-
No products found
because this supplier's products are not listed.
Salman Shehzada, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... anti-His (Proteintech, 66005-1-Ig); anti-long dsRNA J2 (Jena Bioscience RNT-SCI-10010200) ...
-
No products found
because this supplier's products are not listed.
Anurag Adhikari, et al.,
bioRxiv - Immunology 2021
Quote:
... were cloned into pCEP4 mammalian expression vector containing N-terminal human Ig kappa leader sequence and C-terminal Avi-tag and His-tag (Invitrogen, USA). Expi293-Freestyle cells cultured at 37°C and 8% CO2 in growth medium containing Expi293 Expression Medium at 3×106/mL in 50 mL media were transfected overnight at 37 °C with 50μg of plasmid in 160μL of ExpiFectamine plus 6mL of OptiMEM-I (all from Invitrogen) ...
-
No products found
because this supplier's products are not listed.
Nitin Hingankar, et al.,
bioRxiv - Immunology 2021
Quote:
... Ig kappa or Ig lambda constant domains using the Quick Ligase cloning system (New England BioLabs) according to the manufacturer instructions ...
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No products found
because this supplier's products are not listed.
Ida Lindeman, et al.,
bioRxiv - Immunology 2023
Quote:
... 3 µg/ml recombinant human TG2 or 2 µg/ml goat anti-human Ig (Southern Biotech) was coated in PBS followed by incubation with culture supernatants diluted in PBS supplemented with 0.1% (v/v ...
-
No products found
because this supplier's products are not listed.
Filip Mihalic, et al.,
bioRxiv - Biochemistry 2022
Quote:
Human embryonic kidney 293 cells (HEK293) (Sigma), HEK293T (TakaraBio) ...
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No products found
because this supplier's products are not listed.
Yong Zhang, et al.,
bioRxiv - Biochemistry 2020
Quote:
... via baculovirus and S protein (S1 and RBD, His tag) expressed in human embryonic kidney cells (HEK293) were purchased from Sino Biological (Beijing, China). Sequencing-grade trypsin and Glu-C were obtained from Enzyme & Spectrum (Beijing ...
-
No products found
because this supplier's products are not listed.
K. Ehring, et al.,
bioRxiv - Cell Biology 2023
Quote:
... HEK293-derived human Shh (R&D Systems, 8908-SH) served as a bioactivity control and to quantify Bosc23-expressed ...
-
No products found
because this supplier's products are not listed.
Irina Buckle, et al.,
bioRxiv - Immunology 2022
Quote:
... human IL-12p70 (HEK293) (Peprotech) and 50ng/ml recombinant human IL-18 (MBL International ...
-
No products found
because this supplier's products are not listed.
Jérôme Wahis, et al.,
bioRxiv - Neuroscience 2020
Quote:
... human embryonic kidney cells 293 (HEK293; Agilent #240073) were calcium phosphate-transfected with the recombinant AAV2 plasmid and a 3-helper system ...
-
No products found
because this supplier's products are not listed.
Franziska Jäger, et al.,
bioRxiv - Biochemistry 2020
Quote:
... Cells were disrupted using a Constant cell disruptor (Constant Systems) by two or three passages at 25 000 psi or 39 000 psi when extracted from E ...
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No products found
because this supplier's products are not listed.
Nam-Kyung Lee, et al.,
bioRxiv - Bioengineering 2022
Quote:
... stained with hematoxylin and eosin (H&E) or anti-human Ig-lambda light chain antibody (Abcam) using methods described (Su et al. ...
-
No products found
because this supplier's products are not listed.
Valentin J. Heim, et al.,
bioRxiv - Cell Biology 2020
Quote:
... goat anti human Ig (109-035-003, Jackson Immunoresearch).
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No products found
because this supplier's products are not listed.
Alexander Kuzin, et al.,
bioRxiv - Genomics 2021
Quote:
... human Hek293 DNA was purchased from Genscript. S ...
-
No products found
because this supplier's products are not listed.
Pablo Hernández-Ortego, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Fura2-AM was excited at 340 and 380 nm with a Lambda 10-2 filter wheel and a Lambda LS xenon arc lamp (Sutter Instruments, USA). Emission fluorescence was filtered with a 510 nm long-pass filter ...
-
No products found
because this supplier's products are not listed.
Fynn M. Hansen, et al.,
bioRxiv - Systems Biology 2020
Quote:
HEK293 (human, DMSZ, ACC 635) and U2OS (human, American Type Culture Collection [ATCC], HTB-96) cell were cultivated in DMEM (Gibco ...
-
No products found
because this supplier's products are not listed.
Magdalena Oroń, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... Human 26S proteasome purified from HEK293 cells was purchased from Promega. The proteasome chaperoning reaction tests were carried out as follows ...
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No products found
because this supplier's products are not listed.
Aswini Panigrahi, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... from the expression vector pcDNA3.1/Myc-His(-)-HSulf-2 bearing human Sulf-2 cDNA (a gift from Steven Rosen, Addgene plasmid # 13004) (Morimoto-Tomita et al. ...
-
No products found
because this supplier's products are not listed.
Vladimir G. Magalhães, et al.,
bioRxiv - Immunology 2023
Quote:
... only donors were included that had no history of COVID-19 as well as had tested negative in three serological assays (EuroImmun-Anti-SARS-CoV-2 ELISA IgG /S1/, Siemens Healthineers SARS-CoV-2 IgG /RBD/, and Roche Elecsys Ig /Nucleocapsid Pan Ig/). PBMC were selected from heparinized full blood by a standard density gradient (Pancoll Separating Solution ...
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No products found
because this supplier's products are not listed.
Anny Gravdal, et al.,
bioRxiv - Cell Biology 2020
Quote:
Human Embryonic Kidney 293 (HEK293) cells (Clontech Laboratories) were used for most experiments described in this study ...
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No products found
because this supplier's products are not listed.
Antonia Sophia Peter, et al.,
bioRxiv - Immunology 2021
Quote:
... anti-mouse Ig light chain lambda APC # 407306 and anti-mouse IgG light chain kappa PE both from Biolegend, San Diego ...
-
No products found
because this supplier's products are not listed.
Nam-Kyung Lee, et al.,
bioRxiv - Bioengineering 2022
Quote:
... and free human Ig-lambda light chains in serum was assessed using a Human Lambda ELISA Kit (Bethyl Laboratories) according to manufacturer’s instructions ...
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No products found
because this supplier's products are not listed.
Subeena Sood, et al.,
bioRxiv - Immunology 2023
Quote:
HEK293 cells expressing human ACE2 and TMPRSS2a (Invivogen) were seeded at 20,000 cells/well in a gelatin-coated 96-well plate (Corning ...
-
No products found
because this supplier's products are not listed.
Rozemarijn E. van der Veen, et al.,
bioRxiv - Cell Biology 2023
Quote:
... equipped with a Plan APO Lambda 100x/1.45 NA lambda oil objective (Nikon) and controlled by NIS Elements (Nikon) ...
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No products found
because this supplier's products are not listed.
Yadira M. Soto-Feliciano, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... Human HEK293 cells were maintained in DMEM (Corning) supplemented with 10% FBS (Gibco) ...
-
No products found
because this supplier's products are not listed.
Ivan K. Domingo, et al.,
bioRxiv - Pharmacology and Toxicology 2022
Quote:
... HEK293-Null2 cells were also co-transfected with a human MD-2 expression clone (OriGene, RC204686). JetPRIME (Polyplus ...
-
No products found
because this supplier's products are not listed.
Francina Mesquida-Veny, et al.,
bioRxiv - Cell Biology 2022
Quote:
... rat immunoglobulin (Ig) G (Merck, 2-5μg/ml), U0126 (Promega ...
-
No products found
because this supplier's products are not listed.
Yoon-Hee Jung, et al.,
bioRxiv - Genomics 2022
Quote:
Adult (2-5 months old) CD-1 IGS mice (Charles River Lab, USA) were used for all experiments ...
-
No products found
because this supplier's products are not listed.
Sophia Lambert, Jakub Voznica, Hélène Morlon,
bioRxiv - Evolutionary Biology 2022
Quote:
... time constant birth-death (BD) model ...
-
No products found
because this supplier's products are not listed.
John T. Killian Jr., et al.,
bioRxiv - Immunology 2023
Quote:
... and DNA amplicon products where generated using Ig-specific variable and constant gene primers.87 Samples were indexed (Nextera Index Kit, Illumina), purified ...
-
No products found
because this supplier's products are not listed.
Man Zhou,
bioRxiv - Biophysics 2021
Quote:
... 2 g/ml Biotinylated Anti-His Antibody (Penta-His Biotin Conjugate; Qiagen; No. 34440) in T50 buffer was introduced into flow cells at 50 μl/min ...
-
No products found
because this supplier's products are not listed.
Tzu-Hsuan Chang, et al.,
bioRxiv - Immunology 2023
Quote:
Recombinant 50 kDa and 39 kDa CHIT1 proteins were expressed as C-terminally 8xHis-tagged constructs in HEK293-6E cells and purified from the cell culture supernatant by His-Trap (GE Healthcare) affinity as described in [42] ...
-
No products found
because this supplier's products are not listed.
Sumit Sen Santara, et al.,
bioRxiv - Immunology 2021
Quote:
... NKp30-Ig (Acrobiosystems), hemagglutinin His-tag (SinoBiological) ...
-
No products found
because this supplier's products are not listed.
Anna I Wurz, Kevin Zheng, Robert M. Hughes,
bioRxiv - Synthetic Biology 2024
Quote:
... total ERK using p44/42 MAPK (ERK1/2) rabbit Ig (Cell Signaling Technologies). Blots were washed with TBST 3x for 5 min and incubated in corresponding secondary antibody for 1 hour room temperature ...
-
No products found
because this supplier's products are not listed.
Laura Alonso-Herranz, et al.,
bioRxiv - Immunology 2020
Quote:
HEK293 cells (Lonza) were cultured in DMEM (Lonza ...
-
No products found
because this supplier's products are not listed.
Jillian M. DiMuzio, et al.,
bioRxiv - Immunology 2021
Quote:
... HEK293 cells expressing human Angiotensin converting enzyme 2 (ACE2) (BPS Biosciences, Cat #79951) were cultured in EMEM containing 10% FBS and 5 mg/mL Puromycin to select for ACE2-expressing cells ...
-
No products found
because this supplier's products are not listed.
Wenjuan Yang, et al.,
bioRxiv - Immunology 2021
Quote:
... human recombinant IL-2 (Miltenyi Biotec) was added to the medium every two days from day 2 on (100 U/ml) ...
-
No products found
because this supplier's products are not listed.
Carolin Magdalene Heise, et al.,
bioRxiv - Plant Biology 2023
Quote:
... and 750 nm using a spectrophotometer (Perkin Elmer UV/VIS spectrometer Lambda 2). The pigment contents were calculated according to the equation given by Wellburn ...
-
No products found
because this supplier's products are not listed.
Kerrie L. Marie, et al.,
bioRxiv - Cancer Biology 2019
Quote:
... Anti-Rabbit Ig (Vector Laboratories). Chromagen staining was done using ImmPACT™ Vector Red Alkaline Phophatase substrate kit (Vector Laboratories) ...
-
No products found
because this supplier's products are not listed.
Kusal T. G. Samarasinghe, et al.,
bioRxiv - Cancer Biology 2021
Quote:
Click reaction mixtures and unreacted alkyne-modified oligonucleotides were separated in a 1.2 % agarose gel for 1 h at constant 120 mV and DNA bands were captured by the ChemiDoc system (BioRad) using SYBR safe mode.
-
No products found
because this supplier's products are not listed.
Hoang Nguyen, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... and human recombinant IL-2 (Stemcell Technologies, Vancouver, Canada). Activated CD4+ T cells were transfected with pVax-CasRx and gRNA plasmids by nucleofection using Lonza 4d nucleofector ...
-
No products found
because this supplier's products are not listed.
Stefania Capone, et al.,
bioRxiv - Immunology 2020
Quote:
... recombinant human His-tagged ACE-2 protein (RayBiotech) was incubated on cells before adding any antibodies ...
-
No products found
because this supplier's products are not listed.
Liliana R. Teixeira, et al.,
bioRxiv - Biophysics 2023
Quote:
Lambda phosphatase was purchased from Santa Cruz Biotechnologies ...
-
No products found
because this supplier's products are not listed.
Masaya Yamaguchi, et al.,
bioRxiv - Microbiology 2019
Quote:
Human TLR2/NF-κB/SEAP stably transfected HEK293 cells and human TLR4/MD-2/CD14/NF-κB/SEAP stably transfected HEK293 cells (Novus Biologicals, Centennial ...
-
No products found
because this supplier's products are not listed.
Mengjia Lu, et al.,
bioRxiv - Microbiology 2023
Quote:
... Cells were then transfected with ACE2-Ig-95 or ACE2-Ig-105/106 plasmid using PEI 25K (Polysciences, Cat. No. 23966-1) and selected using methionine sulfoximine (MSX)-containing EX-CELL CD CHO Fusion Medium (Sigma ...
-
No products found
because this supplier's products are not listed.
Laura M. Canaday, et al.,
bioRxiv - Microbiology 2021
Quote:
... and IL-8) (Meso Scale Discovery) and the DIY Human IFN Lambda 1/2/3 (IL-29/28A/28B) ELISA (PBL Assay Science) according to the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Jianan Zhang, et al.,
bioRxiv - Biophysics 2024
Quote:
... His-tagged dROS1 protein (construct 2-3) were loaded onto penta-His sensors (Sartorius) to a response unit of about 0.5 nm followed by quenching in 10 ng/mL biocytin (Sigma-Aldrich ...
-
No products found
because this supplier's products are not listed.
Jonathan S Schor, et al.,
bioRxiv - Neuroscience 2021
Quote:
An isolated constant current bipolar stimulator (WPI) was used to deliver electrical stimuli ...
-
No products found
because this supplier's products are not listed.
Mami Yasukawa, et al.,
bioRxiv - Cancer Biology 2019
Quote:
... Anti-hTERT mouse mAb (clone 2E4-2) and Mouse TrueBlot ULTRA Anti-Mouse Ig HRP (Rockland) or anti-hTERT rabbit mAb (ab32020 ...
-
No products found
because this supplier's products are not listed.
Tomás Dias, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... under constant rotation in LoBind microcentrifuge tubes (Eppendorf). The amount of capture antibody used in the reactions was 10 µg per mg of MBs ...
-
No products found
because this supplier's products are not listed.
Ruby Schipper, et al.,
bioRxiv - Cell Biology 2024
Quote:
... P/S and 2% human serum (VWR/BioWest, cat. #S4190-100). The culture insert’s apical site was supplemented with 300uL and the basolateral site with 800uL media ...