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... bipolar EMG from the TA was recorded with two surface Ag-AgCl electrodes 2 cm apart (WhiteSensor 40713, Ambu). The ground electrode was placed on the right ankle ...
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... for 2–4 h and fluorescence (560Ex/590Em) was measured in a microplate reader (TriStar LB 941, Berthold Technologies). To monitor cell growth of RPE1 cells ...
Neil J Rzechorzek, et al.,bioRxiv - Biochemistry 2020
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... HEK293-F cells were grown in 400 mL batches in 2 L non-baffled Erlenmeyer flasks (TriForest Enterprises Inc, #FPC2000S) to a cell density of ∼1×106 cells/mL.
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... cells were washed and cryopreserved in a 1:1 mixture of CS10 (BioLife Solutions) and Plasma-Lyte A (Hanna Pharmaceutical) supplemented with 2% HSA (Access Biologicals). Cryopreserved cells were thawed and activated with anti-CD3/anti-CD28 TransAct (Miltenyi ...
Marine Zagdoun, et al.,bioRxiv - Microbiology 2019
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... adapted as follows: 20 g of meat were homogenized for 2 × 30 s in a stomacher bag (BagPage, Interscience, Saint-Nom-la-Bretèche, France) with 40 mL of sterile ultrapure water supplemented with 1% Tween 80 (Acros Organics ...
Nada Sallam, et al.,bioRxiv - Pharmacology and Toxicology 2023
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... or weighed frozen (hippocampus or adipose tissue) and placed into glass tubes with 2 mL acetonitrile and 100 μL deuterated internal standard (Cerilliant, Round Rock, TX, USA). Hippocampal or adipose samples were first homogenised with a glass rod ...
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The following protocol was adapted from the μMACS Streptavidin Kit protocol (Miltenyi): 1μg of biotinylated antibodies to CD26 or CD36 (Ancell) were added to 1ml of virus ...
Arundhasa Chandrabalan, et al.,bioRxiv - Pharmacology and Toxicology 2020
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... All joint fluids were tested for presence of any blood with the Fecal Occult Blood Test kit (Immunostics Hema-Screen) and the samples showing presence of blood were excluded from further analysis.
Christopher A. Hempel, et al.,bioRxiv - Genomics 2022
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DNA and total RNA were separately extracted from samples in 96-well plates using the NucleoMag DNA/RNA Water kit (D-MARK Biosciences, Toronto, Canada) that includes magnetic beads ...