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Citations for Cytoskeleton :
1 - 31 of 31 citations for Dengue Virus serotype 4 lysate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... cell lysate (125 μg) was added to 5 μg GGA3-PBD beads (Cytoskeleton, USA) to pull down ARFs-GTP and incubated at 4°C on a rotator for 1h and then the beads were extensively washed to remove unbound protein ...
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bioRxiv - Biophysics 2023Quote: ... and ∼4% rhodamine-tubulin (Cytoskeleton) were incubated with 1 mM GMPCPP or 1 mM GTP with 10 mM taxol at 37°C for 3 h ...
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bioRxiv - Neuroscience 2023Quote: ... Cleared lysates were incubated with Rhotekin-Rho binding domain (RBD) beads (60 µg; Cytoskeleton Inc.) or with PAK-p21 binding domain (PBD ...
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bioRxiv - Cell Biology 2023Quote: ... Cell lysates were then used for G-LISA small GTPase activation assay (Cytoskeleton cat# BK135), where levels of activated RhoA ...
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bioRxiv - Cell Biology 2022Quote: ... Cell lysates were harvested as outlined in the G-lisa RhoA Activation Assay Kit (Cytoskeleton Inc) using the supplied lysis buffer ...
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bioRxiv - Biochemistry 2024Quote: ... The cells were lysed and protein lysates were used for G-LISA analysis per manufacturer’s (Cytoskeleton) instructions ...
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bioRxiv - Microbiology 2024Quote: ... 4 μg of biotinylated tubulin (Cytoskeleton) and 100 μg of unlabelled pig tubulin in BRB80 buffer (80 mM PIPES pH 6.8 ...
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bioRxiv - Cell Biology 2023Quote: ... Protein concentration of the cleared lysates was determined by Precision Red Advanced Protein Assay Reagent (Cytoskeleton Inc.). Equal amounts of total cell lysates were loaded to 4–12% Bis-Tris or 3–8% Tris-Acetate gels (Invitrogen ...
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bioRxiv - Physiology 2021Quote: ... podocytes were fixed in 4% paraformaldehyde and 4% sucrose and stained with Acti-stain™ 488 Phalloidin (Cytoskeleton) and 50 cells per condition were assessed ...
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bioRxiv - Cell Biology 2021Quote: ... cells lysates were prepared according to manufacturer’s instructions (RhoA Pull-Down Activation Assay Biochem Kit; Cytoskeleton Inc., BK036). Specifically ...
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bioRxiv - Cancer Biology 2021Quote: RHOA activity was measured on LN-F lysates using the RHOA G-LISA Activation Assay kit (#BK124, Cytoskeleton). Data are the mean ± SEM of arbitrary units of RHOA activity.
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bioRxiv - Cell Biology 2021Quote: ... Lysates were spun at maximum speed for 10mins before protein level was measured using Precision Red (Cytoskeleton, Inc., #ADV02). Samples were run using precast 4-12% NuPAGE Bis-Tris Acrylamide Gels (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... GTP-bound ARF6 was detected in cell lysates using the ARF6 Activation Assay Biochem Kit™ (Cytoskeleton, BK-033) according to manufacturer’s instructions.
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bioRxiv - Biophysics 2023Quote: ... or α4A/β2A tubulins ∼4% biotin-tubulin (Cytoskeleton) and ∼4% rhodamine-tubulin (Cytoskeleton ...
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bioRxiv - Cell Biology 2022Quote: ... Equal volumes of G-actin and F-actin lysates were analysed by SDS-PAGE and immunoblotting with anti-actin rabbit polyclonal antibody (Cat# AANO1, Cytoskeleton). Densitometric analyses of the G-actin and F-actin levels were performed using ImageJ software ...
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bioRxiv - Neuroscience 2023Quote: ... The pull-down protein samples were then subjected to western blotting with the whole cell lysate and His-tagged Rac1 protein (#RC01, Cytoskeleton) serving as the controls.
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bioRxiv - Molecular Biology 2023Quote: ... SiR-Tubulin incubation was performed for 4 h (Cytoskeleton, with 2 μM SiR-Tubulin and 10 μM verapamil) ...
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bioRxiv - Neuroscience 2023Quote: F- and G-actin fractions from lysates were prepared using a G-Actin/F-Actin in Vivo Assay Biochem kit (Cytoskeleton, #BK037) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: Western blots to determine the ratio of G-actin to F-actin were performed on retinal lysates that were processed using a G-actin/F-actin In Vivo Assay Kit (Cytoskeleton, Inc.) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: Active GTP-bound GTPase levels in brain lysates were measured using G-LISA activation assay kits for Rac1 (Cytoskeleton, Inc., BK128) and RhoA (Cytoskeleton ...
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bioRxiv - Biochemistry 2024Quote: ... Normalized Lysates were incubated with 20μL of anti-FLAG affinity gel or anti-Acetyl-Lysine (mAb mix) Affinity Beads (Cytoskeleton, Inc., AAC04-beads) at 4°C for 2 h ...
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bioRxiv - Neuroscience 2023Quote: ... neurons were fixed in PFA (4% PFA in Cytoskeleton Buffer (CB) – in mM ...
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bioRxiv - Microbiology 2024Quote: Microtubules were made by mixing 4 μg of HiLyte Fluor 488 tubulin (Cytoskeleton), 4 μg of biotinylated tubulin (Cytoskeleton ...
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Importin α/β Promote Kif18B Microtubule Association to Spatially Control Microtubule DestabilizationbioRxiv - Cell Biology 2022Quote: MTs were polymerized by incubating 5 µM tubulin (4% HyLite 647 tubulin (Cytoskeleton #TL670M), ∼1% biotin tubulin (Cytoskeleton #T333P) ...
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bioRxiv - Cell Biology 2020Quote: In order to constitutively activate RhoA vessels at day 4 were perfused with Rho Activator II (Cytoskeleton) at a concentration of 2 µg/mL for four hours prior to use in permeability tests or fixation for immunocytochemistry ...
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bioRxiv - Microbiology 2020Quote: ... Samples were fixed in 4% (v/v) Paraformaldehyde Aqueous Solution (15710-s, EM Grade) in PEM (Cytoskeleton-preserving buffer PIPES-EGTA-Magnesium ...
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bioRxiv - Neuroscience 2020Quote: ... and secondary antibody at 1 h (4 °C) facilitated by an automated western blot liquid handler (Cytoskeleton GOBlot). Antibodies utilised included anti-eIF2α (abcam ...
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bioRxiv - Developmental Biology 2022Quote: ... Protein lysates were centrifugated for 15 min at > 15000 rpm at 4°C and supernatant was collected and quantified by Precision Red Advanced Protein Assay (Cytoskeleton). 5-10 μg of protein were boiled in NuPAGE LDS sample buffer (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... actomyosin contractility of MECs stably expressing an inducible ROCK construct was induced overnight using 4-hydroxytamoxifen and the actin cortex of these cells was labelled with SiR-actin dye (Cytoskeleton) according to manufacturer’s instructions prior to laser ablation.
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bioRxiv - Developmental Biology 2023Quote: ... Supernatant was collected upon 15min of centrifugation at >15000 rpm at 4°C and quantified by Precision Red Advanced Protein Assay (Cytoskeleton). Equal amounts of protein were boiled in NuPAGE LDS sample buffer (Invitrogen ...
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Importin α/β Promote Kif18B Microtubule Association to Spatially Control Microtubule DestabilizationbioRxiv - Cell Biology 2022Quote: Dynamic MT seed assays were performed by polymerizing GMPCPP stabilized Cy5-MT seeds from 4 µM HiLyte 647 tubulin (Cytoskeleton, #TL670M) in BD buffer + 0.5 mM GMPCPP at 37°C for 1 hour ...