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No products found
because this supplier's products are not listed.
Pia Hempelmann, et al.,
bioRxiv - Cell Biology 2023
Quote:
... His-tagged protein was captured using NiNTA agarose beads (Cube Biotech) and eluted in 10 x 1 mL fractions with elution buffer containing 250 mM imidazole ...
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No products found
because this supplier's products are not listed.
Meenakshi Basu Shrivastava, et al.,
bioRxiv - Cell Biology 2020
Quote:
... 500 ng human recombinant His-tagged ubiquitin-conjugating enzyme (E2) Ube2d3 (from BIOMOL International, #U0880), in the presence or the absence of 10 μg N-terminal-His-tagged ubiquitin (Sigma-Aldrich ...
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No products found
because this supplier's products are not listed.
Aleksei Kuznetsov, et al.,
bioRxiv - Biochemistry 2020
Quote:
Human recombinant ACE2-His protein (Icosagen OÜ, Estonia, cat# P-302-100) and SARS-CoV-2 Spike protein S1 (Icosagen OÜ ...
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No products found
because this supplier's products are not listed.
Kanak Raina, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... 20 nM of His-tagged BRD4 BD1 and 20 nM of biotinylated histone H4 K5/8/12/16(Ac) peptide (AnaSpec) were mixed in 20 mM HEPES pH 7.5 ...
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No products found
because this supplier's products are not listed.
Hristina Vasileva, et al.,
bioRxiv - Immunology 2023
Quote:
... the SC and V2 domains were cloned into the pET15b expression vector as dual His (N-terminal) and Myc (C-terminal) tagged recombinant constructs (Biomatik, USA). A duplicate set of expression constructs were also cloned into the pGEX-4T-1 expression vector as single-tagged GST (N-terminal ...
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No products found
because this supplier's products are not listed.
Maxime Lecerf, et al.,
bioRxiv - Immunology 2020
Quote:
... Biotinylated recombinant human FcRn (Amsbio LLC, Cambridge, MA) was diluted to 5 μg/ml in running buffer - 100 mM Tris pH 6 (adjusted by addition of dry citric acid) ...
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No products found
because this supplier's products are not listed.
Chieh-Yu Liang, et al.,
bioRxiv - Immunology 2024
Quote:
Recombinant biotinylated XBB.1.5 spike protein was incubated with SPHERO Streptavidin fluorescent Yellow Particles (Spherotech, Cat. #SVF3- 2552) at 20 ng per μg beads for 30 min at room temperature on an end-over-end mixer ...
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No products found
because this supplier's products are not listed.
Suzanne G. Mays, et al.,
bioRxiv - Biochemistry 2021
Quote:
... expressing N-terminal Avi-tagged human LRH-1 and AAV8-GFP (used as a negative control) were obtained from Vector Biolabs (Malvern, PA, USA). C57BL/6J mice were administered 1×1012 genome copies (GC)/mL via femoral vein injection ...
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No products found
because this supplier's products are not listed.
Swann Floc’hlay, et al.,
bioRxiv - Developmental Biology 2020
Quote:
... Treatments with recombinant BMP2/4 (Recombinant Mouse BMP-4 Protein, CF 5020-BP/CF) or Nodal (Recombinant human Nodal protein 3218-ND-025/CF ...
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No products found
because this supplier's products are not listed.
Robert W. P. Glowacki, et al.,
bioRxiv - Microbiology 2019
Quote:
... Protein purification was accomplished using the pETite N-His vector (Lucigen). PCR primers were designed to amplify products for BT2807 and BT2808 containing all amino acids downstream of the predicted signal peptide sequences ...
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No products found
because this supplier's products are not listed.
Laurens H. Lindenburg, et al.,
bioRxiv - Biochemistry 2020
Quote:
... then loaded directly onto a Ni-NTA protein miniprep column (His Spin Protein Miniprep, Zymo Research). Protein was washed following the manufacturer’s instructions ...
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No products found
because this supplier's products are not listed.
Alanna B. Chan, et al.,
bioRxiv - Cell Biology 2021
Quote:
His-tagged mouse CRY2 D325H PHR domain (residues 1-512) was expressed in Sf9 suspension insect cells (Expression Systems, Davis, CA) infected with a P3 baculovirus at 1.2 × 106 cells per milliliter and grown for 72 hours at 27°C with gentle shaking ...
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No products found
because this supplier's products are not listed.
Senthil-Kumar Devan, et al.,
bioRxiv - Microbiology 2022
Quote:
... and for recombinant protein expression E .coli Lobstr cells (Kerafast EC1002) were used respectively ...
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No products found
because this supplier's products are not listed.
Krisztina Ötvös, et al.,
bioRxiv - Plant Biology 2019
Quote:
... The purified recombinant protein was used to immunize rabbits by a company (Eurogentec). From the immunserum a crude IgG fraction was isolated by ammonium sulfate precipitation then IgG was further purified on protein gel blots of the antigen ...
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No products found
because this supplier's products are not listed.
Raveen Rathnasinghe, et al.,
bioRxiv - Microbiology 2021
Quote:
... were maintained in Dulbecco’s Modified Eagle Medium (DMEM) complemented with 10% heat-inactivated Fetal Bovine Serum (HI-FBS; PEAK serum), penicillin-streptomycin (Gibco ...
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No products found
because this supplier's products are not listed.
Baira Godneeva, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... GFP- and FLAG-tagged proteins under the control of the Actin promoter using TransIT-LT1 reagent (Mirus). 24-40 h after transfection ...
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No products found
because this supplier's products are not listed.
Ben Johnson, et al.,
bioRxiv - Cell Biology 2023
Quote:
... Cells that were imaged were labeled using CF640-conjugated streptavidin to label biotinylated proteins (CF640R Streptavidin, Biotium cat 29041). Affinity purification was performed using streptavidin-conjugated magnetic beads (Pierce ...
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No products found
because this supplier's products are not listed.
Lucia Gonzalo, et al.,
bioRxiv - Plant Biology 2021
Quote:
... 4°C and the supernatants were incubated overnight with 1/100 RNAPII or HIS antibodies (RNAP II AS11 1804, HIS AS20 4441, Agrisera) and 30 μl SureBeads (BioRad) ...
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No products found
because this supplier's products are not listed.
Wei-Siang Liau, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Protein identification was carried out using Protein Pilot 5.0 (SCIEX) for database searching.
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No products found
because this supplier's products are not listed.
F Abou Azar, et al.,
bioRxiv - Biochemistry 2022
Quote:
... were isolated from wildtype mice or transgenic mice over-expressing a TAP-tagged 14-3-3ζ that were fed a low-fat (LFD) or 60% high-fat diet (HFD; Research Diets, New Brunswick, NJ) for 12 weeks (24) ...
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No products found
because this supplier's products are not listed.
Michael P. Doyle, et al.,
bioRxiv - Immunology 2022
Quote:
... his-tagged YFV E protein (Meridian Life Science) was associated to the tips at 5 μg/mL for 60 sec ...
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No products found
because this supplier's products are not listed.
Shuangshuo Jia, et al.,
bioRxiv - Cell Biology 2023
Quote:
... recombinant His-S ANXA1 protein (G15574, LifeSpan BioSciences, Seattle, WA, USA) was injected into the intra-articular cavity of SD rats four times weekly (1.3μg/50μL ...
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No products found
because this supplier's products are not listed.
Francesca Curreli, et al.,
bioRxiv - Pharmacology and Toxicology 2020
Quote:
... Various concentrations of biotinylated recombinant SARS-CoV-2 Spike RBD protein (Bioss Antibodies, Woburn, MA) dissolved in water were manually printed onto the Chip with Biodo bioprinting at 40% humidity via biotin-avidin conjugation ...
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No products found
because this supplier's products are not listed.
Rayan Khaddaj-Mallat, et al.,
bioRxiv - Neuroscience 2021
Quote:
... The levels of S protein in different brain regions upon its intranasal infusion were measured using His-Tag protein ELISA kit that allows detection and quantification of poly-histidine-tagged proteins in a biological sample (Cell Biolabs Inc., CA, USA). The experimental procedure was performed as recommended by the manufacturer’s instructions ...
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No products found
because this supplier's products are not listed.
Joanna Young, et al.,
bioRxiv - Microbiology 2020
Quote:
... Recombinant His-SFP1 was used to immunize female New Zealand white rabbits (Covalab) for generation of polyclonal antibodies ...
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No products found
because this supplier's products are not listed.
Kathryn L. Wofford, et al.,
bioRxiv - Bioengineering 2019
Quote:
... either human recombinant myelin basic protein (Alfa Aesar, cat. #AAJ66051LB0) fluorescently labeled with Vybrant DiD (Molecular Probes ...
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No products found
because this supplier's products are not listed.
Arun Upadhyay, et al.,
bioRxiv - Neuroscience 2023
Quote:
... 10 nM recombinant MT3 protein (Boster Bio Cat no. PROTP25713) was added at a in additional wells ...
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No products found
because this supplier's products are not listed.
Yoji Tsugawa, et al.,
bioRxiv - Microbiology 2024
Quote:
... The recombinant proteins were purified from supernatants by NGL COVID-19 Spike Protein Affinity Resin (Repligen) according to the manufacturer’s protocol ...
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No products found
because this supplier's products are not listed.
Jonathan M Downie, et al.,
bioRxiv - Genetics 2019
Quote:
... HEK293T cells were incubated in high glucose Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% heat inactivated fetal bovine serum (HI FBS, Genesee Scientific), and 1% pen/strep (P/S ...
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No products found
because this supplier's products are not listed.
Hsuan Cheng, et al.,
bioRxiv - Cell Biology 2021
Quote:
... The intensity of YFP-FKBP-tagged proteins at centrosomes upon rapamycin treatment was measured by Nikon Elements AR software.
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No products found
because this supplier's products are not listed.
Inga V. Hochheiser, et al.,
bioRxiv - Immunology 2021
Quote:
The purified and cross-linked recombinant NLRP3 protein was loaded onto glow-discharged EM-grids (Quantifoil, R2/1 Cu-covered with homemade ultrathin carbon support film) ...
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No products found
because this supplier's products are not listed.
Ella F.J. Halcrow, et al.,
bioRxiv - Cell Biology 2022
Quote:
... HeLa cells expressing the different GFP-tagged proteins were plated on an open μ-Slide with 8 wells (Ibidi, 80826) in complete medium containing 0.5 μM SiR-DNA dye (SpiroChrome) ...
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No products found
because this supplier's products are not listed.
Logan K. Smith, Kareem Fawaz, Bebhinn Treanor,
bioRxiv - Immunology 2020
Quote:
... were mixed with 2.5 ×10−4% biotinylated liposomes (N-Biotinylated Cap PE: Avanti Polar Lipids, Inc.). Alexa Fluor 633-streptavidin (1:1000 v/v ...
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No products found
because this supplier's products are not listed.
Clàudia Salat-Canela, et al.,
bioRxiv - Cell Biology 2021
Quote:
... Recombinant proteins were checked and quantified in a polyacrylamide gel stained with Comassie Brilliant Blue (Amresco, 0472). The proteins were stored at −80°C ...
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No products found
because this supplier's products are not listed.
Pablo Lazcano, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... Corresponding europium-tagged secondary antibodies (Molecular Devices) were used at a 1:5000 dilution and 1:20,000 for actin ...
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With the RapidClean protein removal kit, completely remove protein from aqueous solutions of...
Cat# K-01001-010,
10 reactions, USD $145.00/ea
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Lukas Kunz, et al.,
bioRxiv - Plant Biology 2022
Quote:
... for FLAG tagged effectors or WesternBright ECL HRP substrate (Advansta) for Pm8-HA.
-
No products found
because this supplier's products are not listed.
Chungwon Park, et al.,
bioRxiv - Neuroscience 2020
Quote:
... or the biotinylated anti-VGAT antibody (131 103CpH, Synaptic Systems) was conjugated to streptavidin-conjugated quantum dots (cat ...
-
No products found
because this supplier's products are not listed.
Andrew J. Stout, et al.,
bioRxiv - Bioengineering 2022
Quote:
... or Beefy-R (Hi-Def B8 supplemented with 0.4 mg/mL RPI). Beefy-9 and Beefy-R were prepared immediately before use ...
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No products found
because this supplier's products are not listed.
Anissa A. Widjaja, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... 2% fetal bovine serum (0010, ScienCell), 1% fibroblast growth supplement (2352 ...
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No products found
because this supplier's products are not listed.
Daniel Aguilar-Hidalgo, et al.,
bioRxiv - Developmental Biology 2022
Quote:
... 0.025% Bovine serum albumin (Wisent Bioproducts),12 ng/ml FGF2 (Peprotech ...
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No products found
because this supplier's products are not listed.
Nicole LaMassa, et al.,
bioRxiv - Neuroscience 2020
Quote:
... 20 mM NaCl and incubated with secondary 15 nm gold-tagged goat-anti-rabbit IgG F(ab’)2 (Electron Microscopy Sciences) diluted 1:10 in 20 mM Tris pH 7.4 ...
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No products found
Daniel M. Sapozhnikov, Moshe Szyf,
bioRxiv - Molecular Biology 2020
Quote:
... and dCas9 recombinant protein (Applied Biological Materials Inc.). After 10 minutes at room temperature ...
-
No products found
because this supplier's products are not listed.
MEENAKSHI TETORYA, et al.,
bioRxiv - Plant Biology 2023
Quote:
... The His-tagged peptides (GMA4CG_WT and GMA4CG_V6) phospholipid binding assays were performed using the PIP StripsTM (Echelon Biosciences Inc., CA) as previously described (Islam et al. ...
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Recombinant protein produced in Pichia pastoris. Free of animal derived components, RNases, and...
Cat# LS006361,
10 ku, $200.00
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Lingling Yin, et al.,
bioRxiv - Plant Biology 2023
Quote:
... Proteins were digested by adding bovine trypsin (Worthington-LS003750) and endoproteinase Lys-C (NEB-P8109S ...
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No products found
because this supplier's products are not listed.
Jennifer H. Kong, et al.,
bioRxiv - Developmental Biology 2020
Quote:
... Biotinylated proteins from clarified supernatants were captured on a streptavidin agarose resin (TriLink Biotechnologies), washed once with Biotinylation Lysis Buffer A ...
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No products found
because this supplier's products are not listed.
Francois Chesnais, et al.,
bioRxiv - Bioengineering 2022
Quote:
... and Red Fluorescent Protein-tagged HUVEC (RFP-HUVEC, 2Bscientific, UK) were cultured on fibronectin-coated (PromoCell) plates up to passage 7 and maintained in Endothelial Growth Medium 2 (EGM2 ...
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No products found
because this supplier's products are not listed.
Anne Mohrholz, et al.,
bioRxiv - Plant Biology 2019
Quote:
... the equal content of GFP-tagged fusion protein in the crude extracts was adjusted according to the GFP fluorescence using a fluorescence reader (TECAN Safire).
-
No products found
because this supplier's products are not listed.
Camden R. Bair, et al.,
bioRxiv - Microbiology 2021
Quote:
... supplemented with 8% heat-inactivated newborn calf serum (HI-NBCS, Rocky Mountain Biologicals), 1.5 g/ml Na2CO3 (Amresco) ...
-
No products found
because this supplier's products are not listed.
Diogo M. Lourenço, et al.,
bioRxiv - Neuroscience 2023
Quote:
... and 3% bovine serum albumin (BSA, #MB04602, NZYTech) for 1.5h was performed to block nonspecific binding sites ...
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No products found
because this supplier's products are not listed.
Jack A. Bryant, et al.,
bioRxiv - Microbiology 2020
Quote:
Purified recombinant BepA was used with proprietary crystal screens (supplied by Molecular Dimensions and Jena Bioscience) in sitting drop crystallization experiments using 2 μl of protein solution and 2 μl of crystallization mother liquor at 18°C ...