-
No products found
because this supplier's products are not listed.
N Ogino, et al.,
bioRxiv - Cell Biology 2022
Quote:
... or high mobility group box-1 (HMGB1, R&D systems, 1690-HMB) at the indicated concentrations for 20 hours ...
-
No products found
because this supplier's products are not listed.
Shoib S. Siddiqui, et al.,
bioRxiv - Immunology 2020
Quote:
... Recombinant His-HMGB1 was produced in 293-freestyle cells (ThermoFisher Scientific). Purification of His-HMGB1 from 293-freestyle cell lysate was carried out using Ni SepharoseTM 6 Fast Flow gel (GE Healthcare) ...
-
No products found
because this supplier's products are not listed.
Sabira Mohammed, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... mouse HMGB1(high mobility group protein B1) ELISA Kit from Elabscience (Houston, TX). The optical density readings obtained were used to generate a four-parameter logistic curve and the concentration of the analytes in the samples were calculated by comparing to the standard curve generated.
-
No products found
because this supplier's products are not listed.
Richard M Lucas, et al.,
bioRxiv - Neuroscience 2019
Quote:
... and His-tagged human HDAC6 protein (EMD Millipore) were incubated with 57 nm 32P-ATP in kinase buffer (50 mM Tris HCl ...
-
No products found
because this supplier's products are not listed.
A-M C Overstreet, et al.,
bioRxiv - Microbiology 2023
Quote:
... 0.85 mg/mL of human HMGB1 peptide (Abcam), or 9 ug/mL of full-length FimH protein (antigen for custom antibody production ...
-
No products found
because this supplier's products are not listed.
Yuya Hirai, Keizo Tomonaga, Masayuki Horie,
bioRxiv - Microbiology 2021
Quote:
... anti-high mobility group box 1 (HMGB1) antibody (sc-74085; Santa Cruz Biotechnology, Inc., Dallas, TX, USA), goat anti-mouse immunoglobulin G (IgG ...
-
No products found
because this supplier's products are not listed.
Yong Zhang, et al.,
bioRxiv - Biochemistry 2020
Quote:
... via baculovirus and S protein (S1 and RBD, His tag) expressed in human embryonic kidney cells (HEK293) were purchased from Sino Biological (Beijing, China). Sequencing-grade trypsin and Glu-C were obtained from Enzyme & Spectrum (Beijing ...
-
No products found
because this supplier's products are not listed.
Maciej Kliszczak, et al.,
bioRxiv - Cell Biology 2023
Quote:
... rabbit anti-human Lamin B1 (12987-1-AP, Proteintech Group) at 1:1000 (IB and IF) ...
-
No products found
because this supplier's products are not listed.
Leigh A. Kotze, et al.,
bioRxiv - Immunology 2021
Quote:
... was assessed using Mouse Anti-Human HMGB1 (BioLegend, catalogue number: 651408) at a concentration of 20µg/mL ...
-
No products found
because this supplier's products are not listed.
Shoib S. Siddiqui, et al.,
bioRxiv - Immunology 2020
Quote:
... Purification of His-HMGB1 from 293-freestyle cell lysate was carried out using Ni SepharoseTM 6 Fast Flow gel (GE Healthcare). After purification ...
-
No products found
because this supplier's products are not listed.
Shengyun Ma, et al.,
bioRxiv - Immunology 2022
Quote:
... RT-PCR was performed on cDNAs from murine Th17 or human HEK293 cells using the Q5 Hot Start High-Fidelity 2X Master Mix (New England Biolabs) with the standard thermocycling protocol ...
-
No products found
because this supplier's products are not listed.
Stephanie Marsin, et al.,
bioRxiv - Biochemistry 2023
Quote:
... His tagged proteins were labeled using Ni-NTA-ATTO 488 (Merck Group) at a ratio of ATTO/protein = 1/500 in MN buffer (50 mM MES pH 6 ...
-
No products found
because this supplier's products are not listed.
Pavel I. Deryabin, et al.,
bioRxiv - Cell Biology 2021
Quote:
... HMGB1 (clone D3E5) (#6893, Cell Signaling, USA), phospho-p53 (Ser15 ...
-
No products found
because this supplier's products are not listed.
Dominique S. Stolle, et al.,
bioRxiv - Plant Biology 2022
Quote:
... bound His-mSTIC2 protein was detected by anti His HRP conjugate (Qiagen) and ECL reaction (Pierce).
-
No products found
because this supplier's products are not listed.
Ramy Malty, et al.,
bioRxiv - Immunology 2023
Quote:
... Human HMGB1/HMG-1 ELISA (Novus Biologicals, NBP2-62766) was performed as described in manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Irina Buckle, et al.,
bioRxiv - Immunology 2022
Quote:
... human IL-12p70 (HEK293) (Peprotech) and 50ng/ml recombinant human IL-18 (MBL International ...
-
No products found
because this supplier's products are not listed.
Jérôme Wahis, et al.,
bioRxiv - Neuroscience 2020
Quote:
... human embryonic kidney cells 293 (HEK293; Agilent #240073) were calcium phosphate-transfected with the recombinant AAV2 plasmid and a 3-helper system ...
-
No products found
because this supplier's products are not listed.
Alba Llibre, et al.,
bioRxiv - Immunology 2020
Quote:
... using the following: anti-human CD120a (Clone MABTNFR1-B1, BD Bioscience) antibody ...
-
No products found
because this supplier's products are not listed.
Nikolaos Kakogiannos, et al.,
bioRxiv - Cell Biology 2022
Quote:
... The molecular masses of the proteins were estimated relative to the electrophoretic mobility of a co-transferred prestained protein marker (Precision Plus Protein Standards Dual Colour; 161-0374; BioRad). Densitometry analysis of the bands was carried out used the Fiji software (open source ...
-
No products found
because this supplier's products are not listed.
Alexander Kuzin, et al.,
bioRxiv - Genomics 2021
Quote:
... human Hek293 DNA was purchased from Genscript. S ...
-
No products found
because this supplier's products are not listed.
Yadira M. Soto-Feliciano, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... Human HEK293 cells were maintained in DMEM (Corning) supplemented with 10% FBS (Gibco) ...
-
No products found
because this supplier's products are not listed.
Xiuliang Huang, et al.,
bioRxiv - Microbiology 2019
Quote:
Cell-free translated His-VqmA was produced using the S30 T7 High-Yield Protein Expression System (Promega) according to the manufacturer’s protocol with the following amendments ...
-
No products found
because this supplier's products are not listed.
Jin Wei, et al.,
bioRxiv - Genetics 2020
Quote:
HMGB1 sgRNAs were cloned into lentiCRISPRv2 (Addgene 52961) which also encodes the Cas9 gene (79) ...
-
No products found
because this supplier's products are not listed.
Alisa Potter, et al.,
bioRxiv - Molecular Biology 2023
Quote:
Human embryonic kidney 293 (HEK293) cells (ATCC; CRL-1573) were grown in high-glucose DMEM (Lonza; BE12-604F) supplemented with 10% foetal bovine serum (FBS ...
-
No products found
because this supplier's products are not listed.
Anny Gravdal, et al.,
bioRxiv - Cell Biology 2020
Quote:
Human Embryonic Kidney 293 (HEK293) cells (Clontech Laboratories) were used for most experiments described in this study ...
-
No products found
because this supplier's products are not listed.
Fynn M. Hansen, et al.,
bioRxiv - Systems Biology 2020
Quote:
HEK293 (human, DMSZ, ACC 635) and U2OS (human, American Type Culture Collection [ATCC], HTB-96) cell were cultivated in DMEM (Gibco ...
-
No products found
because this supplier's products are not listed.
Adel Avetisyan, et al.,
bioRxiv - Developmental Biology 2021
Quote:
... His-tagged proteins in soluble fraction were purified using cOmplete His-Tag Purification Columns (Roche). The columns were washed with 10 column volumes of wash buffer 1 (20 mM Tris ...
-
No products found
because this supplier's products are not listed.
Connor R. Quinn, et al.,
bioRxiv - Biochemistry 2021
Quote:
... the NASH group started receiving high fat/high sucrose diet (Research Diets # D12331i) and at 28 weeks of age the diet was switched to high fat/high fructose/high cholesterol diet (Research Diets # D09100310i) ...
-
No products found
because this supplier's products are not listed.
Jacopo Sgrignani, et al.,
bioRxiv - Biochemistry 2020
Quote:
... histidine tagged HMGB1 was labeled by the his-tag specific NT-647 dye (Monolith NTTM Protein Labelling Kit RED-NHS, NanoTemper® Technologies GmbH, Mu□nchen, Germany), for 30 minutes at room temperature ...
-
No products found
because this supplier's products are not listed.
Subeena Sood, et al.,
bioRxiv - Immunology 2023
Quote:
HEK293 cells expressing human ACE2 and TMPRSS2a (Invivogen) were seeded at 20,000 cells/well in a gelatin-coated 96-well plate (Corning ...
-
No products found
because this supplier's products are not listed.
Jean Personnaz, et al.,
bioRxiv - Pathology 2021
Quote:
... HMGB1 plasmid was purchased from Origene. 24h after transfection ...
-
No products found
because this supplier's products are not listed.
Shraddha Pai, et al.,
bioRxiv - Genomics 2021
Quote:
We downloaded publicly-available Hi-C data from human prefrontal cortex tissue23,24 (Illumina HiSeq 2000 paired-end raw sequence reads ...
-
No products found
because this supplier's products are not listed.
Kevin R. Bewley, et al.,
bioRxiv - Pathology 2020
Quote:
... high protein binding ELISA plates (PerkinElmer) were coated overnight at 4°C with rabbit anti-human IgG (Jackson Laboratories ...
-
No products found
because this supplier's products are not listed.
Sieglinde De Cae, et al.,
bioRxiv - Microbiology 2023
Quote:
... His-tagged recombinant human FcRn (FCGRT-B2M heterodimer) protein was purchased from ACROBiosystems. Bebtelovimab biosimilar and a human IgG1 isotype control antibody were used as controls in the assay ...
-
No products found
because this supplier's products are not listed.
Simone C. Kleinendorst, et al.,
bioRxiv - Cancer Biology 2024
Quote:
Serum HMGB1 levels were determined using the HMGB1 express ELISA kit according to manufacturer’s instructions (Tecan, 30164033).
-
No products found
because this supplier's products are not listed.
Maria Esméria Corezola do Amaral, et al.,
bioRxiv - Physiology 2020
Quote:
... The second group was fed for 12 weeks with high-fat-diet (ENVIGO TD.03584) containing 35.2% lipids (approximate fatty acid profile ...
-
No products found
because this supplier's products are not listed.
Jianan Zhang, et al.,
bioRxiv - Biophysics 2024
Quote:
... His-tagged dROS1 protein (construct 2-3) were loaded onto penta-His sensors (Sartorius) to a response unit of about 0.5 nm followed by quenching in 10 ng/mL biocytin (Sigma-Aldrich ...
-
No products found
because this supplier's products are not listed.
Stefania Capone, et al.,
bioRxiv - Immunology 2020
Quote:
... recombinant human His-tagged ACE-2 protein (RayBiotech) was incubated on cells before adding any antibodies ...
-
No products found
because this supplier's products are not listed.
Autumn G. York, et al.,
bioRxiv - Immunology 2023
Quote:
... Differential Mobility Device on Lipidyzer was tuned with SelexION tuning kit (Sciex 5040141). Instrument settings ...
-
No products found
because this supplier's products are not listed.
Sara Cavallaro, et al.,
bioRxiv - Bioengineering 2021
Quote:
... The general surface marker composition for the sEVs from the three cell sources (HEK293, cbMSC, THP1) was assessed by a multiplex bead-based flow cytometry assay (MACSPlex Exosome Kit, human, Miltenyi Biotec) by using an optimized workflow as described previously.[27] In brief ...
-
No products found
because this supplier's products are not listed.
Larisa Dubrovsky, et al.,
bioRxiv - Immunology 2022
Quote:
Two groups (6 male mice per group) of C57BL/6J mice (Jackson Laboratories) were administered either exNef (2 μg total protein ...
-
No products found
because this supplier's products are not listed.
Braulio Cardenas-Benitez, et al.,
bioRxiv - Biophysics 2023
Quote:
... and the media further supplemented with 30 U mL-1 of human recombinant IL-2 protein (STEMCELL Technologies, USA). T cells were incubated in a humidified incubator with 5% CO2 at 37°C and were split to 0.5×106 cells mL-1 when their concentration surpassed 2.5×106 cells per well or when medium became depleted (turning yellow in color) ...
-
No products found
because this supplier's products are not listed.
Jamin Liu, et al.,
bioRxiv - Microbiology 2022
Quote:
... Bafilomycin B1 (Cayman Chemical Company), and Concanamycin A (Santa Cruz Biotechnology ...
-
No products found
because this supplier's products are not listed.
Isha Ranadive, et al.,
bioRxiv - Developmental Biology 2021
Quote:
Morphology of the caudal fin (n=6 for each group) was observed at 60 hpa for all the three groups using a Leica DM2500 light microscope (Leica, Germany). The regenerative outgrowth was measured under the Leica DM2500 microscope fitted with an inbuilt ocular micrometer.
-
No products found
because this supplier's products are not listed.
Christin Herrmann, et al.,
bioRxiv - Microbiology 2020
Quote:
... with 0.15% v/v TFA and separated into either 3 high-pH fractions (enriched ubiquitinated peptides) or 7 high-pH fractions (global proteome) over C18 columns (The Nest Group, MicroSpin column C18 silica ...
-
No products found
because this supplier's products are not listed.
Nur F. Isa, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... His-tagged recombinant proteins were retained on nickel magnetic beads (VWR International) and GST-tagged recombinant proteins were retained on glutathione magnetic beads (Fisher Scientific) ...
-
No products found
because this supplier's products are not listed.
Sarah A. Shelby, Thomas R. Shaw, Sarah L. Veatch,
bioRxiv - Biophysics 2022
Quote:
... VCAM-1/Fc chimeric proteins and anti-human Fc antibodies (recombinant human VCAM-1/Fc chimera protein, Fisher 862-VC-100; Fcγ-specific goat anti-human IgG, Jackson Immunoresearch 109-005-008) or poly-L-lysine (Sigma P4707).
-
No products found
because this supplier's products are not listed.
Gautier Langin, et al.,
bioRxiv - Plant Biology 2023
Quote:
... For recombinant His-UBA1 and His-UBC8 protein were purified using Ni-Ted resin (Macherey-Nagel).
-
No products found
because this supplier's products are not listed.
Annaïck Carles, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... The binding reaction was performed using Odyssey Infrared electro-mobility shift assay kit (LI-COR Biosciences) according to the manufacturer protocol ...
-
No products found
because this supplier's products are not listed.
Sam C. Nalle, et al.,
bioRxiv - Immunology 2020
Quote:
HEK293 cells stably expressing fluorescently-tagged AQP3 (an AQP3-DsRed2 fusion protein) were incubated with 1 μm latex beads (Polysciences) for 2 hours prior to fixation with 2% paraformaldehyde (PFA)/0.2% glutaraldehyde ...