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Citations for Click Chemistry Tools :
1 - 50 of 52 citations for Tumor Necrosis Factor Alpha Induced Protein 2 TNFAIP2 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... 44.5 μL of labelled proteins were incubated with 2 μM Cy5 Picolyl Azide (Click Chemistry Tools, 50 μM stock in DMSO) or Fluorescein Picolyl Azide (Click Chemistry Tools ...
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bioRxiv - Plant Biology 2024Quote: ... 44.5 μl of labelled proteins were treated with 2 μM Cy5 Picolyl Azide (Click Chemistry Tools, 50 μM stock in DMSO) or Fluorescein Picolyl Azide (Click Chemistry Tools ...
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bioRxiv - Biochemistry 2020Quote: ... Lysates containing 50 μg of protein were then incubated for 2 h with shaking at 37°C with 100 μM TAMRA azide (Click Chemistry Tools Scottsdale, Az), 800 μM copper(II ...
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bioRxiv - Biochemistry 2020Quote: ... Enrichment of alkyne-modified proteins was conducted using the Click-&-Go Protein Enrichment Kit (Click Chemistry Tools) and the protocols thereof ...
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bioRxiv - Microbiology 2020Quote: ... to AHA containing proteins was carried out using Click-&-Go™ Protein Reaction Buffer Kit (Click Chemistry Tools) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 mM THPTA (Click Chemistry Tools), and 100 µM copper sulfate ...
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bioRxiv - Physiology 2023Quote: ... Enriched proteins were eluted for mass spectrometry analysis according to the Click-&-GoTM Dde Protein Enrichment Kit (Click Chemistry Tools, 1153).
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bioRxiv - Molecular Biology 2021Quote: ... 2 mM biotin azide (Click Chemistry Tools), 10 mM sodium ascorbate made fresh (Sigma) ...
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bioRxiv - Biochemistry 2022Quote: ... Fluorophores for protein labeling were from Click Chemistry Tools Inc.
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bioRxiv - Molecular Biology 2022Quote: ... 2 mM THPTA (Click Chemistry Tools 1010-100), and 100 µM copper sulfate (Fisher Scientific AC197722500) ...
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bioRxiv - Microbiology 2023Quote: ... using the Click-&-Go protein enrichment kit (Click Chemistry Tools) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2021Quote: ... 2 μM Cy3 azide (Click Chemistry Tools AZ119-1), and 100 mM ascorbic acid freshly prepared and diluted in Gomori buffer (pH 7.4) ...
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bioRxiv - Genetics 2022Quote: ... 5-ethynyl-2’-deoxyuridine (EdU) (Click Chemistry Tools 1381, Scottsdale, AZ) was added to a final concentration of 10 uM ...
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bioRxiv - Microbiology 2022Quote: ... with 2 μM Alexa Fluor 488 azide (Click Chemistry tools #1275) according to manufacturer instructions ...
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bioRxiv - Neuroscience 2022Quote: ... 20 µM 5-Ethynyl-2′-deoxyuridine (EdU; Click Chemistry Tools 1149) was added to the cell culture media for 30 minutes to label NSCs in S-phase ...
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bioRxiv - Neuroscience 2020Quote: ... Alkyne-conjugated biotin and Protein Reaction Buffer kit were from Click Chemistry Tools.
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bioRxiv - Biochemistry 2021Quote: Protein pellets functionalized with the TAMRA-Azide-Desthiobiotin (Click Chemistry Tools, Scottsdale, AZ) were precipitated by methanol/chloroform as described above and resuspended in 500 μL of 6M Urea + 25 mM ammonium bicarbonate ...
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bioRxiv - Cancer Biology 2020Quote: ... 5 μl of 2 mM biotin-alkyne-tag (Click Chemistry Tools, 1266), and 5 μl of 200 mM CuSO4 were added in the specified order to 5 ml of PBS (pH 7.8) ...
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bioRxiv - Biophysics 2023Quote: ... for further conjugation with DBCO-PEG4-NHS (Click Chemistry Tools, A134-2) via succinimidyl ester chemistry ...
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bioRxiv - Cell Biology 2021Quote: ... was conducted using the Click-&-Go Protein Reaction Buffer Kit (Click Chemistry Tools 1262) according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2024Quote: ... All fluorophores used to generate fluorescently labeled proteins were purchased from Click Chemistry Tools. Cy3-labeled ...
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bioRxiv - Bioengineering 2021Quote: ... 50 μL of 1 mg/mL antibodies were reacted with 1 μL of 10 mM UV-cleavable azido-NHS ester (30 equivalents to antibodies; Click Chemistry Tools) for 2 h ...
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bioRxiv - Cancer Biology 2024Quote: ... Antibodies were conjugated to mTz-PEG4-NHS (Click chemistry tools) at first in 1x BBS ...
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bioRxiv - Microbiology 2022Quote: ... AF 488 picolyl azide and 5-ethynyl-2’-deoxycytidine (EdC) (Click Chemistry Tools, Scottsdale, AZ) were used for click chemistry according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2023Quote: ... AlexaFlour 488 picolyl azide and 5-ethynyl-2’-deoxycytidine (EdC) (Click Chemistry Tools, Scottsdale, AZ) were used for click-chemistry according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2020Quote: ... The Click-iT reaction was performed with the Click-&-Go Protein Reaction Buffer Kit (Click Chemistry Tools) and AF488 alkyne (Click Chemistry Tools ...
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bioRxiv - Immunology 2023Quote: ... beads (now containing bound protein) were washed and incubated with 5 μM DBCO-488 (Click Chemistry Tools) for 30 min at RT or overnight at 4°C ...
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Modifications in the T arm of tRNA globally determine tRNA maturation, function and cellular fitnessbioRxiv - Molecular Biology 2023Quote: ... Cell opening and fluorescent labeling of L-AHA-containing proteins with DBCO-PEG4-Carboxyrhodamine 110 (Click Chemistry Tools) were performed as described 29 ...
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bioRxiv - Biophysics 2024Quote: ... IEDDA reaction was performed by applying 1-2 μM tetrazine dye (Click Chemistry Tools, Scottsdale, AZ) to the culture medium and incubating it with the cells at 37°C for 30 minutes ...
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bioRxiv - Molecular Biology 2020Quote: ... Antibody was modified with DBCO-Sulfo-NHS ester (Click Chemistry Tools, #A124) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Click-labeling of nascent proteins was performed with 8.3 µM dibenzocyclooctyne (DBCO)−488 (Click Chemistry Tools Catalog #1278-5) in PBS for 30 minutes at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... In every replicate 1 – 2 mg BTD-labeled lysate was clicked to Az800 (AzDye 800 – Click Chemistry tools) as described above ...
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bioRxiv - Bioengineering 2022Quote: ... and 5-days post-surgery along with 5-Ethynyl-2′-deoxyuridine (EdU, Click Chemistry Tools, 3 mg/kg) every day (n = 4-5/group) ...
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bioRxiv - Bioengineering 2022Quote: ... 50 μL antibodies were then modified with UV-cleavable azido-NHS ester (Click Chemistry Tools) at 1:15 molar ratio for 2 h ...
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bioRxiv - Cancer Biology 2020Quote: ... Antibodies were incubated with DBCO-PEG5-NHS Ester linker (Click Chemistry Tools, cat. no. A102P) at a 4:1 molar ratio linker:antibody for 2 h at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... brucei cells (0.7-0.9 × 106 cells/ml) were incubated with 150 µM 5-ethynyl-2’-deoxyuridine (EdU) (Click Chemistry Tools) for 3 hrs before genomic DNA was isolated ...
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bioRxiv - Cell Biology 2023Quote: ... cells are incubated overnight in culture medium with 5 μM of 5-ethynyl 2’-deoxyuridine (EdU, #1149, Click Chemistry Tools) diluted from a 10 mM stock in DMSO ...
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bioRxiv - Bioengineering 2022Quote: ... 50 μL of antibodies were reacted with 10 mM photocleavable azido-NHS ester (Click Chemistry Tools) at 1 ...
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bioRxiv - Bioengineering 2020Quote: ... cytokines were reacted with a commercial 2-nitrobenzyl linker displaying both NHS ester and DBCO substituents (1160-10, Click Chemistry Tools) followed by addition of poly(ethylene glycol ...
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bioRxiv - Biochemistry 2019Quote: ... artificial amino acid-containing base was incubated at room temperature with 150 µM 5,5′-dithiobis-2-nitrobenzoic acid for 10 minutes before chilling on ice and adding 300 µM DBCO-Cy3 (Click Chemistry Tools) and incubating at 4°C overnight ...
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bioRxiv - Microbiology 2022Quote: ... L-AHA-containing proteins were labeled with Cy7 by strain-promoted azide-alkyne click-chemistry employing 1 μM dibenzylcyclooctyne-Cy7 (DBCO-Cy7) (Click Chemistry Tools, USA, Scottsdale). After methanol-chloroform extraction ...
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bioRxiv - Immunology 2020Quote: ... A 500 μL labeling reaction containing 0.5 mg of ovalbumin in 1X BBS and 50 μL of 2 mM mTz-PEG4-NHS in DMSO (0.2 mM final; Click Chemistry Tools, 1069), pH 8.5 was rotated at 4 °C overnight ...
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bioRxiv - Developmental Biology 2022Quote: ... The click reaction was performed for 1 hour in click staining solution (100 mM Tris pH 8.5, 1 mM CuSO4, 2 mM AZDye-488 Azide (Click Chemistry Tools, 1275-1), and 100 mM ascorbic acid) ...
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bioRxiv - Bioengineering 2023Quote: 7-(Diethylamino)-4- (hydroxymethyl)coumarin (DEAC-OH) was previously synthesized in-house (Method S3) and conjugated to 2-azidoacetic acid (Click Chemistry tools, 1081), 3-azidopropionic acid (Synthonix ...
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bioRxiv - Physiology 2023Quote: ... strain-promoted inverse electron-demand Diels-Alder cycloaddition (SPIEDAC) was performed by adding 1-2 μM tetrazine dye (Click Chemistry Tools, Scottsdale, AZ) to the culture medium and incubating it with the cells at 37°C for 30 minutes ...
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bioRxiv - Molecular Biology 2021Quote: The Click reaction was then performed by incubating cells with 100 µL Click buffer (100 µM copper sulfate, 2 mM THPTA, 10 mM fresh sodium ascorbate, 10 µM Cy5 picolyl azide (Click Chemistry Tools 1171-1)) for 1 hour at 37°C in the dark ...
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bioRxiv - Molecular Biology 2022Quote: ... in situ click reactions were performed by incubating cells with 100 µL Click buffer (100 µM copper sulfate, 2 mM THPTA, 10 mM fresh sodium ascorbate, 10 µM Cy3 picolyl azide (Click Chemistry Tools 1178-1)) for 1 hour at 37°C in the dark ...
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bioRxiv - Synthetic Biology 2023Quote: ... Antibodies were then co-incubated with a 20-fold molar excess of NHS ester: PC-biotin-NHS ester (Click Chemistry Tools), Biotin-PEG2-NHS ester (BroadPharma) ...
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bioRxiv - Immunology 2024Quote: ... Successful attachment of azide groups to antibodies was confirmed by a test conjugation with DBCO-AZDye (Click Chemistry Tools/Vector Laboratories) followed by detection of fluor-conjugated antibodies using UltraComp eBeads (ThermoFisher) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Antibodies were functionalized by reacting with a 5-fold molar excess of dibenzocyclooctyne-PEG4-NHS ester (DBCO-PEG4-NHS) (Click Chemistry Tools, Scottsdale, AZ) for 30 minutes at room temperature ...