-
No products found
because this supplier's products are not listed.
Scott Birks, et al.,
bioRxiv - Bioengineering 2024
Quote:
... Sun-2 (Abcam, ab87036), Nesprin-2 (ImmuQuest ...
-
No products found
because this supplier's products are not listed.
Victoria Bryson, et al.,
bioRxiv - Physiology 2023
Quote:
... SUN2 (Millipore MABT880), cGAS (Cell signaling ...
-
No products found
because this supplier's products are not listed.
Sandra Vidak, et al.,
bioRxiv - Cell Biology 2022
Quote:
... MP029-CRY2olig-mCh-SUN2-N-1 or MP029-CRY2olig-mCh-SUN2-N-2 using Lipofectamine LTX with Plus Reagent (Thermo Fisher Scientific) according to manufacturer instructions ...
-
No products found
because this supplier's products are not listed.
Fatima Amanat, et al.,
bioRxiv - Microbiology 2022
Quote:
... proteins from Sino Biologicals (with no trimerization domain) were purchased and used ...
-
No products found
because this supplier's products are not listed.
Sandra Vidak, et al.,
bioRxiv - Cell Biology 2022
Quote:
... and SUN2-N-2 (AA 1-226) fragments by PCR from pcDNA3.1+/C-(K)DY-SUN2 vector (OHu01874,GenScript # NM_001199579.1) and subsequent cloning into MP029-CRY2-mCherry lentiviral vector using the NheI/XbaI restriction sites ...
-
No products found
because this supplier's products are not listed.
Chaogu Zheng, et al.,
bioRxiv - Developmental Biology 2020
Quote:
... were used to express proteins fused to the yeast-activating domain (AD) and -binding domain (BD), respectively ...
-
No products found
because this supplier's products are not listed.
Kelsey A. Haugh, et al.,
bioRxiv - Microbiology 2020
Quote:
... pMIG-Fluc-IRES-mCherry was a gift from Xiaoping Sun (Addgene plasmid # 75020 ...
-
No products found
because this supplier's products are not listed.
K. Polak, et al.,
bioRxiv - Immunology 2020
Quote:
... or S2 domain (SARS-CoV-2 Spike S2, mouse monoclonal antibody [1A9], GTX632604, Genetex), or CilPP (in-house antibody raised in rabbit) ...
-
No products found
because this supplier's products are not listed.
Dean Gilham, et al.,
bioRxiv - Pharmacology and Toxicology 2021
Quote:
... and incubated with recombinant SARS-CoV-2 Spike protein receptor binding domain (RBD) fused to the human IgG1 Fc domain (R&D Systems 10499-CV-100) or control recombinant human IgG1 Fc protein (R&D Systems 10499-CV-100 ...
-
No products found
because this supplier's products are not listed.
Christian L. Egly, et al.,
bioRxiv - Biochemistry 2024
Quote:
... that combines the IgG binding domain of both Protein A and Protein G were used to cross-link anti-Kv11.1 antibodies (Cell Signaling). Stock beads with a concentration of 10 mg/ml were washed four times with 10 volumes of Dulbecco’s Phosphate Buffered Saline (DPBS ...
-
No products found
because this supplier's products are not listed.
Camila H. Coelho, et al.,
bioRxiv - Immunology 2023
Quote:
... biotinylated full-length SARS-CoV-2 Spike protein was purchased from Acro Biosystems and SARS-CoV-2 Spike protein Receptor-Binding Domain (RBD) from BioLegend. SARS-CoV-2 Spike protein Receptor-Binding Domain (RBD ...
-
No products found
because this supplier's products are not listed.
Karen Kar Lye Yee, et al.,
bioRxiv - Neuroscience 2024
Quote:
... and 10 µg/mL synaptotagmin 2 antibody cytoplasmic domain (Synaptic systems). To enhance antibody uptake ...
-
No products found
because this supplier's products are not listed.
Joana T. Lima, et al.,
bioRxiv - Cell Biology 2023
Quote:
... anti-SUN2 (1:500; MABT880; Merck Millipore), anti-GAPDH (1:20000 ...
-
No products found
because this supplier's products are not listed.
Yunsik Kang, et al.,
bioRxiv - Neuroscience 2023
Quote:
... PCR amplified Domain 1 and PCR Domain 2 and HiFi DNA assmbley was performed (NEB). Transformation was performed in DH5α cells (REF) ...
-
No products found
because this supplier's products are not listed.
Kinya Nomura, et al.,
bioRxiv - Plant Biology 2023
Quote:
... and a rabbit LexA binding domain antibody (Clontech), respectively.
-
No products found
because this supplier's products are not listed.
Jonathan M. Schmitz, et al.,
bioRxiv - Biochemistry 2022
Quote:
... constructs containing DNA encoding 8X-His-tagged CHI-domain-containing proteins were transformed into BL21-CodonPlus (DE3)-RIPL Competent Cells (Agilent) for recombinant protein expression ...
-
No products found
because this supplier's products are not listed.
Yuki Yoshida, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... MnCl2 or CaCl2was titrated into a sample cell containing 0.1 mM catalytic domain of the g12777 protein using an iTC200 calorimeter (GE Healthcare).
-
No products found
because this supplier's products are not listed.
Dhirendra Kumar Sharma, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... Antibodies against the T18 (SC-13582) domains of CyaA protein of Bordetella pertussis were procured from Santa Cruz Biotechnology ...
-
No products found
because this supplier's products are not listed.
Ajay Kumar, et al.,
bioRxiv - Immunology 2021
Quote:
... 1μg of each protein (wild type, single domain, and domain deletion mutant) was immobilized onto NC membranes (0.2μ pore size; Bio-Rad). The membranes were kept for drying for 5-10 min at RT ...
-
No products found
because this supplier's products are not listed.
D. N. Clarke, L. Formery, C. J. Lowe,
bioRxiv - Developmental Biology 2024
Quote:
... The samples were then blocked in blocking solution (maleic acid buffer containing 2% Boehringher-Mannheim blocking reagent) and incubated overnight at 4°C in blocking solution containing anti digoxigenin antibody (Roche) diluted at 1:2000 ...
-
No products found
because this supplier's products are not listed.
Seyedehmahsa Moghimi, et al.,
bioRxiv - Microbiology 2022
Quote:
... the FLAG-APEX2 was fused in-frame upstream of the GBF1 truncated after HDS1 domain and containing a BFA-resistant Sec7 domain from ARNO (GARG-1060) in a mammalian expression vector pCI (Promega) generating a plasmid pCI-FLAG-APEX2-GARG- 1060 ...
-
No products found
because this supplier's products are not listed.
M. Verónica López, et al.,
bioRxiv - Immunology 2021
Quote:
... Cells were cultured for 18 h at 5×105 cells per well with 2 μg/ml of a peptide pool consisting mainly of 15-mers (overlapping by 11 amino acids) covering the immunodominant sequence domains of the SARS-CoV-2 Spike protein (PepTivator® SARS-CoV-2 Prot_S; Miltenyi Biotec, Germany). The number of spots was determined using an automatic ELISPOT reader and image analysis software (CTL-ImmunoSpot® S6 Micro Analyzer ...
-
No products found
because this supplier's products are not listed.
Wei Yang, et al.,
bioRxiv - Cell Biology 2024
Quote:
... Proteins were immunoprecipitated with NRBP1/2 antibody (21549-1-AP, Proteintech) or normal rabbit IgG (sc-2027 ...
-
No products found
because this supplier's products are not listed.
Jonathan Pini, et al.,
bioRxiv - Cell Biology 2020
Quote:
The validated Alx1DN (N-terminal portion of protein product containing homeodomain and nuclear localization domains) clones in pCS2+8 were purified via miniprep (Qiagen) alongside a control (C-terminal portion containing transactivation domain) ...
-
No products found
because this supplier's products are not listed.
Bjoern Traenkle, et al.,
bioRxiv - Immunology 2021
Quote:
... VHH domain (Jackson ImmunoResearch) was applied for 15 min ...
-
No products found
because this supplier's products are not listed.
Karlijn B. Rombouts, et al.,
bioRxiv - Cell Biology 2024
Quote:
... PDZ and LIM domain protein 4 (1:1000, NBP1-80833, Novus Biologicals), NUAK family kinase 1 (1:1000 ...
-
No products found
because this supplier's products are not listed.
Christine Chable-Bessia, et al.,
bioRxiv - Microbiology 2021
Quote:
... Incubation with primary antibodies anti-SARS-CoV-2 rabbit membrane (M) protein (Rockland) (1:100 ...
-
No products found
because this supplier's products are not listed.
Sander Barnhoorn, et al.,
bioRxiv - Neuroscience 2023
Quote:
... containing 2 mM Ultraglutamine (Lonza), 1% Nonessential aminoacids (NEAA) ...
-
No products found
because this supplier's products are not listed.
Kailin Yin, et al.,
bioRxiv - Immunology 2023
Quote:
... containing 2 mM EDTA (Corning) and 25 µM cisplatin (Sigma) ...
-
No products found
because this supplier's products are not listed.
Hisako Takigawa-Imamura, et al.,
bioRxiv - Developmental Biology 2024
Quote:
... containing recombinant human FGF10 protein (PeproTech) was overlaid on explants and allowed to solidify for 15 min at 37°C ...
-
No products found
because this supplier's products are not listed.
Sangderk Lee, et al.,
bioRxiv - Neuroscience 2023
Quote:
... containing 2% FBS (VWR #97068-085) and 1% pen/strep (Gibco #15140-122 ...
-
No products found
because this supplier's products are not listed.
Ugo Sardo, et al.,
bioRxiv - Physiology 2023
Quote:
Mouse FGL1 cDNA sequences (full length, N-terminal domain, globular domain) were cloned into pFUSEN-hG2Fc plasmid (InvivoGen) with the following modifications ...
-
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...
Cat# LS004174,
100 mg, $42.00
Ask
Jenny A.F. Vermeer, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... containing Collagenase Type 2 (Worthington Biochemical Corporation) and DNAse I (ThermoFisher Scientific ...
-
No products found
because this supplier's products are not listed.
Shady Younis, et al.,
bioRxiv - Cell Biology 2022
Quote:
... anti-ZC3H11A or anti-THOC2 antibodies in Protein LoBind 2-ml tubes (Eppendorf). Thereafter ...
-
No products found
because this supplier's products are not listed.
Junchao Wei, et al.,
bioRxiv - Bioengineering 2022
Quote:
Spectral-domain OCT (Leica, Chicago, IL, USA), with a broadband superluminescent diode light source (Superlum ...
-
No products found
because this supplier's products are not listed.
G.A. Wildenberg, et al.,
bioRxiv - Neuroscience 2020
Quote:
... followed by fixative containing 2% paraformaldehyde (EMS), 2.5% glutaraldehyde (EMS ...
-
No products found
because this supplier's products are not listed.
Juan A. Perez-Bermejo, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... Purified 53BP1 Tudor domain was labeled at exposed primary amine groups with NHS-biotin using ChromaLINK NHS-Biotin protein labeling kit (Vector Laboratories). 1 equivalent of chromalink biotin was incubated with the 53BP1 Tudor domain for 2 h and buffer exchanged into fresh PBS ...
-
No products found
because this supplier's products are not listed.
Sumit Sen Santara, et al.,
bioRxiv - Immunology 2021
Quote:
... control antibody (LTF-2, BioXCell), rabbit anti-human CRT (ab2907 ...
-
No products found
because this supplier's products are not listed.
Gaurav V. Sarode, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... Coupled antibody-Dynabeads were prepared by adding Dynabeads protein A/G and 2 μg antibody [H3K9ac (Active Motif) or H3K27ac (Abcam)] to 100 μl ice-cold dilution buffer and rotating the tubes end-over-end at 4°C for 1 hour ...
-
No products found
because this supplier's products are not listed.
Shahbaz Ahmed, et al.,
bioRxiv - Biophysics 2021
Quote:
... containing chicken anti-HA antibodies from Bethyl labs (1˸600 dilution ...
-
No products found
because this supplier's products are not listed.
R. Ryley Parrish, et al.,
bioRxiv - Neuroscience 2023
Quote:
... with mice containing a floxed STOP cassette in front of an eNpHR3.0/EYFP domain (Jackson Laboratory, Stock 014539 ...
-
No products found
because this supplier's products are not listed.
Bryce W. Duncan, et al.,
bioRxiv - Neuroscience 2021
Quote:
... coiled-coil containing protein kinase (ROCK1/2) Y27632 (Tocris, Inc.) at 10 µM in PBS for 1-2 hr ...
-
No products found
because this supplier's products are not listed.
Chi-Hong Wu, et al.,
bioRxiv - Neuroscience 2021
Quote:
... the protein-antibody-bead mixtures were resuspended directly in the SDS-containing loading buffer (30 µl, LI-COR) and the Shank3 proteins were eluted into the buffer by heating at 70°C for 10 minutes ...
-
No products found
because this supplier's products are not listed.
Murat Artan, et al.,
bioRxiv - Biochemistry 2021
Quote:
Polyacrylamide gel slices (1-2 mm) containing the purified proteins were prepared for mass spectrometric analysis using the Janus liquid handling system (PerkinElmer, UK). Briefly ...
-
No products found
because this supplier's products are not listed.
Tatsuki Nakagawa, et al.,
bioRxiv - Neuroscience 2024
Quote:
... 6’- diamidino-2-phenylindole (DAPI)-containing mounting solution (SouthernBiotech), and a coverslip was applied ...
-
No products found
because this supplier's products are not listed.
Emma Carley, et al.,
bioRxiv - Cell Biology 2020
Quote:
... 50,000 nuclei were purified from WT or Sun dKO cells and DNA was tagmented using the Nextera Library Prep Kit (Illumina). Tagmented DNA was purified using the Qiagen MinElute PCR Purification kit ...
-
No products found
because this supplier's products are not listed.
SS Parker, et al.,
bioRxiv - Cell Biology 2021
Quote:
... containing 2% NeuroCult SM1 supplement (StemCell Technologies, 05711), 1% L-glutamine (Corning ...
-
No products found
because this supplier's products are not listed.
Logan H. Hodgskiss, et al.,
bioRxiv - Microbiology 2022
Quote:
Supernatant containing proteins and membrane were ultracentrifuged at 200,000xg (Beckman Coulter Ultracentrifuge ...
-
No products found
because this supplier's products are not listed.
Steven Z. Chou, Thomas D. Pollard,
bioRxiv - Biochemistry 2023
Quote:
... the same proteins were applied to holey grids with a 2 nm carbon film (Quantifoil Cu 2/2 200 mesh from EMS). Samples were frozen after a total of 145 min on the grids without carbon film and 150 min on the grids with carbon film ...
-
No products found
because this supplier's products are not listed.
Matthieu Colpaert, et al.,
bioRxiv - Microbiology 2020
Quote:
The TreS activity domain of TreS-Mak protein was monitored following the conversion of trehalose into maltose and glucose using Epoch spectrophotometer (Biotek). 15µL of reaction sample were incubated 30 min at 58°C with 30 µL of 100 mM sodium citrate pH 4.6 containing whether 0.4 U of amyloglucosidase from Aspergillus niger (Megazyme ...