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Cat# G209,
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Oliver Hsia, et al.,
bioRxiv - Biochemistry 2023
Quote:
... Lysate was filtered through a BioPrepNylon Matrix Filter (BioDesign) then incubated with 1 mL Ni-NTA resin per litre culture for 1 hour ...
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No products found
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Zhijun Qiu, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... followed by “antigen loading” with the addition of the ovalbumin peptide (OVA-323-339) (Cat# 12787-01, Bio-Synthesis) for 3 hours ...
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No products found
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Maayan Karlinski Zur, et al.,
bioRxiv - Developmental Biology 2024
Quote:
... The lysates were transferred into soft tissue homogenizing CK14 tubes (Bertin Corp), placed in a homogenizer shaker at 400bps for 2 sec ...
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No products found
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Wenhui Qiao, et al.,
bioRxiv - Neuroscience 2021
Quote:
... Aβ oligomers in TBS and TBSX lysates were detected by commercial kits (Biosensis, BEK-2215-2P). sAPPa ...
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No products found
because this supplier's products are not listed.
Meghan D. J. Bragdon, et al.,
bioRxiv - Synthetic Biology 2022
Quote:
Yeast colonies were picked from plates and cultured overnight in 2 mL liquid synthetic defined (SD) media with appropriate auxotrophic dropouts (Sunrise Science Products). Cultures were diluted 1:50 into 500 µL of synthetic complete (SC ...
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No products found
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Livia Mazzini, et al.,
bioRxiv - Immunology 2020
Quote:
... Human IgM anti-SARS-CoV-2 Spike (S1) Antibody CR3022 (Native Antigen, Oxford, UK) and anti-Spike RBD (SARS-CoV-2/COVID 19) human monoclonal antibody (eEnzyme, Gaithersburg, USA) were used as positive controls in ELISA ...
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No products found
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Xiaozheng Xu, et al.,
bioRxiv - Cell Biology 2019
Quote:
... SEE (#ET404) super antigen was purchased from Toxin Technology. CD28 monoclonal antibody (#16-0289-85) ...
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No products found
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Nozomi Shiwa-Sudo, et al.,
bioRxiv - Microbiology 2022
Quote:
... SARS-CoV-2 antigens were detected using a polymer-based detection system (Nichirei-Histofine Simple stain MAX PO; Nichirei Biosciences, Inc., Tokyo, Japan), and an in-house rabbit anti-SARS-CoV-2 N antibody was used as the primary antibody ...
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No products found
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Julia Bitencourt, et al.,
bioRxiv - Immunology 2021
Quote:
... The antigens used were purified protein derivative (PPD) from M.tb (Statens Serum Institut (SSI), Denmark ...
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No products found
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Hongyan Sui, et al.,
bioRxiv - Immunology 2019
Quote:
HSV-1 (MacIntyre strain) and Sendai virus (SeV) were obtained from Advanced Biotechnologies Inc ...
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No products found
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Alyssa N Coyne, et al.,
bioRxiv - Neuroscience 2020
Quote:
Two rabbits were immunized with the peptide antigen C-Ahx-(GP)8-amide (21st Century Biochemicals). Specificity of antiserum versus pre-immune serum was verified by peptide dot blot and Western blot of HEK cells overexpressing GFP-tagged dipeptide repeat proteins (plasmids kindly provided by L ...
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No products found
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Susanne A. Wycislo, et al.,
bioRxiv - Cell Biology 2020
Quote:
... Equivalent amounts of “light”-labeled control and “heavy”-labeled GFP–containing lysates or “light”-labeled control and “heavy”-labeled GFP–containing lysates were incubated (separately) with GFP-Trap agarose beads (Allele Biotechnology, San Diego, CA) for 30 min at 4°C ...
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No products found
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James S. Dhaliwal, et al.,
bioRxiv - Cell Biology 2020
Quote:
... Cleared lysates were then incubated with α8-oxoG (QED Bioscience) at 4°C for 1 hr to capture ORBs ...
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No products found
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Assunta Senatore, et al.,
bioRxiv - Neuroscience 2020
Quote:
... The following antigens were coated on separate 384-well ELISA plates: anti-Fd antibody (The Binding Site GmbH) 1:1000 in PBS ...
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No products found
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Camille L. Duran, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... and antigen retrieval was performed in 1 mM EDTA (pH 8.0) or 1x citrate buffer (pH 6.0) (Diagnostic BioSystems) at 97°C for 20 minutes in a conventional steamer ...
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No products found
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Marie A Brunet, et al.,
bioRxiv - Genetics 2020
Quote:
... Human tissue lysates for altFUS endogenous expression were purchased from Zyagen Laboratories (San Diego ...
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No products found
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Emily S. Norton, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... lysates were clicked to 10 μM DBCO-S-S-PEG3-biotin (BroadPharm) for 6 hours at room temperature ...
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No products found
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Avital Licht-Murava, et al.,
bioRxiv - Neuroscience 2022
Quote:
... per ml culture medium at DIV 8 using Lipofectamine 3000 5 h before infection with vesicular stomatitis virus (VSV) at 100 MOI or with adenovirus-eGFP (Ad5CMV-eGFP, lot #ad3586, Viral Vector Core Facility, Carver College of Medicine ...
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No products found
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Sara Al Rawi, et al.,
bioRxiv - Neuroscience 2021
Quote:
Fbxo7 was immunoprecipitated from cell lysates using 2μl of anti-Fbxo7 antibody (Aviva), or isotype matched control ...
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No products found
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Ni Zeng, et al.,
bioRxiv - Immunology 2021
Quote:
... Splenocytes depleted of T cells were used as antigen-presenting cells (APCs)/feeder cells and were irradiated in RS2000 (Rad Source Technologies) with a total dose of 30 Gy within a period of 10 mins ...
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No products found
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Carlo Cervia, et al.,
bioRxiv - Immunology 2020
Quote:
... and serum was pre-diluted 1:20 in sample buffer and transferred to antigen-coated 1536-well assay plates using acoustic dispensing technology (Labcyte ECHO 555) with serial dilutions ranging from 1:5–1:640 (mucosal samples ...
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A DNA sequence encoding the extracellular domain of mouse Cd33 (Met 1 - Glu 240) (FITC...
Cat# Cd33-6889MF,
50ug , USD $1298
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Yan Qi, et al.,
bioRxiv - Molecular Biology 2024
Quote:
... the presence of anti- Ad and anti-hemagglutinin antibodies was measured by ELISA against a purified Ad6 virus preparation (Greffex, Inc.) and a recombinant hemagglutinin protein (Creative Biomart). In the first case ...
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No products found
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Jasper Che-Yung Chien, et al.,
bioRxiv - Genetics 2020
Quote:
... and solutions of B02 (2×10-2 M; Abmole) and CAY10566 (CAY ...
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Gab-Chol Choi, et al.,
bioRxiv - Bioengineering 2020
Quote:
... and bone morphogenetic protein 2 (BMP-2) (1:200, orb251474, Biorbyt) primary antibodies at 4°C ...
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No products found
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Eva Mittler, et al.,
bioRxiv - Microbiology 2020
Quote:
... cleared U2OS-Cas9 lysates were pre-incubated with the protease inhibitor E-64 (20 μM; Peptides International) for 20 min at room temperature (when indicated) ...
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No products found
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Julieta Ramirez, et al.,
bioRxiv - Genomics 2023
Quote:
Whole cell lysates were separated on a 5% SDS/PAGE containing 20 μm Phos-tag (NARD Institute), followed by western blotting with anti-CTCF antibody.
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No products found
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Sana Charaoui-Boukerzaza, et al.,
bioRxiv - Microbiology 2019
Quote:
... The count of viable bacteria was carried out by plating serial dilutions of the lysates on blood agar (43041, bioMérieux) using an automatic seeder (EasySpiral Dilute, Interscience). The calculation of the bacterial loads was performed after incubation at 37°C for 24 h ...
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No products found
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Brittany G. Seman, et al.,
bioRxiv - Immunology 2019
Quote:
... the culture was supplemented with 100 units of interleukin-2 (IL-2; Shenandoah Biotech, Warwick, PA) or 3×105 CD3/CD28 Dynabeads/well (ThermoFisher Scientific ...
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No products found
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Salime Bazban-Shotorbani, et al.,
bioRxiv - Bioengineering 2021
Quote:
... MAL-PEG-NHS (2 kDa) and m-PEG-NHS (2 kDa) were obtained from Nanocs (USA).
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No products found
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Etai Sapoznik, et al.,
bioRxiv - Biophysics 2020
Quote:
... #1.5 coverslips (0420-0323-2, Bioptechs) were washed at room temperature in solution consisting of 1:1 (vol/vol ...
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No products found
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Raul Jobava, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... harringtonine (2 μg/mL) (LKT Laboratories, H0169), sephin 1 (Apexbio,A8708 ...
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No products found
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Javier Martínez Pacheco, et al.,
bioRxiv - Plant Biology 2022
Quote:
... Rabbit AtTOR polyclonal antibodies (Abiocode, R2854-2), rabbit polyclonal S6K1/2 antibodies (Agrisera ...
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No products found
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Diego A. Vargas-Blanco, et al.,
bioRxiv - Microbiology 2019
Quote:
... transferred to 2 mL disruption tubes (OPS Diagnostics 100 μm zirconium lysing matrix ...
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No products found
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Angela Criscuolo, et al.,
bioRxiv - Biochemistry 2021
Quote:
... 1,2-Dipalmitate-3-linoleate-glycerol (TG 16:0/16:0/18:2) and cholesteryl linoleate (CE 18:2) were purchased from Larodan (Solna, Sweden). Oxylipin standards (15(S)-hydroperoxy eicosatetraenoic acid ...
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No products found
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Wentao Yu, et al.,
bioRxiv - Bioengineering 2021
Quote:
... An optical long-pass filter can be placed before the tube lens to further reduce the backscattered UV light. A custom-built 2-axis angle adjustable platform (Supplementary Fig. 2) under the vibratome (VF-700-0Z, Precisionary Instruments Inc.) ensures the focal plane of the objective lens is parallel with the surface of the sample sectioned by the vibratome ...
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No products found
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B. H. Abuaita, et al.,
bioRxiv - Immunology 2019
Quote:
... CASPASE-2 FAM-VDVAD-FMK FLICA substrate (ImmunoChemistry Technologies) for 1h at 37°C or 2 μM CellEvant CASPASE-3/7 Green detection reagent (Thermo Fisher ...
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No products found
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Chengfeng Xiao, Shuang Qiu,
bioRxiv - Genetics 2020
Quote:
... separated in 2 % gel of agarose (A87-500G, FroggaBio), and visualized with AlphaImager 2200 Gel Documentation and Image Analysis System (Alpha Innotech).
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No products found
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Ketakee Ghate, et al.,
bioRxiv - Neuroscience 2020
Quote:
... 200 μl of 2 mg/ml sulfo-SANPAH (Covachem) was added to the gels and allowed to coat the gels for 1 hr at 5 to 8 cm distance from a portable UV lamp (365 nm ...
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No products found
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Diwakar Turaga, et al.,
bioRxiv - Bioengineering 2020
Quote:
Cardiac microtissues stained for GATA4 and labeled with Hoechst were suspended in size 2 glass capillaries (Zeiss; ∼1mm inner diameter) in 2% low-melt agarose (made up in PBS; IBI Scientific, Dubuque, IA) immediately prior to imaging (Supplementary Figure 1) ...
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No products found
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Aleksi Isomursu, et al.,
bioRxiv - Biophysics 2020
Quote:
Cells that were collected for protein lysates were cultured on commercial hydrogel-coated 6-well plates (Matrigen, SW6-EC-0.5/SW6-EC-8/SW6-EC-50). These gels were similarly coated with 10 μg/ml of fibronectin before use.
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No products found
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Sara M. Eslami, et al.,
bioRxiv - Bioengineering 2023
Quote:
... Quartz spectrophotometer cell (Starna Cells, cat. no. 1-Q-2) and measured using an Olis Cary-16 circular dichroism spectrometer ...
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No products found
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Aurelie Velay, et al.,
bioRxiv - Microbiology 2020
Quote:
... ELISA anti-SARS-CoV-2 IgA and IgG (Euroimmun, Lübeck, Germany) and (2) ELISA-2: EDI™ novel coronavirus COVID-19 IgM and IgG (Epitope Diagnostics, San Diego, CA, USA). Technical characteristics of the assays are summarized in the Supplementary data (Table S1) ...
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No products found
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Jesse R. Holt, et al.,
bioRxiv - Cell Biology 2020
Quote:
... Keratinocytes were imaged following at least 2 days in Cnt-Pr-D (CellnTec) differentiation media.
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No products found
because this supplier's products are not listed.
Stephen C. Gironda, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Microdialysis probes (2 mm; 38 kDa molecular weight cut off; BR-style; BASi) were inserted into the guide cannula ...
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No products found
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Shota Yoshida, et al.,
bioRxiv - Immunology 2020
Quote:
... Synthetic SARS-CoV-2 peptides (Appendix Table S2; Peptide Institute Inc., Osaka, Japan) were diluted to a concentration of 10 μg/ml and coated at 500 ng/well ...
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No products found
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Nick Quinn-Bohmann, et al.,
bioRxiv - Systems Biology 2023
Quote:
Fecal samples were collected in 1200 mL 2-piece specimen collectors (Medline, USA) in the Public Health Science Division of the Fred Hutchinson Cancer Center (IRB Protocol number 10961 ...
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No products found
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Hyunjoon Kim, Soohyun Jang, Young-suk Lee,
bioRxiv - Molecular Biology 2021
Quote:
... cells were selected with medium containing 1∼2 μg/ml puromycin (AG Scientific #P-1033) until non-transduced cells became completely dead ...
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No products found
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Rosalba Perrone, et al.,
bioRxiv - Developmental Biology 2023
Quote:
... Slides were incubated with the following protocol: 2 minutes in Modified Mayer’s Hematoxylin (StatLab, McKinney, TX, HXMMHGAL), 2 washes with dH20 ...
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No products found
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Bryana N. Harris, et al.,
bioRxiv - Systems Biology 2022
Quote:
Cardiac myocytes were isolated from 1-2-day-old Sprague-Dawley rats using a Neomyt isolation kit (Cellutron, USA). The cells were cultured in plating media (low-glucose Dulbecco’s modified eagle media (DMEM) ...
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Superoxide dismutase (SOD) is an antioxidant enzyme involved in the defense system against...
Cat# PBCA1050,
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Pragya D Yadav, et al.,
bioRxiv - Microbiology 2021
Quote:
... Pseudovirus (an HIV-based luciferase expressing lentivirus pseudotyped with SARS-CoV-2 full length S protein) was obtained from Creative Biogene. One step luciferase assay kit from BPS Bioscience was used for detection ...