-
No products found
because this supplier's products are not listed.
Matteo Lunghi, et al.,
bioRxiv - Microbiology 2021
Quote:
... and trimethylsilylated shortly before analysis through addition of 20 μl N,O-bis(trimethylsilyl)trifluoroacetamide with 1 % trimethylchlorosilane (BSTFA-TMCS, Cerilliant, Sigma B-023). The GC-MS instrumentation and settings (electron ionization mode ...
-
No products found
because this supplier's products are not listed.
Roberto Balbontín, Nelson Frazão, Isabel Gordo,
bioRxiv - Microbiology 2020
Quote:
... In the competitions including the RNase HI inhibitor 2-[[[3-Bromo-5-(2-furanyl)-7-(trifluoromethyl)pyrazolo[1,5-a]pyrimidin-2-yl]carbonyl]amino]-4,5,6,7-tetrahydro-benzo[b]thiophene-3-carboxylic acid ethyl ester (RHI001, Glixx Laboratories Inc., catalog number GLXC-03982), the medium was supplemented with 500 µM of RHI001 ...
-
No products found
because this supplier's products are not listed.
Na Wei, Jiaqi Tan,
bioRxiv - Microbiology 2023
Quote:
... and total dissolved solids (TDS) of the aquatic environment at each population using an Ohaus ST20M-B meter (Ohaus Corporation, Parsippany, New Jersey). Additionally ...
-
No products found
because this supplier's products are not listed.
Michael Bugiel, et al.,
bioRxiv - Biophysics 2020
Quote:
... surrounded by monofunctional PEG molecules (mPEG, 2 kDa, Rapp Polymere, Tübingen, Germany) in a ratio of 1 ...
-
No products found
because this supplier's products are not listed.
Heather Swann, et al.,
bioRxiv - Biophysics 2020
Quote:
... followed by a brief dd-H20 wash to get rid of excess neutravidin and incubation with biotinylated anti-S antibody (11-2001-B, Abeomics, San Diego, CA, USA). Surfaces thus prepared were generally devoid of debris but sometimes had step-edge character suggesting that antibody coating was less than a full monolayer (Figure S4) ...
-
No products found
because this supplier's products are not listed.
Jonas Goretzko, et al.,
bioRxiv - Immunology 2021
Quote:
... PMN rolling and adhesion were determined using a parallel plate flow chamber assembly (Glycotech, Gaithersburg, MD, USA) perfused with isolated PMNs (2×105 cells/mL ...
-
No products found
because this supplier's products are not listed.
Bradley E. Poulsen, et al.,
bioRxiv - Microbiology 2024
Quote:
... 2D NOESY (Nuclear Overhauser Effect Spectroscopy) of the molecule available from Vitas-M showed correlation peaks between the imidazolone methylene and two phenyl hydrogens ...
-
No products found
because this supplier's products are not listed.
Luana dos Santos Ortolan, et al.,
bioRxiv - Pathology 2019
Quote:
... and the slides were added to a flow system composed of a chamber (cell adhesion flow chamber, Immunetics) that kept the slide adhered through the formation of a vacuum (coupled by a vacuum pump) ...
-
No products found
because this supplier's products are not listed.
Vivian K. Rojas, et al.,
bioRxiv - Microbiology 2024
Quote:
... The chromogenic molecule X-Phos (5-bromo-4-chloro-3-indolyl phosphate, Chem-Impex) was added to agar plates with the purpose of detecting S ...
-
No products found
because this supplier's products are not listed.
Doris Krauter, et al.,
bioRxiv - Neuroscience 2021
Quote:
... using a diamond knife (Histo HI 4317, Diatome). Afterwards sections were stained according to Gallyas52 and with Methylene blue/ Azur II for 1 min ...
-
No products found
because this supplier's products are not listed.
Thomas D. Avery, et al.,
bioRxiv - Pharmacology and Toxicology 2021
Quote:
NRF2/ARE luciferase reporter HEK293 cells (SL-0042-NP, Signosis, Santa Clara, USA) were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM ...
-
No products found
because this supplier's products are not listed.
Krzysztof Mikolajczyk, et al.,
bioRxiv - Biochemistry 2021
Quote:
... and thus allow plotting calibration curves (mean fluorescence intensity versus Molecules of Equivalent Soluble Fluorochrome units). The cells were then analyzed by flow cytometry and the antibody binding capacity (ABC ...
-
No products found
because this supplier's products are not listed.
Chafen Lu, et al.,
bioRxiv - Microbiology 2019
Quote:
... Secondary antibodies were rabbit polyclonal anti-His (Delta Biolabs), Horseradish peroxidase (HRP)-anti-rabbit and HRP-anti-mouse IgG (GE Healthcare ...
-
No products found
because this supplier's products are not listed.
Benjamin Tam, et al.,
bioRxiv - Biochemistry 2019
Quote:
In the initial screening 10 fragment molecules from the Maybridge Ro3 2500 Diversity Fragment Library (Maybridge, Inc.) were pooled ...
-
No products found
because this supplier's products are not listed.
Carina C D Joe, et al.,
bioRxiv - Bioengineering 2021
Quote:
Residual host-cell protein (HCP) was quantified using the HEK293 HCP ELISA kit (Cygnus Technologies) according to the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Laura Sánchez-Caballero, et al.,
bioRxiv - Cell Biology 2019
Quote:
... HEK293 cells expressing inducible NDUFAF3-GFP were cultured in a Wilco dish (Intracel, Royston, UK), washed with phosphate-buffered saline (PBS) ...
-
No products found
because this supplier's products are not listed.
Hao-Ching Jiang, et al.,
bioRxiv - Animal Behavior and Cognition 2023
Quote:
... HEK293 cells were plated on 12 mm round German glass coverslips (Bellco Biotechnology, #1943-10012A) coated with poly-d-lysine hydrobromide (Sigma-Aldrich ...
-
No products found
because this supplier's products are not listed.
Aanandita Kothurkar, et al.,
bioRxiv - Developmental Biology 2024
Quote:
... and frozen at −80°C. SuperFrost™ Plus Adhesion Microscope Slides (Epredia, Cat. No. J1800AMNZ) were coated evenly with 5 μL chrome alum gelatin (Newcomer Supply, Part# 1033C) and dried in an incubator at 60°C for one hour ...
-
No products found
because this supplier's products are not listed.
Yunming Wu, et al.,
bioRxiv - Bioengineering 2021
Quote:
... was used for gel electrophoresis analysis DNA molecules were stained with Midori Green Direct DNA staining dye (Bulldog Bio) for gel loading ...
-
No products found
because this supplier's products are not listed.
Eric M. Rosenberg Jr., et al.,
bioRxiv - Biochemistry 2023
Quote:
... Initial screens were performed with the protein pre-incubated with small molecule ligands using a Mosquito system (TTP Labtech) and several commercial crystal screens from Hampton Research ...
-
No products found
because this supplier's products are not listed.
Chao Li, et al.,
bioRxiv - Bioengineering 2024
Quote:
... Surface modification introduces a monolayer of covalently bonded PDMS-silane (1,3-dichlorotetramethylsiloxane, Gelest, SID3372.0) molecules by vacuum chemical vapor deposition (CVD) (Bel-Art F420220000 ...
-
No products found
because this supplier's products are not listed.
Ole A.W. Haabeth, et al.,
bioRxiv - Immunology 2021
Quote:
... 2 μg of the RBD-his mRNA or GFP mRNA (Trilink) was formulated in 6.6 μl of PBS pH 5.5 with 1.37 μl of 5mM CART and added to the cells ...
-
No products found
because this supplier's products are not listed.
Lorna Ewart, et al.,
bioRxiv - Pharmacology and Toxicology 2022
Quote:
... Cryopreserved human Kupffer cells (Samsara Sciences) and human Stellate cells (IXCells) were thawed according to their respective vendor/Emulate protocols on the day of seeding ...
-
No products found
because this supplier's products are not listed.
Camden R. Bair, et al.,
bioRxiv - Microbiology 2021
Quote:
... supplemented with 8% heat-inactivated newborn calf serum (HI-NBCS, Rocky Mountain Biologicals), 1.5 g/ml Na2CO3 (Amresco) ...
-
No products found
because this supplier's products are not listed.
Mathilde Ambrosino, et al.,
bioRxiv - Bioengineering 2023
Quote:
... The concentrations of released fluorescent molecules and cytokines in the supernatants were quantified using either a microplate reader for fluorescent measurements (Tristar 2S, Berthold Technologies) or ELISA kits (R&D Systems ...
-
No products found
because this supplier's products are not listed.
Neil J Rzechorzek, et al.,
bioRxiv - Biochemistry 2020
Quote:
... HEK293-F cells were grown in 400 mL batches in 2 L non-baffled Erlenmeyer flasks (TriForest Enterprises Inc, #FPC2000S) to a cell density of ∼1×106 cells/mL.
-
No products found
because this supplier's products are not listed.
Justine Creff, et al.,
bioRxiv - Cell Biology 2022
Quote:
... 200 µg for HEK293) were incubated with 3 µg of the indicated antibodies and 12 µL protein sepharose beads (IPA300, Repligen) at 4°C for 4 h ...
-
No products found
because this supplier's products are not listed.
Anne Rehkamp, et al.,
bioRxiv - Biochemistry 2020
Quote:
LC separation of peptides originating from HEK293 cell membranes was performed via selfpacked C18 columns (PicoFrits, 75 μm x 50 cm, 15 μm tip, New Objective, self-packed with ReproSil-Pur 120 C18-AQ ...
-
No products found
because this supplier's products are not listed.
Ronan Shaughnessy, et al.,
bioRxiv - Cell Biology 2023
Quote:
... lentivi-ral particles were produced in the HEK293 FT packaging cells transfected with MiniBAR constructs and the Excelenti LTX Lentivirus Packaging mix (Oxford Genetics) using lipo-fectamine following manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Andrew R. McEwan, et al.,
bioRxiv - Genetics 2021
Quote:
... from human DNA (Cambio, UK) using the following primers ...
-
No products found
because this supplier's products are not listed.
Elena Arutyunova, et al.,
bioRxiv - Biophysics 2021
Quote:
... The fusion protein was subsequently digested with His-tagged SUMO protease (McLab, South San Francisco, CA) at 4 °C for 1–2 h to remove the SUMO tag and the resulting cleavage mixture was then passed through Ni-NTA resin column ...
-
No products found
because this supplier's products are not listed.
Aman Y. Husbands, et al.,
bioRxiv - Plant Biology 2022
Quote:
... Membranes were washed three times with PBS-T and incubated with 1:2000 anti-His-tag antibody (Abiocode M0335-1) for 1h with gentle shaking at RT ...
-
No products found
because this supplier's products are not listed.
Cunxi Wang, et al.,
bioRxiv - Pharmacology and Toxicology 2022
Quote:
... The Mpp75Aa1.1-His fractions identified by SDS-PAGE were pooled and further processed using EndoTrap® HD (Hyglos GmbH, Germany) to reduce endotoxin contents (manufacturer’s instruction) ...
-
No products found
because this supplier's products are not listed.
Alice Melocchi, et al.,
bioRxiv - Bioengineering 2023
Quote:
... with 5% AB human serum (Access Biologicals, UC-CA) and 1% L-glutamine (Thermo Fisher Scientific ...
-
No products found
because this supplier's products are not listed.
Jeanne Corriveau, et al.,
bioRxiv - Cancer Biology 2024
Quote:
... Human umbilical vein ECs (HUVECs) obtained from VEC Technologies were cultured in M199 media supplemented with 20% FBS ...
-
No products found
because this supplier's products are not listed.
Nirupa Nagaratnam, et al.,
bioRxiv - Biochemistry 2022
Quote:
... The supernatant containing detergent solubilized His-tagged FopA was filtered through a 0.22 μm cellulose acetate membrane filter (Foxx Life Sciences) and loaded at 1 mL/min onto a Ni-NTA column (HisTrap HP ...
-
No products found
because this supplier's products are not listed.
Sofia Rossini, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... while recombinant human IDO1 protein was obtained by Giotto Biotech. Construct expressing murine Src was obtained from Origene.
-
No products found
because this supplier's products are not listed.
Xiaowei Sun, et al.,
bioRxiv - Cell Biology 2024
Quote:
... Recombinant human insulin (M9194) was purchased from AbMole (Houston, USA). The ARF1 inhibitor Golgicide A (HY-100540 ...
-
No products found
because this supplier's products are not listed.
Natsuki Shichinohe, et al.,
bioRxiv - Biochemistry 2022
Quote:
Human bone ALP was purchased from CALZYME (San Luis Obispo, CA). Human CD73 His-tag was purchased from BPS Bioscience (San Diego ...
-
No products found
because this supplier's products are not listed.
Anna Ilaria Przytula-Mally, et al.,
bioRxiv - Biophysics 2023
Quote:
... for the human CPEB3 complex and PEG/Ion screen (Hampton Research) for the chimpanzee complex ...
-
No products found
because this supplier's products are not listed.
Erica Misner, Min Zhang, Eva Sapi,
bioRxiv - Microbiology 2022
Quote:
... PCR products were analyzed by standard agarose gel electrophoresis in a 1% gel referenced against a Hi-Lo DNA ladder (Bionexus, Inc., Oakland, CA, USA; BN2050). An infected zebrafish sample which was positive for B ...
-
No products found
because this supplier's products are not listed.
Camilla Kjeldgaard Larsen, et al.,
bioRxiv - Synthetic Biology 2023
Quote:
... The cleaved proteins were incubated with Ni-NTA beads for revers his-purification and cleaved proteins were collected in the flow through and visualized on SDS-PAGE (Mini Protean Tris-Tricine Precast gels, BioRad).
-
No products found
because this supplier's products are not listed.
Hongbing Zhang, et al.,
bioRxiv - Bioengineering 2020
Quote:
The E-ALPHA® human scFv antibody phage display libraries (Eureka Therapeutics) were used for the selection of human antibody constructs specific to SARS-CoV-2 spike protein ...
-
No products found
because this supplier's products are not listed.
Pirunthan Perampalam, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... Cytokeratin (human specific cytokeratin 7 and 8, ZETA Corporation #Z2018ML, 1:200), EpCAM (Cell Signaling Technologies ...
-
No products found
because this supplier's products are not listed.
Erdem D. Tabdanov, et al.,
bioRxiv - Cancer Biology 2020
Quote:
Human CD4+ T cells were plated on a glass-bottom dish (Willco Wells) pre-coated with either ICAM-1 (Life Technologies ...
-
No products found
because this supplier's products are not listed.
Guillaume N. Fiches, et al.,
bioRxiv - Microbiology 2021
Quote:
... supplemented with 10% heat-inactivated human serum (Advanced Biotechnologies Inc, Cat#P2-203), 100 U/mL penicillin ...
-
No products found
because this supplier's products are not listed.
Brian D. Adair, et al.,
bioRxiv - Cell Biology 2019
Quote:
... Chrono-luminescence reagent and human thrombin were purchased from Chrono-log (Havertown, PA).
-
No products found
because this supplier's products are not listed.
Barnabe D. Assogba, et al.,
bioRxiv - Cell Biology 2022
Quote:
... Membranes were overnight probed in 1/1000 Rabbit Anti-Human APOBEC3G (Immunodiagnostics Inc.), washed three times ...
-
No products found
because this supplier's products are not listed.
Madelon M.E. de Jong, et al.,
bioRxiv - Immunology 2023
Quote:
... slides were incubated with anti-human CD15 (1:10, C3D-1, Diagnostic BioSystems) for 1 hour at 37°C ...
-
No products found
because this supplier's products are not listed.
MU Wagenhäuser, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... two-color analysis of murine or human platelet activation was performed using fluorophore-labeled antibodies for P-selectin expression (Emfret Analytics, Wug.E9-FITC ...