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Citations for Bio-Rad :
1 - 50 of 1295 citations for Ig lambda constant 2 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: Click reaction mixtures and unreacted alkyne-modified oligonucleotides were separated in a 1.2 % agarose gel for 1 h at constant 120 mV and DNA bands were captured by the ChemiDoc system (BioRad) using SYBR safe mode.
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bioRxiv - Microbiology 2023Quote: ... Goat F(ab)2 anti-human IgG: HRP (Biorad), Goat anti-mouse IgG (H/L) ...
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bioRxiv - Molecular Biology 2022Quote: ... goat anti-human IgG F(ab’)2 (STAR126, Bio-Rad), anti-M13-pVIII-HRP (Cytiva 27-9421-01 ...
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bioRxiv - Biochemistry 2021Quote: ... Goat anti-rabbit (170-6515)) Ig secondary antibody was purchased from BioRad.
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bioRxiv - Cell Biology 2021Quote: ... and a constant current of 10 mA per gel (Bio-Rad Mini-PROTEAN) was applied ...
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bioRxiv - Biochemistry 2020Quote: ... with goat anti-human IgG F(ab’)2-HRP (STAR126P, Bio-Rad) as the detection antibody.
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bioRxiv - Microbiology 2022Quote: ... mouse-anti His (BIO-RAD; MCA1396GA), mouse-anti p65 (F-6 ...
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bioRxiv - Microbiology 2023Quote: ... A mouse anti-His antibody (Biorad) was used as a primary antibody in a 1:500 dilution ...
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bioRxiv - Immunology 2021Quote: ... sections were stained with 0.4 µg/mL rabbit -anti-human CD20 polyclonal antibodies (Neomarkers) and 2 µg/mL rat-anti-human CD3 antibodies (clone MCA1477, BioRad). Then ...
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bioRxiv - Microbiology 2023Quote: ... The was filtered through a 0.45 μm filter and His-MSI-2 was purified with HisTrapTM FF column (Cytiva) using the Econo Gradient Pump (BIORAD) according to the manufacturer’s protocol and overnight dialysis ...
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bioRxiv - Biochemistry 2020Quote: ... Detection antibodies used: anti-human F(ab’)2 IgG-HRP (STAR126P, Bio-Rad), anti-His5-Tag IgG-HRP (MCA5995P ...
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bioRxiv - Biochemistry 2020Quote: ... Detection antibodies used: anti-human F(ab’)2 IgG-HRP (STAR126P, Bio-Rad), anti-His5-Tag IgG-HRP (MCA5995P ...
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bioRxiv - Microbiology 2019Quote: ... The cells were pulsed once at 1.8 kV and 2.5 msec time constant (MicroPulser, Biorad), outgrown in 1 ml of TSB containing 500 mM sucrose for 90 min at 37°C ...
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bioRxiv - Microbiology 2019Quote: ... 120° included angle and 14°C constant temperature in a CHEF-MAPPER system (Bio-Rad) with pulsed times ramped from 0.2s to 120s.
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bioRxiv - Microbiology 2022Quote: ... 120° included angle and 14 °C constant temperature in a CHEF-MAPPER system (Bio-Rad) with pulsed times ranging from 0.5 s to 3 min 10 s with a linear ramping factor.
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bioRxiv - Biochemistry 2020Quote: ... His-tagged protein was detected using a mouse anti-6×His IgG conjugated to horseradish peroxidase (MCA1396P; Bio-Rad), and the blot was developed using Pierce™ ECL Western Blotting Substrate (Thermo Fisher Scientific).
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bioRxiv - Biochemistry 2020Quote: ... Gels were run at a constant 65 W using Sequi-Gen GT Systems from Bio-Rad or vertical electrophoresis systems from CBS Scientific and visualized using an Amersham™ Typhoon™ Biomolecular Imager (GE Healthcare) ...
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bioRxiv - Biochemistry 2023Quote: ... by electro-transfer (30 V constant overnight) in the Mini Trans-Blot cell module (Bio-Rad). The membrane was blocked for 1 h at 4 °C with 3% (w/v ...
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bioRxiv - Molecular Biology 2023Quote: ... for 7 min with a constant 2.5 A using a Trans-Blot Turbo transfer system (Bio-Rad).
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bioRxiv - Biochemistry 2019Quote: ... Goat anti-rabbit (170-6515) and anti-mouse (626520) Ig secondary Abs were purchased from BioRad and Life Technologies respectively ...
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bioRxiv - Plant Biology 2020Quote: ... 12.5 μL of SensiMix SYBR Hi-ROX (BIO-RAD) and DNAse/RNAse free water up to 15 μL ...
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bioRxiv - Microbiology 2023Quote: ... and an anti-His-HRP conjugate antibody (Bio-Rad) was used in a 1:4000 dilution ...
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bioRxiv - Microbiology 2023Quote: ... or His-tag antibody (MCA1396, Bio-Rad, 1:1000), or rabbit monoclonal β-actin antibody (D6A8 ...
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bioRxiv - Cancer Biology 2022Quote: ... gel in 0.5x TBE buffer and run with Lambda Ladder (ProMegaMarkers) using CHEF-MAPPER system (BIO-RAD).
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bioRxiv - Biochemistry 2022Quote: ... Reaction samples were run at constant 150 V on Precast Mini-Protean 4-15% gradient TGX gels (BioRad).
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bioRxiv - Neuroscience 2023Quote: ... Electrophoresis was performed at a constant current of 150 mV in a Mini-PROTEAN Tetra cell (BIO-RAD). Proteins were then transferred onto nitrocellulose membrane (LI-COR Biosciences ...
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bioRxiv - Biophysics 2020Quote: The transient transfection of HEK293 cells was performed67 using the TransFectin Lipid Reagent (Bio-Rad) (New England Biolabs) ...
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bioRxiv - Biophysics 2020Quote: The transient transfection of HEK293 cells was performed67 using the TransFectin Lipid Reagent (Bio-Rad) (New England Biolabs).
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bioRxiv - Microbiology 2022Quote: ... mouse monoclonal His tag antibody (MCA1396GA, Bio-Rad, 1:1000), mouse 2A antibody (NBP2-59627 ...
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bioRxiv - Immunology 2020Quote: ... 1D SDS-PAGE were carried out on 12% gel at constant 20 mA in a Miniprotean 2D-chamber (BioRad). Supernatants obtained after depletion were mixed in a ratio of 1 ...
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bioRxiv - Systems Biology 2020Quote: ... A total of 800 µl of cell suspension mixed with 10 µg of pDM304_Red-FL2 in a 4-mm-width cuvette was subjected to electroporation (two 5 sec separated pulses with 1.0 kV and a 1.0 msec time constant) using a MicroPulser (Bio-Rad). These cells were plated on 4–6 pieces of 90-mm plastic dishes with HL5 medium and incubated at 22°C for 18 hours under the non-selective conditions and then cultured in the presence of 10 µg/ml G418 (Wako) ...
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bioRxiv - Molecular Biology 2022Quote: ... The transfer of RNA from denaturing urea gel onto nylon membrane was performed at 1.0 A constant for 15’ using a Transblot Turbo apparatus (BioRad). EDC (Sigma E7750 ...
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bioRxiv - Microbiology 2023Quote: ... 30 μg DNA total) by electroporation with a time constant between 0.16 and 0.20 msec (BioRad Gene Pulser II). After 24 h ...
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bioRxiv - Biophysics 2021Quote: ... NRG1-Ig was purified under denaturing conditions via immobilized metal affinity chromatography (IMAC) using NGC system (Bio-Rad) equipped with a 10 mL Co-NTA column ...
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bioRxiv - Cancer Biology 2022Quote: ... HEK293 cells were transfected by electroporation in OPTI-MEM I using Gene-Pulser II (Bio-Rad) with 960 μF und 0.18 kV with 10 μg pcDNA3-FLAG-JUNB ...
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bioRxiv - Biochemistry 2021Quote: ... and electroporated (2500 V, 200 Ω, 25 μF, exponential decay, time constant < 4 ms) using Gene Pulser Xcell (Bio-Rad). 1 mL BHI medium + 0.5 mM saccharose was added and the cells were incubated at 30 °C for 4 h and plated on BHI agar plates containing 10 μg/mL erythromycin ...
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bioRxiv - Cell Biology 2021Quote: ... Gels were Western blotted overnight with constant current of 200 mA using a Trans-Blot® Cell (Bio-Rad, USA). Membranes were destained from residual Coomassie traces by washes in methanol for several minutes ...
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bioRxiv - Molecular Biology 2022Quote: ... was performed the next day by using a constant amperage of 50 μA per strip in the Protean IEF Cell equipment (BioRad). Next ...
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bioRxiv - Biochemistry 2021Quote: ... and electroporated (2500 V, 200 Ω, 25 μF, exponential decay, time constant ~ 4 ms) using Gene Pulser Xcell (Bio-Rad). 1 mL warm BHI medium was added and the cells were incubated at 30 °C for 6 h under shaking at 200 rpm and plated on BHI agar plates with 10 μg/mL erythromycin ...
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bioRxiv - Immunology 2022Quote: ... and electroporated at 1500 V with an average time constant of ∼4.5 ms using a Gene Pulser Xcell Electroporation System (Bio-Rad), which was repeated for the entire transformation mix ...
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bioRxiv - Developmental Biology 2024Quote: ... at room temperature and constant voltage (100 V) in 1X MOPS Running buffer and transferred to a nitrocellulose membrane (0.45 mM, Bio-Rad) using iBlot2 system (Invitrogen) ...
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bioRxiv - Bioengineering 2024Quote: ... The gels were stained under constant shaking at 80 rpm on an Orbital Shaker D0S-10L (NeoLab) using 50 mL Coomassie stain (BioRad) overnight and imaged using a c600 gel imager (Azure Biosystems).
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bioRxiv - Microbiology 2023Quote: ... followed by incubation with mouse anti-His (1:1000, BioRad MCA1396GA) overnight at 4 °C and secondary antibody detection with IRDye800CW anti-mouse (1:5000 ...
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bioRxiv - Microbiology 2024Quote: ... PorH-His immunoblots were performed on 16.5% tris-tricine gels (BioRad) using anti-His (mouse ...
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bioRxiv - Cell Biology 2020Quote: ... The samples were then subjected to SDS-PAGE at a constant voltage of 100 V for 1 hour at room temperature and transferred to nitrocellulose membrane (BIO-RAD) at a constant voltage of 30 V at 4°C overnight ...
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bioRxiv - Pathology 2019Quote: ... 20% methanol and MilliQ water) overnight at 4°C in a wet transfer apparatus at 70 mA constant voltage (Bio-Rad). Detection of the chemiluminescence immunochemical reaction was performed on a ChemiDoc ™ XRS + Molecular Imager® (Bio-Rad ...
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bioRxiv - Molecular Biology 2019Quote: ... 5-10 pg of pUC18 plasmid were electroporated at 18,000V/cm at a time constant of 7.5 msec using Gene Pulser Xcell (Bio-Rad, Hercules, CA) into 10 μl competent cells ...
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bioRxiv - Microbiology 2022Quote: ... 20% (v/v) methanol) at a constant amperage of 300 mA for 90 min using a Mini Trans-Blot Cell (Bio-Rad). The membranes were incubated in NRA blocking buffer (INNOVAX ...
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bioRxiv - Bioengineering 2022Quote: ... run at 120 V constant voltage (Pharmacia LKB, ECPS 3000/150) using a Hoefer gel system and Precision Plus Protein Kaleidoscope standards (Bio-Rad). Protein and peptide concentrations were determined by direct measurement on a Nanodrop 1000 UV-Vis spectrophotometer (Thermo Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... or wet transferred using 0.5 M sodium phosphate transfer buffer (at a constant 1 Amp for an hour using a Bio-Rad apparatus). Membranes were blocked in 5% milk in PBS-T and probed with primary antibody (overnight at 4°C ...