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No products found
because this supplier's products are not listed.
Adem Yildirim, et al.,
bioRxiv - Cell Biology 2021
Quote:
... CF 488 (donkey-anti-guinea pig, 20169-1, Biotrend, Destin, USA), Alexa488 (donkey-anti-mouse ...
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No products found
because this supplier's products are not listed.
Thibault Legal, et al.,
bioRxiv - Cell Biology 2020
Quote:
... Cheeseman, 1: 1000), guinea pig CENP-C (pAb, MBL PD030, 1:2000) and human ACA antibodies (Cambridge Biosciences, 1:100). Hoechst 33342 (ThermoFisher Scientific ...
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No products found
Haitao Luan, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... CAV1 sgRNA CRISPR/Cas9 All-in-One Lentivector (pLenti-U6-sgRNA-SFFV-Cas9-2A-Puro) from Applied Biological Materials (Richmond, BC, Canada) was used to derive CAV1 KO cell lines ...
-
No products found
because this supplier's products are not listed.
Evelína Šťastná, et al.,
bioRxiv - Immunology 2023
Quote:
... A biotinylated rabbit anti-pig antibody (clone KPB0499S-050, Kingfisher Biotech) at 100 ng/mL was used for detection ...
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No products found
because this supplier's products are not listed.
Coralie Zangarelli, et al.,
bioRxiv - Genomics 2022
Quote:
A peptide corresponding to PgmL1 amino acid sequence 1 to 266 and carrying a C-terminal His tag was used for guinea pig immunization (Proteogenix). Sera were purified by antigen affinity purification to obtain highly specific α−PgmL1-GP antibodies (0.8 mg/mL) ...
-
No products found
because this supplier's products are not listed.
Chiara Galante, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Red Fluorescent Protein (RFP, rabbit, 1:500, Biomol, 600401379S); SRY-Box 10 (Sox10 ...
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No products found
because this supplier's products are not listed.
Nisha Dhanushkodi, et al.,
bioRxiv - Immunology 2022
Quote:
... using 100 μg CpG/guinea pig and 150μg alum (Alhydrogel, Accurate Chemical and Scientific Corp., Westbury, NY).
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No products found
because this supplier's products are not listed.
Gab-Chol Choi, et al.,
bioRxiv - Bioengineering 2020
Quote:
... and bone morphogenetic protein 2 (BMP-2) (1:200, orb251474, Biorbyt) primary antibodies at 4°C ...
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No products found
because this supplier's products are not listed.
Sk. Kayum Alam, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... were incubated with 1 μg commercially available human IKKα protein (SignalChem) for 30 minutes at 30°C ...
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No products found
because this supplier's products are not listed.
Kazi T Haq, et al.,
bioRxiv - Physiology 2023
Quote:
... isolated hearts were loaded with a potentiometric dye (RH237: 62 μg rat or guinea pig heart; AAT Bioquest). Fluorescent signals were acquired by illuminating the epicardial surface with a light emitting diode equipped with excitation filter (525 ± 25 nm ...
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No products found
because this supplier's products are not listed.
Li Dai, et al.,
bioRxiv - Biophysics 2021
Quote:
... 1% w/v 800 nm Protein G-coated polystyrene beads (Spherotech Inc) were washed in the same way as the streptavidin beads ...
-
No products found
because this supplier's products are not listed.
Therese de Neergaard, et al.,
bioRxiv - Immunology 2022
Quote:
Human monocytic cell line Tamm–Horsfall protein 1 (THP-1) (TIB-202, male; American Type Culture Collection) was cultured in RPMI 1640 medium (Sigma-Aldrich ...
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No products found
because this supplier's products are not listed.
Vitor Mendes, et al.,
bioRxiv - Biochemistry 2020
Quote:
... was mixed in 1:1 and 1:2 (protein to reservoir) ratio with well solution using a mosquito robot (TTP labtech). Initial conditions were obtained in the Classics lite crystallization screen (Qiagen) ...
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No products found
because this supplier's products are not listed.
Emily G. Armbruster, et al.,
bioRxiv - Cell Biology 2023
Quote:
... and protein expression was induced by IPTG (isopropyl ß-D-1-thiogalactopyranoside, Teknova) as indicated ...
-
No products found
because this supplier's products are not listed.
Katherine J. Turner, et al.,
bioRxiv - Neuroscience 2022
Quote:
Rabbit anti-green fluorescent protein (GFP; Torrey Pines Biolabs, Cat# TP401, dilution 1:1000), rat anti-GFP (Nacalai Tesque ...
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No products found
because this supplier's products are not listed.
Justin A. Jarrell, et al.,
bioRxiv - Bioengineering 2019
Quote:
... each sample was mixed in a 1.5mL tube with the appropriate volume of EGFP mRNA (996 nucleotides translated into a 26.9 kDa protein, 1 mg mL−1 L-7601, TriLink BioTechnologies) at final concentrations ranging from 10 μg mL−1 to 160 μg mL−1 (30 nM to 473 nM) ...
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No products found
because this supplier's products are not listed.
Nguyen-Vi Mohamed, et al.,
bioRxiv - Neuroscience 2021
Quote:
... and chicken anti-microtubule associated protein 2 (MAP2, 1:500, EnCor Biotechnology Inc, FL, USA), followed by an incubation (3 days ...
-
No products found
because this supplier's products are not listed.
Celeste M. Sheftel, Laura L. Hernandez,
bioRxiv - Molecular Biology 2020
Quote:
... Protein concentration was determined using bicinchoninic acid assay (Bioworld, catalog number 20831001-1, Dublin, OH). Protein lysates were diluted to 1.5μg/μl with 5x sample buffer containing SDS and β-mercaptoethanol and was heated at 95°C for 10 minutes ...
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No products found
because this supplier's products are not listed.
Shafali Gupta, et al.,
bioRxiv - Cell Biology 2021
Quote:
... Rabbit antibodies against the following proteins were used: GAPDH (Trevigen 2275-PC-100; 1:5000), p114 RhoGEF (Abcam ab96520 ...
-
No products found
because this supplier's products are not listed.
Jonathan T. Lloyd, et al.,
bioRxiv - Biochemistry 2020
Quote:
... 66 were reproduced in hanging drops (1 μL protein solution plus 1 μL mother liquor) using 24-well VDX plates (Hampton Research) containing 500 μL mother liquor in the reservoir ...
-
No products found
because this supplier's products are not listed.
Katherine M Bauer, et al.,
bioRxiv - Biochemistry 2019
Quote:
... For unlabeled protein expression was induced with 1 mM isopropylthio-β-D-galactoside (IPTG; Amresco, 97061) and cultures were grown for 18 hours at 18°C ...
-
No products found
because this supplier's products are not listed.
Tyler B. Waltz, et al.,
bioRxiv - Neuroscience 2023
Quote:
Recombinant rat protein p11 (S100A10 Recombinant Protein, Aviva Systems Biology, OPCD06771) was dissolved in distilled water to obtain a final concentration of 100ug/mL and stored at -80C for less than one month ...
-
No products found
because this supplier's products are not listed.
Marko Manevski, et al.,
bioRxiv - Pharmacology and Toxicology 2022
Quote:
The protein levels of ICAM-1 and IL-6 were determined using human ELISA kits against ICAM-1 (LifeSpan Biosciences Inc., Seattle, WA) and IL-6 (BioLegend Inc. ...
-
No products found
because this supplier's products are not listed.
El Batoul Djouani-Tahri, et al.,
bioRxiv - Plant Biology 2023
Quote:
... Total cellular proteins were labeled by adding 5 μCi mL-1 of [35S] Na2SO4 (American Radiolabeled Chemicals) for 10 min ...
-
No products found
because this supplier's products are not listed.
Brunno R. Levone, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... Proteins were quantified using a validated BCA (Bicinchoninic Protein Assay Kit, EuroClone) protocol ...
-
No products found
because this supplier's products are not listed.
April H. Nguyen, et al.,
bioRxiv - Microbiology 2023
Quote:
Protein-protein interactions were evaluated using the Bacterial Two Hybrid System (Euromedex) according to the manufacturer instructions ...
-
No products found
because this supplier's products are not listed.
Matthäus Mittasch, et al.,
bioRxiv - Cell Biology 2019
Quote:
... Protein-Free (Expression Systems), supplemented with Fetal Bovine Serum (2% final concentration).
-
No products found
because this supplier's products are not listed.
Tshegofatso Ngwaga, et al.,
bioRxiv - Microbiology 2023
Quote:
Protein carbonyls were visualized using the OxiSelect Protein Carbonyl Immunoblot Kit (Cell Biolabs). Tnrf1-/- BMDMs were seeded in 24-well plates at 2.5x105 one day prior to infection with indicated L ...
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No products found
because this supplier's products are not listed.
Marco Di Gioia, et al.,
bioRxiv - Immunology 2023
Quote:
... Total protein concentrations were determined using BCA protein assay kit (Genesee Scientific, Cat# 18-440). Proteins were separated using SDS-PAGE electrophoresis and then transferred to polyvinylidene difluoride (PVDF ...
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96 well glass bottom plate. Black polystyrene frame with #1 glass(0.13-0.16mm), with lid,...
Cat# P96-1-N,
20/case, $226.00
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Minghui Di, et al.,
bioRxiv - Molecular Biology 2023
Quote:
HeLa cells were transfected with DNA (1 ug) expressing mCherry-tagged proteins cultured on 29-mm glass-bottom dish (Cellvis); plasmids (1 ug ...
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No products found
because this supplier's products are not listed.
Yifang Liu, et al.,
bioRxiv - Molecular Biology 2022
Quote:
Recombinant spike protein (BPS bioscience #100810) was captured by ACE2 receptor protein (Acro biosystems #AC2-H82F9 ...
-
No products found
because this supplier's products are not listed.
Drake M. Mellott, et al.,
bioRxiv - Biochemistry 2020
Quote:
... Enrichment of alkyne-modified proteins was conducted using the Click-&-Go Protein Enrichment Kit (Click Chemistry Tools) and the protocols thereof ...
-
No products found
because this supplier's products are not listed.
Basel M. Al-Barghouthi, et al.,
bioRxiv - Genetics 2021
Quote:
... high protein diet (5% fat and 20.3% protein) (D12109C and D12083101, respectively, Research Diets, New Brunswick, NJ), and were euthanized and analyzed at 24–25 weeks of age ...
-
No products found
because this supplier's products are not listed.
Isabell Bludau, et al.,
bioRxiv - Systems Biology 2022
Quote:
The molecular weight calibration curve for SEC fractionation was obtained by running a protein standard mix (Column Performance Check Standard, Aqueous SEC 1, AL0-3042, Phenomenex, CA, USA) before each sample injection (Supplementary Table 19).
-
No products found
because this supplier's products are not listed.
Seren Hamsici, Gokhan Gunay, Handan Acar,
bioRxiv - Bioengineering 2022
Quote:
... Fmoc protected amino acids (Gyros Protein Technologies) were removed through treatment with 20% piperidine/DMF solution for 45 min (three times for 15 min ...
-
No products found
because this supplier's products are not listed.
Yann Aquino, et al.,
bioRxiv - Genomics 2022
Quote:
... Recombinant IFN-χ protein (PBL Assay Science) was used as a calibrator ...
-
No products found
because this supplier's products are not listed.
Yuma Nishikawa, et al.,
bioRxiv - Synthetic Biology 2024
Quote:
... Protein was extracted from the cells using E.coli/Yeast Protein Extraction Buffer (Tokyo Chemical Industry Co., Ltd., Tokyo, Japan), and the lysate fraction was used for analysis.
-
No products found
because this supplier's products are not listed.
Matthew S. Yorek, et al.,
bioRxiv - Microbiology 2019
Quote:
... We obtained our recombinant proteins from Tonbo biosciences which included mouse CXCL1 ...
-
No products found
because this supplier's products are not listed.
Vikas A. Tillu, et al.,
bioRxiv - Cell Biology 2020
Quote:
... Cell were propogated in either LB or TB media and protein expression was performed by inducing with 0.5 mM IPTG (Isopropyl ß-D-1-thiogalactopyranoside, Bioline, Cat No. BIO-37036) overnight at 180C ...
-
No products found
because this supplier's products are not listed.
Robin-Lee Troskie, et al.,
bioRxiv - Genomics 2021
Quote:
... Protein concentration was measured using the Pierce™ BCA Protein Assay Kit on the POLARstar® Omega microplate reader (BMG Labtech). 7μg of protein lysate was diluted with 4x Laemmli Sample Buffer (BioRad cat # 1610747 ...
-
No products found
because this supplier's products are not listed.
Kacey Mersch, et al.,
bioRxiv - Biophysics 2020
Quote:
... and protein eluted with CoWB containing 400 mM Imidazole (RPI). The eluate was concentrated with a 10,000 MWCO centrifugal filter (Millipore-Sigma ...
-
No products found
because this supplier's products are not listed.
Kimberley R.G. Cortenbach, et al.,
bioRxiv - Immunology 2021
Quote:
... protein blocking in Akoya Antibody Diluent/Block (Akoya biosciences, MA) took place for 10 minutes ...
-
No products found
because this supplier's products are not listed.
Grant A. King, et al.,
bioRxiv - Cell Biology 2019
Quote:
... Protein extracts were generated by pulverization using a Mini-Beadbeater-96 (BioSpec). The samples were then treated with 50 µl of 3X SDS sample buffer (187.5 mM Tris pH 6.8 ...
-
No products found
because this supplier's products are not listed.
Daniela Saderi, Brad N. Buran, Stephen V. David,
bioRxiv - Neuroscience 2019
Quote:
... Their diet was supplemented with 20 ml/day of high protein Ensure (Abbott). Water restriction was to be discontinued if weight dropped below 20% of the initial weight ...
-
No products found
because this supplier's products are not listed.
Lennart Schada von Borzyskowski, et al.,
bioRxiv - Synthetic Biology 2022
Quote:
... all protein digests were desalted using C18 microspin columns (Harvard Apparatus, Holliston, USA) according to the manufacturer’s instructions.
-
No products found
because this supplier's products are not listed.
Charles R. Heller, et al.,
bioRxiv - Neuroscience 2020
Quote:
... 1 - 4 tungsten micro-electrodes (FHC, 1-5 MΩ) were inserted to characterize the tuning and response latency of the region of cortex ...
-
No products found
because this supplier's products are not listed.
Srinivasan Sivanandan, et al.,
bioRxiv - Genomics 2023
Quote:
... 1 mL of recovery media (Biosearch Technologies, 80026-1) was transferred to each culture tube in advance ...
-
No products found
because this supplier's products are not listed.
Rana Soylu-Kucharz, et al.,
bioRxiv - Neuroscience 2023
Quote:
... vasopressin (1:10000, Chemicon) and oxytocin (1:2000, Phoenix Pharmaceuticals), anti-hypocretin (1:4000 ...
-
No products found
because this supplier's products are not listed.
Dhruv Raina, et al.,
bioRxiv - Developmental Biology 2020
Quote:
... prepared as a 1:1 mixture of DMEM/F12 (PAN Biotech) and Neuropan basal medium (PAN Biotech ...
-
No products found
because this supplier's products are not listed.
Tamara Phan, et al.,
bioRxiv - Cell Biology 2020
Quote:
... 1:50 cOmplete) by a Focused-ultrasonicator (Covaris M220, 1 ml) to yield chromatin fragments with an average size of 600 bp.