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No products found
because this supplier's products are not listed.
Tridib Ganguly, et al.,
bioRxiv - Microbiology 2022
Quote:
... Recombinant His-tagged was ZccR overexpressed by addition of 0.2 mM isopropyl-β-D-1- thiogalactopyranoside (IPTG) (Teknova) and the culture was incubated for additional 3 h ...
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No products found
because this supplier's products are not listed.
Elena Arutyunova, et al.,
bioRxiv - Biophysics 2021
Quote:
... The fusion protein was subsequently digested with His-tagged SUMO protease (McLab, South San Francisco, CA) at 4 °C for 1–2 h to remove the SUMO tag and the resulting cleavage mixture was then passed through Ni-NTA resin column ...
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No products found
because this supplier's products are not listed.
Muhammad Rizwan, et al.,
bioRxiv - Bioengineering 2022
Quote:
... Recombinant protein G (Cedarlane Labs Inc). All solvents were purchased from Caledon ...
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Recombinant Antigen
Cat# REC31705-100,
100µg USD $341.0
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Kei Haga, et al.,
bioRxiv - Microbiology 2021
Quote:
... and recombinant SARS-CoV S1 subunits-His tag (The Native Antigen Company, UK) diluted in phosphate-buffered saline (PBS ...
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No products found
because this supplier's products are not listed.
Moumita Sarkar, et al.,
bioRxiv - Developmental Biology 2021
Quote:
... pCDNA-T292A/S294A and pCDNA-Cnot3 Δ651-751 constructs were in vitro transcribed/translated as described above and the resultant V5 tagged proteins were purified using V5-tagged protein purification kit ver.2 (MBL International, USA). Purified proteins were used as substrate and incubated with 80 ng of GST-Aurora B (ProQuinase ...
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WB, IHC,ELISA
Cat# A5436, SKU# A5436-100ul,
100ul, $157.00
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Xuxiao He, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... and Nickel Magnetic Beads for His tag protein purification (Bimake, B23602) according to the manufacturer’s protocol ...
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No products found
because this supplier's products are not listed.
Nils Stührwohldt, et al.,
bioRxiv - Plant Biology 2020
Quote:
... His6-tagged proteins by a monoclonal mouse α–His6 antibody from Dianova (Hamburg, Germany), and Flag-tagged proteins by α–Flag antibody directly coupled to horseradish peroxidase (Taufkirchen ...
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No products found
because this supplier's products are not listed.
Lior Tal, et al.,
bioRxiv - Plant Biology 2023
Quote:
... Arabidopsis KAI2 protein was expressed as a 6× His-SUMO fusion protein using the expression vector pSUMO (LifeSensors). His-SUMO-KAI2 was isolated from by Ni-NTA resin and the eluted His-SUMO KAI2 was further separated by anion-exchange ...
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No products found
because this supplier's products are not listed.
Tomofumi Nakamura, et al.,
bioRxiv - Microbiology 2019
Quote:
Recombinant INHis proteins were examined with a TSKgel-SWXLG3000 column (TOSOH cor., Japan) at 1.0 ml/min in running buffer (20 mM HEPES pH 7.4 ...
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No products found
because this supplier's products are not listed.
Izumi Kurata-Sato, et al.,
bioRxiv - Immunology 2023
Quote:
... biotinylated NP-BSA (Biosearch Technologies) was incubated with APC-conjugated streptavidin (BioLegend ...
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No products found
because this supplier's products are not listed.
Arik Davidyan, Keith Baar, Sue C. Bodine,
bioRxiv - Physiology 2020
Quote:
... The chymotrypsin (β5)-like activities were assayed using 10μg of protein and the fluorescently tagged substrate SUC-LLVY-AMC (Bachem). Both assays were carried out in a total volume of 100μl ...
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No products found
because this supplier's products are not listed.
Charles A Berdan, et al.,
bioRxiv - Cancer Biology 2019
Quote:
... and rotated at 4° C for 2 h before washing 3 × with 500 μL PBS and subsequent elution of FLAG-tagged proteins with 100 μL of 250 ng/μL 3 × FLAG peptide (APExBIO) in PBS ...
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Cat# HY-P70086-50 μg,
50 μg, USD $300.0
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François Serra, et al.,
bioRxiv - Genomics 2023
Quote:
... Degradation of the AID2-tagged RAD21 protein was induced by addition of 1µM of 5-Ph-IAA (MedChemExpress #HY-134653) dissolved in DMSO for at least 6h prior to further experimental procedure ...
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No products found
because this supplier's products are not listed.
Xiaoling Qiang, et al.,
bioRxiv - Immunology 2020
Quote:
... Recombinant proteins were tested for LPS content by the chromogenic Limulus amebocyte lysate assay (Endochrome; Charles River), and the endotoxin content was less than 0.01 U per microgram of recombinant proteins.
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No products found
because this supplier's products are not listed.
Amanda L. Photenhauer, et al.,
bioRxiv - Molecular Biology 2022
Quote:
Custom α-Sas6T antiserum was generated by rabbit immunization with purified recombinant Sas6T protein (Lampire Biological Laboratories). The resulting antiserum was used for western blotting and cell staining ...
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No products found
because this supplier's products are not listed.
Jing Zhao, et al.,
bioRxiv - Plant Biology 2023
Quote:
... His-tag antibody (Tiangen, Beijing, China) and GST antibody (Tiangen).
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No products found
because this supplier's products are not listed.
Christina James, et al.,
bioRxiv - Cell Biology 2023
Quote:
Synthetic biotinylated peptides were purchased from ProteoGenix, France ...
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No products found
because this supplier's products are not listed.
Gina Partipilo, et al.,
bioRxiv - Bioengineering 2021
Quote:
... Bovine serum albumin alkyne (Click Chemistry Tools), sodium DL-lactate (NaC3H5O3 ...
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No products found
because this supplier's products are not listed.
Anvita Bhargava, et al.,
bioRxiv - Microbiology 2020
Quote:
... 0.2% (w/v) bovine serum albumin (BSA) (Euromedex), 0.05% (w/v ...
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No products found
because this supplier's products are not listed.
Vadim Fedyuk, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... Biotinylated antibodies were incubated on surface in IMB2 [10 mM MES pH 6.5 (Boston Bioproducts Inc, NC9904354), 60 mM KCL ...
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No products found
because this supplier's products are not listed.
Benjamin M. Akiyama, et al.,
bioRxiv - Microbiology 2020
Quote:
Cy3-labeled purified RNAs were annealed to biotinylated Cy5-labeled DNA oligos and immobilized on PEGylated cover slides (Ted Pella, Inc.). PEGylated cover slides were prepared as described (Selvin and Ha ...
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No products found
because this supplier's products are not listed.
F Abou Azar, et al.,
bioRxiv - Biochemistry 2022
Quote:
... were isolated from wildtype mice or transgenic mice over-expressing a TAP-tagged 14-3-3ζ that were fed a low-fat (LFD) or 60% high-fat diet (HFD; Research Diets, New Brunswick, NJ) for 12 weeks (24) ...
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No products found
because this supplier's products are not listed.
Seren Hamsici, Gokhan Gunay, Handan Acar,
bioRxiv - Bioengineering 2022
Quote:
... Fmoc protected amino acids (Gyros Protein Technologies) were removed through treatment with 20% piperidine/DMF solution for 45 min (three times for 15 min ...
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This membrane protein is Bovine FcRn (FCGRT & B2M) (23-298(FCGRT)&21-119(B2M)). It has been...
Cat# MP1450F,
1.0 case, Inquiry
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Xiaotian Tan, et al.,
bioRxiv - Bioengineering 2020
Quote:
... The human-cell-expressed SARS-CoV Spike S1-His recombinant protein was purchased from Creative Biolabs (VAng-Wyb7337). The recombinant CR3022 therapeutic antibody was purchased from Creative Biolabs (MRO-1214LC) ...
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No products found
because this supplier's products are not listed.
Tyler B. Waltz, et al.,
bioRxiv - Neuroscience 2023
Quote:
Recombinant rat protein p11 (S100A10 Recombinant Protein, Aviva Systems Biology, OPCD06771) was dissolved in distilled water to obtain a final concentration of 100ug/mL and stored at -80C for less than one month ...
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No products found
because this supplier's products are not listed.
Matthew S. Yorek, et al.,
bioRxiv - Microbiology 2019
Quote:
... We obtained our recombinant proteins from Tonbo biosciences which included mouse CXCL1 ...
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No products found
because this supplier's products are not listed.
Benjamin F. N. Campbell, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Binding of the FLAG-tagged DARPins to streptavidin-immobilized biotinylated Gephyrin variants was measured using FRET (donor: streptavidin-Tb, acceptor: anti-FLAG-d2, Cisbio). Further HTRF measurement against ‘No Target’ allowed for discrimination of Gephyrin-specific hits.
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No products found
because this supplier's products are not listed.
Chun Su, et al.,
bioRxiv - Genomics 2019
Quote:
... B2M and 18s rRNA transcripts was performed using Amplitaq Gold SYBR Master mix (ABI) on Applied Biosytems step one plus real-time thermocycler ...
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No products found
because this supplier's products are not listed.
I Ladero-Auñon, et al.,
bioRxiv - Immunology 2021
Quote:
... 1% penicillin-streptomycin and 40 ng/mL recombinant bovine M-CSF (Kingfisher Biotech, Inc. RP1353B) at 37°C and 5% CO2 ...
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No products found
because this supplier's products are not listed.
Reuben A. Saunders, et al.,
bioRxiv - Biophysics 2020
Quote:
... our ssrA-tagged titin protein was reacted at room temperature with three equivalents of ATTO 575Q maleimide (ATTO-TEC; GMBH) for 30 min in buffer A supplemented with 5 mM Tris (pH 8.0) ...
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No products found
because this supplier's products are not listed.
Sophie J. Hesketh, et al.,
bioRxiv - Cell Biology 2022
Quote:
For stable expression of mScarlet (mSc) tagged IFT-A proteins (IFT144, IFT140, IFT122, IFT121, and IFT139) and associated mutants using the Super PiggyBac system (System Biosciences), the relevant open reading frames were inserted into the PB-EF1α-MCS-IRES-Neo vector with C-terminal TEV ...
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No products found
because this supplier's products are not listed.
Teha Kim, et al.,
bioRxiv - Immunology 2023
Quote:
... The plates were blocked with PBS containing 1% BSA for 1 hour at room temperature before the specified biotinylated IgG1 M2e-MAbs or biotinylated IgG1 control MAbs (Clone: MOPC-21, BioXCell) diluted in PBS 0.1% Tween 20 (PBS-T ...
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No products found
because this supplier's products are not listed.
Dorothea Höpfner, et al.,
bioRxiv - Biochemistry 2020
Quote:
... samples containing 100 ng recombinant protein were run over a 5 μm Jupiter C4 300Å LC column (Phenomenex, Torrance, California) using the 1260 Infinity LC system (Agilent Technologies ...
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No products found
because this supplier's products are not listed.
Junjie Xu, et al.,
bioRxiv - Neuroscience 2023
Quote:
... The probe was tagged to opal 570 dye from Akoya Biosciences (Marlborough ...
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No products found
because this supplier's products are not listed.
Tingting Li, et al.,
bioRxiv - Biochemistry 2020
Quote:
... Biotinylated RBDavi was bound to streptavidin (Cat 16955, AAT Bioquest) which was fluorescently labeled by fluorescein via amine coupling ...
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No products found
because this supplier's products are not listed.
Anil H. Kadam, et al.,
bioRxiv - Bioengineering 2022
Quote:
... recombinant hu TGF-β1(ProSci, Poway, CA), anti-TGF-β1 IgY-Biotin (R&D Systems ...
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No products found
because this supplier's products are not listed.
Tatyana Veremeyko, et al.,
bioRxiv - Immunology 2024
Quote:
... Adult bovine plasma with sodium citrate and adult bovine serum were purchased from Innovative Research. Normal (NHS ...
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No products found
because this supplier's products are not listed.
Kui K. Chan, et al.,
bioRxiv - Biochemistry 2020
Quote:
... and anti-HIS-FITC (chicken polyclonal, 1/100 dilution; Immunology Consultants Laboratory) secondary antibodies for 20 minutes at 4°C ...
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No products found
because this supplier's products are not listed.
Liangxi Wang, et al.,
bioRxiv - Genomics 2022
Quote:
... 10 ng/ml recombinant human TNFα (Cell Applications, cat# RP1111-50) was added for 3 hours ...
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No products found
because this supplier's products are not listed.
Sangyoon J. Han, et al.,
bioRxiv - Cell Biology 2021
Quote:
... 10% Fetal Bovine Serum (Equitech-Bio, Inc, SFBU30), and 1% Anti-Anti (Gibco ...
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No products found
because this supplier's products are not listed.
Brian R. Shy, et al.,
bioRxiv - Synthetic Biology 2021
Quote:
... The solution is placed back on the magnet for 5 minutes and the supernatant containing the non-biotinylated strand is removed gently with non-stick pipettes and mixed immediately with Neutralization Buffer (100 uL 3M Sodium Acetate pH 5.2 and 4.9 mL 1X TE Buffer ...
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No products found
because this supplier's products are not listed.
Alina Remeeva, et al.,
bioRxiv - Biophysics 2021
Quote:
... with the mutation C85A and C-terminal 6×His tag) was obtained as a synthetic gene from Evrogen (Russia) and introduced into the pET11 expression vector (Novagen ...
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No products found
because this supplier's products are not listed.
Jamin Jung, et al.,
bioRxiv - Cell Biology 2021
Quote:
... a spinning disk confocal microscope (UltraVIEW VOX, Perkin-Elmer) equipped with an oil immersion objective Plan-Apochromat 100x/1.57 Oil-HI DIC (Carl Zeiss) was used ...
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No products found
because this supplier's products are not listed.
Isabell Ramming, et al.,
bioRxiv - Microbiology 2023
Quote:
... the bound Stx of STEC culture supernatants was detected using a biotinylated detection antibody (MBS311734 for Stx1, MyBioSource Inc., San Diego, USA; 11E10, hybridoma cell line from American Type Culture Collection, Manassas ...
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No products found
because this supplier's products are not listed.
Lindsay G. Serene, et al.,
bioRxiv - Microbiology 2023
Quote:
L929 fibroblasts were rapidly thawed in a 37°C water bath before being washed once in DMEM and 10% HI-FBS and transferred to a T-25 tissue culture treated flask (Sarstedt, Nümbrecht, Germany) in 10 mL DMEM and 10% HI-FBS with 100 U/mL penicillin/streptomycin ...
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No products found
because this supplier's products are not listed.
Michael J. Pereira, et al.,
bioRxiv - Microbiology 2021
Quote:
... or C1s proteins (Complement Technologies), or BSA (Sigma-Aldrich ...
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No products found
because this supplier's products are not listed.
David A. Lomas, et al.,
bioRxiv - Pharmacology and Toxicology 2020
Quote:
Crystallisation of recombinant Cys232Ser α1-antitrypsin was carried out in 2-well MRC crystallisation plates with a Mosquito robot (TTP Labtech) using 100nl protein solution and 100nl well solution ...
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No products found
because this supplier's products are not listed.
Aloysius Wong, et al.,
bioRxiv - Plant Biology 2019
Quote:
The UV-visible spectra of affinity purified recombinant DGK4 (200 µg/mL) was recorded on a PHERAstar FS micro-plate reader (BMG Labtech, USA). The heme environment of DGK4 was characterized by the addition of a reducing agent ...
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No products found
because this supplier's products are not listed.
Yeongjoon Lee, et al.,
bioRxiv - Biochemistry 2021
Quote:
... 15N-labeled SARS-CoV-2 nsp7 sample was expressed and purified by the same methods as previously described in our NMR assignment paper.20 The recombinant Escherichia coli Rossetta2 pLysS was first grown in a M9 minimal medium containing 1 g/L 15N4HCl (Cambridge Isotope Laboratories), 4 g/L 13C6-D-Glucose (Cambridge Isotope Laboratories ...
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No products found
because this supplier's products are not listed.
Joël S. Bloch, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... Protein Complex Suite (NeXtal) and PEG/Ion Suite (Hampton Research). Precipitant solution from crystallization screen was dispensed at 1:1 macromolecular complex/precipitant solution ratio in a total drop volume of 400 nL at room temperature ...