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Citations for Sartorius :
1 - 50 of 679 citations for Dengue Virus Serotype 2 NS1 Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Purified NS1 was then concentrated using Vivaspin columns (Sartorius).
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bioRxiv - Molecular Biology 2019Quote: Live cells monitoring of virus infection was performed using the IncuCyte Live-Cell Analysis system (Sartorius). Cells were seeded in a 24-well plated at 80% confluency ...
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bioRxiv - Immunology 2023Quote: ... pseudotyped SARS-CoV-2 virus particles were harvested and filtered over a 0.45 µm nitrocellulose membrane (Sartorius Stedim, Gottingen, Germany). SARS-CoV-2 pseudovirus productions were quantified by RETRO-TEK HIV-1 p24 ELISA according to manufacturer instructions (ZeptoMetrix Corporation).
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bioRxiv - Systems Biology 2020Quote: ... virus was grown on Vero E6 cells and concentrated using Vivaspin® 20 concentrators (Sartorius Stedim Biotech). Virus stocks were stored at −80°C ...
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bioRxiv - Molecular Biology 2020Quote: ... The collected virus was filtered (0.45 μm filter, Sartorius) and concentrated using PEG-it overnight at 4°C (SBI Integrated Sciences ...
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bioRxiv - Microbiology 2022Quote: ... Infectious virus titer was determined using an Incucyte system (Sartorius) to quantify the red fluorescence from the mCherry reporter signal as detailed above ...
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bioRxiv - Microbiology 2021Quote: ... and virus particles were enriched in Vivaspin 20 columns (Sartorius Stedim, Biotech) via centrifugation ...
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bioRxiv - Microbiology 2021Quote: ... the virus supernatant was concentrated using Vivaspin 20 concentrators (Sartorius Stedim Biotech) by spinning at 6000 g for 30 min ...
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bioRxiv - Cancer Biology 2023Quote: ... virus was concentrated using the 100000 MWCO Vivaspin® Concentrator (Sartorius Stedim) and titrated on mammary epithelial cells ...
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bioRxiv - Biophysics 2024Quote: ... His-tagged dROS1 protein (construct 2-3) were loaded onto penta-His sensors (Sartorius) to a response unit of about 0.5 nm followed by quenching in 10 ng/mL biocytin (Sigma-Aldrich ...
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bioRxiv - Immunology 2022Quote: ... Virus was concentrated by centrifugation in a 100,000 MWCO ultrafiltration unit (Vivaspin, Sartorius). Titers of virus stocks were determined by plaque assay on BV2 cells(57).
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bioRxiv - Cell Biology 2019Quote: ... Virus was amplified and purified using AdenoPACK 20 Adenovirus (Ad5) purification & concentration kit (Sartorius).
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bioRxiv - Developmental Biology 2020Quote: ... 10 mL of virus supernatant was filtered through a 0.45 um filter (Sartorius, 16533) and supplemented with 8µg/ml polybrene (Millipore ...
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bioRxiv - Microbiology 2023Quote: ... the virus-containing medium was filtered through a 0.45 µm membrane filter (Sartorius®) after 2.5 days ...
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bioRxiv - Plant Biology 2021Quote: ... Proteins eluting from the column were concentrated by ultrafiltration on Vivaspin 20 and 2 columns (Sartorius) with a 5 kDa molecular weight cut-off ...
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bioRxiv - Microbiology 2021Quote: ... supernatant was harvested and virus particles were purified from cytokines and concentrated using Vivaspin 20 (Sartorius, filtration units with a size exclusion of 100 kDa ...
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bioRxiv - Developmental Biology 2019Quote: ... 10 mL of virus supernatant were harvested by filtering through a 0.45 um filter (Sartorius, 16533) and supplemented with 8μg/ml polybrene (Millpore ...
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bioRxiv - Molecular Biology 2022Quote: ... the virus was purified using an Adenovirus (Ad5) Purification and Concentration Kit (AdenoPACK 20; Sartorius, Goettingen, Germany) according to the manufacturer’s instructions and stored at −80 °C until use ...
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bioRxiv - Immunology 2022Quote: ... 200 nM of randomly biotinylated SARS-CoV-2 S or RBD protein antigen was captured using SA biosensors (18-5019, Sartorius). The biosensor was loaded with antigen for 5 min and then moved into the saturating mAbs at a concentration of 100 µg/mL for 10 min ...
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bioRxiv - Immunology 2020Quote: ... the supernatant containing virus particles was harvested and filtered with a 0.45 μm syringe filter (Sartorius Stedim Biotech). Filtered viral supernatants were concentrated 100 times using Lenti-X™ Concentrator (Takara Bio ...
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bioRxiv - Bioengineering 2023Quote: ... The samples were then passed through 0.22 μm filter followed by 20 nm ViroSart virus filter (Sartorius Stedim, Germany). The clarified samples were then reconstituted in formulation buffer ...
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bioRxiv - Bioengineering 2022Quote: ... Four images were recorded per well every 2 h and analyzed using IncuCyte cell by cell analysis software module (Sartorius). The killing potency of the CAR-T cells was assessed by comparing the percentage of Annexin V positive target cells over time relative to the total number of target cells.
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bioRxiv - Microbiology 2019Quote: ... cells were fixed in 2% paraformaldehyde and GFP positive cells were quantified by flow cytometry (Intellicyt iQue Screener Plus, Sartorius AG). Dose-response neutralization curves were analyzed by non-linear regression with a variable slope (GraphPadPrism v8 ...
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bioRxiv - Microbiology 2023Quote: ... cells were fixed in 2% paraformaldehyde and GFP positive cells were quantified by flow cytometry (Intellicyt iQue Screener Plus, Sartorius AG). For experiments using single dilutions of serum or ExpiCHO-S cell supernatant ...
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bioRxiv - Microbiology 2023Quote: ... Cells were fixed with 2% paraformaldehyde and % GFP positive cells were determined using flow cytometry (Intellicyt iQue Screener PLUS, Sartorius AG). Infection was normalized to conditions without antibody ...
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bioRxiv - Microbiology 2022Quote: The filtration efficiency of AF-Fe against virus-loaded aerosols was assessed using a compact experimental setup composed mainly of a polycarbonate filtration holder (Sartorius, Germany) connected to three 1.5 l compressed air tanks (PanGas ...
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bioRxiv - Microbiology 2022Quote: ... Cells were disrupted by beat beating for 2 minutes using 0.4-0.6 mm glass beads (Sartorius). Beads were pelleted by centrifugation (21,000 × g ...
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bioRxiv - Neuroscience 2023Quote: ... The recovered virus fraction was purified by first passing through a 100-kDA molecular weight cut-off (MWCO) centrifugal filter (Sartorius VIVASPIN VS2041) and then through an Amicon Ultra 2ml Centrifugal filter (Millipore UFC210024) ...
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Somatostatin interneurons control the timing of developmental desynchronization in cortical networksbioRxiv - Neuroscience 2023Quote: ... The recovered virus fraction was purified by first passing through a 100 kDa molecular weight cutoff (MWCO) centrifugal filter (Sartorius, VIVASPIN VS2041) and then through an Amicon Ultra 2 ml Centrifugal filter (Millipore ...
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bioRxiv - Neuroscience 2024Quote: ... The recovered virus fraction was purified by first passing through a 100 kDa molecular weight cutoff (MWCO) centrifugal filter (Sartorius, VIVASPIN VS2041) and then through an Amicon Ultra 2mL Centrifugal filter (Millipore #UFC210024) ...
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bioRxiv - Neuroscience 2021Quote: ... The recovered virus fraction was purified by first passing through a 100-kDa molecular weight cut off (MWCO) centrifugal filter (Sartorius Vivaspin, Cat# VS2041) and then through an Amicon Ultra 2ml Centrifugal filters (Merck Millipore ...
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bioRxiv - Synthetic Biology 2023Quote: ... The cell culture was inoculated at OD600 0.3 in the 2 L bioreactor (Biostat B, Sartorius, Germany) including the 1 L modified M9 minimal medium ...
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bioRxiv - Immunology 2020Quote: ... GFP fluorescent intensity was detected every 2 hours using the IncuCyte S3 Live-Cell analysis system (Sartorius, Germany). Total integrated GFP intensity per well was used as a quantitative measure of viable target cells ...
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bioRxiv - Cancer Biology 2023Quote: ... Parental and MCT1-KO MIA PaCa-2 cells were transduced with Lentiviral NucLight Red and Green vectors (Sartorius), respectively ...
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bioRxiv - Immunology 2020Quote: ... Protein A biosensors (FortéBio/Sartorius) were first hydrated for 10 minutes in Kinetics buffer ...
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bioRxiv - Biochemistry 2023Quote: ... each Protein A biosensors (Sartorius) was pre-equilibrated in PBST buffer (PBS ...
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bioRxiv - Systems Biology 2022Quote: ... live-cell proliferation and expression of YFP-tagged proteins were tracked for 48 h using an Incucyte S3 device (Sartorius).
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bioRxiv - Genomics 2023Quote: ... 2% Ultroser-G (Sartorius Stedim Biotech GmbH ...
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bioRxiv - Systems Biology 2023Quote: ... The wells were imaged every 2 hours for 120 hours and cell confluence was measured with Incucyte ZOOM (Sartorius).
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bioRxiv - Cancer Biology 2024Quote: ... Glioma cells were stably transduced with a lentiviral nuclear red fluorescent protein under the EF1a promotor (Sartorius, cat. 4476, Göttingen, Germany) for visualization in live imaging assays ...
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bioRxiv - Immunology 2022Quote: ... Protein A biosensors (Sartorius, #18-5010) were hydrated in BLI assay buffer for 10-20 minutes and loaded onto an Octet Red96 BLI instrument (Pall ...
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bioRxiv - Cell Biology 2021Quote: ... Brightfield images were taken every 2 hours for 5 days and the percentage confluency calculated using an Incucyte Live-Cell analysis system (Sartorius).
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Images and confluence data were acquired every 2 h for ~200 h using the IncuCyte Live Cell Analysis Imaging System (Sartorius). The Area Under Curve (AUC ...
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bioRxiv - Physiology 2024Quote: ... Cultures were monitored for colony formation from 2 to 8 weeks after plating using Incucyte live cell imaging system (Sartorius). Wells with single colonies were harvested and cell cultures were expanded for making stocks and for DNA isolation ...
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bioRxiv - Microbiology 2019Quote: ... Residual proteins were then eliminated by filtration on 0.22-µm high-protein-retention Ministart syringe filters (Sartorius Stedim Biotech GmbH ...
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bioRxiv - Neuroscience 2024Quote: ... 2% Ultroser G (Pall-Sartorius) and 2% Penicillin-Streptomycin (Life technologies) ...
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bioRxiv - Molecular Biology 2023Quote: ... The cells were resuspended in 2 mL 50 mM Tris buffer (pH 7.6) and lysed by using a dismembrator (Sartorius, Göttingen, Germany) at 1,600 rpm for 3 min ...
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bioRxiv - Microbiology 2020Quote: ... For all recombinant proteins,protein-containing fractions were pooled and buffer exchange to PBS via VivaSpin columns (Sartorius) was performed ...
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bioRxiv - Neuroscience 2020Quote: ... using protein concentrator tubes (VIVASPIN 20, Sartorius). Both purified IgG fractions from patient and controls were re-tested on CBAs and the anti-IgLON5 IgG concentration was determined (titer 1:100) ...
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bioRxiv - Cancer Biology 2020Quote: Proteins were transferred to nitrocellulose membrane (Sartorius). The membranes were blocked with 10% BSA (Santa Cruz Biotechnology ...