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... Collagen-Antibody-Induced-Arthritis (CAIA) was induced according to manufacture instructions (MD Biosciences). Mice were maintained on a C57BL/6J genetic background.
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... membranes were stripped at 4°C in agitation using Antibody stripping buffer 1X for 10 minutes (Gene Bio-Application). Protein bands were quantified using ImageJ software ...
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... Cells were washed and stained with antibodies against cell surface molecules in PBS containing 4% of human serum (Valley Biomedical). NGFR was detected with a 20 μl per million cells of Alexa Flour 647 conjugated α-NGFR (BD Bioscience) ...
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... were labeled with biotinylated anti-His tag monoclonal antibody (ThermoScientific) and used to capture His-tagged clade C gp120 Du151 protein (Immune Technologies). The gp120-expressing beads were then incubated with triplicate 5-fold dilutions of heat-inactivated serum samples in V-bottom plates for 1h at 37°C ...
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... We washed out excess antibody with BRB80 and then incubated the flow channel with 1% Pluronic F-127 (PK-CA707-59000, PromoCell Inc., Heidelberg, Germany) for 5 min to passivate the surface ...