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No products found
because this supplier's products are not listed.
Melissa Immerheiser, et al.,
bioRxiv - Microbiology 2021
Quote:
... The Yellow fever NS5 protein was codon-optimised using the algorithms provided by Invitrogen (Thermo Fisher, USA), and a FLAG-tag encoding sequence was added to the carboxyl-terminus ...
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No products found
because this supplier's products are not listed.
James M. Burke, et al.,
bioRxiv - Immunology 2021
Quote:
... cells were incubated with the anti-Influenza A virus NS1 rabbit antibody (GeneTex; GTX125990) at 1:1000 for two hours ...
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No products found
because this supplier's products are not listed.
Romel Rosales Ramirez, Juan E. Ludert,
bioRxiv - Molecular Biology 2019
Quote:
The NS1 sequence from ZIKV (Mexican isolate, Asiatic linkage) and YFV NS1 (Brazilian yellow fever virus isolate) were synthesized de novo (GenScript, Piscataway, NJ). The synthetic ZIKV NS1 gene (GenBank accession number KY631493.1 ...
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No products found
because this supplier's products are not listed.
Ann Logan, et al.,
bioRxiv - Immunology 2022
Quote:
... and yellow fever E antibody 3576 (Santa Cruz, sc-58083, for YF17D). The primary antibodies were detected with an Alexa-488 conjugate secondary antibody (Life Technologies ...
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No products found
because this supplier's products are not listed.
Oliver Brookes, et al.,
bioRxiv - Cell Biology 2021
Quote:
Lucifer Yellow Carbohydrazide (Sigma L0259) was prepared as a 1mg.mL-1 stock solution in HBSS with calcium and magnesium (Gibco ...
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No products found
because this supplier's products are not listed.
Michele Martins, et al.,
bioRxiv - Biochemistry 2020
Quote:
... it was screened for Dengue virus (DENV) using the Dengue NS1 Ag STRIP (Bio-Rad). The use of human blood was approved by the Fiocruz Ethical Committee (CAAE 53419815.9.0000.5248).
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No products found
because this supplier's products are not listed.
Ioanna A. Rota, et al.,
bioRxiv - Immunology 2021
Quote:
... green fluorescence protein (GFP) or an enhanced yellow fluorescent protein (EYFP) sequence (pcDNA1.3-, Addgene) using standard cloning methods ...
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No products found
because this supplier's products are not listed.
Madeline J. Price, et al.,
bioRxiv - Immunology 2021
Quote:
... Zombie Yellow (BV570) (Biolegend, 77168), and CTV (Life Technologies ...
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No products found
because this supplier's products are not listed.
Jonas N. Conde, et al.,
bioRxiv - Microbiology 2023
Quote:
... and recLI9-NS1-GFP11 reporter virus was purified using RNeasy kit (Qiagen). cDNA synthesis was performed as above using random hexamer primers (25°C for 10 min ...
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No products found
because this supplier's products are not listed.
Shai Saroussi, et al.,
bioRxiv - Plant Biology 2022
Quote:
... a YSI 5331A electrode (Yellow Springs Instruments, Yellow Springs, OH, USA) polarized at -0.8 V ...
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No products found
because this supplier's products are not listed.
Javier Gilbert-Jaramillo, et al.,
bioRxiv - Neuroscience 2022
Quote:
... and 1:100 anti-NS1 (Abcam; ab214337) was conducted prior to secondary staining with 1:1000 goat anti-mouse Alexa Fluor 546 and goat anti-rabbit Alexa Fluor 750 ...
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No products found
because this supplier's products are not listed.
Nicholas R. DesLauriers, et al.,
bioRxiv - Biophysics 2019
Quote:
... cDNAs encoding cyan fluorescent protein (CFP) and yellow fluorescent protein (YFP) were purchased from Clontech Com ...
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No products found
because this supplier's products are not listed.
Laurie-Anne Lamotte, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... together with Anti-Influenza A Non-Structural Protein 1 (NS1) Mouse Monoclonal Antibody (EMB005, Kerafast). Cells were then washed and incubated with a mixture of secondary antibodies ...
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No products found
because this supplier's products are not listed.
Nipun Verma, et al.,
bioRxiv - Genetics 2024
Quote:
... Virus was concentrated using PEG virus precipitation (Promega # V3011). In brief ...
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No products found
because this supplier's products are not listed.
Mohini Bhupathi, et al.,
bioRxiv - Bioengineering 2024
Quote:
... Typhi (the bacterial pathogen that causes typhoid fever) using 2X Warmstart colorimetric LAMP (M1800, NEB, US). The RNA template of the SARS-CoV-2 N gene was synthesised using in-vitro transcription (Hiscribe ...
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No products found
because this supplier's products are not listed.
Sophia Bakola, et al.,
bioRxiv - Neuroscience 2021
Quote:
... diamidino yellow dihydrochloride (DY, Polysciences) and fast blue (FB ...
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No products found
because this supplier's products are not listed.
Ruobing Wang, et al.,
bioRxiv - Cell Biology 2021
Quote:
... and detected with Discovery Yellow (Roche); counter stained with hematoxylin ...
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No products found
because this supplier's products are not listed.
Adi Miriam Goldenberg, et al.,
bioRxiv - Neuroscience 2020
Quote:
... a short protein sequence expressed in the injected virus (1:400 rabbit anti P2A; ABS31, Merck, Darmstadt, Germany). In the third step ...
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No products found
because this supplier's products are not listed.
A.A. Shaldzhyan, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... Recombinant NS1-6xHis protein was further purified by gel filtration using an AKTA pure chromatograph (GE Healthcare, USA). An XK 16/60 Chromatographic Column ...
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No products found
because this supplier's products are not listed.
Rebecca P. Sumner, et al.,
bioRxiv - Immunology 2023
Quote:
... Sendai virus was from Charles River Laboratories ...
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No products found
because this supplier's products are not listed.
Pei Xuan Lee, et al.,
bioRxiv - Microbiology 2020
Quote:
... Purified NS1 protein from D2Y98P or 16681 (10 ng/well) diluted in PBS was coated onto 96-well EIA plates (Corning costar) overnight at 4 °C ...
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No products found
because this supplier's products are not listed.
Miranda L. Curtiss, et al.,
bioRxiv - Immunology 2022
Quote:
... Biotinylated recombinant NS1 was purified by FPLC and then tetramerized to fluorochrome-conjugated streptavidin (Prozyme). To detect NS1-specific B cells ...
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No products found
because this supplier's products are not listed.
Daniel Perez-Zsolt, et al.,
bioRxiv - Microbiology 2021
Quote:
... Virus particles were immunostained with rabbit anti-SARS-CoV-2 N protein (Sino Biological) or rabbit anti-GM1 Ab (Abcam ...
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No products found
because this supplier's products are not listed.
Kristina Lanko, et al.,
bioRxiv - Microbiology 2020
Quote:
Viral RNA was isolated from virus stocks with Nucleospin RNA virus (Macherey-Nagel) according to manufacturer protocol ...
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No products found
because this supplier's products are not listed.
Carolina Rodriguez-Tirado, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... Data acquisition was performed on a LSR II Yellow (BD Biosciences) and analyzed on FlowJo version 10 (FlowJo ...
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No products found
because this supplier's products are not listed.
Vincent Basse, et al.,
bioRxiv - Microbiology 2021
Quote:
... Purified NS1 was then concentrated using Vivaspin columns (Sartorius).
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No products found
because this supplier's products are not listed.
Zehavit Shapira, et al.,
bioRxiv - Biophysics 2021
Quote:
... 0.06 μm Carboxyl FPS yellow particles (Spherotech) were briefly sonicated and diluted right before use in Dulbecco’s Phosphate Buffered Saline (DPBS ...
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No products found
because this supplier's products are not listed.
Kelly Mitchell, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... and virus was concentrated with PEG-it Virus Precipitation solution (System Biosciences). SORE6-GFP virus was added to CSCs plated on Geltrex ...
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No products found
because this supplier's products are not listed.
Daniel Cabrera, Michael W. Young, Sofia Axelrod,
bioRxiv - Physiology 2020
Quote:
... 5.25% (w/v) yellow cornmeal (VWR #IC90141180), 2.2% (w/v ...
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No products found
because this supplier's products are not listed.
Alexis S. Gorin, et al.,
bioRxiv - Neuroscience 2023
Quote:
... Virus was injected iontophoretically (Nanoject II, Drummond Scientific) via a glass micropipette (tip diameter ∼15-20 μm ...
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No products found
because this supplier's products are not listed.
A.A. Shaldzhyan, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... one milliliter of NS1 recombinant protein (3 mg/ml in 2x PBS) was placed into a 1.5 ml Eppendorf tube (Eppendorf, Germany); and the tube mixed for 66 hours (55°C ...
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No products found
because this supplier's products are not listed.
Jonathan Burnie, et al.,
bioRxiv - Microbiology 2021
Quote:
The quantification of HIV-1 p24 capsid protein was performed in captured virus lysates and virus-containing supernatants with the high-sensitivity AlphaLISA p24 detection kit following the manufacturer’s (PerkinElmer) instructions ...
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No products found
because this supplier's products are not listed.
S.N. Freda, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Virus was injected using a microsyringe pump (World Precision Instruments); >5 minutes elapsed before the pipette was moved from the ventral injection site to the more dorsal injection site ...
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No products found
because this supplier's products are not listed.
Elaine Cristina Matos Vicentin, et al.,
bioRxiv - Immunology 2020
Quote:
... of 5 μg of each mAb (αDEC-NS1, αDCIR2-NS1, αDEC or αDCIR2) together with 50 μg poly (I:C) (Invivogen), exactly as described in (17) ...
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No products found
because this supplier's products are not listed.
Ioannis Kanakis, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... expressing yellow fluorescent protein (YFP) only in neuronal cells (Jackson Laboratory; stock number 003709) were used in this study ...
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No products found
because this supplier's products are not listed.
Nathaniel Kastan, et al.,
bioRxiv - Developmental Biology 2020
Quote:
... Ad-RFP virus (Vector Biolabs) at the same titer were used as a control.
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No products found
because this supplier's products are not listed.
John P. Gleeson, et al.,
bioRxiv - Bioengineering 2021
Quote:
... Fixable Yellow samples were subsequently fixed using Flow Cytometry Fixation Buffer (R&D Systems), centrifuged ...
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No products found
because this supplier's products are not listed.
Kristen Zarrella, et al.,
bioRxiv - Microbiology 2023
Quote:
... Cells were infected (MOI=1) with WT or UL138STOP virus for 48 hours and then processed for CUT&RUN (Cell Signaling Technologies) as per manufacturer’s recommended protocol ...
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No products found
because this supplier's products are not listed.
Lok R. Joshi, et al.,
bioRxiv - Immunology 2021
Quote:
... ultra-centrifugation of virus culuture supernatant and the virus pellet in 30% sucrose cushion gradient were performed using Optima-L 100K ultracentrifuge (Beckman Coulter) at 18,000 RPM for 1.5 hours ...
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No products found
because this supplier's products are not listed.
Andrii Slonchak, et al.,
bioRxiv - Microbiology 2021
Quote:
... Virus replication foci were counted using the Image Studio Lite software (v5.2.5, LI-COR, USA), and titres were determined based on dilution factors and expressed as focus forming units per mL (FFU mL^−1).
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No products found
because this supplier's products are not listed.
Krisztina Ötvös, et al.,
bioRxiv - Plant Biology 2019
Quote:
For BiFC assays Yellow Fluorescent Proteins (YFP) were visualized using a Leica TCS-SP2 laser scanning confocal microscope (LSM) (Leica Microsystems, Heidelberg, Germany) with a 63×1.4 NA oil-objective lens and processed using Leica confocal software v.2.61 about 16 h post-transformation ...
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No products found
because this supplier's products are not listed.
Jingyi Guo Fuglstad, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Fluorescent signal from the virus was amplified by immunostaining against Red Fluorescent Protein (RFP) (catalog no.5F8, Chromotek GmbH, Germany), followed by secondary antibody-staining with Alexa 546-tagged Goat Anti-rat IgG (catalog no ...
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No products found
because this supplier's products are not listed.
Iktae Kim, et al.,
bioRxiv - Biophysics 2022
Quote:
The binding of surface-immobilized NS1 to p85β was measured at 25 °C using an Octet RED biolayer interferometry (Pall ForteBio). The N-terminal His6 and SUMO-tagged NS1 proteins were used for immobilization ...
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No products found
because this supplier's products are not listed.
Michael B. Fessler, et al.,
bioRxiv - Immunology 2022
Quote:
... The virus sequence was confirmed by Illumina sequencing and did not differ from the parental virus ...
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No products found
because this supplier's products are not listed.
Sanjib Saha, et al.,
bioRxiv - Cell Biology 2022
Quote:
... Protein expression was normalized to total protein (DM-TP01, Protein Simple) and compared between cell types.
-
Recombinant Antigen
Cat# YFV-NS1-100,
100µg USD $347.0
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Pei Xuan Lee, et al.,
bioRxiv - Microbiology 2020
Quote:
... 10 mg/kg of purified NS1(D2Y98P) (The Native Antigen Company) or OVA protein (InvivoGen ...
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No products found
because this supplier's products are not listed.
Kuan-Hung Lin, et al.,
bioRxiv - Cell Biology 2023
Quote:
... and the virus titer was measured based on the titer of the viral protein p24 with QuickTiterTM Lentivirus Titer Kit (Cell Biolabs) following the manufacturer’s instructions.
-
No products found
because this supplier's products are not listed.
Takanobu Tagawa, et al.,
bioRxiv - Microbiology 2022
Quote:
... The virus pellet was resuspended to EBM2 (Lonza) media ...
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No products found
because this supplier's products are not listed.
Camilla Schinner, et al.,
bioRxiv - Cell Biology 2021
Quote:
... virus particle containing supernatant was collected and enriched using LentiConcentrator (OriGene). Cells were transduced with the respective concentrated virus particles using 10 µg/mL polybrene (Sigma-Aldrich ...
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No products found
because this supplier's products are not listed.
Marion Thépaut, et al.,
bioRxiv - Biochemistry 2020
Quote:
... Proteins were detected using InstantBlue protein stain (Expedeon) according to the supplier’s instructions.