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No products found
because this supplier's products are not listed.
Ashley M Ngo, et al.,
bioRxiv - Microbiology 2019
Quote:
... Dengue virus Type 2 NS5 protein (Genetex, GTX629447) and GFP (Genetex ...
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No products found
because this supplier's products are not listed.
Michele Martins, et al.,
bioRxiv - Biochemistry 2020
Quote:
... it was screened for Dengue virus (DENV) using the Dengue NS1 Ag STRIP (Bio-Rad). The use of human blood was approved by the Fiocruz Ethical Committee (CAAE 53419815.9.0000.5248).
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No products found
because this supplier's products are not listed.
Ann Logan, et al.,
bioRxiv - Immunology 2022
Quote:
... after which cells were permeabilised (0.1% Triton X100) and stained with an antibody recognising dengue virus envelope protein (Invitrogen MA1-27093, for DENV serotypes 1-3), pan flavivirus envelope (Millipore MAB10216 ...
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No products found
because this supplier's products are not listed.
Qinrui Wang, et al.,
bioRxiv - Biophysics 2019
Quote:
... 1 L of Sf9 insect cells in Insect-XPRESS Protein-free Insect Cell Medium with L-glutamine (Lonza) in a 3 L flask was infected with 5 ml of the harvested P2 (second passage ...
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No products found
because this supplier's products are not listed.
Maria Vittoria Mancini, et al.,
bioRxiv - Microbiology 2020
Quote:
... Primary antibody for DENV was MAB8705 Anti-dengue virus complex antibody (Millipore); secondary antibody was the Goat anti-mouse Alexa Fluor 488 ...
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No products found
because this supplier's products are not listed.
Hanbo Guo, et al.,
bioRxiv - Biochemistry 2023
Quote:
... The protein was expressed in the Sf9 insect cell (Expression system) in the ESF921 medium using the Bac-to-Bac baculovirus system ...
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No products found
because this supplier's products are not listed.
Shashank Manohar Nambiar, et al.,
bioRxiv - Physiology 2022
Quote:
... Maternal hepatocyte-specific YAP1 deletion was achieved by injecting mice with the adeno-associated virus serotype 8 (AAV8) with the thyroxine-binding globulin promoter (TBG) promoter expressing Cre (AAV8-TBG-Cre virus, Addgene, AV-8-PV1091) via tail vein at a dose of 1×1012 genomic copies/mouse ...
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No products found
because this supplier's products are not listed.
Minyue Qiu, et al.,
bioRxiv - Microbiology 2022
Quote:
... sections were incubated with anti-dengue virus 1+2+3+4 primary antibodies (ab26837, Abcam, England) at 4°C overnight ...
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No products found
because this supplier's products are not listed.
Jonas N. Conde, et al.,
bioRxiv - Microbiology 2023
Quote:
... and recLI9-NS1-GFP11 reporter virus was purified using RNeasy kit (Qiagen). cDNA synthesis was performed as above using random hexamer primers (25°C for 10 min ...
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No products found
because this supplier's products are not listed.
Johanna S. Jackson, et al.,
bioRxiv - Neuroscience 2020
Quote:
... an adeno-associated virus (AAV) serotype 2 expressing GFP (1010 GU; Vector Biolabs) was injected into layer 2/3 (300µm below the dura ...
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No products found
because this supplier's products are not listed.
Jasmine Moshiri, et al.,
bioRxiv - Microbiology 2020
Quote:
... dengue protein NS2B/3/4A constructs were expressed in rabbit reticulocyte lysate (TNT coupled T7, Promega Bio Systems) programmed with 1 µg DNA/50 µL and labeled with 20 µCi of L-[35S]-methionine (EasyTag ...
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No products found
because this supplier's products are not listed.
Romel Rosales Ramirez, Juan E. Ludert,
bioRxiv - Molecular Biology 2019
Quote:
The NS1 sequence from ZIKV (Mexican isolate, Asiatic linkage) and YFV NS1 (Brazilian yellow fever virus isolate) were synthesized de novo (GenScript, Piscataway, NJ). The synthetic ZIKV NS1 gene (GenBank accession number KY631493.1 ...
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No products found
because this supplier's products are not listed.
Wei Zhang, et al.,
bioRxiv - Neuroscience 2023
Quote:
... Adeno-associated virus (AAV) cell-lysates were produced using the AAVpro Purification Kit (All Serotypes) (Takara) with slight modifications ...
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No products found
because this supplier's products are not listed.
Sophie M. Jegouic, et al.,
bioRxiv - Microbiology 2020
Quote:
Infected insect cells were disrupted with CytoBuster™ protein extraction reagent (Merck) and clarified by centrifugation at 4,300 × g for 20m before column loading ...
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No products found
because this supplier's products are not listed.
S.A. Ford, et al.,
bioRxiv - Evolutionary Biology 2019
Quote:
Dengue virus was quantified via RT-qPCR using the LightCycler 480 (Roche). We used the TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher Scientific ...
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No products found
because this supplier's products are not listed.
Yu Cao, et al.,
bioRxiv - Plant Biology 2023
Quote:
... coli or insect cells as fusion proteins subcloned into pGEX-6P-1(GE Healthcare) or te pFastBac-1 vector (Invitrogen) ...
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No products found
because this supplier's products are not listed.
Daniel Perez-Zsolt, et al.,
bioRxiv - Microbiology 2021
Quote:
... Virus particles were immunostained with rabbit anti-SARS-CoV-2 N protein (Sino Biological) or rabbit anti-GM1 Ab (Abcam ...
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No products found
because this supplier's products are not listed.
Marco T. Radukic, et al.,
bioRxiv - Genomics 2020
Quote:
... a plasmid encoding the replicases of AAV serotype 2 and the capsid of AAV serotype 9) and pHelper (Agilent Technologies, lab reference pZMB0088) which provides AAV adenoviral helper functions ...
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No products found
because this supplier's products are not listed.
Saurabh Srivastava, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... Recombinant baculoviruses were recovered by transfection of the bacmids into Sf9 insect cells using TransIt-Insect reagent (Mirus). Viruses were then used to infect suspension High Five cells grown in Express5 media and supernatants harvested ∼60–72 hours post-infection ...
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No products found
because this supplier's products are not listed.
Anne Weiß, Ivan Haralampiev, Susann Kummer,
bioRxiv - Microbiology 2022
Quote:
... anti-NS1 (Santa Cruz Biotechnology), and anti-beta-actin (Sigma Aldrich ...
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No products found
because this supplier's products are not listed.
Elaine Cristina Matos Vicentin, et al.,
bioRxiv - Immunology 2020
Quote:
... of 5 μg of each mAb (αDEC-NS1, αDCIR2-NS1, αDEC or αDCIR2) together with 50 μg poly (I:C) (Invivogen), exactly as described in (17) ...
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No products found
because this supplier's products are not listed.
Charles-Francois V. Latchoumane, et al.,
bioRxiv - Neuroscience 2023
Quote:
... Viruses were produced with Serotype 1 or DJ (Cell Biolabs, Inc.) and purified by CsCl gradients 67 ...
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No products found
because this supplier's products are not listed.
Sanchita Mishra, et al.,
bioRxiv - Immunology 2024
Quote:
... anti-SARS-CoV-2 N protein (Cell Signaling Technology, 33717). HRP-conjugated secondary antibodies used in this study were Jackson Immuno Research Laboratories anti-rabbit (111-035-047 ...
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No products found
because this supplier's products are not listed.
Bolutife Fakoya, et al.,
bioRxiv - Microbiology 2021
Quote:
... cholerae serotype specific antisera (BD Difco) was performed using glass slides to confirm serotypes ...
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No products found
because this supplier's products are not listed.
Pei Xuan Lee, et al.,
bioRxiv - Microbiology 2020
Quote:
... Purified NS1 protein from D2Y98P or 16681 (10 ng/well) diluted in PBS was coated onto 96-well EIA plates (Corning costar) overnight at 4 °C ...
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No products found
because this supplier's products are not listed.
Komal Raj Rijal, et al.,
bioRxiv - Microbiology 2020
Quote:
... The data presented in this study represents serological diagnosis using rapid test kit (SD Bioline dengue IgG/IgM antibody up to 2015; and after 2015, SD Bioline dengue duo (dengue NS1 Ag+ IgG/IgM), Korea ...
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No products found
because this supplier's products are not listed.
Laurie-Anne Lamotte, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... together with Anti-Influenza A Non-Structural Protein 1 (NS1) Mouse Monoclonal Antibody (EMB005, Kerafast). Cells were then washed and incubated with a mixture of secondary antibodies ...
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No products found
because this supplier's products are not listed.
Marco Thürkauf, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... The AAV transfer vectors used for 3-plex sgRNA delivery into skeletal muscle were cloned between AAV serotype 2 ITR’s including a cloning site for multiplexed hU6-sgRNA insertions (MluI and KpnI (NEB)) ...
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No products found
because this supplier's products are not listed.
Ferdos Abid Ali, et al.,
bioRxiv - Cell Biology 2023
Quote:
Sf9 insect cells (VWR, #EM71104-3) were maintained in ExCell 420 serum-free media (Sigma ...
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No products found
because this supplier's products are not listed.
Martin Müller, et al.,
bioRxiv - Microbiology 2023
Quote:
... SARS-CoV-2 ΔORF6-YFP and SARS-CoV-2 ΔORF6-YFP ΔORF3c infected CaCo-2 wild-type cells and (2) transfected HEK293T cells infected with Sendai virus (Cantell Strain) (Charles River, l0l00774) for 8 hours ...
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No products found
because this supplier's products are not listed.
Conor J. Crawford, et al.,
bioRxiv - Microbiology 2019
Quote:
... neoformans Serotype A strain H99 (American Type Culture Collection (ATCC) 208821) ...
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No products found
because this supplier's products are not listed.
Yidong Wang, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... ZR Tissue & Insect RNA MicroPrep™ (Zymo Research Corp. ...
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No products found
because this supplier's products are not listed.
Rajeev Rudraraju, et al.,
bioRxiv - Microbiology 2022
Quote:
Cytokine/chemokine concentrations in supernatant from mock and SARS- CoV-2 infected lung AT2 and cardiac cells were analyzed using the LEGENDplex human anti-virus response panel (Biolegend, Cat. 740390) following the manufacturer’s instructions ...
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No products found
because this supplier's products are not listed.
Kristina Lanko, et al.,
bioRxiv - Microbiology 2020
Quote:
Viral RNA was isolated from virus stocks with Nucleospin RNA virus (Macherey-Nagel) according to manufacturer protocol ...
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No products found
because this supplier's products are not listed.
Amish N. Raval, et al.,
bioRxiv - Pathology 2024
Quote:
... Cell surface protein expression was performed by labeling 2×105 cells for KDR-PE (R&D Systems, FAB357P) + PDGFRα-APC (Cell Signaling ...
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No products found
because this supplier's products are not listed.
Vincent Basse, et al.,
bioRxiv - Microbiology 2021
Quote:
... Purified NS1 was then concentrated using Vivaspin columns (Sartorius).
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No products found
because this supplier's products are not listed.
A.A. Shaldzhyan, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... one milliliter of NS1 recombinant protein (3 mg/ml in 2x PBS) was placed into a 1.5 ml Eppendorf tube (Eppendorf, Germany); and the tube mixed for 66 hours (55°C ...
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No products found
because this supplier's products are not listed.
Paulo V. M. Boratto, et al.,
bioRxiv - Microbiology 2020
Quote:
... the μl of virus solution were placed on glow discharged C-Flat 2/2 grids (EMS) and plunge frozen into liquid ethane using a Gatan Cryoplunge 3 ...
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No products found
because this supplier's products are not listed.
Daryl M. Gohl, et al.,
bioRxiv - Genomics 2020
Quote:
... with the respiratory virus oligo panel including SARS-CoV-2 probes (Illumina, San Diego ...
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No products found
because this supplier's products are not listed.
Guillem Torcal Garcia, et al.,
bioRxiv - Cell Biology 2022
Quote:
... the virus-cell mixture was centrifuged at 1,000 rcf for 2 hours at 32°C (Beckman Coulter, Allegra X-30R). Infected cells were then cultured as described above and subsequently FACS-sorted for the establishment of clonal cell lines.
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No products found
because this supplier's products are not listed.
Monika Moissidis, et al.,
bioRxiv - Neuroscience 2024
Quote:
Serotype 8 adeno-associated viruses (AAV8) were produced in HEK293FT cells grown on 5 (Polysciences Europe, 23966-100) or 10 15-cm diameter plates (Sigma-Aldrich #408727) ...
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No products found
because this supplier's products are not listed.
Jonathan Leis, et al.,
bioRxiv - Microbiology 2020
Quote:
... Virus released from cells was quantified by media-associated p24 determined by ELISA (PerkinElmer) and equivalent amounts of p24.
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No products found
because this supplier's products are not listed.
Donghwi Bae, et al.,
bioRxiv - Cell Biology 2022
Quote:
... 2% SM1 (Stem Cell Technologies), 15mM HEPES (Gibco) ...
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Recombinant Antigen
Cat# REC31779-100,
100µg USD $282.0
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Richard S Tedder, et al.,
bioRxiv - Microbiology 2019
Quote:
Recombinant DV NS1 antigens (rDVNS1Ag) from the four serotypes (DV 1-4 inclusive) expressed in mammalian cells (The Native Antigen Company, Kidlington, Oxfordshire, OX5 1LH, UK) were used in molar excess as components of the conjugate diluent.
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No products found
because this supplier's products are not listed.
Max Paget, et al.,
bioRxiv - Immunology 2022
Quote:
... MDCK cells expressing IAV-NS1 (MDCK-NS1 cells) were infected with IAVdelNS1 in EMEM containing 0.35% bovine serum albumin (BSA, MP Biomedicals), 4 mM L-glutamine ...
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No products found
because this supplier's products are not listed.
Stephen Graham, et al.,
bioRxiv - Immunology 2020
Quote:
... The virus-monoclonal antibody mixture was added (2 mL) to Vero cells in a 6-well plate (Greiner). After infecting the cells for 1 hour at 37°C ...
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No products found
because this supplier's products are not listed.
Kelly Mitchell, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... and virus was concentrated with PEG-it Virus Precipitation solution (System Biosciences). SORE6-GFP virus was added to CSCs plated on Geltrex ...
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No products found
because this supplier's products are not listed.
Sheng Xiao, et al.,
bioRxiv - Neuroscience 2023
Quote:
... AAV virus was then infused via a blunt 36-gauge stainless steel needle (World Precision Instruments NF36BL-2) connected to a microliter injection system (10 µL ...
-
No products found
because this supplier's products are not listed.
Yubao Fan, et al.,
bioRxiv - Cell Biology 2022
Quote:
... 10 ng/ml bone morphogenetic protein-2 (BMP2; Peprotech), 1% penicillin-streptomycin solution (Thermo Fisher Scientific ...
-
No products found
because this supplier's products are not listed.
M. Verónica López, et al.,
bioRxiv - Immunology 2021
Quote:
... The cells were incubated for 18 h at 37 °C at 1.5×106 cells per well with 2 μg/ml of SARS-CoV-2 Spike protein peptide pool (PepTivator® SARS-CoV-2 Prot_S, Miltenyi Biotec, Germany). Cells were stained with anti-CD8α (APC) ...