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magnetofection
difficult to transfect cells
Cat# KC30296,
PolyMag 100µL+PolyMag Neo100µL+ CombiMag 100µL+Magnetic Plate MF96000, USD $640.63/KIT
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Andrew S. Flies, et al.,
bioRxiv - Immunology 2020
Quote:
... Digested proteins in PBS were diluted 1:1 in Squalvax (Oz Biosciences # SQ0010) to a final concentration of 0.1 μg/μL and was mixed using interlocked syringes to form an emulsion ...
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No products found
because this supplier's products are not listed.
Peter R. Corridon,
bioRxiv - Bioengineering 2021
Quote:
... scaffolds were suspended in approximately 400-500 ml of heparinized pig whole blood (BioIVT, Westbury, NY, USA). The Ismatec MCP-Z Process or MCP-Z Standard dispensing pumps (Cole-Palmer ...
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No products found
because this supplier's products are not listed.
Leticia R. Q. Souza, et al.,
bioRxiv - Neuroscience 2023
Quote:
... the primary antibodies [Non-structural protein 1 from ZIKV (NS1, 1:500, BioFront Technologies, BF1225-06;), Class III β-tubulin (TuJ3 ...
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No products found
because this supplier's products are not listed.
Joshua D. Powell, et al.,
bioRxiv - Microbiology 2024
Quote:
Swine antisera previously produced by immunizing each of two pigs was treated with receptor-destroying enzyme II (Hardy Diagnostics, Santa Maria, CA), heat inactivated at 56 °C ...
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No products found
because this supplier's products are not listed.
Y Vicioso, et al.,
bioRxiv - Immunology 2021
Quote:
... and proteins were crosslinked to DNA in two steps: (1) by adding DSG (Disuccinimidyl glutarate; ProteoChem) to a final concentration of 2mM for 45 minutes at room temperature followed by washing once with PBS and (2 ...
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No products found
because this supplier's products are not listed.
Guoling Li, et al.,
bioRxiv - Bioengineering 2019
Quote:
Genomic DNA from ear tissue of founders (F0) pigs was isolated according to the protocol of the OMEGA Kit (OMEGA Bio-Tek, Georgia, USA) for PCR and southern blot analysis ...
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No products found
because this supplier's products are not listed.
Alexandra Maslennikova, et al.,
bioRxiv - Microbiology 2021
Quote:
The levels of HIV-1 core protein Gag in cell supernatants were quantified using HIV-1 p24 ELISA Kit (Zeptometrix, Bufalo NY, USA) in accordance to the manufacturer’s instruction ...
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No products found
because this supplier's products are not listed.
Petra Kangas, et al.,
bioRxiv - Cell Biology 2022
Quote:
... For depletion of the most abundant proteins from CSF ProteoSpin Abundant Serum Protein depletion kit (Norgen Biotek) was used according to manufacturer’s instructions ...
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No products found
because this supplier's products are not listed.
Alexis Bouin, et al.,
bioRxiv - Microbiology 2022
Quote:
... diluted 1:500 in blocking solution for detection of viral protein VP1 or anti dsRNA antibody (SCICONS, anti-dsRNA mAb J2, 10010500) diluted 1:1000 in blocking solution overnight at 4°C ...
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No products found
because this supplier's products are not listed.
Li-Ying Yu, et al.,
bioRxiv - Neuroscience 2023
Quote:
... Recombinant MANF protein (P-101-100, Icosagen) in PBS at 200 ng/ul was used as positive control ...
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No products found
because this supplier's products are not listed.
Takanori Eguchi, et al.,
bioRxiv - Molecular Biology 2019
Quote:
... Cells were blocked in IHC/ICC blocking buffer high protein (eBioscience, San Diego, CA) for 10 min and then reacted with anti-MZF1 antibody (1:50, C10502; Assay Biotechnology, Fremont, CA) and AlexaFluor488 secondary antibody (Thermo Fisher Scientific ...
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No products found
because this supplier's products are not listed.
Michael P. Doyle, et al.,
bioRxiv - Immunology 2022
Quote:
... his-tagged YFV E protein (Meridian Life Science) was associated to the tips at 5 μg/mL for 60 sec ...
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No products found
because this supplier's products are not listed.
Sandro Roier, et al.,
bioRxiv - Immunology 2023
Quote:
... The protein vaccines were alum-adjuvanted by adsorption of the recombinant fusion proteins to aluminum hydroxide (Alhydrogel®; Croda, Frederikssund, Denmark) as previously described22 ...
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No products found
because this supplier's products are not listed.
Qianyan Li, et al.,
bioRxiv - Genetics 2022
Quote:
... and the column was washed with lysis buffer to remove unbound proteins before eluting the proteins with 50 μM biotin (Chem-Impex Int’l) in low salt buffer (20mM HEPES-KOH pH 7.2 ...
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No products found
because this supplier's products are not listed.
Moon Kee Meang, et al.,
bioRxiv - Cell Biology 2022
Quote:
... Protein bands were visualized using ECL reagent (Abfrontier, Korea) and Uvitec HD9 (Uvitec ...
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No products found
because this supplier's products are not listed.
Deborah L. Gater, et al.,
bioRxiv - Biophysics 2022
Quote:
... Vitamin D binding protein (DBP) was purchased from Athens Research and Vitamin D Binding protein (VDR ...
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No products found
because this supplier's products are not listed.
Osamu Udagawa, et al.,
bioRxiv - Developmental Biology 2021
Quote:
... anti-70-kDa heat shock proteins (Hsp70) (StressMarq Biosciences, Victoria, Canada), anti-splicing component ...
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No products found
because this supplier's products are not listed.
Xavier Martiáñez-Vendrell, et al.,
bioRxiv - Microbiology 2019
Quote:
... vivax pLDH proteins expressed in insect cells (3001, ReliaTech GmbH, Germany) were used as reference material ...
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Recombinant Antigen
Cat# DENV1-NS1-100,
100µg USD $274.0
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Alexander A. Lehmann, et al.,
bioRxiv - Immunology 2020
Quote:
... truncated Spike protein (S1 domain) (The Native Antigen Company, Oxford, UK) or receptor binding domain (RBD ...
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No products found
because this supplier's products are not listed.
Megha Subramanian, et al.,
bioRxiv - Genomics 2022
Quote:
... TTR protein levels were measured with mouse prealbumin kit from ALPCO, 41-PALMS-E01 (serum diluted 1:4000) ...
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No products found
because this supplier's products are not listed.
Beatriz Gil, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Recombinant alpha- synuclein protein was a gift from rPeptide (GA, USA).
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No products found
because this supplier's products are not listed.
Alicia Kairouani, et al.,
bioRxiv - Plant Biology 2023
Quote:
... proteins were extracted using a Constant cell disrupter system (Constant Systems) with a disruption pressure of 2.35kbar and then purified using His-bind buffer kit (Millipore ...
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No products found
because this supplier's products are not listed.
Yirui Li, Julie A. Champion,
bioRxiv - Bioengineering 2024
Quote:
OVA protein vesicles were imaged by a TEM (JEM 100CX-II, JEOL). 5 μL of protein vesicle solution was dropped on a copper grid (Electron Microscopy Sciences ...
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No products found
because this supplier's products are not listed.
JM Robinson, et al.,
bioRxiv - Immunology 2019
Quote:
... and LBP (Human Lipopolysaccharide Binding Protein ELISA Kit, Cell Sciences, Inc., Cat# CKH113).
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No products found
because this supplier's products are not listed.
Daniele Merico, et al.,
bioRxiv - Genetics 2019
Quote:
... PVDF membrane was cut at 75 kDa according to protein ladder (BlueElf, FroggaBio). The higher molecular weight portion of the membrane was incubated with ATP7B antibody (Abcam ...
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Cat# G209,
USD $10.00/EA
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Samantha Sarni, et al.,
bioRxiv - Biochemistry 2020
Quote:
... purified Gag proteins were dialyzed (3500 MWCO dialysis tubing, BioDesign Inc. of New York) into 500 mM ammonium acetate ...
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No products found
because this supplier's products are not listed.
Jeremy Ariey-Bonnet, et al.,
bioRxiv - Pharmacology and Toxicology 2020
Quote:
... The reagents (compound, protein, and fluorophore) were dispensed using an Echo550 acoustic dispenser (Labcyte): 100 nL of compound (from a 100% DMSO stock at 10 mM ...
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No products found
because this supplier's products are not listed.
David S. Yang, et al.,
bioRxiv - Biophysics 2020
Quote:
... Protein and incubation buffer were filtered with 0.22 μm Polyethersulfone (PES) (CELLTREAT Scientific Products, 229746) syringe filters ...
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No products found
because this supplier's products are not listed.
Jenny M. Mannion, et al.,
bioRxiv - Immunology 2023
Quote:
... emulsified 1:1 in 100μl CFA (Chondrex Inc.), containing 4mg/ml H37Ra Mtb ...
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No products found
because this supplier's products are not listed.
Venkatesh Mallikarjun, et al.,
bioRxiv - Cell Biology 2022
Quote:
... Proteins from cell pellets were solubilised by bead-beating using six 1.6 mm steel beads (Next Advance) at 4 °C in 30 µL 1.1% (w/v ...
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No products found
because this supplier's products are not listed.
Katy R. McCarron, et al.,
bioRxiv - Cell Biology 2023
Quote:
... anti-LC3 (1:200 WB, 1:200 IF, Nanotools; 5F10), anti-p62 (1:1,000 WB ...
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No products found
because this supplier's products are not listed.
Hammam Antar, et al.,
bioRxiv - Biochemistry 2021
Quote:
... CTP/parSDNA 40 pre-mix (10 μL) was added to protein solution (10 μL) using BenchSmart 96 (Rainin) dispenser robot and mixed by pipetting ...
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No products found
because this supplier's products are not listed.
Pooja Kesari, et al.,
bioRxiv - Biophysics 2021
Quote:
... PEG 8000) with 0.4 µl protein + 0.4 µl reservoir as sitting drops in 96-well XJR trays (Rigaku Reagents) at 14°C ...
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No products found
because this supplier's products are not listed.
Luis F. Garcia-Alles, et al.,
bioRxiv - Biochemistry 2019
Quote:
... protein tags were removed by incubation overnight at 25°C with turbo TEV protease (GenWay, 5 μg/mL final) in 50 mM Tris pH 8.0 / 300 mM NaCl /2 mM DTT/ 1 mM EDTA ...
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No products found
because this supplier's products are not listed.
Kritika S. Katiyar, et al.,
bioRxiv - Bioengineering 2019
Quote:
... 1% FBS (Atlanta Biologicals), 2.5 mg/mL glucose (Sigma) ...
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No products found
because this supplier's products are not listed.
Sarah L. Olguin, et al.,
bioRxiv - Neuroscience 2020
Quote:
... NbActive-1 medium (BrainBits) supplemented with 100 U/ml of Penicillin-Streptomycin (Life Technologies ...
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No products found
because this supplier's products are not listed.
Nicholas N. Foster, et al.,
bioRxiv - Neuroscience 2020
Quote:
... fluorogold (FG, 1%; Fluorochrome); and cholera toxin subunit B-AlexaFluor 647 conjugate (CTB ...
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No products found
because this supplier's products are not listed.
Ewan Phillip Ramsay, et al.,
bioRxiv - Biochemistry 2020
Quote:
HeLa cells were transfected with a 1:1:1 mix of gRNA1 and gRNA2 vectors together with the donor plasmid using PolyJet transfection reagent (SL100688, SignaGen Laboratories) according to the manufacturer’s instructions ...
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No products found
because this supplier's products are not listed.
Haylie R. Helms, et al.,
bioRxiv - Bioengineering 2024
Quote:
GFP+ HUVEC and RFP+ MDA-MB-231 were printed onto a collagen type 1 substrate and in cultured in a 1:1 ratio of HUVEC media (VascuLife VEGF, Lifeline Cell Technology) and MDA-MB-231 media (DMEM + 10% FBS + 1% penicillin-streptomycin) ...
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No products found
because this supplier's products are not listed.
Michael Adams, et al.,
bioRxiv - Biochemistry 2021
Quote:
... with peptides generated from roughly 500ng of proteins injected for each analysis onto either 75 micron x 25cm packed emitter columns (New Objective) or 75 micron x 50 cm C18 Acclaim Pepmap columns (Thermo Scientific) ...
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No products found
because this supplier's products are not listed.
Wenwei Li, et al.,
bioRxiv - Microbiology 2021
Quote:
... and 1 mM dithiothreitol) and 50 μL of 1 mM D-luciferin potassium salt (Prolume). The neutralization half-maximal inhibitory dilution (IC50 ...
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No products found
because this supplier's products are not listed.
Jonathon A.B. Smith, et al.,
bioRxiv - Physiology 2024
Quote:
... and glucose uptake was measured in the same medium by adding 1 uL.mL-1 of 2-[1,2-3H]Deoxy-D-glucose (MT911, Moravek) and 10 μM unlabelled 2-Deoxy-D-glucose in for 15 min [32] ...
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No products found
because this supplier's products are not listed.
Akul Singhania, et al.,
bioRxiv - Immunology 2021
Quote:
... 1% penicillin/streptomycin (Omega Scientific, Tarzana, CA), and 50 U/ml Benzonase (Millipore Sigma ...
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No products found
because this supplier's products are not listed.
Xin Lai, et al.,
bioRxiv - Systems Biology 2020
Quote:
... 1 000 U/mL IL-6 (CellGenix), 10 ng/mL TNF (Beromun ...
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No products found
because this supplier's products are not listed.
Julia Obergasteiger, et al.,
bioRxiv - Neuroscience 2021
Quote:
... and TH (Pel-Freez Biologicals, P60101, 1:1000) antibodies ...
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No products found
because this supplier's products are not listed.
Thorben Schramm, Vanessa Pahl, Hannes Link,
bioRxiv - Systems Biology 2023
Quote:
... covered with Breathe-Easy (Diversified Biotech BEM-1) adhesive membrane ...
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No products found
because this supplier's products are not listed.
Moitrayee Bhattacharyya, et al.,
bioRxiv - Biophysics 2019
Quote:
... the glass substrates were treated with a mixture of Poly-L-lysine PEG and PEG-Biotin (1000:1, both at 1 mg/mL) for 30 minutes (SuSoS, Dübendorf, Switzerland). The glass substrates were then washed with 2 mL of Dulbecco’s phosphate-buffered saline (DPBS ...
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No products found
because this supplier's products are not listed.
D.E. Selley, et al.,
bioRxiv - Pharmacology and Toxicology 2019
Quote:
... Bilateral tissue punches 1–1.5 mm in length were taken with a 1 mm internal diameter corer (Fine Science Tools; Foster City, CA) and placed in Eppendorf tubes kept on dry ice and then stored at −80° C ...
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No products found
because this supplier's products are not listed.
Tao Zhang, et al.,
bioRxiv - Plant Biology 2022
Quote:
... and 1/16” stainless steel tubing were acquired from IDEX Health & Sciences (Oak Harbor ...
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No products found
because this supplier's products are not listed.
Caroline Murawski, et al.,
bioRxiv - Neuroscience 2020
Quote:
... and 2 µm S1818 (Microchem, 5000 rpm, baking at 100°C for 1 min). Patterns were developed for 40 – 50 s in MF319 (Microchem) ...