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Citations for SouthernBiotech :
1 - 50 of 698 citations for SUN domain containing protein 2 SUN2 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... 6’- diamidino-2-phenylindole (DAPI)-containing mounting solution (SouthernBiotech), and a coverslip was applied ...
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bioRxiv - Cell Biology 2020Quote: ... localization of antibody labeling was detected after a 1 hr incubation with secondary antibody diluted in PBS containing 2% normal goat serum and mounting under a coverslip with DAPI Fluoromount-G (Southern Biotech). PPCs were visualized by fluorescent microscopy and their numbers were assessed qualitatively by visual examination ...
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bioRxiv - Immunology 2022Quote: ... IgG bound to the rSARS-CoV-2 S protein was detected using a horseradish peroxidase (HRP)-conjugated goat anti-human IgG antibody (Southern Biotech) incubated for 1 hour ± 10 minutes at 24°C ± 2°C ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... and mounted with 4’,6-diamidino-2-phenylindole (DAPI)-containing Fluoromount medium (Southern Biotech).
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and mounted with 4’,6-diamidino-2-phenylindole (DAPI) containing Fluormount-GTM (SouthernBiotech, Birmingham, AL) for nuclear staining ...
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bioRxiv - Physiology 2020Quote: ... and mounted with 4’,6-diamidino-2-phenylindole (DAPI) containing Fluormount-GTM (SouthernBiotech, Birmingham, AL) for nuclear staining ...
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bioRxiv - Neuroscience 2023Quote: ... 6-diamidino-2-phenylindole (DAPI)-containing mounting media (DAPI Fluoromount-G, 0100-20, Southern Biotech).
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bioRxiv - Immunology 2023Quote: ... Coverslips were mounted with Fluoromount G containing 4′,6′-diamidino-2 phenyleindole (SouthernBiotech, Birmingham, UK) and cells were observed by direct immunofluorescence ...
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bioRxiv - Cell Biology 2024Quote: ... the sections were stained with 4’,6-diamidino-2-phenylindole (DAPI) containing Fluormount-G (SouthernBiotech, Birmingham, AL) to visualize nuclei ...
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bioRxiv - Immunology 2024Quote: ... Antibody binding was detected using PE-conjugated goat-anti-mouse IgG secondary antibody (2 μg/mL, Southern Biotech). Results were acquired on a Bio-Plex 200 system (Bio-Rad ...
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bioRxiv - Immunology 2023Quote: Biotinylated Goat F(ab)2 anti-human isotype antibodies (anti-human IgM, SouthernBiotech, Cat # 2022-01 ...
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bioRxiv - Neuroscience 2023Quote: ... then mounted in 10 μl of 4,6-diamidino-2-phenylindole dihydrochloride (DAPI)-containing Fluoromount G (Southern Biotech, #0100-20). Coverslips were analyzed by microscopy using a Leica confocal microscope (Leica TCS SPE laser at 25-50% power ...
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bioRxiv - Immunology 2024Quote: ... goat F(ab′)2 anti-human kappa FITC and goat F(ab′)2 anti-human lambda FITC (SouthernBiotech; to detect antibody expression), and PI (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: ... was coated with hPD-L1-His protein and anti-PD-L1 antibody and anti-mouse IgG specific HRP conjugated secondary antibodies (SouthernBiotech, Birmingham, AL, USA) were added ...
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bioRxiv - Immunology 2021Quote: ... cells were resuspended in calcium-containing HBSS and incubated at room temperature for 5 minutes before activation by anti-IgM F(ab′)2 (Southern Biotech) or VLPs ...
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bioRxiv - Microbiology 2022Quote: ... then incubated in 0.1% TBST containing 1:4000 goat anti-rabbit antibody conjugated to HRP for 1 h at RT (SouthernBiotech).
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bioRxiv - Physiology 2020Quote: ... The sections were processed for immunostaining of Pan-CK (--) as described above with 4’,6-diamidino-2-phenylindole (DAPI) containing Fluormount-G (SouthernBiotech, Birmingham, AL) to visualize nuclei.
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bioRxiv - Cell Biology 2020Quote: ... containing FluoromountG (Southern Biotech). Images were acquired using a Delta Vision microscope as described (Ovciarikova et al. ...
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bioRxiv - Neuroscience 2020Quote: ... slides were incubated at room temperature for 2 hours in secondary antibodies diluted in the blocking solution (at 1:500 for Alexa-coupled antibodies (Life Sciences)) and then mounted using Hoechst-containing Fluoromount-G (Southern Biotech).
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bioRxiv - Immunology 2021Quote: ... buffer (PBS with 0.1% bovine serum albumin and 0.1% sodium azide) containing the PE-labeled tetrameric pBF2*1501 complex and fluorescein isothiocyanate (FITC)-labeled anti-CD8 monoclonal antibody (SouthernBiotech, USA) for 30 min at 4°C ...
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bioRxiv - Immunology 2023Quote: ... Sections were washed in PBS before incubation with secondary antibodies or labelled streptavidin (Supplementary Table 4) for 1hr at room temperature followed by mounting with DAPI containing fluoromount (Southern Biotech). Small to medium sized airways in lung sections were captured by an investigator blind to sample identity with an EVOS FL imaging system (Thermo) ...
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bioRxiv - Biophysics 2023Quote: ... at a 1:250 dilution and protein was detected with a HRP-conjugated goat anti-mouse antibody (Southern Biotech) using a 1:5000 dilution ...
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bioRxiv - Microbiology 2020Quote: ... Bound antibodies were detected with horseradish peroxidase (HRP)-conjugated goat F(ab’)2 anti-human kappa (Southern Biotech) in 1% BSA in PBS-T and Tetramethylbenzidine (TMB ...
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bioRxiv - Immunology 2020Quote: ... CMV glycoprotein–specific antibody binding was detected with phycoerythrin-conjugated goat anti-human IgG (2 μg/mL, Southern Biotech). Beads were washed and acquired on a Bio-Plex 200 instrument (Bio-Rad) ...
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bioRxiv - Immunology 2022Quote: ... ELISA plates were coated with 1 μg/ml recombinant SARS-CoV-2 S protein (43) and serially diluted serum samples were added and detected via anti-monkey IgG-HRP (Southern Biotech, Birmingham, AL). Plates were developed using a TMB substrate (source ...
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bioRxiv - Neuroscience 2024Quote: ... with Fluoromount-G containing DAPI (SouthernBiotech). Slides were kept in the dark at 4°C until imaging on a Nikon A1 confocal microscope.
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bioRxiv - Immunology 2021Quote: ... Sections were washed in PBS before incubation with secondary antibodies (Table 3) for 1 hr at room temperature followed by mounting with DAPI containing fluoromount (Southern Biotech, Birmingham, AL, USA). Images were captured with an EVOS FL imaging system (ThermoFisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... Pellet was then resuspended in 100 µL ice-cold PBS containing 4 µg of FITC-coupled goat anti-mouse IgA antibody (SouthernBiotech, Imtec Diagnostic, #1040-02). The ratio of 4 µg of the respective antibody to stain 109 bacteria was previously evaluated to be the maximum amount of antibody that can be used without resulting in unspecific staining of non-IgA coated bacteria by using fecal samples from Rag1−/− mice as non IgA-coated negative controls ...
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bioRxiv - Cell Biology 2023Quote: ... Surface-bound sLAP was detected with anti-human PE antibodies that bound directly to the Fc-IgG part of the chimeric sLAP proteins (SouthernBiotech, USA). PE signals were analyzed with FlowJo software (BD Biosciences ...
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bioRxiv - Immunology 2023Quote: Biotinylated Goat F(ab)2 anti-human isotype antibodies (anti-human IgM, SouthernBiotech, Cat # 2022-01; anti-human IgA, SouthernBiotech, Cat # 2052-01 ...
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bioRxiv - Bioengineering 2024Quote: ... the slides were incubated with secondary antibodies for 2 hours at room temperature and then finally sealed with DAPI Fluoromount-G (0100-20, SouthernBiotech).
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bioRxiv - Cell Biology 2024Quote: ... the slides were incubated with secondary antibodies for 2 hours at room temperature and then finally sealed with DAPI Fluoromount-G (0100-20, SouthernBiotech). For hematoxylin and eosin staining ...
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bioRxiv - Microbiology 2024Quote: ... Cells were incubated cell side down in 100 µL of Stellaris RNA FISH Hybridization Buffer (BioSearch) containing M1 vRNA probes (1/100) and the primary antibodies of interest (NP, Southern Biotech, 10770-01, 1/100) and incubated in a humid chamber at 37°C in the dark for 16 h ...
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bioRxiv - Cell Biology 2023Quote: ... The presence of the sLAP proteins was verified by western blotting according to standard protocols and by staining with anti-human HRP antibodies (Southern Biotech, USA). Concentration of sLAP proteins was quantified by dot blotting and using purified human Fc-IgG (SigmaAldrich ...
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bioRxiv - Microbiology 2020Quote: ... Antibody binding was detected using directly labeled IgG or Alexa Fluor647-conjugated goat F(ab’)2 anti-human kappa (Southern Biotech). Samples were measured using flow cytometry (BD FACSVerse ...
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bioRxiv - Immunology 2020Quote: ... plates were coated with 2 µg/mL of goat anti-human kappa or goat anti-human lambda antibodies cross-absorbed against mouse IgG (Southern Biotech) at 4°C overnight ...
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bioRxiv - Microbiology 2021Quote: ... Fab fragments were detected with Alexa Fluor 647 conjugated goat-anti-human-kappa F(ab’)2 antibody (Southern Biotech, 1:500). After washing ...
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bioRxiv - Neuroscience 2024Quote: ... covered by mounting medium containing Dapi (SouthernBiotech), and coverslipped ...
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bioRxiv - Microbiology 2020Quote: ... the wells were incubated for 2 h at 37°C with HRP-mouse anti-human IgG antibody (dilution 1:2,000) (Southern Biotech, Birmingham, AL) followed by the peroxidase substrate ...
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bioRxiv - Neuroscience 2022Quote: ... then mounted with DAPI-containing Fluoromount medium (SouthernBiotech). Tissue was excited and photographed using Zeiss LSM 800 confocal microscopy and analyzed with ImageJ.
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bioRxiv - Microbiology 2021Quote: ... samples were further statically incubated for 1 h at 4°C with Alexa Fluor 647 conjugated goat-anti-human-kappa F(ab’)2 antibody (Southern Biotech, 1:300) and 6 μM Syto9 (Live/Dead BacLight Bacterial Viability Kit ...
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bioRxiv - Immunology 2024Quote: ... coated overnight at 4°C (100 μl per well) with 2 μg/ml Igκ antibodies (Goat Anti-Human kappa-UNLB, SouthernBiotech, ref 2060-01) in PBS ...
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bioRxiv - Immunology 2024Quote: Fifty microliters of appropriately diluted serum samples were added to 96-well plates precoated with anti-mouse Igκ and Igλ antibodies (2 mg/ml; Southern Biotech, Birmingham, AL) in 0.1 M carbonate buffer (pH 9.0) ...
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bioRxiv - Neuroscience 2024Quote: ... and Fluoromount-G anti-fading medium containing DAPI (SouthernBiotech).
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bioRxiv - Neuroscience 2020Quote: ... coronal sections containing V1 were mounted using Fluoromount-G (Southern Biotech). Co-labeling of tdTomato and anti-cFos was quantified using Image J software in 6 sections containing V1 from each mouse by a scorer blinded to animal condition ...
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bioRxiv - Neuroscience 2021Quote: ... then mounted the sections with DAPI-containing fluoromount (SouthernBiotech, 0100-20). We imaged slices using a fluorescent microscope (Keyence BZ-X800 ...
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bioRxiv - Neuroscience 2020Quote: ... mounted with DAPI-containing Fluoromount-G (Southern Biotech, Birmingham, AL, USA), and covered with cover slips.
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bioRxiv - Neuroscience 2021Quote: ... Slides were mounted with DAPI containing Fluoromount-G (SouthernBiotech, 0100-20).
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bioRxiv - Immunology 2021Quote: ... All slides were coverslipped with Fluoromount-G containing DAPI (Southern Biotech) and imaged on a Zeiss Axioscope A1 compound microscope.
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bioRxiv - Developmental Biology 2022Quote: ... The slides were coverslipped with Fluoromount G containing DAPI (Southern Biotech) and cured overnight before imaging ...