-
No products found
because this supplier's products are not listed.
Thomas B. Bailey, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... in the presence of protease inhibitors (Proteoloc Protease Inhibitor Cocktail, Expedeon) and then sheared in a Bioruptor sonicator for a total of 30 min (high output 30 sec on 30 sec off for 10 minutes 3× ...
-
No products found
because this supplier's products are not listed.
Mary Catherine Bridges, et al.,
bioRxiv - Cell Biology 2023
Quote:
... and 1% Triton X-100) containing 2X protease (Protease Inhibitor Cocktail III, RPI) and phosphatase inhibitors (Halt Phosphatase Inhibitor Cocktail ...
-
No products found
because this supplier's products are not listed.
Varda Lev-Ram, et al.,
bioRxiv - Neuroscience 2023
Quote:
... and heparin (20U/ml) for 2 min at 35°C followed by 0.15M cacodylate buffer containing 2.5% glutaraldehyde (Polysciences), 2% formaldehyde (Fisher Scientific ...
-
No products found
because this supplier's products are not listed.
Daniel A. Zuppo, et al.,
bioRxiv - Developmental Biology 2022
Quote:
... Fluorescein goat anti-chicken antibody was used as secondary antibody (1:200; Aves Labs, F-1005) and slides were incubated for 2 hours at RT ...
-
No products found
because this supplier's products are not listed.
Jordan Clark, et al.,
bioRxiv - Immunology 2024
Quote:
... serine 2 (TMPRSS2, BPS Biosciences, 78081) were cultured in Dulbecco’s modified Eagle medium (DMEM ...
-
No products found
because this supplier's products are not listed.
Idil Ulengin-Talkish, et al.,
bioRxiv - Biochemistry 2021
Quote:
... The phosphospecific antibody against Serine 485 site in FAM126A was manufactured by 21st Century Biochemicals as follows ...
-
No products found
because this supplier's products are not listed.
Yang Dong, et al.,
bioRxiv - Biophysics 2022
Quote:
... buffer was replaced with fresh buffer containing unlabeled L-serine and 0.1 Ci of [14C] L-serine (Moravek Biochemicals ...
-
No products found
because this supplier's products are not listed.
Longlong Si, et al.,
bioRxiv - Microbiology 2020
Quote:
... were transfected with 2·5 µg serine protease expression plasmid or empty vector using TransIT-X2 Dynamic Delivery System (Mirus). One day later ...
-
FK506-binding protein 38 (FKBP38), a member of the immunophilin family, has been implicated to...
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Konner Cool, et al.,
bioRxiv - Microbiology 2021
Quote:
... VA) and Vero E6 cells stably expressing transmembrane serine protease 2 (Vero-E6/TMPRSS2)7 were obtained from Creative Biogene (Shirley, NY) via Kyeong-Ok Chang at KSU and used for virus propagation and titration ...
-
No products found
because this supplier's products are not listed.
Wenlin Liao, Kun-Ze Lee, San-Hua Su, Yuju Luo,
bioRxiv - Neuroscience 2020
Quote:
... phosphorylated KCC2 at serine 940 (pKCC2-S940; 1:5,000, PhosphoSolutions), phosphorylated KCC2 at threonine 1007 (pKCC2-T1007 ...
-
No products found
because this supplier's products are not listed.
B. Stolp, et al.,
bioRxiv - Immunology 2019
Quote:
... Pertussis toxin (PTx) and enzymatically inactive mutant PTx (PTxmut) were obtained from List Biological Laboratories. Sodium Pyruvate (100 mM ...
-
No products found
because this supplier's products are not listed.
Kenji M. Fujihara, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... Lysates were sonicated for 35 min (S220 Focused-ultrasonicator, Covaris), followed by precipitation of cellular debris with 10% TritonX-100 ...
-
No products found
because this supplier's products are not listed.
Isabel Bennett, Alice Pyne, Rachel McKendry,
bioRxiv - Biophysics 2019
Quote:
... in a 35 mm diameter glass bottom petri dish (WillCo Wells, Netherlands). The cantilever spring constant was calibrated using the thermal noise method in the JPK software to convert vertical deflection from volts to nm ...
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No products found
because this supplier's products are not listed.
M.A.M. Vis, et al.,
bioRxiv - Bioengineering 2023
Quote:
... 28 and 35 using an ELISA (MBS162789, MyBioSource, San Diego, CA, USA) according to the manufacturer’s protocol (n=2+3 ...
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No products found
because this supplier's products are not listed.
Hana Valenta, et al.,
bioRxiv - Biophysics 2021
Quote:
... and 7.5 mM glucose) at 35°C using SynergyH1 plate reader (Biotek, USA). L-012 (100 μM ...
-
No products found
because this supplier's products are not listed.
Natasha Haskey, et al.,
bioRxiv - Immunology 2021
Quote:
... Protease inhibitor was added to sera (Protease Inhibitor Cocktail, VWR Life Science Amresco) and analyzed for metabolic hormones (Mouse Metabolic Array ...
-
No products found
because this supplier's products are not listed.
Christina Grimm, et al.,
bioRxiv - Neuroscience 2021
Quote:
... body temperature was kept at 35 °C using a heating pad (Harvard Apparatus, USA). Following implantation ...
-
No products found
because this supplier's products are not listed.
Lee Min Leong, et al.,
bioRxiv - Neuroscience 2024
Quote:
... and Ulla proteins were concentrated to 35 ∼ 50 mg/ml using a VIVASPIN20 (Sartorius) concentrator and stored at −80 °C until use.
-
No products found
because this supplier's products are not listed.
Brandon Alexander Holt, et al.,
bioRxiv - Bioengineering 2019
Quote:
... Outer Membrane Protease T (i.e., OmpT, Protease 7) was purchased from Lifespan Biosciences (Seattle, WA). OmpT fluorescent peptide substrate was custom ordered from Genscript (Piscataway ...
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No products found
because this supplier's products are not listed.
Tamara Boschert, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... were hydrophilized with 35 % EtOH and subsequently coated with anti-human IFNγ (1-D1K, Mabtech). Blocking was performed with X-Vivo-20 (Lonza ...
-
No products found
because this supplier's products are not listed.
Stamatis Papathanasiou, et al.,
bioRxiv - Cell Biology 2022
Quote:
... cells were instead plated on MembraneRing 35 rings (hereafter “membrane rings”; #415190-9142-000; Carl Zeiss) (see “Single-cell capturing” section ...
-
No products found
because this supplier's products are not listed.
Yue Liu, et al.,
bioRxiv - Microbiology 2021
Quote:
... at 80°C for 10 minutes in 10−4 final dilution of commercial stock as described earlier (35) or with GelRed nucleic acid gel stain (Biotium,10−4 final dilution of commercial stock ...
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No products found
because this supplier's products are not listed.
Zhou Zhou, et al.,
bioRxiv - Immunology 2023
Quote:
... protease inhibitors and phosphatase inhibitors (Boster Biological Tech, Wuhan, China). Immunoprecipitation was performed with anti-His or Flag magnetic beads (Bimake ...
-
No products found
because this supplier's products are not listed.
Alissa C. Greenwald, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... All antibodies (including pre-conjugated antibodies from Akoya) were additionally validated on the respective GBM control samples using multiple CODEX runs to adjust antibody concentration and exposure times to optimize signal to noise ratio ...
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No products found
because this supplier's products are not listed.
Jaclyn A. Wisinski, et al.,
bioRxiv - Physiology 2021
Quote:
... Anti-insulin antibodies (Insulin + Proinsulin Antibody, 10R-I136a; Insulin + Proinsulin Antibody, biotinylated, 61E-I136bBT) were from Fitzgerald Industries (Acton ...
-
No products found
because this supplier's products are not listed.
Chongping Li, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... and goat anti-mouse IgG secondary antibody (L3032, Signalway Antibody).
-
No products found
because this supplier's products are not listed.
Roy G. Muriu, Jessica M. Sage, Abdulbaki Agbas,
bioRxiv - Neuroscience 2019
Quote:
... MBL antibodies (MBL International, 15A Constitution way ...
-
No products found
because this supplier's products are not listed.
Subhasri Ghosh, et al.,
bioRxiv - Cell Biology 2022
Quote:
... Casper-1 antibody (AdipoGen) and corresponding mouse IgG (CST ...
-
No products found
because this supplier's products are not listed.
Mehrdad Zarei, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... The primary antibodies were diluted (SignalStain Antibody Diluent; 8112) and incubated overnight at 4 °C using the following antibodies: Ki67 (SP6, BioCare Medical) and cleaved caspase 3 (Cell Signaling Technology ...
-
No products found
because this supplier's products are not listed.
Derek D.C. Ireland, et al.,
bioRxiv - Neuroscience 2020
Quote:
... Primary antibodies used include: human polyclonal anti-Zika virus antibody (Kerafast EVU302, MA) or mouse anti-ZikV E protein mAb (Fitzgerald ...
-
No products found
because this supplier's products are not listed.
Bjoern Traenkle, et al.,
bioRxiv - Immunology 2021
Quote:
... Bound antibody was visualized using donkey-anti-rabbit-Cy3 secondary antibody (Dianova, Germany). YO-PRO-1 (Invitrogen ...
-
No products found
because this supplier's products are not listed.
Anastasia I. Kurtova, et al.,
bioRxiv - Developmental Biology 2023
Quote:
... and anti-FMRFamide antibody (Immunostar, Hudson ...
-
No products found
because this supplier's products are not listed.
Charlotte A. Dawson, et al.,
bioRxiv - Molecular Biology 2024
Quote:
Serine protease (SVSP) activity was determined using an absorbance-based Chromogenic S-2288 assay (Chromogenix, 82085239). Venoms were diluted to 0.066 mg/mL and 15 μL added per well in quadruplicate to a 384-well plate before incubation for 3 mins at 37 °C ...
-
No products found
because this supplier's products are not listed.
Reto B. Cola, et al.,
bioRxiv - Neuroscience 2024
Quote:
... and Met-Enkephalin (3000nM; Phoenix Pharmaceuticals; #024-35). Of note ...
-
No products found
because this supplier's products are not listed.
Xuetao Sun, Sara S Nunes,
bioRxiv - Pathology 2021
Quote:
... 20% from protein and 35% from carbohydrate (Research Diets, New Brunswick, NJ, USA). After 8-week HFD feeding ...
-
No products found
because this supplier's products are not listed.
J. Lützkendorf, et al.,
bioRxiv - Neuroscience 2024
Quote:
... intracellular electrodes with a resistance of 20−35 MΩ (filled with 3M KCl) were placed at muscle 6 of the abdominal segment A2/A3 ...
-
No products found
because this supplier's products are not listed.
Ka M. Pang, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... transfected cells were plated onto 35 mm Delta TPG dish (Bioptechs, Butler, PA16002, USA) for 24 h ...
-
No products found
because this supplier's products are not listed.
Agnes Moe, et al.,
bioRxiv - Biochemistry 2021
Quote:
... Cells were broken with a cell disrupter with 4 cycles at 35 kPsi (Constant Systems) and cellular debris was removed by centrifugation at 90 000 x g for 20 min at 4°C (45 Ti rotor ...
-
No products found
because this supplier's products are not listed.
Kirsten J. Meyer, Justin R. Nodwell,
bioRxiv - Microbiology 2023
Quote:
... Samples were then applied in parallel to size exclusion columns (qEV1, 35 nm IZON Sciences) and separated with PBS elution ...
-
No products found
because this supplier's products are not listed.
Clarissa Li, Adam W. Li,
bioRxiv - Pharmacology and Toxicology 2021
Quote:
... Protease cleavage (PC) assay buffer (EpiGentek) was used as the assay solutions ...
-
No products found
because this supplier's products are not listed.
Yi-Seon Jeon, et al.,
bioRxiv - Animal Behavior and Cognition 2023
Quote:
... we reconstituted protease-free chABC (Amsbio) in PBS to a final concentration of 61U/ml ...
-
No products found
because this supplier's products are not listed.
Ciro Maurizio Amato, et al.,
bioRxiv - Developmental Biology 2023
Quote:
... Culture inserts were placed inside either a 35 mm glass bottom petri dish (Ted Pella 14022-1120) or a 12 well-glass bottom culture plate (Cellvis P12-1.5H-N ...
-
No products found
because this supplier's products are not listed.
Takafumi Kato, et al.,
bioRxiv - Pathology 2021
Quote:
... SUMOstar protease (SP4110, LifeSensors; 10 units/mg substrate) was added to dialyzed protein and the reaction was incubated overnight at 4°C to cleave the His-SUMO tags ...
-
No products found
because this supplier's products are not listed.
Qingtao Sun, et al.,
bioRxiv - Neuroscience 2023
Quote:
... supplemented with Protease Inhibitor Cocktail (#AA-PI; Raybiotech) and Phosphatase Inhibitor Cocktail Set I (#AA-PHI-I ...
-
No products found
because this supplier's products are not listed.
Mario K. Shammas, et al.,
bioRxiv - Cell Biology 2021
Quote:
... primary antibody followed by secondary antibodies (Nanogold, Nanoprobes, Yaphank, NY) for 1-2 hours ...
-
No products found
because this supplier's products are not listed.
Sharon Ben-Hur, et al.,
bioRxiv - Developmental Biology 2023
Quote:
... Primary antibodies used: anti-Rubicon antibody (1:100, Biorbyt, orb186000), Anti-Rubicon/Baron antibody (1:100 ...
-
No products found
because this supplier's products are not listed.
Farès Ousalem, et al.,
bioRxiv - Microbiology 2023
Quote:
... an HRP-conjugated antibody (Anti-rabbit IgG, antibody [HRP] from COVALAB) diluted 20,000x in PBS was used ...
-
No products found
because this supplier's products are not listed.
Zhenzhen Chen, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... β-actin (catalog no. RM2001) antibody was purchased from Beijing Ray antibody Biotech. ZIC2 (catalog no. ARP35821_P050) antibody was purchased from Aviva Systems Biology ...
-
No products found
because this supplier's products are not listed.
Yuebiao Feng, et al.,
bioRxiv - Microbiology 2021
Quote:
... Immunoblotting was performed using standard procedures using the antibodies rabbit anti‐Per1 antibody (1:5000) and mouse anti‐β‐Actin antibody (1:2000) (Abbkine, China). The rabbit polyclonal anti‐Per1 antibody was generated against recombinant Per1 protein (recPer1 ...
-
No products found
because this supplier's products are not listed.
Bjoern Traenkle, et al.,
bioRxiv - Immunology 2021
Quote:
... M13-HRP-labeled antibody (Progen) was added at a conc ...