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Citations for World Precision Instruments :
1 - 32 of 32 citations for M CHERRY MRNA mCherry Fluorescent Protein coding mRNA since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... and GluN2A mRNAs using a nanoliter injector (World Precision Instruments). Post-injection ...
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bioRxiv - Developmental Biology 2020Quote: ... One-cell-stage AB WT embryos were injected with gRNA and Ca9 mRNA with a Picopump (World Precision Instruments). Potential founders (F0 ...
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bioRxiv - Developmental Biology 2019Quote: ... A total of 1-5 nl mRNAs were microinjected into one-cell stage wild-type zebrafish embryo using the WPI microinjector (WPI, USA). The 1-2 dpf embryos were anesthetized in 0.016% tricaine and photographed under a Leica stereomicroscope.
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bioRxiv - Biophysics 2022Quote: ... WT and mutant rP2X2R mRNAs (concentration 50 - 3000 ng μl−1) were injected into the oocyte cytoplasm with a Nanoliter 2010 injector (World Precision Instruments). The volume of mRNA injected varied depending on the construct (10-50 nl) ...
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bioRxiv - Genetics 2021Quote: ... 50 to 100 one-cell stage WT embryos were injected with the gRNA (150pg per embryo) and Cas9 mRNA (100pg per embryo) with a Picopump (World Precision Instruments). Strong gRNAs were identified by isolating genomic DNA from 24 individual injected embryos and 3 uninjected embryos at 3-5 dpf ...
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bioRxiv - Neuroscience 2021Quote: ... Both barrels were filled with carbogenated ACSF containing 5 μM of the fluorescent dye Alexa 594 and connected to the positive and negative poles of an isolated stimulator (WPI, Getting Instruments or ISO-Flex). The pipette tip was positioned under visual control in close proximity (within 2 μm ...
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bioRxiv - Neuroscience 2020Quote: ... The craniotomy after a hM4Di or mCherry injection was covered with silicone elastomer (Kwik-Cast, WPI) and dental cement ...
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bioRxiv - Neuroscience 2019Quote: ... filled with 1 M HRP in 1 M NaCl and a resistance of 7 to 10 MΩ (measured with Ωmega-Tip Z, World Precision Instruments Inc., USA). The electrode was mounted on a piezo drive (Inchworm controller 8200 ...
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bioRxiv - Neuroscience 2021Quote: ... The implanted GRIN lens was secured using 3 M Vetbond (WPI) on the skull ...
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bioRxiv - Developmental Biology 2022Quote: ... M-152 micromanipulators (Narishige) and 1B100f-4 capillaries (World Precision Instruments) shaped with P-1000 micropipette puller (Sutter Instrument Company).
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bioRxiv - Neuroscience 2023Quote: ... and a sample tube containing a fluorescent polymer (World Precision Instruments, cat # KWIK-CAST) was placed at the tip of patch cable ...
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bioRxiv - Neuroscience 2022Quote: ... We injected the construct AAV2-CamKIIa-hChR2(H134R)-mCherry (1 μl, UNC Vector Core) unilaterally using a microsyringe (34 GA. beveled Needle, 10 μ l NANOFIL syringe, WPI) into the left LGN (AP 3.1 ...
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bioRxiv - Neuroscience 2023Quote: ... pAAV-hSyn-DIO-hM3D(Gq)-mCherry or pAAV-hSyn-DIO-hM4D(Gi)-mCherry were injected at a rate of 100 nl/min using an injector (Nanoliter 2010, WPI, FL, USA) attached to a pulled glass injection micropipette (∼50 µm tip ...
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bioRxiv - Neuroscience 2022Quote: ... Recording pipettes (2.0-2.2 M□ resistance) were constructed from thin wall capillaries (World Precision Instruments) with a PC-10 puller (Narishige International) ...
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bioRxiv - Cell Biology 2022Quote: ... ZMEL-GFP cells and ZMEL-mCherry cells that expressing WT-Paxillin were plated on either glass bottom dishes (World Precision Instruments, FD35-100), or collagen coated dishes (Matrigen ...
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bioRxiv - Neuroscience 2021Quote: ... 0.05 M PBS) was infused at a rate of 100 nl/min (UMP3 electrical pump, WPI). Once the full viral injection was delivered the injection needle was left in place for 10 minutes to prevent backflow of the injected virus solution.
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bioRxiv - Neuroscience 2023Quote: ... The virus (AAV1/2-OXTp-hM4Dgi-mCherry or AAV1/2-OXTp-Venus or AAV1/2-OXTp-mCherry) was loaded into a 20μl NanoFil syringe fitted with a 33gauge needle (cat no. NF33BL, World Precision Instruments Inc, Sarasota, FL, USA). A final volume of 270nl was injected into the PVH (A-P −1.7mm ...
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bioRxiv - Developmental Biology 2022Quote: ... The embryos were aged to cycle 9-10 before injection of fluorescent probes using a needle equipped on a manual micromanipulator (WPI) and controlled by the SYS-PV830 pneumatic pump (WPI) ...
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bioRxiv - Neuroscience 2021Quote: ... we pulled 6 to 10 M-Ohm glass pipettes made of thick-walled glass (World Precision Instruments 1B150F-3) using a Sutter Instruments P-1000 puller ...
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bioRxiv - Neuroscience 2019Quote: ... The C-fibers were classified according to their conduction velocity of less than 1.2 m/s measured by electrical stimulation (Stimulus Isolator A385, WPI). The skin receptive field of C-fibers was isolated from the surrounding bath chamber with a stainless steel ring 0.8 cm in diameter ...
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bioRxiv - Cell Biology 2023Quote: ... +2.5 mm L/M and −3.0 mm D/V using pulled borosilicate glass micropipettes (WPI, Sarasota, FL, #1B100-4). Glass micropipettes were mounted to the stereotaxic frame via specialized connectors and attached ...
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bioRxiv - Neuroscience 2020Quote: ... dissolved in 0.1 M sodium PBS, pH 7.4) was delivered into the CeA (500nL per hemisphere, 100nL/min) using UMP3 UltraMicroPump (World Precision Instruments) and the syringe remained in place for another 5 min to allow for the diffusion of the tracer ...
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bioRxiv - Synthetic Biology 2020Quote: ... lyophilized peptides were dissolved in Milli-Q H2O to a concentration of 750 µM and 18 nL of solubilized peptide was injected into cRNA pre-injected oocytes with the Nanoliter 2010 micromanipulator (World Precision Instruments). For NaV1.5 constructs ...
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bioRxiv - Neuroscience 2022Quote: ... They were filled with a 1 M KCl solution using a MicroFil non-metallic syringe needle (MF 34G-5, World Precision Instruments); a few recordings were done with 3 M KCl (no particular change was noticed).
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bioRxiv - Neuroscience 2023Quote: ... Animals were injected into their foot with 3-5 µL of 10-5 M ELH using a 10 µL Nanofil syringe (World Precision Instruments) equipped with a 35-gauge needle ...
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bioRxiv - Neuroscience 2020Quote: ... individual 7 µm diameter carbon fibres (Goodfellow Cambridge Limited, Cambridge, UK) were bundled and introduced into a pulled double borosilicate capillary (WPI, ref. TST150-6”) with ≃ 200 µm tip diameter and electrical impedance of 40-65 kΩ ...
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bioRxiv - Plant Biology 2023Quote: Proteins were isolated from six leaf discs (5mm Ø World Precision Instruments (WPI)) ...
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bioRxiv - Plant Biology 2023Quote: Proteins were isolated from six leaf discs (5mm Ø World Precision Instruments (WPI)) ...
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bioRxiv - Biophysics 2022Quote: ... A micropipette was filled with fluorescently labeled protein using a MicroFil flexible needle (MF34G-5, World Precision Instruments, UK). The micropipettes were introduced in each side of the chamber and placed in the middle using micromanipulators ...
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bioRxiv - Biophysics 2021Quote: ... A micropipette was filled with the same buffer as the protein (20 mM Tris and 150 mM NaCl, pH 7.5) using a MICROFIL needle (WPI) and subsequently mounted onto a micromanipulator ...
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bioRxiv - Developmental Biology 2020Quote: ... A solution containing 25 ng/µL gRNA and 100 ng/µL Cas9 mRNA or 1000 ng/µL Cas9 protein (Toolgen) was injected into one-cell-stage embryos using a pneumatic microinjector (PV830, WPI). The insertion and/or deletion (indel ...
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bioRxiv - Developmental Biology 2023Quote: ... A solution containing 25 ng/μL gRNA and 100 ng/μL Cas9 mRNA or 1000 ng/μL Cas9 protein (ToolGen Inc.) was injected into one-cell-stage embryos using a pneumatic microinjector (PV830, WPI). To establish the foxp1b mutant ...