-
No products found
because this supplier's products are not listed.
Johanna S. Jackson, et al.,
bioRxiv - Neuroscience 2020
Quote:
... an adeno-associated virus (AAV) serotype 2 expressing GFP (1010 GU; Vector Biolabs) was injected into layer 2/3 (300µm below the dura ...
-
No products found
because this supplier's products are not listed.
Stephanie Pei Tung Yiu, et al.,
bioRxiv - Microbiology 2024
Quote:
... Precleared lysate was then incubated with 2 μg/ml anti-GFP antibody (Proteintech #50430-2-AP) for 1 hr at 4°C with rotation ...
-
No products found
because this supplier's products are not listed.
Harold Marcotte, et al.,
bioRxiv - Immunology 2021
Quote:
... The SARS-CoV-2 S1 scanning pool contains 166 peptides from the human SARS-CoV-2 virus (#3629-1, Mabtech AB). The peptides are 15-mers overlapping with 11 amino acids ...
-
No products found
because this supplier's products are not listed.
Julia L. McKechnie, et al.,
bioRxiv - Immunology 2019
Quote:
... flavivirus group antigen (4G2, Novus Biologicals) conjugated to Alexa Fluor™ 647 using Alexa Fluor 647 Antibody Labeling Kit (Life Technologies) ...
-
No products found
because this supplier's products are not listed.
Michelle A. Johnson, et al.,
bioRxiv - Neuroscience 2021
Quote:
... Cell lysate was immunoprecipitated with Magstrep Type3 beads (IBA Lifesciences; 2-4090-002) overnight with end/end rocking at 4°C following the protocol provided by the manufacturer ...
-
No products found
because this supplier's products are not listed.
Stephen Graham, et al.,
bioRxiv - Immunology 2020
Quote:
... The virus-monoclonal antibody mixture was added (2 mL) to Vero cells in a 6-well plate (Greiner). After infecting the cells for 1 hour at 37°C ...
-
No products found
because this supplier's products are not listed.
Hsiang-i Tsai, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... Recombinant human interleukin-2 (2 ng/ml, IL-2, Peprotech). Ficoll® lymphocyte separation medium (LSM ...
-
No products found
because this supplier's products are not listed.
Yijun Zhang, Joachim Seemann,
bioRxiv - Cell Biology 2020
Quote:
... cleared lysates were mixed with 2 μg mouse monoclonal anti-GRASP55 IgG or 2 μg mouse gamma globulin (Jackson ImmunoResearch) for 60 min at 4°C and incubated with protein A/G-Sepharose (Santa Cruz Biotechnology ...
-
No products found
because this supplier's products are not listed.
Tanay Bhatt, et al.,
bioRxiv - Pathology 2022
Quote:
About 0.1 to 0.2 MOI (Multiplicity of Infection) equivalent of VSV-G and pseudotype SARS-CoV-2 spike virus particles were treated with recombinant LL37 (Tocris) and niacinamide (vitamin B3 ...
-
No products found
because this supplier's products are not listed.
Joseph M Gibbons, et al.,
bioRxiv - Microbiology 2019
Quote:
... Virus-like particles (VLPs) were produced by linear polyethylenimine 25K (Polysciences) transfection of HEK-293T ...
-
No products found
because this supplier's products are not listed.
William B. Klimstra, et al.,
bioRxiv - Microbiology 2020
Quote:
... 2 ml/well of virus growth medium containing 0.1% (w/v) immunodiffusion agarose (MP Biomedicals) was added and incubation was continued for 96 hours ...
-
No products found
because this supplier's products are not listed.
Sebastian Tacke, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... plain grids (Quantifoil 2/2) were transferred to the microscope ...
-
No products found
because this supplier's products are not listed.
Bruno Vaz, et al.,
bioRxiv - Immunology 2020
Quote:
Human APCs were pre-pulsed for 4h at 37 °C with 1:50 antigen mix (HBV, influenza and CMV lysates; bulk telomere measurements) or 1:50 cytomegalovirus (CMV)-lysates (Zeptometrix Corporation; IF-FISH studies). APC-T cell conjugates were then formed by adding autologous CD27+ CD28+ CD3+ CD4+ T cells in a 3:1 ratio ...
-
No products found
because this supplier's products are not listed.
Zeqing Wang, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Antigen retrieval was performed with Antigen Retrieval Solution (Solarbio, C1035) before the conventional immunostaining procedure.
-
No products found
because this supplier's products are not listed.
Federico Giannitti, et al.,
bioRxiv - Pathology 2022
Quote:
... and a mouse monoclonal antibody raised against Simian Virus-40 (SV-40, Macaca mulatta polyomavirus-1, Betapolyomavirus macacae) LT antigen (CalBiochem®, Clone PAb416, Sigma-Aldrich) was used as primary antibody at a 1:200 dilution ...
-
No products found
because this supplier's products are not listed.
Sinem Yılmaz, et al.,
bioRxiv - Cell Biology 2022
Quote:
... The nuclear lysates were subjected to Nrf-2 Transcription Factor Assay (Cayman Chemical, US, 600590) to evaluate the effect of CA on the nuclear Nrf-2 activity ...
-
No products found
because this supplier's products are not listed.
Mingming Chen, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... the lysates containing 10 µg of total RNA were treated with 2 U/µg RNase I (Lucigen) at 25°C for 45 min ...
-
No products found
because this supplier's products are not listed.
Eugenia Zah, et al.,
bioRxiv - Bioengineering 2020
Quote:
... The lysate supernatant was ultracentrifuged with iodixanol (OptiPrep; StemCell Technologies) density gradient solutions (54% ...
-
No products found
because this supplier's products are not listed.
BI Reinfeld, et al.,
bioRxiv - Cancer Biology 2020
Quote:
PyMT GEMM mice were bred by crossing male transgenic mice expressing the polyoma virus middle T antigen (PyMT) oncoprotein under the MMTV-LTR (Jackson Laboratory 022974) with wildtype females on a similar B6/FVB mixed background ...
-
No products found
because this supplier's products are not listed.
Pin-Ji Lei, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... we inoculated 1×105 PFU UV-inactivated PR8 influenza virus + 2μg Anti-CD40 (BioXCell, Cat: BE0016-2) + 2μg poly I:C (InvivoGen ...
-
No products found
because this supplier's products are not listed.
W.J. Venema, et al.,
bioRxiv - Immunology 2021
Quote:
... S-antigen (α-mGFP, TA180076, Origene, to detect the fusion protein S-antigen-GFP ...
-
No products found
because this supplier's products are not listed.
Victoria L. Corbit, et al.,
bioRxiv - Neuroscience 2020
Quote:
... Virus was injected using a syringe pump (Harvard Apparatus) fitted with a syringe (Hamilton ...
-
No products found
because this supplier's products are not listed.
Rhiannon R. Penkert, et al.,
bioRxiv - Cell Biology 2021
Quote:
... GST lysates were incubated with glutathione agarose resin (GoldBio; 50 μL resin per 0.5-1.5 mL of lysate) for 30 min at 4°C and then washed 6x (3x quick washes ...
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No products found
because this supplier's products are not listed.
Timothy P. Sheahan, et al.,
bioRxiv - Microbiology 2020
Quote:
... nanoluciferase expression as a surrogate for virus replication was quantitated on a Spectramax (Molecular Devices) plate reader according to the manufacturer’s instructions (Promega ...
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No products found
because this supplier's products are not listed.
Matthew T. Birnie, et al.,
bioRxiv - Neuroscience 2022
Quote:
Virus labeled sections were scanned under a 10x objective of a fluorescent microscope (Olympus BX 61) equipped with a high-sensitivity CCD camera (Nikon Eclipse E400) ...
-
No products found
because this supplier's products are not listed.
Jianlong Zhai, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... the antigen-antibody complexes were detected using Odyssey XF (LI-COR, USA). The signal intensities of target bands were analyzed using ImageJ software (National Institutes of Health ...
-
No products found
because this supplier's products are not listed.
Huan Chen, et al.,
bioRxiv - Microbiology 2021
Quote:
... the co-immunoprecipitation sample and 2% whole-cell lysates were analyzed by SDS-PAGE and transferred to a polyvinylidene difluoride (PVDF) membrane (Pall Corp.). The membrane was blocked in 3% skim milk in phosphate buffered saline with Tween 20 (PBST ...
-
No products found
because this supplier's products are not listed.
Dongying Chen, et al.,
bioRxiv - Developmental Biology 2024
Quote:
... To harvest protein lysate from IBIDI µSlide ...
-
No products found
because this supplier's products are not listed.
David Ollitrault, et al.,
bioRxiv - Cell Biology 2021
Quote:
... cells were placed for 2 days in Endothelial Cell Growth Medium 2 (EGM-2; PromoCell) supplemented with 0.5 ng/ml of vascular endothelial growth factor 165 (VEGF) ...
-
No products found
because this supplier's products are not listed.
Akira Kitamura, et al.,
bioRxiv - Biophysics 2023
Quote:
... FCCS measurements of the lysates were performed using an LSM510 META ConfoCor3 system (Carl Zeiss, Jena, Germany) equipped with a C-Apochromat 40×/1.2NA W Korr ...
-
No products found
because this supplier's products are not listed.
Jiajia Chen, et al.,
bioRxiv - Developmental Biology 2022
Quote:
... The anti-Smed-FBL-2 antibody (antigen aa 2-19, Abclonal Inc.) was used at a 1:500 dilution after worms were bleached and permeabilized ...
-
No products found
because this supplier's products are not listed.
Dessislava Malinova, et al.,
bioRxiv - Immunology 2020
Quote:
Model antigens used in solution or on PMS were goat anti-mouse Igκ F(ab’)2 (Southern Biotech) for mouse splenic B cells and goat F(ab’)2 anti-human Fc5μ (Jackson Immunoresearch ...
-
No products found
because this supplier's products are not listed.
Ellen Van Gulck, et al.,
bioRxiv - Cell Biology 2023
Quote:
... 48h post transfection virus was collected and concentrated with PEG Virus Precipitation Kit (BioVision Inc, USA) according to the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Andrew Sawyer, et al.,
bioRxiv - Immunology 2022
Quote:
... Antigen retrieval was performed by boiling samples to 100°C for 15 minutes in pH 9 Antigen retrieval buffer (Akoya). Tissues were incubated for 45 minutes at room temperature with primary antibodies specific for CD20 (L26 ...
-
No products found
because this supplier's products are not listed.
Melle Holwerda, et al.,
bioRxiv - Microbiology 2020
Quote:
... SARS-CoV-2 antigen-positive cells were detected using a rabbit polyclonal anti-SARS-CoV nucleocapsid protein (Rockland, 200-401-A50) and a secondary Alexa Fluor® 488-labeled donkey anti-Rabbit IgG (H+L ...
-
No products found
because this supplier's products are not listed.
Lena Street, et al.,
bioRxiv - Systems Biology 2023
Quote:
... 2 mg of cell lysate at 20 mg/mL was loaded onto a Yarra SEC-4000 column (Phenomenex 00H-4514-K0 ...
-
No products found
because this supplier's products are not listed.
Yang S. Chen, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... Lysates were sonicated by Covaris Sonicator to ~500 bp fragments (130 μL/tube ...
-
No products found
because this supplier's products are not listed.
Samantha Mar, et al.,
bioRxiv - Developmental Biology 2023
Quote:
... heated in antigen retrieval buffer (0.433% v/v citraconic anhydride 98% in dH2O, Alfa Aesar), and blocked for 30 minutes in 5% v/v horse serum in PBS ...
-
No products found
because this supplier's products are not listed.
Anniek Zaalberg, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... Lysates were sonicated (EpiShear Probe Sonicatore, Active Motif) for 10 cycles with one second intervals and a 20% amplitude using ...
-
No products found
because this supplier's products are not listed.
Leena Hussein Bajrai, et al.,
bioRxiv - Microbiology 2021
Quote:
... the plates were analyzed by observing virus-induced CPE by light microscope (Nikon-ECLIPSE-Ti), and plaque formation was determined by crystal violet staining (C0775 ...
-
No products found
because this supplier's products are not listed.
Adrià Sogues, et al.,
bioRxiv - Microbiology 2019
Quote:
... A 10 mM lipids chloroform solution made of an 8:2 mixture of 1-palmitoyl-2-oleoyl phosphatidylcholine (POPC) and 1-palmitoyl-2-oleoylglycero-3-phosphoglycerol (POPG) (Avanti Polar Lipids). Chloroform was removed by evaporation under vacuum conditions and the dried phospholipid film was resuspended in a mixture of diethyl ether and buffer (25 mM Hepes pH 7.4 ...
-
No products found
because this supplier's products are not listed.
Teresa R. Wagner, et al.,
bioRxiv - Immunology 2023
Quote:
... purified antigen (Acrobiosystems) was reacted with Sulfo-NHS-LC-LC-Biotin (Thermo Fisher Scientific ...
-
No products found
because this supplier's products are not listed.
Nitin Hingankar, et al.,
bioRxiv - Immunology 2021
Quote:
... The avi-tagged SARS-CoV-2 RBD antigen was first labeled with biotin (Avidity, BirA500) was subsequently coupled to streptavidin-PE and streptavidin-APC (BD Biosciences ...
-
No products found
because this supplier's products are not listed.
Nils H. Rustmeier, et al.,
bioRxiv - Biochemistry 2023
Quote:
... and 20 mM Tf antigen (TCI Europe), at 20 °C for half an hour ...
-
No products found
because this supplier's products are not listed.
Benjamin Gan-Or, Michael London,
bioRxiv - Neuroscience 2022
Quote:
... Virus-containing solution was slowly injected (1-2 nl/sec) through a quartz pipette (pulled on P2000, Sutter instruments,) using a Nanoject 3 system (Drummond Scientific Company ...
-
No products found
because this supplier's products are not listed.
K Pilarzyk, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Lysates for all WTs within a cohort (Cohort 1: 2F + 3M; Cohort 2: 3M; Cohort 3: 4F; Cohort 4: 6M), and all KOs within a cohort were pooled (Cohort 1 ...
-
No products found
because this supplier's products are not listed.
Jill B. Graham, et al.,
bioRxiv - Cell Biology 2019
Quote:
... Antibodies directed toward the following antigens were also purchased: calnexin (Enzo Life Sciences); calreticulin (ThermoFisher Scientific) ...
-
No products found
because this supplier's products are not listed.
Arthur Flohr Svendsen, et al.,
bioRxiv - Cell Biology 2021
Quote:
... Virus was produced by transfecting Platinum-E retroviral packaging cells (Cell Biolabs) according to the manufacturer’s protocol ...
-
No products found
because this supplier's products are not listed.
Michael Bauer, et al.,
bioRxiv - Microbiology 2020
Quote:
... Genome-labeled AdV was produced by growing the virus in A549 cells in the presence of 2.5 μM EdC (5-ethynyl-2’-deoxycytidine, Jena Biosciences) as described 52 ...
-
No products found
because this supplier's products are not listed.
CL Esposito, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... 1 mM 2’F-Py (2’F-2’-dCTP and 2’F-2’-dUTP, TriLink Biotechnologies), 1 mM ATP ...