-
No products found
because this supplier's products are not listed.
Ryo Okuda, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... anti-Trefoil Factor 3 (1:100, Abcam, ab108599), anti-Lipocalin-2 (1:100 ...
-
No products found
because this supplier's products are not listed.
Gaelle Noel, et al.,
bioRxiv - Immunology 2021
Quote:
... TFF3 (rabbit polyclonal [pAb], Millipore Sigma), lysozyme EC 3.2.1.17 (rabbit pAb ...
-
No products found
because this supplier's products are not listed.
Julie Chassagne, et al.,
bioRxiv - Pathology 2024
Quote:
... ribosomal protein L32 (RPL32) for dogs and 18S ribosomal RNA (Rn18s) for mice on Quant Studio 3 (Applied Biosystems, Life Technologies) using Master Mix TaqMan Fast Advanced (Life Technologies) ...
-
No products found
because this supplier's products are not listed.
Aditya Arora, et al.,
bioRxiv - Cell Biology 2020
Quote:
... and 4% DOGS-NTA-Ni (Avanti Polar Lipids) were then deposited using Small Unilamellar Vesicle (SUV ...
-
No products found
because this supplier's products are not listed.
Samuel A. Miller, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... 1μg/ml anti-TFF3 (R&D Systems, MAB4407) or IgG was added to the media for 5 minutes ...
-
No products found
because this supplier's products are not listed.
Ilkka Fagerlund, et al.,
bioRxiv - Neuroscience 2020
Quote:
... 10 ng/mL IL-3 and stem cell factor (SCF; all Peprotech). From now on ...
-
No products found
because this supplier's products are not listed.
Abigail G. Harrell, et al.,
bioRxiv - Bioengineering 2023
Quote:
... 3 ng/mL human basic fibroblast growth factor (Corning), 0.135 ng/mL human vascular endothelial growth factor (Sigma) ...
-
No products found
because this supplier's products are not listed.
Heng Xia, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... Protein concentration of VSW-3 RNAP was determined by Bradford protein quantitative kit (Bio-rad), and protein purity and concentration were analyzed along with T7 RNAP (New England Biolabs ...
-
No products found
because this supplier's products are not listed.
Yohey Terai, et al.,
bioRxiv - Evolutionary Biology 2023
Quote:
... the DNAs of one Jomon dog (MD1) and three late 8th century dogs (SWD_2, SWD_5, and SWD_7) were treated by USER enzyme (NEB) for uracil removal ...
-
No products found
because this supplier's products are not listed.
Heather Wilson-Robles, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... A total of 3-5 mL (depending on the size of the dog) was placed in K2-EDTA primed (lavender top) tubes (Becton, Dickinson and Company, Franklin Lakes, NJ). Samples were centrifuged within one hour of collection at room temperature at 3000xg for 10 min(26).
-
No products found
because this supplier's products are not listed.
Antoni J. Gralak, et al.,
bioRxiv - Genomics 2024
Quote:
Transcription factors were provided as EGFP fusion proteins in pF3A WG (Promega) by the Codebook/GRECO-BIT collaboration16 ...
-
No products found
because this supplier's products are not listed.
Jia Chen, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Protein concentration was estimated using BCA protein assay kit (Merck, 71285-3, Germany).
-
No products found
because this supplier's products are not listed.
Rasmi Abu-Helu, et al.,
bioRxiv - Microbiology 2024
Quote:
DNA was extracted from dogs’ feces using QIAamp PowerFecal Pro DNA Kit (Qiagen), following manufacturers’ instructions ...
-
No products found
because this supplier's products are not listed.
Sara I Hernandez, et al.,
bioRxiv - Neuroscience 2024
Quote:
... and five control dogs were evaluated with antibodies (Abs) against CD3 (T cells) (M7254, Ms mAb; Agilent Dako), CD79b (B cells ...
-
No products found
because this supplier's products are not listed.
Xiaopeng Tang, et al.,
bioRxiv - Immunology 2020
Quote:
... anti-phospho-interferon regulatory factor 3 (IRF3) (1:1□000 dilution, 29047S, Cell Signaling Technology, USA), anti-total-IRF3 (1:1□000 dilution ...
-
No products found
because this supplier's products are not listed.
Maria M. Steele, et al.,
bioRxiv - Immunology 2022
Quote:
... Transcription factor staining was performed using True-Nuclear Transcription Factor Buffer Set (Biolegend) according to manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Temistocles Molinar Jr., et al.,
bioRxiv - Molecular Biology 2022
Quote:
... alpha-factor peptide (GenScript) was added to a final concentration of 5 µg/ml for 1.5 hours ...
-
No products found
because this supplier's products are not listed.
David Brena, et al.,
bioRxiv - Developmental Biology 2020
Quote:
GST fusion proteins were generated using pGEX5x.3 (GE Healthcare) as vector ...
-
No products found
because this supplier's products are not listed.
Benjamin Furtwängler, et al.,
bioRxiv - Biochemistry 2021
Quote:
... supplemented with growth factors (Miltenyi Biotec, IL-3, IL-6 and G-CSF (10 ng/mL), h-SCF and FLt3-L (50 ng/mL) ...
-
No products found
because this supplier's products are not listed.
Yuhao Wang, et al.,
bioRxiv - Cell Biology 2023
Quote:
... Both GFP11-tagged Fluc proteins and GEM transcriptional factor (cloned from pJW1663, Addgene plasmid #112037) were stably integrated into yeast genome ...
-
No products found
because this supplier's products are not listed.
Abhishek Shivappagowdar, et al.,
bioRxiv - Cell Biology 2020
Quote:
... supplemented with growth factors (Lonza).
-
No products found
because this supplier's products are not listed.
Marie-France Dorion, et al.,
bioRxiv - Neuroscience 2023
Quote:
... octamer-binding transcription factor 3/4 (OCT3/4; #sc-8628, Santa Cruz Biotechnologies at 1:500), lysosomal-associated membrane protein 1 (LAMP1 ...
-
No products found
because this supplier's products are not listed.
Grace Freke, et al.,
bioRxiv - Genetics 2023
Quote:
... Bound protein/protein complexes were washed 3 times ((1x TBS, 0.5% Nonident P40 (Roche), 1% phosphatase inhibitor cocktail 2 and 3 (Sigma)) ...
-
No products found
because this supplier's products are not listed.
Ramon Martinez-Marmol, et al.,
bioRxiv - Neuroscience 2021
Quote:
... brain-derived neurotrophic factor (BDNF, #78133; StemCell Technologies), ascorbic acid (200 μM ...
-
No products found
because this supplier's products are not listed.
Juan Martín D’Ambrosio, et al.,
bioRxiv - Cell Biology 2020
Quote:
For testing protein-protein interactions Matchmaker™ GAL4 Two-Hybrid System 3 was used according to the manufacturer’s instructions (Clontech). Briefly ...
-
No products found
because this supplier's products are not listed.
Laura de las Heras-García, Olatz Pampliega,
bioRxiv - Neuroscience 2022
Quote:
... Primary antibodies included: ADP ribosylation factor factor-like protein 13 B (Arl13b) (#17711-1-AP, Proteintech), Cathepsin D (#ab75852 ...
-
No products found
because this supplier's products are not listed.
Isain Zapata, et al.,
bioRxiv - Genetics 2021
Quote:
... dataset contained 444 subjects from 29 dog breeds genotyped for ∼175,000 SNPs (Illumina CanineHD array)14 ...
-
No products found
because this supplier's products are not listed.
Brooke K. Hayes, et al.,
bioRxiv - Microbiology 2023
Quote:
... 3 μL of 3 mg/mL of protein was added to glow discharged UltrAuFoil holey gold grid (Quantifoil GmbH) using a Vitrobot Mark IV (Thermofisher ...
-
No products found
because this supplier's products are not listed.
Anusha B. Gopalan, et al.,
bioRxiv - Developmental Biology 2022
Quote:
... and 1% Pen/Strep) +2iLIF (10 ng/ml leukemia inhibitory factor + 3 μM CHIR99021 + 1 μM PD0325901 from Tocris, Cat. # 4423 and 4192, respectively). The cells were grown on 0.1% gelatin-coated dishes and usually seeded at a density of 0.5–1.5 × 104/cm2 ...
-
No products found
because this supplier's products are not listed.
Mark R. MacRae, et al.,
bioRxiv - Biophysics 2022
Quote:
... the purified and concentrated protein was incubated with 1:3 protein to biotin ratio of 1mM NHS-PEG4-Biotin (VWR #PI21362) for 1 hr at room temperature ...
-
No products found
because this supplier's products are not listed.
Julia Duque, et al.,
bioRxiv - Biophysics 2023
Quote:
... we purchased shRNAs targeting dog desmoplakin in a lentiviral vector (V3LHS 302846 and 302847, pGIPZ vector, Horizon discovery, Perkin Elmer, UK). We generated lentiviral particles following manufacturer instructions ...
-
No products found
because this supplier's products are not listed.
Isha Ralhan, et al.,
bioRxiv - Cell Biology 2022
Quote:
... The supernatant was centrifuged at 300,000×g at 4°C for 3 h in a TLA-110 fixed angle rotor (k-factor 13) (Beckman Coulter). The pellet was resuspended in 10 µL PBS of which 3 µL was adsorbed onto glow-discharged 2 nm carbon layer coated Quantifoil R1.2/1.3 400 mesh gold grids ...
-
No products found
because this supplier's products are not listed.
Sunnyjoy Dupuis, et al.,
bioRxiv - Systems Biology 2024
Quote:
20 mL of culture from each of 3 experimental replicates (n = 3) was collected in Protein LoBind tubes (Eppendorf, Hamburg, Germany) by centrifugation at 1650 xg for 10 min ...
-
No products found
because this supplier's products are not listed.
Matthew R Hathaway, et al.,
bioRxiv - Cancer Biology 2024
Quote:
... All cell lines were grown as spheroids and media supplemented with 0.2μg/mL each Human Epidermal Growth Factor Protein (Sino Biological, 10605-HNAE-1) and Human Recombinant bFGF/FGF2 Protein (Sino Biological ...
-
No products found
because this supplier's products are not listed.
Maria L. Bonatelli, et al.,
bioRxiv - Microbiology 2022
Quote:
... Proteins were removed by ultrafiltration (3 kDa Vivaspin; Sartorius, Göttingen, Germany). ACE and ANSA hydrolysis activities were quantified in discontinuous HPLC-based assays at 30°C in lysis buffer ...
-
No products found
because this supplier's products are not listed.
Gabriella Fioravanti, et al.,
bioRxiv - Bioengineering 2020
Quote:
... 50 ng/mL nerve growth factor (Envigo NGF 2.5S), or 5nM ...
-
No products found
because this supplier's products are not listed.
Nicholas L. Truex, et al.,
bioRxiv - Immunology 2023
Quote:
... and streptavidin IRdye680 (LI-COR Biosciences; dilution factor: 1/2000). The goat anti-rabbit IRdye680 secondary antibody (LI-COR Biosciences ...
-
No products found
because this supplier's products are not listed.
Dipti Rai, et al.,
bioRxiv - Cell Biology 2022
Quote:
... 2-3 μl of protein was applied to glow-discharged carbon-coated copper grids (EMS; CF-400-Cu) and incubated for 45 s at room temperature ...
-
No products found
because this supplier's products are not listed.
Sangeeta Goswami, et al.,
bioRxiv - Immunology 2022
Quote:
... 3′-3-diaminobenzidine (DAB) substrate (Leica Microsystems) was used as a chromogen followed by hematoxylin counterstain ...
-
No products found
because this supplier's products are not listed.
Camille Ternet, et al.,
bioRxiv - Systems Biology 2022
Quote:
... EGF (epidermal growth factor) or the HIF-Hydroxylase Inhibitor DMOG (Cayman chemical) at different concentration (20 and 200 ng/ml).
-
No products found
because this supplier's products are not listed.
Judit Vágó, et al.,
bioRxiv - Cell Biology 2022
Quote:
... Equal amounts of protein (3 µg) were loaded into 12–230 kDa separation modules (Protein Simple, Bio-Techne ...
-
No products found
because this supplier's products are not listed.
Farès Ousalem, et al.,
bioRxiv - Microbiology 2024
Quote:
Protein samples were injected onto a Yarra 3 µm SEC-2000 (Phenomenex) running at RT in 150 mM NaCl ...
-
No products found
because this supplier's products are not listed.
Deike J. Omnus, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... The final protein sample was concentrated (Pall Advance Centrifugal Device, MWCO 3 kDa), adjusted to 100 µM and stored in aliquots at −80°C ...
-
No products found
because this supplier's products are not listed.
Andrew Dunbar, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... Protein quantification was performed using the Pierce BCA protein assay kit (ThermoScientific) and analyzed on a Cytation 3 plate reader (BioTek). Proteins were separated by NuPAGE 4-12% Bis-Tris Gel and transferred to a nitrocellulose membrane ...
-
No products found
because this supplier's products are not listed.
João Pedro Fonseca, et al.,
bioRxiv - Systems Biology 2020
Quote:
... Measurements for cAMP ELISA and BCA Protein Assay kits were performed in a FlexStation 3 Multi-Mode Microplate Reader (Molecular Devices). The cAMP dose response to light was fit to a hyperbolic equation and confidence intervals were extracted by bootstrapping in a custom Python Script available on GitHub (https://qithub.com/jpfon/cAMP).
-
No products found
because this supplier's products are not listed.
Casey L. Mahoney-Crane, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Sections were developed using ImmPACT-3 3’-diaminobenzidine (DAB; Vector Labs), sequentially dehydrated as previously described (Stoyka et al. ...
-
No products found
because this supplier's products are not listed.
Vidur Garg, et al.,
bioRxiv - Developmental Biology 2023
Quote:
... + 3% donkey serum (Jackson ImmunoResearch)] at room temperature for 15 mins ...
-
No products found
because this supplier's products are not listed.
Renée L. Finnen, et al.,
bioRxiv - Microbiology 2024
Quote:
... Lysates from HaCaT cells grown on two 150 mm dishes and infected with HSV-2 strain 186 viruses carrying pUL21-mCherry fusion proteins at an MOI of 3 were prepared at 18 hpi and immobilized on RFP-TRAP beads (Chromotek, Rosemont, IL) according to manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Annika Ullrich, et al.,
bioRxiv - Pharmacology and Toxicology 2023
Quote:
... and the plasmids for G protein activation (the ratio of receptor/Gα/Gβ/Gγ was 4/1/2/8) using linear polyethyleneimine (PEI, Polysciences, 3:1 PEI:DNA ratio) (65 ...
-
No products found
because this supplier's products are not listed.
John K. Mich, et al.,
bioRxiv - Neuroscience 2023
Quote:
... the following primers were used: (5’-GGTTTCCCGCAGAACCTGAA-3’) and (5’-CCATCGCTCGACCAGTTTAGT-3’) (Jackson Laboratories)