-
No products found
because this supplier's products are not listed.
Esther Wortmann, et al.,
bioRxiv - Microbiology 2024
Quote:
... mRNA concentrations were determined using a Nanodrop (Peqlab VWR, USA) and 20 ng were used for reverse transcription using the RevertAid H Minus First Strand cDNA Synthesis kit (ThermoFisher Scientific ...
-
No products found
because this supplier's products are not listed.
A.S. Gross, et al.,
bioRxiv - Cell Biology 2022
Quote:
... α-mCherry (polyclonal, Genetex), α-Atg13 (polyclonal ...
-
No products found
because this supplier's products are not listed.
Maria Inês Alvelos, et al.,
bioRxiv - Systems Biology 2021
Quote:
... mRNA was reverse transcribed using the Reverse Transcriptase Core kit (Eurogentec). In order to reduce variability in mRNA input ...
-
No products found
because this supplier's products are not listed.
Aki Teranishi, et al.,
bioRxiv - Developmental Biology 2024
Quote:
... The lentivirus was produced by co-transfection of the expression vector coding Lifeact-mCherry and lentiviral packaging plasmids (pCMVdR8.91 and pMD2.G) using PEI MAX (Polysciences #24765) into HEK293T cells ...
-
No products found
because this supplier's products are not listed.
Kalliopi Skamaki, et al.,
bioRxiv - Biochemistry 2020
Quote:
scFv-ribosome-mRNA complexes were subjected to selection in solution using recombinant biotinylated human IL-13 (Peprotech) to allow capture using streptavidin-coated paramagnetic beads (Dynabeads M-280) ...
-
No products found
because this supplier's products are not listed.
Prasun Kundu, et al.,
bioRxiv - Microbiology 2022
Quote:
Purified biotinylated proteins were multimerised on streptavidin coated Nile Red fluorescent beads (Spherotech Inc ...
-
No products found
because this supplier's products are not listed.
David Allard, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... Proteins were revealed with fluorescent secondary anti-rabbit or anti-mouse antibodies (1:10 000; LI-COR) using the LI-COR fluorescent scanner or by chemiluminescence (Bio-Rad ...
-
No products found
because this supplier's products are not listed.
Maria Fernanda Fernandes, et al.,
bioRxiv - Pharmacology and Toxicology 2022
Quote:
... and M protein (YP_009724393.1) tagged at the C-terminus with green fluorescent protein (Ex-NV225-M03) were from GeneCopoeia (Rockland, MD, U.S.A). The control plasmid was pCMV-3Tag-3A (pCMV ...
-
No products found
because this supplier's products are not listed.
Olya Mastikhina, et al.,
bioRxiv - Bioengineering 2019
Quote:
... CATS mRNA-seq kit (with polyA selection) (Diagenode, Cat No. C05010051) was used ...
-
No products found
because this supplier's products are not listed.
Federico Cocozza, et al.,
bioRxiv - Cell Biology 2023
Quote:
... of the EVs and ENPs from EO771 m/p-mCherry or controls (medium alone, recombinant mCherry, LPS-EB ultrapure 10 µg/ml (Invivogen) and CpG 2 µg/ml (TriLink ...
-
No products found
because this supplier's products are not listed.
Hooman Bagheri, et al.,
bioRxiv - Genetics 2020
Quote:
... M4KO mice were generated by cytoplasmic microinjection of 25ng/ul Cas9 mRNA (PNA Bio) and 12.5ng/ul of each of four sgRNAs ...
-
No products found
because this supplier's products are not listed.
Adeline Arini, et al.,
bioRxiv - Pharmacology and Toxicology 2022
Quote:
... The mRNA concentration was measured in a Take3 plate with a microplate spectrometer (BioTek EPOCH). The RNA purity was checked by comparing the ratio between values measured at 260 and 280 nm and ensured when the ratio was over 2 ...
-
No products found
because this supplier's products are not listed.
Julian Lutze, Donald Wolfgeher, Stephen J. Kron,
bioRxiv - Cancer Biology 2021
Quote:
... fluorescent secondary antibodies (Jackson ImmunoResearch) were applied for 1 h at RT ...
-
No products found
because this supplier's products are not listed.
Savanna-Rae H. Fahoum, Dawn M. Blitz,
bioRxiv - Neuroscience 2021
Quote:
... Using a Model 1700 A-M Systems Amplifier (A-M Systems, Carlsborg, WA), extracellular activity in nerves were recorded using custom-made stainless steel pin electrodes ...
-
No products found
because this supplier's products are not listed.
Emily J. Sherman, Brian T. Emmer,
bioRxiv - Microbiology 2021
Quote:
... of the human ACE2 coding sequence (Sino Biological, Beijing China, #HG10108-M) into LeGO-iC2 (Addgene #27345 ...
-
No products found
because this supplier's products are not listed.
Tao Wang, et al.,
bioRxiv - Neuroscience 2022
Quote:
Capped mRNAs were pulled down by m7GTP-sepharose (Jena Bioscience) as described with modifications 44 ...
-
No products found
because this supplier's products are not listed.
Stéphane Supplisson,
bioRxiv - Neuroscience 2024
Quote:
... and GluN2A mRNAs using a nanoliter injector (World Precision Instruments). Post-injection ...
-
No products found
because this supplier's products are not listed.
Fu-Qing Jiang, et al.,
bioRxiv - Cell Biology 2022
Quote:
... The fragmented mRNA was incubated with anti-m6A antibody (Synaptic Systems), anti-Ythdf2 antibody (Proteintech) ...
-
No products found
because this supplier's products are not listed.
Xingkun Jin, et al.,
bioRxiv - Immunology 2020
Quote:
... Target mRNAs were amplified using MyTaq HS DNA Polymerases (Bioline, UK), and amplicons were run on 1% agarose gels ...
-
No products found
because this supplier's products are not listed.
Nima Aliakbarinodehi, et al.,
bioRxiv - Biophysics 2024
Quote:
... mRNA or PolyA were diluted in RNase free citrate buffer (TekNova) of 50 mM at pH 3.0 to obtain a mRNA/PolyA:lipid weight ratio of 10:1 ...
-
No products found
because this supplier's products are not listed.
Timothy Erickson, et al.,
bioRxiv - Cell Biology 2023
Quote:
... Biotinylation of the mRNA was done using the MTSEA-biotin-XX reagent (Biotium 90066). A 5 mg/ml solution was prepared in N,NDimethylformamide (DMF ...
-
No products found
because this supplier's products are not listed.
Hsu-Yu Chen, et al.,
bioRxiv - Microbiology 2021
Quote:
... VSV M protein was detected using anti-VSV M antibody clone [23H12] (KeraFast, Boston, MA, Cat.# EB0011) at 1:1000 ...
-
No products found
because this supplier's products are not listed.
Xianpeng Ge, et al.,
bioRxiv - Cell Biology 2020
Quote:
Recombinant Mouse M-CSF Protein (cat# 461-ML, R&D Systems)
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No products found
because this supplier's products are not listed.
A. Gallud, et al.,
bioRxiv - Biophysics 2021
Quote:
... The LNPs were detected by Cy5 mRNA emission using an F46- 006 ET filter set (Chroma Technology GmbH, Olching, Germany). The recorded data was analysed using a custom-written script in MATLAB R2017B (MathWorks ...
-
No products found
because this supplier's products are not listed.
O Diana Sáenz-de la, et al.,
bioRxiv - Plant Biology 2021
Quote:
... Accumulation of mRNA transcripts for marker genes involved in UV-B signaling or responding to UV-B (UVR8; BRI1-EMS-SUPPRESSOR 1 ...
-
No products found
because this supplier's products are not listed.
Bonita H. Powell, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... RPL26 1:1000 dilution (Bethyl A300-686A-M Lot#A300-686A-M-1), Actin 1:10,000 dilution (Sigma A1978-100μL Lot#065M4837V) ...
-
No products found
because this supplier's products are not listed.
Carrie E. Leonard, et al.,
bioRxiv - Neuroscience 2020
Quote:
... mRNA puncta were quantified using Imaris software (Bitplane, Oxford Instruments). For cortical culture ...
-
No products found
because this supplier's products are not listed.
Xu Peng, et al.,
bioRxiv - Synthetic Biology 2023
Quote:
... The capped mRNA underwent further purification through oligo-dT chromatography (Sartorius) before being dissolved in 10 mM Citrate buffer at pH 4.0 ...
-
No products found
because this supplier's products are not listed.
Ryan M. Carr, et al.,
bioRxiv - Cancer Biology 2019
Quote:
... incubated in protein block (Rodent Block M, Biocare) for 30 minutes ...
-
No products found
because this supplier's products are not listed.
Jingyi Yang, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... Sequencing libraries were generated using mRNA-seq Lib Prep Kit for Illumina (ABclonal, USA, Cat. RK20302) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample ...
-
No products found
because this supplier's products are not listed.
Anna Lipońska, et al.,
bioRxiv - Microbiology 2024
Quote:
... The translation of the reporter mRNA was monitored every 2 min by measuring YFP fluorescence was measured on a CLARIOstar plate reader (BMG Labtech).
-
No products found
because this supplier's products are not listed.
Yukinori Ikeda, et al.,
bioRxiv - Cancer Biology 2024
Quote:
... CD31 or fluorescent proteins were stained with VECTOR ImmPACT DAB peroxidase substrate kit (Vector Laboratories) or VECTOR red alkaline phosphatase substrate kit (Vector Laboratories) ...
-
No products found
because this supplier's products are not listed.
Agata Casado-Sainz, et al.,
bioRxiv - Neuroscience 2021
Quote:
... (7 mDS-mCherry, 4F/3M)) were fasted overnight before the PET scan ...
-
No products found
because this supplier's products are not listed.
Zhuo Chen, et al.,
bioRxiv - Bioengineering 2019
Quote:
... Protein signal data were extracted from the multicolor fluorescent images using GenePix Pro 6.1 (Molecular Devices) by aligning a microtrough array template with feature blocks per antibody per microtrough to the protein signal features ...
-
No products found
because this supplier's products are not listed.
Sara Faccidomo, et al.,
bioRxiv - Neuroscience 2021
Quote:
... fluorescent retrobeads (Lumafluor) were injected bilaterally in the NAcbC (0.3 µl/injection) ...
-
No products found
because this supplier's products are not listed.
Veronika Thallmair, et al.,
bioRxiv - Cell Biology 2020
Quote:
Oxotremorin-M (Oxo-M, Tocris Bioscience, Bristol, UK) was prepared in a 10mM stock solution in water and diluted to 10 µM in extracellular solution ...
-
No products found
because this supplier's products are not listed.
Fadil M. Hannan, et al.,
bioRxiv - Genetics 2020
Quote:
... Cas9 mRNA (Tebu-Bio), sgRNA and ssODN were diluted and mixed in microinjection buffer (MIB ...
-
No products found
because this supplier's products are not listed.
Helena Escobar, et al.,
bioRxiv - Genetics 2024
Quote:
... 2.5 µg of SpCas9 mRNA (Aldevron) plus 1.7 µg of 5’/3’-end-modified sgRNA (IDT or Synthego ...
-
No products found
because this supplier's products are not listed.
Vanessa Barone, Maria Byrne, Deirdre C. Lyons,
bioRxiv - Developmental Biology 2022
Quote:
Lytechinus pictus embryos were injected with mRNAs coding for membrane and nuclear markers on a glass bottom dish (MatTek, P35G-1.5-14-C) coated with protamine ...
-
No products found
because this supplier's products are not listed.
Aya Al Othman, et al.,
bioRxiv - Cancer Biology 2023
Quote:
Binding affinity of Arc protein to mRNA was measured using a bio-layer interferometer BLItz Pro (ForteBio, Pall Life Sciences, NY). Biotinylated mRNAs (Supplementary Methods) ...
-
No products found
because this supplier's products are not listed.
Lauren E. Closs, et al.,
bioRxiv - Zoology 2023
Quote:
... Total pituitary mRNA was extracted by homogenizing pituitaries in Trizol with 6 lysing beads (MP Biomedicals) with a tabletop vortex ...
-
No products found
because this supplier's products are not listed.
Kuo-Sheng Lee, et al.,
bioRxiv - Neuroscience 2023
Quote:
Temporal coding index (TCI) and rate coding index (RCI ...
-
No products found
because this supplier's products are not listed.
Wei Zhang, et al.,
bioRxiv - Neuroscience 2023
Quote:
... chicken anti-mCherry (Aves Labs, Cat# MCHERRY-0020), rabbit anti-Flag conjugated with HRP (Cell Signaling ...
-
No products found
because this supplier's products are not listed.
Michaela A. Edmond, et al.,
bioRxiv - Biophysics 2022
Quote:
... mRNA (40-50nL) was injected into Xenopus leavis oocytes (purchased from Ecocyte) using a NanojectII nanoinjector (Drummond Scientific) and electrophysiological experiments were performed 2 to 5 days after injection.
-
No products found
because this supplier's products are not listed.
Luis Alberto Bezares Calderón, et al.,
bioRxiv - Animal Behavior and Cognition 2023
Quote:
... see Microinjection and generation of plasmid constructs and mRNA section) were embedded in ∼2.7% low-melting agarose (HR8-092, Hampton Research) with an anterior to posterior orientation and placed on a ø25 mm coverslip (CB00250RAC33MNZ0 ...
-
No products found
because this supplier's products are not listed.
Alexander Beatty, et al.,
bioRxiv - Cancer Biology 2019
Quote:
... Efficiency of mRNA depletion was assessed 72 hours post-transfection using qPCR or western blot in the case of ACSL1 (Proteintech, 13989-1-AP). In experiments to determine suppression of cell toxicity resulting from αESA or ML162 by silencing expression of individual ACSL or ALOX genes ...
-
No products found
because this supplier's products are not listed.
Ahmed Abu-Zaid, et al.,
bioRxiv - Cancer Biology 2019
Quote:
KDM4B was labeled with a cysteine reactive fluorescent dye (Protein Labeling Kit RED-MALEIMIDE, Nanotemper GmbH). A serial dilution of titrant was prepared in MST buffer containing 10 mM HEPES (pH 7.5) ...
-
No products found
because this supplier's products are not listed.
Jonathan Yaacov Weinstein, et al.,
bioRxiv - Biochemistry 2023
Quote:
... and 3µl 10% APS and 57µl fluorescent protein in PBS) inside 8-well microscope slides (ibidi No. 80826). Slides were mounted to Eclipse TI-E Nikon inverted microscope (Nikon Instruments Inc. ...
-
No products found
because this supplier's products are not listed.
Saray Gutiérrez, et al.,
bioRxiv - Microbiology 2021
Quote:
... red fluorescent beads (Bangs Laboratories) were coated with 5-Dodecanoylaminofluorescein Di-β-D-Galactopyranoside (C12FDG ...
-
No products found
because this supplier's products are not listed.
Jesús Ordoño, et al.,
bioRxiv - Bioengineering 2020
Quote:
... Acti-stain™ Fluorescent Phalloidin (Cytoskeleton, Inc.) and DAPI (Sigma ...