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No products found
because this supplier's products are not listed.
Irrem-Laareb Mohammad, et al.,
bioRxiv - Biophysics 2022
Quote:
... Protein expression was induced with 1mM of IPTG (Nzytech) and performed for 5 h at 28ºC ...
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No products found
because this supplier's products are not listed.
Vinay Tripuraneni, et al.,
bioRxiv - Genomics 2019
Quote:
Wild-type cells were grown to an OD600 of approximately 0.2-0.3 in YEP with 2% raffinose and 0.1% dextrose at which point alpha factor was added at a final concentration of 50 ng/ml for 3.5-4 h (GenWay). 20% galactose was added to a final concentration of 2% in the medium to induce HO expression ...
-
No products found
because this supplier's products are not listed.
Amalia Sintou, et al.,
bioRxiv - Immunology 2019
Quote:
... Apoptosis assays were performed using Nucleocounter NC-3000 and a Flexicyte apoptosis/necrosis detection kit based on staining with caspase 3 substrate NucView 488 and RedDot 2 (all Biotium, Cambridge Bioscience, UK). Apoptotic cells are defined as NucView488 positive and RedDot2 negative ...
-
No products found
because this supplier's products are not listed.
Nicholas G. Schott, et al.,
bioRxiv - Bioengineering 2024
Quote:
... and 10 ng/mL fibroblast growth factor 2 (Shenandoah Biotechnology, Part # 100-146). MSC used in subsequent experiments were at passage 6 (9-11 population doublings).
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No products found
because this supplier's products are not listed.
Erin E. Henninger, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... α factor (Bachem) was added to a final concentration of 10-7 M and cultures were incubated for 130 minutes at 30°C ...
-
No products found
because this supplier's products are not listed.
Michael J. Pereira, et al.,
bioRxiv - Microbiology 2021
Quote:
... Classical pathway-mediated complement activation was then induced by adding 2% final pooled Normal Human Serum (Innovative Research) and a two-fold dilution series of GST-ErpB/GST-ErpQ/GST-BB0460 or untagged proteins BBK32-C/ErpQ19-343 ...
-
No products found
because this supplier's products are not listed.
Chun Hu, et al.,
bioRxiv - Biochemistry 2021
Quote:
... Protein expression was induced by baculovirus infection of 6-8 liter cultures of Sf9 cells in ESF921 medium (Expression Systems) at a density of ~2 × 106 cells/ml ...
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No products found
because this supplier's products are not listed.
Giacomo Pirovano, et al.,
bioRxiv - Cancer Biology 2019
Quote:
... was injected intracranially using the same coordinates as for tumor cell injection (2 mm lateral and 1 mm posterior to the bregma using a Stoelting Digital New Standard Stereotactic Device and a 5 μL Hamilton syringe) ...
-
No products found
because this supplier's products are not listed.
Soumen Barman, et al.,
bioRxiv - Biochemistry 2022
Quote:
... Protein expression was induced overnight at 17°C with 0.5 mM IPTG in an Innova 44 Incubator shaker (New Brunswick Scientific). Proteins were purified as follows ...
-
No products found
because this supplier's products are not listed.
Veronica Gonzalez, et al.,
bioRxiv - Genomics 2021
Quote:
... that contained alpha-thio-ddNTPs (Trilink Bio Technologies) at equal ratios at a concentration of 1200 μM in the final amplification reaction ...
-
No products found
because this supplier's products are not listed.
Jun Kunimatsu, Hidetoshi Amita, Okihide Hikosaka,
bioRxiv - Neuroscience 2023
Quote:
... a tungsten electrode (Alpha Omega Engineering or FHC) was lowered into the striatum through a guide tube using a micromanipulator (MO-97S ...
-
No products found
because this supplier's products are not listed.
Giorgia Fedele, et al.,
bioRxiv - Cell Biology 2022
Quote:
... 5 U/mL Heparin and 150 μg/mL Endothelial Cell Growth Factor on 2% gelatin-coated dishes (Euroclone). Human astrocytes (HA ...
-
No products found
because this supplier's products are not listed.
Zihao Wang, et al.,
bioRxiv - Molecular Biology 2024
Quote:
... pH 7.0 (The Protein Complex Suite F1, Molecular Dimensions, protein: precipitant ratio 2:1). The crystal was harvested and flash-cooled without adding cryo-protectant ...
-
No products found
because this supplier's products are not listed.
Benedikt K. Rossboth, et al.,
bioRxiv - Biophysics 2019
Quote:
Monomeric photoswitchable cyan fluorescence protein 2 (PS-CFP2, Evrogen) was C-terminally equipped with an AVI-tag (GLNDIFEAQKIEWHE ...
-
No products found
because this supplier's products are not listed.
Katarzyna M. Grochowska, et al.,
bioRxiv - Neuroscience 2022
Quote:
... For Aβ-induced CREB shutoff experiments Aβ oligomers (Anaspec) were prepared as described previously (see previous section and (Grochowska et al. ...
-
No products found
because this supplier's products are not listed.
V Paradise, et al.,
bioRxiv - Neuroscience 2022
Quote:
... induced with Isopropyl β-D-1-thiogalactopyranoside (Hello Bio), and incubated30 ...
-
No products found
because this supplier's products are not listed.
Eric A. Kirk, et al.,
bioRxiv - Neuroscience 2023
Quote:
... Anesthesia was induced with isoflurane (1-5%, Kent Scientific), eye lubricant was applied ...
-
No products found
because this supplier's products are not listed.
Jip Zonderland, Silvia Rezzola, Lorenzo Moroni,
bioRxiv - Bioengineering 2019
Quote:
... or basic fibroblast growth factor (bFGF) (Neuromics), ethanol sterilized ESP scaffolds were incubated in 0,5M NaOH for 30min at room temperature to open the ester bond of the 300PEOT55PBT45 polymer ...
-
No products found
because this supplier's products are not listed.
Jakob Farnung, et al.,
bioRxiv - Biochemistry 2022
Quote:
... Protein solution (2 μL) was mixed with reservoir solution (2 μL) on siliconized cover-slips (Hampton Research). Crystallizations were performed at room temperature ...
-
No products found
because this supplier's products are not listed.
Elena Kuzmin, et al.,
bioRxiv - Genomics 2023
Quote:
... 10 ng/mL Epidermal growth factor (BPS bioscience), 10 umol/L Y-27632 (Abmole) ...
-
No products found
because this supplier's products are not listed.
Anne Van Arsdale, et al.,
bioRxiv - Cancer Biology 2019
Quote:
... tumor genomic DNA was mechanically fragmented to 200 bp (Covaris, Woburn, MA), and Illumina adaptors were ligated at each end ...
-
No products found
because this supplier's products are not listed.
George Cameron, et al.,
bioRxiv - Biophysics 2022
Quote:
... Anti-GINS antibody was affinity purified using protein A-sepharose (Covalab). For bulk replication assays ...
-
No products found
because this supplier's products are not listed.
Moumita Sarkar, et al.,
bioRxiv - Developmental Biology 2021
Quote:
... pCDNA-T292A/S294A and pCDNA-Cnot3 Δ651-751 constructs were in vitro transcribed/translated as described above and the resultant V5 tagged proteins were purified using V5-tagged protein purification kit ver.2 (MBL International, USA). Purified proteins were used as substrate and incubated with 80 ng of GST-Aurora B (ProQuinase ...
-
No products found
because this supplier's products are not listed.
Xin Zhang, et al.,
bioRxiv - Pharmacology and Toxicology 2020
Quote:
Ligand-induced accumulation of cAMP was quantified using the cAMP dynamic Gs kit (Cisbio). Cells were pelleted and washed once in Dulbecco’s Phosphate Buffered Saline (DPBS ...
-
No products found
because this supplier's products are not listed.
Marc A. Vittoria, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... 10 mg of tumor tissue was dissected and placed into RIPA lysis buffer (Boston BioProducts) supplemented with one Pierce protease inhibitor tablet and 1X HALT (Thermo Fisher) ...
-
No products found
because this supplier's products are not listed.
Audry Fernandez, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... Total number of cells in tumors and spleens were determined using SPHERO AccuCount Fluorescent Particles (Spherotech, IL). Expression of H-2Kb and PD-L1 were assayed in the CD45− fraction generated from WT and CCN4-KO YUMM1.7 tumors using the antibodies ...
-
No products found
because this supplier's products are not listed.
Thomas O’Loughlin, et al.,
bioRxiv - Cell Biology 2019
Quote:
Cells were plated on alpha-numeric gridded glass-bottom coverslips (P35G-1.5-14-C-GRID, MatTek, MA, USA) at ∼40-50% confluency and fixed with 2% formaldehyde ...
-
No products found
because this supplier's products are not listed.
Ming Wu, et al.,
bioRxiv - Pathology 2023
Quote:
... Cre recombinase activity was induced by intraperitoneally injection of tamoxifen (50 mg kg-1 day-1, Apexbio, B5965) for three consecutive days at different time point according to the experimental design which is indicated in the result part.
-
Alpha-arbutin (alpha-Arbutoside, 4-hydroxyphenyl-D-lucopyranoside) is a synthetic and functional...
Cat# S5112, SKU# S5112-25mg,
25mg, $97.00
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Valerio E.C. Piscopo, et al.,
bioRxiv - Cell Biology 2023
Quote:
... iPSCs are induced towards neural progenitors for 8 DIV using SB31542 (10µM) and DMH-1 (2µM, Selleck Chemicals LLC), patterned with Retinoic Acid (100nM ...
-
No products found
because this supplier's products are not listed.
Lena Wiedmann, et al.,
bioRxiv - Cancer Biology 2022
Quote:
Blood was taken from the vena facialis 11 days after tumor injection and collected in a microvette LH (Sarstedt, 201.345) to receive the serum ...
-
No products found
because this supplier's products are not listed.
Kizzmekia S. Corbett, et al.,
bioRxiv - Immunology 2020
Quote:
HEK293T cells were transiently transfected with mRNA encoding SARS-CoV-2 WT S or S-2P protein using a TranIT mRNA transfection kit (Mirus). After 24 hr ...
-
No products found
because this supplier's products are not listed.
Francesca Taglini, et al.,
bioRxiv - Genetics 2023
Quote:
... 2 μl /1×106 cells of the following antibodies were used for immunoprecipitations: H3K4me3 (EpiCypher 13-00041), H3K9me3 (Active Motif 39161) ...
-
No products found
because this supplier's products are not listed.
Maria Font Farre, et al.,
bioRxiv - Plant Biology 2023
Quote:
... 44.5 μL of labelled proteins were incubated with 2 μM Cy5 Picolyl Azide (Click Chemistry Tools, 50 μM stock in DMSO) or Fluorescein Picolyl Azide (Click Chemistry Tools ...
-
No products found
because this supplier's products are not listed.
Georg Hafner, et al.,
bioRxiv - Neuroscience 2020
Quote:
... Anesthesia was induced with 3% isoflurane (vol/vol) and maintained between 0.5 and 1% throughout the entire surgical procedure (Harvard Apparatus, USA). Mice were mounted on a custom-built frame with rigid earbars ...
-
No products found
because this supplier's products are not listed.
Jérôme Montnach, et al.,
bioRxiv - Pharmacology and Toxicology 2023
Quote:
... Low resistance borosilicate glass pipettes (2-3 MΩ; Sutter Instruments) were filled with a solution containing (in mM) ...
-
No products found
because this supplier's products are not listed.
Ugo Dionne, et al.,
bioRxiv - Systems Biology 2020
Quote:
... Protein production was then induced by the addition of 1 mM IPTG (Bio Basic) and incubation at 16°C overnight with agitation ...
-
No products found
because this supplier's products are not listed.
Sachin Kumar, et al.,
bioRxiv - Bioengineering 2020
Quote:
... fibrinogen (FIB 2) 7.5 mg/ml and thrombin (Factor IIa) 1.0 U/ml (Enzyme Research Laboratories)) [7] was prepared ...
-
No products found
because this supplier's products are not listed.
Resti Rudjito, et al.,
bioRxiv - Neuroscience 2021
Quote:
CAIA was induced by intravenous (i.v.) injection of a collagen type II (CII) arthritogenic antibodies (1.5 mg/mouse, Chondrex) on day 0 followed by intraperitoneal (i.p. ...
-
No products found
because this supplier's products are not listed.
Kathie L. Eagleson, et al.,
bioRxiv - Neuroscience 2020
Quote:
... with Alpha-Dri bedding (Shepherd Specialty Papers, MI) and one standard cotton fiber nestlet square (Ancare Corp. ...
-
No products found
because this supplier's products are not listed.
Michael G. Spelios, et al.,
bioRxiv - Immunology 2021
Quote:
... A SARS-CoV-2 neutralization antibody (EpiGentek), which targets the spike RBD ...
-
No products found
because this supplier's products are not listed.
Aurélie de Rus Jacquet, et al.,
bioRxiv - Pharmacology and Toxicology 2021
Quote:
The following antibodies were used in this study: chicken anti-microtubule-associated protein 2 (MAP2) (catalog number CPCA-MAP2, EnCor Biotechnology, Gainesville, FL); rabbit anti-tyrosine hydroxylase (TH ...
-
No products found
because this supplier's products are not listed.
Mayis Kaba, et al.,
bioRxiv - Cell Biology 2022
Quote:
... Alpha Fluor 488 amine (20 μg/mL, AAT Bioquest/Cat No. 1705) was added at room temperature for 30 min with agitation before overnight incubation with hIgG ...
-
No products found
because this supplier's products are not listed.
Qiao Wang, et al.,
bioRxiv - Pathology 2022
Quote:
... Precipitated proteins were detected with an anti-GFP antibody (#A02020; Abbkine), an anti-mCherry antibody (#A02080 ...
-
No products found
because this supplier's products are not listed.
Miguel Á. Muñoz-Alía, et al.,
bioRxiv - Microbiology 2022
Quote:
... Amino acid substitutions and deletions in the SARS-CoV-2 spike protein (shown in Figure 2) were introduced using standard molecular biology techniques135 and confirmed by Sanger sequencing (GENEWIZ). When indicated ...
-
No products found
because this supplier's products are not listed.
Apurva A. Govande, et al.,
bioRxiv - Microbiology 2023
Quote:
... Antibodies against the following proteins were used: ABCF1 (Aviva Systems biology; ARP43631_P050), ABCF3 (Sigma ...
-
No products found
because this supplier's products are not listed.
Colbie R. Chinowsky, et al.,
bioRxiv - Cell Biology 2020
Quote:
... Induced W4 cells were then incubated with either 20 µM Blebbistatin (B592500; Toronto Research Chemicals), 4 nM Calyculin A (PHZ1044 ...
-
No products found
because this supplier's products are not listed.
S. ter Horst, et al.,
bioRxiv - Microbiology 2022
Quote:
2’-Fluoro-2’-deoxycytidine (2’-FdC) (Biosynth-Carbosynth) was dissolved in 100% DMSO (VWR Chemicals) ...
-
No products found
because this supplier's products are not listed.
Lisa Leib, et al.,
bioRxiv - Molecular Biology 2024
Quote:
... Starvation of cells was induced by washing the cells four times with Hanks’ Balanced Salt solution (HBSS; PAN Biotech; #P04-32505) for the indicated time points ...
-
No products found
because this supplier's products are not listed.
Suo Han, et al.,
bioRxiv - Cell Biology 2023
Quote:
... The blot was blocked with 5% skim milk solution for 2 h at room temperature and incubated with primary POP antibody (dilution 1: 1000, BS60084, BIOWORLD), TGF-β1(dilution 1:500 ...
-
No products found
because this supplier's products are not listed.
Peter Koppensteiner, et al.,
bioRxiv - Neuroscience 2022
Quote:
... in 2% BSA in TBS at 4 °C for 2 O/N and respective 1.4 nm gold-conjugated secondary antibodies (Nanoprobes Inc., USA) for silver intensification or in biotinylated anti-rabbit secondary antibody (Vector Labs ...