-181a mimic (100nM; GE Healthcare) (Soriano-Arroquia et al. ...
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No products found
because this supplier's products are not listed.
Alexandr Samocha, et al.,
bioRxiv - Cell Biology 2020
Quote:
... RT PCR was performed using the RealPlex2 (Eppendorf). Expression was normalized to ß-Actin (Mm02619580_g1 ...
-
No products found
because this supplier's products are not listed.
Astrid Hoermann, et al.,
bioRxiv - Synthetic Biology 2020
Quote:
... RT-PCR was performed with RedTaq Polymerase (VWR) with primers 51-Scorpine-F and 117-CP-ctrl-R on Sco-CP (1267bp) ...
-
No products found
because this supplier's products are not listed.
Shubhangini Tiwari, et al.,
bioRxiv - Neuroscience 2022
Quote:
... The cDNA was obtained by RT-PCR and amplified through PCR using respective primers (OriGene Technologies) followed by agarose gel electrophoresis to assess the gene expression ...
-
No products found
because this supplier's products are not listed.
Rachel E. Turn, et al.,
bioRxiv - Cell Biology 2021
Quote:
... and 635 laser lines) and widefield microscopes (Olympus IX81 microscope and Slidebook software ...
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No products found
because this supplier's products are not listed.
Ekaterina S. Potekhina, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... OneTube RT-PCR SYBR kit (Evrogen) was used for cDNA production by reverse transcription followed by qPCR ...
-
No products found
because this supplier's products are not listed.
Pastor Jullian Fabres, et al.,
bioRxiv - Plant Biology 2021
Quote:
... RT-PCR products were purified using PCR Clean-up (Macherey-Nagel, 740609.250) following the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Anthony D Fenton, et al.,
bioRxiv - Microbiology 2023
Quote:
... the cells were first stained with an anti-HSA antibody (Biolegend) at 1:100 concentration before fixation and analysis ...
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No products found
because this supplier's products are not listed.
Connor G. G. Bamford, et al.,
bioRxiv - Microbiology 2021
Quote:
... these were run as two separate multiplex PCR “pools” (A & B) using the ARTIC version 3 primer set (ARTIC nCoV-2019 V3 Panel, IDT DNA Inc ...
-
No products found
because this supplier's products are not listed.
En Cai, et al.,
bioRxiv - Immunology 2022
Quote:
... IRM images were acquired using a 635/20x excitation filter (Chroma Technology), and reflected light was collected onto the long-pass side of the DV2 imaging path ...
-
No products found
because this supplier's products are not listed.
Zhihua Liu, et al.,
bioRxiv - Microbiology 2020
Quote:
... Reverse transcription with ZIKA virus specific primer (Rev-AAGTGATCCATGTGATCAGTTGATCC) was performed using FastQuant RT Kit (Tiangen). Real-time PCR was done on 7900HT (ABI ...
-
No products found
because this supplier's products are not listed.
Victoria Zyulina, et al.,
bioRxiv - Immunology 2020
Quote:
To silence miR-424 the GFP tagged lentiviral vectors that express antisense hsa-miR-424 (MZIP424-PA-1) and corresponding scramble control lentiviral vectors (MZIP000-PA-1) were purchased from SBI (System Biosciences). Transfection of 293T packaging cell line was performed using calcium-phosphate protocol and infection of target cells was done as previously described (Platzer et al. ...
-
No products found
because this supplier's products are not listed.
Krisztina Percze, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... 130 of the colonies were analysed by colony PCR (using an M13 primer set, Table S1) and capillary electrophoresis (LabChip GX, PerkinElmer) using the DNA 1K Reagent Kit with DNA HT 5K LabChip single sipper chip ...
-
No products found
because this supplier's products are not listed.
Wenyi Zheng, et al.,
bioRxiv - Synthetic Biology 2023
Quote:
... EVs were incubated with FITC-HSA (Abcam, ab8030) at 37 C° for 2 h before SEC separation.
-
No products found
because this supplier's products are not listed.
Minee L. Choi, et al.,
bioRxiv - Neuroscience 2022
Quote:
... A dichroic mirror (DI03-R405/488/561/635, Semrock) reflected the beam through an oil-immersion objective lens (Nikon CFI Plan Apochromat VC 100x Oil ...
-
No products found
because this supplier's products are not listed.
Peng Wang, et al.,
bioRxiv - Cell Biology 2021
Quote:
... and phosphatase inhibitor cocktail set III (Calbiochem). Protein was quantified with Pierce BCA protein assay kit (Thermo Fisher ...
-
No products found
because this supplier's products are not listed.
Justin X. Boeckman, et al.,
bioRxiv - Pathology 2021
Quote:
... Positive bands of appropriate size were confirmed using individual primer sets and resultant PCR products were visualized on a 1.5% agarose gel with gel red (Biotium).
-
No products found
because this supplier's products are not listed.
Dennis J. Doorduijn, et al.,
bioRxiv - Microbiology 2021
Quote:
... + 0.05% human serum albumin (HSA, Sanquin).
-
No products found
because this supplier's products are not listed.
Erick X. Pérez-Guzmán, et al.,
bioRxiv - Microbiology 2019
Quote:
... and using DENV RT primer/probe Mix kit (Genesig, Primerdesign Ltd., UK) according to the manufacturer’s protocol ...
-
No products found
because this supplier's products are not listed.
Zhi Yang, et al.,
bioRxiv - Bioengineering 2023
Quote:
... All qRT-PCR primers were ordered from Eton Bioscience. Quantitative real-time PCR was performed using the SYBR Green Mix (Cat# A25742 ...
-
No products found
because this supplier's products are not listed.
Corinne A. Tovey, et al.,
bioRxiv - Cell Biology 2021
Quote:
... were synthesised either by PCR using two overlapping primers or by GENEWIZ. They were inserted into the linear backbone by HiFi Assembly (NEB) ...
-
No products found
because this supplier's products are not listed.
Teketel A. Haile, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... Two allele specific forward primers and a reverse primer were designed for use in fluorescence based competitive allele-specific PCR assays (KASP; LGC Biosearch Technologies). DNA from the 286 lines was assayed using these primers and KASP reaction mix (LGC Biosearch Technologies ...
-
No products found
because this supplier's products are not listed.
Clay Conner, et al.,
bioRxiv - Cancer Biology 2019
Quote:
... the cells were incubated with DAPI diluted in PBS for 10 min at RT and washed before being mounted onto slides with Vectashield Hard Set (Vector Laboratories). Single-planed images were taken on a Nikon C2 confocal microscope using a 40X oil-immersion lens ...
-
No products found
because this supplier's products are not listed.
Raphael Brandt, et al.,
bioRxiv - Cell Biology 2019
Quote:
Immunofluorescence and FIRE reporter images were taken with a Leica TSC SPE confocal microscope using an ACS 20x oil-immersion objective, solid-state lasers (405, 488, 532 and 635 nm) as sources of excitation and LAS X operating software (Leica). Light microscopy images of cultures were taken with a Biozero microscope using a Plan Apo 4x NA 0.20 objective and Biozero observation and analyser software (Keyence).
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No products found
because this supplier's products are not listed.
Aleksandr Alekseev, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... Filter set 10 (Carl Zeiss) was used for separating the fluorescence and the excitation light ...