Mark A. Skylar-Scott, et al.,bioRxiv - Bioengineering 2020
Quote:
Wild type PGP1-iPSCs were dissociated using EDR and seeded into anti-adhesive treated V-bottom 96-well plates (Globe Scientific, #120130) at a density of 1,000 cells/96-well well in EBCM + 10 μM Y-27632 for 12 h to generate organoid cores.
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... LaG-16 anti-GFP nanobodies32 were recombinantly produced in house and labeled with α-Biotin-ω-(succinimidyl propionate) 24(ethylene glycol) (Iris Biotech) as previously described33,34 ...