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No products found
because this supplier's products are not listed.
Stuti Mehta, et al.,
bioRxiv - Molecular Biology 2022
Quote:
AAVPrime™ Adeno-associated virus (AAV) Serotype Testing Kit (GeneCopoeia) was used to determine the efficiency of 8 different AAV serotypes in infecting the HCC1187 cell line ...
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No products found
Shuai Yan, et al.,
bioRxiv - Physiology 2023
Quote:
Adeno-associated virus serotype 8 expressing mouse Aig1 and AAV8-GFP control were purchased from Applied Biological Materials (Canada). AAV injection was carried out as previously described57 ...
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No products found
because this supplier's products are not listed.
Sumiko Takao, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... Cleared lysates were incubated with 4 mL glutathione agarose resin slurry (GoldBio) for 1 h at 4° C to capture GST-KIX ...
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No products found
because this supplier's products are not listed.
A.M.P.B Seneviratne, et al.,
bioRxiv - Cell Biology 2022
Quote:
... Lysates were incubated overnight at 4°C with 4-6μg rabbit anti-GFP antibody (Novus Biologicals, NB 600-303) or mouse anti-myc antibody (Sigma M4439 ...
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No products found
because this supplier's products are not listed.
Nitin Raj, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... pre-cleared lysates were incubated with 2-4 µl p53 polyclonal antibody (Leica Biosystems, NCL-L-p53-CM5p) overnight at 4°C on a nutator to allow p53 protein complexes to form ...
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No products found
because this supplier's products are not listed.
Anthony J. Snyder, Andrew T. Abad, Pranav Danthi,
bioRxiv - Microbiology 2021
Quote:
... the virus-resistant cell population was transferred to fresh 150-mm dishes (Greiner Bio-One) and allowed to expand in growth medium supplemented with 2 μg/ml puromycin (InvivoGen ...
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No products found
because this supplier's products are not listed.
Komal Raj Rijal, et al.,
bioRxiv - Microbiology 2020
Quote:
... The data presented in this study represents serological diagnosis using rapid test kit (SD Bioline dengue IgG/IgM antibody up to 2015; and after 2015, SD Bioline dengue duo (dengue NS1 Ag+ IgG/IgM), Korea ...
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No products found
because this supplier's products are not listed.
Shang Geng, Xing Lv, Weiwei Zheng, Tianjun Xu,
bioRxiv - Immunology 2024
Quote:
... The lysate was incubated over night at 4°C with anti-His beads (Solarbio). Anti-His beads were washed subsequently with washing buffer (500 mM NaCl ...
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No products found
because this supplier's products are not listed.
Ellen Van Gulck, et al.,
bioRxiv - Cell Biology 2023
Quote:
... 48h post transfection virus was collected and concentrated with PEG Virus Precipitation Kit (BioVision Inc, USA) according to the manufacturer’s instructions ...
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No products found
because this supplier's products are not listed.
Sandy Bauherr, et al.,
bioRxiv - Microbiology 2019
Quote:
... Cells infected with fluorescently labeled virus were fixed using 4 % paraformaldehyde (PFA) in PBS and observed on a Fluoview FV-1000 confocal microscope (Olympus, Japan) to identify fluorescent spots and record their local environment ...
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No products found
because this supplier's products are not listed.
Anzhela Migur, et al.,
bioRxiv - Plant Biology 2021
Quote:
... Cell lysates were cleared by centrifugation at 13,000 g for 30□min at 4°C and the lysate was filtered through 0.45□ µm Supor-450 filters (Pall). Recombinant proteins were immobilized on a HiTrap Talon crude 1 ml column (GE Healthcare) ...
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No products found
because this supplier's products are not listed.
Kari L. Price, et al.,
bioRxiv - Developmental Biology 2020
Quote:
... Worm lysates from one freeze-thaw cycle were homogenized with a 4:100 mix of β-mercaptoethanol (MP Biomedicals) and sample buffer (NuPAGE LDS 4X Sample Buffer ...
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No products found
because this supplier's products are not listed.
Victoria L. Corbit, et al.,
bioRxiv - Neuroscience 2020
Quote:
... Virus was injected using a syringe pump (Harvard Apparatus) fitted with a syringe (Hamilton ...
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No products found
because this supplier's products are not listed.
Irina V. Chestakova, et al.,
bioRxiv - Microbiology 2023
Quote:
... and H1N1 virus A/California/07/2009 (Sino Biological) using an arbitrary fluorescence cut-off of 6,000 ...
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No products found
because this supplier's products are not listed.
Melina Möller, et al.,
bioRxiv - Biophysics 2021
Quote:
... The pellet was solubilized for 30 minutes at 4°C and cell lysates were purified using the μMacs GFP isolation kit (Miltenyi Biotec) according to the manufacturer’s protocol ...
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No products found
because this supplier's products are not listed.
Moran Brenner, et al.,
bioRxiv - Microbiology 2021
Quote:
... Cold (4°C) lysates were deproteinated with an equal volume of metaphosphoric acid and quantified with a commercial kit (Cayman Chemical), according to the recommendations of the kit manufacturer.
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No products found
because this supplier's products are not listed.
Joshua J. Sims, et al.,
bioRxiv - Genetics 2021
Quote:
... We measured reporter virus transduction activity on a luminometer (BioTek) using the Renilla Glo Kit (Promega ...
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No products found
because this supplier's products are not listed.
Yang S. Chen, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... Lysates were sonicated by Covaris Sonicator to ~500 bp fragments (130 μL/tube ...
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No products found
because this supplier's products are not listed.
Camilla Schinner, et al.,
bioRxiv - Cell Biology 2021
Quote:
... virus particle containing supernatant was collected and enriched using LentiConcentrator (OriGene). Cells were transduced with the respective concentrated virus particles using 10 µg/mL polybrene (Sigma-Aldrich ...
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No products found
because this supplier's products are not listed.
Dongying Chen, et al.,
bioRxiv - Developmental Biology 2024
Quote:
... To harvest protein lysate from IBIDI µSlide ...
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Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and...
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Svenja Fritzlar, et al.,
bioRxiv - Microbiology 2023
Quote:
... virus was activated by adding 8μg/mL trypsin (TPCK treated, Worthington, Lakewood, NJ) in FCS-free media to samples in a 1:1 ratio ...
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No products found
because this supplier's products are not listed.
S. Chul Kwon, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... lysates were incubated with Strep-Tactin beads (IBA Lifesciences) at 4°C overnight ...
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No products found
because this supplier's products are not listed.
Sylvia Varland, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... Cell lysates were mixed with Laemmli sample buffer (Alfa Aesar) and denatured at 95°C for 5 min ...
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No products found
because this supplier's products are not listed.
John K. Mich, et al.,
bioRxiv - Neuroscience 2023
Quote:
Virus injection and optic fiber implantation surgery was performed in C57BL/6J mice (The Jackson Laboratory, #000664) at around P60 ...
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No products found
because this supplier's products are not listed.
Shufeng Liu, et al.,
bioRxiv - Microbiology 2021
Quote:
... Lysates were loaded onto 4-20% SDSPAGE gels and transferred to a nitrocellulose membrane (LI-COR, Lincoln, NE), blocked with Intercept (TBS ...
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No products found
because this supplier's products are not listed.
Meagan N. Esbin, et al.,
bioRxiv - Cell Biology 2024
Quote:
... Precleared lysates were then immunoprecipitated overnight with 4 mg of normal anti-mouse IgG (Jackson ImmunoResearch, #211-032-171) and anti-FLAG [M2] (Sigma Aldrich ...
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No products found
because this supplier's products are not listed.
K Pilarzyk, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Lysates for all WTs within a cohort (Cohort 1: 2F + 3M; Cohort 2: 3M; Cohort 3: 4F; Cohort 4: 6M), and all KOs within a cohort were pooled (Cohort 1 ...
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No products found
because this supplier's products are not listed.
Prashant Rajbhandari, et al.,
bioRxiv - Cell Biology 2019
Quote:
Whole cell lysate or tissue lysate was extracted using RIPA lysis buffer (Boston Bioproducts) supplemented with complete protease inhibitor cocktail (Roche) ...
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No products found
because this supplier's products are not listed.
Doowon Huh, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... Lysate was digested with RNase I (Lucigen) before ribosomes were isolated through ultracentrifugation through a sucrose cushion ...
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No products found
because this supplier's products are not listed.
Timothy P. Sheahan, et al.,
bioRxiv - Microbiology 2020
Quote:
... nanoluciferase expression as a surrogate for virus replication was quantitated on a Spectramax (Molecular Devices) plate reader according to the manufacturer’s instructions (Promega ...
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No products found
because this supplier's products are not listed.
Leena Hussein Bajrai, et al.,
bioRxiv - Microbiology 2021
Quote:
... the plates were analyzed by observing virus-induced CPE by light microscope (Nikon-ECLIPSE-Ti), and plaque formation was determined by crystal violet staining (C0775 ...
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No products found
because this supplier's products are not listed.
Heejoo Kim, et al.,
bioRxiv - Immunology 2020
Quote:
... Lysates were incubated with 2.5 μg Jmjd1A antibody (Bethyl) and protein-G Dynabeads (ThermoFisher ...
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No products found
because this supplier's products are not listed.
Eugenia Zah, et al.,
bioRxiv - Bioengineering 2020
Quote:
... The lysate supernatant was ultracentrifuged with iodixanol (OptiPrep; StemCell Technologies) density gradient solutions (54% ...
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No products found
because this supplier's products are not listed.
Pin-Ji Lei, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... we inoculated 1×105 PFU UV-inactivated PR8 influenza virus + 2μg Anti-CD40 (BioXCell, Cat: BE0016-2) + 2μg poly I:C (InvivoGen ...
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No products found
because this supplier's products are not listed.
Christopher M. Schafer, et al.,
bioRxiv - Cell Biology 2023
Quote:
... Incubation of cell lysates with polyubiquitin-affinity beads (Calbiochem: #662200-1KIT) was carried out according to the manufacturer’s protocol followed by immunoblotting with an anti-ERG Ab (Santa Cruz ...
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No products found
because this supplier's products are not listed.
JF Sturgill, et al.,
bioRxiv - Neuroscience 2020
Quote:
... AAV virus (300nL volume) was then pressure injected 100nL/min via a glass pipette pulled (P-97 Sutter Instruments) from borosilicate capillaries (Drummond calibrated 5ul ...
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No products found
because this supplier's products are not listed.
Thomas H Mahood, et al.,
bioRxiv - Systems Biology 2020
Quote:
... CA) while tissue lysate samples were loaded onto a separate C18 column (Phenomenex) offline ...
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No products found
because this supplier's products are not listed.
Antje Neeb, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... A further lysate (600 μl) was incubated with 5 μg rabbit immunoglobulins (Vector Laboratories) to control for nonspecific interactions ...
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No products found
because this supplier's products are not listed.
Maryann P. Platt, et al.,
bioRxiv - Microbiology 2022
Quote:
... anti-Spn serotype 4 (#16747, Statens Serum Institut) and cleaved-caspase-3 (AF835SP ...
-
No products found
because this supplier's products are not listed.
Soraya H. Foutouhi, et al.,
bioRxiv - Microbiology 2019
Quote:
... serotype Saint Paul (BCW_88) and serotype Newport (BCW_1378) were grown in Luria-Bertani (LB) broth (Teknova, Holister, CA) and incubated with shaking (200 rpm ...
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No products found
because this supplier's products are not listed.
Xiaonan Zhang, et al.,
bioRxiv - Microbiology 2020
Quote:
... The blood samples from enrolled patients were tested for hepatitis B virus surface antigen (HBsAg) and hepatitis B virus e antigen (HBeAg) by Abbott AXSYM HBsAg (normal ...
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No products found
because this supplier's products are not listed.
Emily A. Hemann, et al.,
bioRxiv - Immunology 2022
Quote:
... this virus/serum mixture is incubated with 0.5% chicken red blood cells (Rockland) and hemagglutination inhibition was measured after 30 minutes.
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No products found
because this supplier's products are not listed.
Kim Reid, et al.,
bioRxiv - Cell Biology 2022
Quote:
... The solution was centrifuged 16,000g for 10 minutes at 4°C and the supernatant containing the mitochondrial or cell lysate proteins quantified (Bio-Rad DC Protein Assay, BMG Labtech POLARstar Omega plate reader).
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No products found
because this supplier's products are not listed.
Paula Lagan, et al.,
bioRxiv - Microbiology 2023
Quote:
... Successful virus isolation was confirmed either by haemagglutination of 0.5% chicken blood cells (Envigo #S.B-0008) or by RRT-PCR.
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No products found
because this supplier's products are not listed.
Lavinia Gambelli, et al.,
bioRxiv - Microbiology 2019
Quote:
... Data were collected using a 4 × 4 k CCD camera or a 4×4 k K2 Summit direct electron detector (Gatan Inc., Pleasanton, USA) running in counting mode ...
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No products found
because this supplier's products are not listed.
Miroslav Homola, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... The purified virus was applied onto glow-discharged electron microscopy grids covered with holey carbon (Quantifoil, SPT Labtech, Melbourn, UK), blotted ...
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No products found
because this supplier's products are not listed.
Li Zhenyu, Li Tian, Liu Meisui, Ivanovic Tijana,
bioRxiv - Microbiology 2022
Quote:
... Liposomes consisted of 4:4:2:0.1:2 × 10−4 ratio of 1,2,dioleoyl-sn-glycero-3-phosphocholine (Avanti Polar Lipids), 1-oleoyl-2-palmitoyl-sn-glycero-3-phosphocholine (Avanti Polar Lipids) ...
-
No products found
because this supplier's products are not listed.
Yilin Wang, et al.,
bioRxiv - Immunology 2021
Quote:
... cell lysate (125 μg) was added to 5 μg GGA3-PBD beads (Cytoskeleton, USA) to pull down ARFs-GTP and incubated at 4°C on a rotator for 1h and then the beads were extensively washed to remove unbound protein ...
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No products found
because this supplier's products are not listed.
Nicholas J. Gunn, et al.,
bioRxiv - Microbiology 2023
Quote:
... the lysate was treated with the photoreactive DNA-binding dye PMAxx (Biotium, CA, USA). Briefly ...
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No products found
because this supplier's products are not listed.
Julie M. Button, Suchetana Mukhopadhyay,
bioRxiv - Microbiology 2021
Quote:
Five microliters of purified virus or pelleted cores were placed on a Formvar and carboncoated 300 mesh grid (Ted Pella Inc., Redding, CA) and stained with 1% uranyl acetate ...