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Citations for Electron Microscopy Sciences :
1 - 50 of 894 citations for SUN domain containing protein 2 SUN2 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... followed by fixative containing 2% paraformaldehyde (EMS), 2.5% glutaraldehyde (EMS ...
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bioRxiv - Neuroscience 2021Quote: ... followed by 20 ml of fixative containing 2% paraformaldehyde (EMS), 2.5% glutaraldehyde (EMS ...
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bioRxiv - Neuroscience 2021Quote: ... followed by 20 ml of fixative containing 2% paraformaldehyde (EMS), 2.5% glutaraldehyde (EMS ...
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bioRxiv - Neuroscience 2021Quote: ... A small piece (∼2 × 2 mm) containing V1 was cut out and prepared for EM by staining sequentially with 2% osmium tetroxide (EMS) in Sodium Cacodylate buffer ...
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bioRxiv - Cell Biology 2021Quote: ... 1.5 in needle containing 2 mL 4% PFA (Electron Microscopy Sciences) diluted in stabilization buffer ...
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bioRxiv - Cell Biology 2023Quote: ... and fixative II containing 2% osmium tetroxide (Electron Microscopy Sciences, 19132) both prepared in anhydrous acetone ...
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bioRxiv - Biochemistry 2023Quote: ... the same proteins were applied to holey grids with a 2 nm carbon film (Quantifoil Cu 2/2 200 mesh from EMS). Samples were frozen after a total of 145 min on the grids without carbon film and 150 min on the grids with carbon film ...
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bioRxiv - Cell Biology 2020Quote: ... samples were substituted in a medium of acetone containing 2% osmium tetroxide (EMS), 0.2% uranyl acetate (Merck ...
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bioRxiv - Neuroscience 2023Quote: ... the interscutularis muscle was immersed with a solution containing 2% glutaraldehyde (Glut., EMS, USA) and 2% paraformaldehyde (PFA ...
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bioRxiv - Neuroscience 2023Quote: ... and then incubated in a solution containing 2 w/v% OsO4 (Electron Microscopy Sciences), 1.5% w/v potassium ferrocyanide (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... It was then incubated in a solution containing 2% osmium tetroxide (19150, Electron Microscopy Sciences) and 1.5% potassium ferricyanide (20150 ...
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bioRxiv - Cell Biology 2022Quote: ... It was then incubated in a solution containing 2% osmium tetroxide (19150, Electron Microscopy Sciences) and 1.5% potassium ferricyanide (20150 ...
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bioRxiv - Neuroscience 2021Quote: ... the slice was placed in a fixative containing 2% formaldehyde (Electron Microscopy Sciences 157-8) and 2 % glutaraldehyde (Electron Microscopy Sciences 16220 ...
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bioRxiv - Cell Biology 2020Quote: ... The coverslips were then moved immediately into unroofing buffer containing 2% glutaraldehyde (Electron Microscopy Sciences, 16019). With syringe ...
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bioRxiv - Neuroscience 2021Quote: ... sapphire disks were placed into 12-well plate containing 1 mL of 2% glutaraldehyde (EMS #16530) and 1 mM CaCl2 (Sigma-Aldrich # 63535 ...
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bioRxiv - Genetics 2020Quote: ... sections were mounted with Fluoromount-G containing 4′,6-diamidino-2-phenylindole (DAPI) (Electron Microscopy Sciences). Staining was visualized and imaged using a Nikon 80i microscope with a Nikon DS-Fi1 camera or a Nikon Eclipse TiE attached to an A1R-SHR confocal ...
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bioRxiv - Neuroscience 2024Quote: ... samples were incubated in a 2% OsO4 in 0.15 M cacodylate buffer solution containing 2 mM calcium chloride (19152, Electron Microscopy Sciences, Hatfield, PA, USA) for 2 hours in the dark ...
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bioRxiv - Neuroscience 2024Quote: ... and 0.15M cacodylate buffer containing 2 mM calcium chloride (16537-15, Electron Microscopy Sciences, Hatfield, PA, USA) overnight at 4° C ...
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bioRxiv - Bioengineering 2023Quote: ... 4 µl of AAV9 capsids containing GFP or Cas9 cargo was deposited on 2/2 400 mesh C-flat grids (Electron Microscopy Sciences, Hatfield, PA, #CF224C-50), which were previously glow discharged at 15 mA for 15 s on PELCO easyGLOW instrument ...
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bioRxiv - Microbiology 2024Quote: ... 10 μL of parasite-containing media from each flask was added to 90 μL of 2% PFA (EMS) to fix and dilute the parasites at a 1:10 ratio in a 96-well flat bottom plate ...
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bioRxiv - Synthetic Biology 2022Quote: ... or Ang1 at 100 nM of F-domain concentration for 15 min before fixing with 4% PFA (EMS, 15710) for 15 min ...
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bioRxiv - Cell Biology 2022Quote: ... before being fixed by perfusion using 0.15M cacodylate buffer (pH 7.4) containing 2% formaldehyde and 2.5% glutaraldehyde (Electron Microscopy Sciences) as well as 2mM CaCl2 at 35°C for 5 minutes ...
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Comparative membrane proteomics reveals diverse cell regulators concentrated at the nuclear envelopebioRxiv - Cell Biology 2023Quote: ... cells were rinsed with PBS containing calcium and magnesium and fixed using 2% paraformaldehyde (PFA) (Electron Microscopy Sciences # 15710) in PBS for 20 min ...
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bioRxiv - Neuroscience 2023Quote: ... samples were washed 3–4 times 10 min each with fresh acetone chilled to −90°C and left at the same temperature for 6 hours in acetone containing 2% osmium (Science services (EMS)) and 0.2% uranyl acetate (Serva) ...
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bioRxiv - Physiology 2024Quote: ... pH 7.4 for 2-weeks followed by incubation in a PBS solution containing 1% osmium tetroxide (Electron Microscopy Sciences, 19170) and 2.5% potassium dichromate (Sigma-Aldrich ...
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bioRxiv - Immunology 2024Quote: ... and cells were fixed and stained with solution containing final concentrations of 2% paraformaldehyde (PFA; Electron Microscopy Sciences, #15713-S) in DPBS ...
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bioRxiv - Cell Biology 2022Quote: ... 2-3 μl of protein was applied to glow-discharged carbon-coated copper grids (EMS; CF-400-Cu) and incubated for 45 s at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... pH 7.4 buffer for P116 without the N-terminal domain (246–818)) was applied to a 45 s glow- discharged R1.2/1.3 C-flat grid (Electron Microscopy Science, Hatfield, USA), and plunge-frozen in liquid ethane (Vitrobot Mark IV ...
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bioRxiv - Cell Biology 2021Quote: Osteoclasts plated on bone slices for 3 days were unroofed as described above and fixed for 10 min in a 0.2M sodium cacodylate buffer (pH 7.4) containing 2% paraformaldehyde (Electron Microscopy Sciences 15710) and 2.5% glutaraldehyde (Electron Microscopy Sciences 16220) ...
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bioRxiv - Plant Biology 2021Quote: ... The roots of 7 to 10 day old seedlings were collected and fixed for 30 min with 2-(N-morpholino) ethane sulfonic acid (MES) buffer solution pH 5.7 containing 4% paraformaldehyde (32% methanol-free solution; Electron microscopy Sciences). pH was adjusted to 5.7 with KOH solution.
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bioRxiv - Neuroscience 2021Quote: ... Copper grids containing thin sections were post-stained for 20 min in 2% aqueous uranyl acetate (Electron Microscopy Sciences; Cat #22400) and for 5 min in Reynolds lead citrate (Reynolds ...
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bioRxiv - Physiology 2021Quote: The mouse liver was cut into 2-3 mm3 cubes and immediately immersed in a fixation solution containing 2.5% glutaraldehyde (Electron Microscopy Science) in 0.1 M cacodylate buffer pH 7.4 for 1 hour at room temperature and then overnight at 4°C ...
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bioRxiv - Immunology 2024Quote: ... cells were washed with PBS twice followed by fixation and permeablization in 1 ml PBS containing 2% PFA (Electron Microscopy Sciences) and 0.1% saponin (Sigma–Aldrich ...
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bioRxiv - Physiology 2020Quote: 1-2 tracheal rings were removed post-mortem and embedded in Peel-A-Way embedding molds containing Tissue-Tek OCT (Electron Microscopy Sciences). Molds were placed in a container filled with dry ice until frozen ...
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bioRxiv - Neuroscience 2020Quote: ... Rbp4-Cre;Ai14;Snap25+/+ and Rbp4-Cre;Ai14;Snap25fl/fl brains at P18 were perfused with 0.1 M phosphate buffer containing 2% PFA (Electron Microscopy Sciences, 15714) and 2.5% glutaraldehyde (Electron Microscopy Sciences ...
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bioRxiv - Pathology 2021Quote: Liver tissue samples were fixed in 0.1 M cacodylate-buffered Karnovsky fixative containing 2.5% glutaraldehyde and 2% paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA) overnight at room temperature with a subsequent post-fixation in 1% osmium tetroxide (Electron Microscopy Sciences) ...
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bioRxiv - Bioengineering 2022Quote: ... the cholangiocyte organoids were fixed with TEM fixative containing 2% paraformaldehyde/2.5 glutaraldehyde in 100mM cacodylate buffer (Electron Microscopy Sciences, Hartfield, PA) for 2 hours at room temperature ...
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bioRxiv - Biophysics 2023Quote: ... containing 4% paraformaldehyde (EMS) and 0.1% glutaraldehyde in PBS ...
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bioRxiv - Biophysics 2024Quote: ... the cells were fixed for 10 minutes in PEM buffer (80 mM PIPES, 5 mM EGTA, 2 mM MgCl2) containing 4% formaldehyde (FA) (32% sol. in glass ampoules, Electron Microscopy Sciences, #50980494) with 4% sucrose (Sigma ...
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bioRxiv - Cell Biology 2024Quote: Primary neurons or astrocytes cultured on glass coverslips at the same plating density as was used for live cell imaging experiments were fixed for 10 minutes at 37°C/5% CO2 in a solution containing 2% paraformaldehyde (PFA; Electron Microscopy Sciences 15713) and 0.05% glutaraldehyde in PHEM buffer (EMS ...
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bioRxiv - Neuroscience 2021Quote: ... containing 1% OsO4 (EMS, USA) and 1.5% C6FeK4N6 (EMS ...
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bioRxiv - Cell Biology 2023Quote: ... containing 0.1% tanic acid (EMS),1% glutaraldehyde (EMS ...
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bioRxiv - Neuroscience 2024Quote: ... containing 2.5% glutaraldehyde (EMS, #18220), 2% freshly made paraformaldehyde (EMS ...
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bioRxiv - Molecular Biology 2021Quote: 4 µL of 0.05 mg/mL SARS-CoV-2 spike protein was applied onto a freshly glow discharged (1 min at 10 mA) CF300-Cu-50 grid (Electron Microscopy Sciences), incubated for 1 min ...
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bioRxiv - Biochemistry 2022Quote: ... a 3-μl aliquot of the protein sample was applied to a glow-discharged Quantifoil holey carbon grid (Au 300 2/1 mesh) (Electron Microscopy Sciences), blotted for 2 s ...
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bioRxiv - Pathology 2022Quote: ... Slides were incubated at RT for an hour in secondary antibody and mounted with Fluoro-Gel II containing DAPI (Electron Microscopy Sciences, 17985). Slides were viewed using a Zeiss Axio Imager.A2 Microscope and images were taken using Axiocam 105 color (chromogenic detection ...
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bioRxiv - Developmental Biology 2021Quote: ... containing O.C.T compound (EMS #62550-0) and flash frozen below a cold alcohol slurry ...
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bioRxiv - Neuroscience 2023Quote: ... containing fixative (1% glutaraldehyde (EMS 16530), 0.1% Tannic acid (Sigma 403040-100G ...
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bioRxiv - Cell Biology 2024Quote: ... containing 4% (v/v) paraformaldehyde (EMS), 0.2% (v/v ...
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bioRxiv - Biochemistry 2024Quote: CryoEM grids were prepared by applying 3.5 μL of 0.6 mg/mL NX11Q21.5-DNA48 in TBS with 2.5% glycerol to glow-discharged 400 mesh C-flat grids (CF-2/2-4C, 2 μm holes, 2 μm spacing; Electron Microscopy Sciences). The grids were blotted with a blot force of 0 for 6.5 seconds at 100% humidity and 20 °C ...