Quote:
... Immunocomplexes were visualized using the ECL substrates kits from Cyanagen and imaged on ALLIANCE MINI HD9 system (UVITEC Ltd, UK). Quantification of bands was performed using the Uvitec Nine Alliance Software ...
Laura Ohl, et al.,bioRxiv - Molecular Biology 2023
Quote:
... Tissue was mixed with 1 mL 40:40:20 methanol:acetonitrile:water (extraction solvent) and homogenized with a benchtop lyser (SciLogex OS20-S) at 1600 mhZ for approximately 15 sec ...
Elizabeth V. K. Ledger, Andrew M. Edwards,bioRxiv - Microbiology 2023
Quote:
... or C3 was detected with a 1:1000 dilution of goat anti-human C3 F(ab’)2 labelled with FITC (Protos Immunoresearch). Antibody incubations were carried out statically for 1 h at room temperature in the dark ...
Kevin M. Tharp, et al.,bioRxiv - Cell Biology 2020
Quote:
... 1 μL of sample was separated over a 25 cm column packed with 1.9 μm Reprosil C18 particles (Dr. Maisch HPLC GmbH) by a nanoElute HPLC (Bruker) ...
Farhad S. Golzar, et al.,bioRxiv - Evolutionary Biology 2021
Quote:
... Genome quality and quantity were checked in 1% agarose gels stained with %0.0001 SYBR Safe and measured in a NanoDrop (ACTGene ASP-3700, Alphatech Systems), respectively ...
Quote:
... was homogenised for 30 seconds in 90 mL of sterile 1× phosphate buffered saline (PBS) using a BagMixer® 400 P laboratory blender (Interscience, Edwards Group). This was repeated two more times to obtain a total of three replicates per pool sample ...
Ezekiel C. Thomas, Amber Ismael, Jeffrey K. Moore,bioRxiv - Cell Biology 2020
Quote:
... and 1% yeast extract) at 30°C then diluted into synthetic media (2% glucose, CSM from Sunrise Science Products, #1001 San Francisco, CA) and grown to log phase at 30°C ...
Kathleen L. Arnolds, et al.,bioRxiv - Microbiology 2023
Quote:
Mesocosm experiments were conducted in 3D-printed pots (S. Fig. 1) that have 18 sampling ports which are sealed with neoprene stoppers (Eisco Labs, Victor, NY). A perforated base allows for root development ...