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Dengue Virus Serotype 2 NS1 Protein HEK293
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Genetex
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2019, published in eLife doi: 10.7554/eLife.48469Quote: ... Dengue virus Type 2 NS5 protein (Genetex, GTX629447) and GFP (Genetex ... -
Bio-Rad
No products found because this supplier's products are not listed.bioRxiv - Biochemistry 2020Quote: ... it was screened for Dengue virus (DENV) using the Dengue NS1 Ag STRIP (Bio-Rad). The use of human blood was approved by the Fiocruz Ethical Committee (CAAE 53419815.9.0000.5248). -
Millipore Sigma
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2020Quote: ... Primary antibody for DENV was MAB8705 Anti-dengue virus complex antibody (Millipore); secondary antibody was the Goat anti-mouse Alexa Fluor 488 ... -
Thermo Fisher
No products found because this supplier's products are not listed.bioRxiv - Immunology 2021Quote: ... were coated overnight with either Dengue 2 fixed virus antigen (Microbix, Cat# EL-22-02) or polyvalent total immunoglobulin (Invitrogen, Cat# H17000). Before adding PBMC’s ... -
Addgene
No products found because this supplier's products are not listed.bioRxiv - Neuroscience 2021Quote: ... Circuit-specific viral expression was achieved using the retrograde properties of adeno-associated virus (AAV) serotype 5: AAV5.hSyn.HI.eGFP-Cre.WPRE.SV40 (Addgene; #105540). Sparse labelling was obtained using Cre-inducible ... -
Bioline
No products found because this supplier's products are not listed.bioRxiv - Epidemiology 2018Quote: ... The diagnosis of dengue fever was established by the documentation of dengue NS1 antigen (Bioline Dengue NS1 Ag kit ... -
abcam
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2022Quote: ... sections were incubated with anti-dengue virus 1+2+3+4 primary antibodies (ab26837, Abcam, England) at 4°C overnight ... -
Roche
No products found because this supplier's products are not listed.bioRxiv - Evolutionary Biology 2019, published in Frontiers in Microbiology doi: 10.3389/fmicb.2020.01456Quote: Dengue virus was quantified via RT-qPCR using the LightCycler 480 (Roche). We used the TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher Scientific ... -
Sino Biological
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2021Quote: ... Virus particles were immunostained with rabbit anti-SARS-CoV-2 N protein (Sino Biological) or rabbit anti-GM1 Ab (Abcam ... -
Vector Biolabs
No products found because this supplier's products are not listed.bioRxiv - Neuroscience 2020Quote: ... an adeno-associated virus (AAV) serotype 2 expressing GFP (1010 GU; Vector Biolabs) was injected into layer 2/3 (300µm below the dura ... -
GenScript
No products found because this supplier's products are not listed.Cited in The secretory fate of flavivirus NS1 in mosquito cells is influenced by the caveolin binding domainbioRxiv - Molecular Biology 2019Quote: The NS1 sequence from ZIKV (Mexican isolate, Asiatic linkage) and YFV NS1 (Brazilian yellow fever virus isolate) were synthesized de novo (GenScript, Piscataway, NJ). The synthetic ZIKV NS1 gene (GenBank accession number KY631493.1 ... -
Promega
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2020Quote: ... dengue protein NS2B/3/4A constructs were expressed in rabbit reticulocyte lysate (TNT coupled T7, Promega Bio Systems) programmed with 1 µg DNA/50 µL and labeled with 20 µCi of L-[35S]-methionine (EasyTag ... -
Agilent
No products found because this supplier's products are not listed.bioRxiv - Genomics 2020Quote: ... a plasmid encoding the replicases of AAV serotype 2 and the capsid of AAV serotype 9) and pHelper (Agilent Technologies, lab reference pZMB0088) which provides AAV adenoviral helper functions ... -
Qiagen
No products found because this supplier's products are not listed.bioRxiv - Genetics 2020Quote: ... Total viral RNA was extracted from patient specimens and tested for the presence of SARS-CoV-2 with an EZ1 virus extraction kit and EZ1 Advanced XL instrument with the Virus card (Qiagen), or QIASymphony DSP Virus kit and QIASymphony instrument (Qiagen). -
Takara Bio
No products found because this supplier's products are not listed.bioRxiv - Molecular Biology 2020Quote: ... AAVPro Purification Kit (All Serotypes)(Takara Bio USA, Mountain View, CA, USA) were also used following the 48-72 h incubation period ... -
GE Life Sciences
No products found because this supplier's products are not listed.bioRxiv - Molecular Biology 2020Quote: ... Recombinant NS1-6xHis protein was further purified by gel filtration using an AKTA pure chromatograph (GE Healthcare, USA). An XK 16/60 Chromatographic Column ... -
Corning
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2020Quote: ... Purified NS1 protein from D2Y98P or 16681 (10 ng/well) diluted in PBS was coated onto 96-well EIA plates (Corning costar) overnight at 4 °C ... -
Invivogen
No products found because this supplier's products are not listed.bioRxiv - Immunology 2020Quote: ... of 5 μg of each mAb (αDEC-NS1, αDCIR2-NS1, αDEC or αDCIR2) together with 50 μg poly (I:C) (Invivogen), exactly as described in (17) ... -
Merck
No products found because this supplier's products are not listed.bioRxiv - Neuroscience 2020Quote: ... serotype O111:B4 were purchased from Merck Millipore Ltd ... -
American Type Culture Collection
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2019Quote: ... neoformans Serotype A strain H99 (American Type Culture Collection (ATCC) 208821) ... -
New England Biolabs
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2021Quote: ... The JEV NS1 gene was PCR-amplified with Deep Vent polymerase (New England Biolabs) using forward primer OSV 389 and sequential reverse primers OSV 381 ... -
Becton, Dickinson and Company
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2021Quote: ... cholerae serotype specific antisera (BD Difco) was performed using glass slides to confirm serotypes ... -
Lonza
No products found because this supplier's products are not listed.bioRxiv - Immunology 2020Quote: HEK293 cells (Lonza) were cultured in DMEM (Lonza ... -
Polysciences
No products found because this supplier's products are not listed.Cited in Aquaporin-3 regulates endosome-to-cytosol transfer via lipid peroxidation for cross presentationbioRxiv - Immunology 2020Quote: HEK293 cells stably expressing fluorescently-tagged AQP3 (an AQP3-DsRed2 fusion protein) were incubated with 1 μm latex beads (Polysciences) for 2 hours prior to fixation with 2% paraformaldehyde (PFA)/0.2% glutaraldehyde ... -
Santa Cruz
No products found because this supplier's products are not listed.Cited in BRAWNIN: A sORF-encoded Peptide Essential for Vertebrate Mitochondrial Complex III AssemblybioRxiv - Cell Biology 2020Quote: ... 25% of total crude virus extracted was used to infect U87MG for 2 days before G418 (Santa Cruz) selection at 1.2mg/mL. -
R&D Systems
No products found because this supplier's products are not listed.bioRxiv - Immunology 2019Quote: ... and macrophage inflammatory protein-2 (MIP-2) (R&D Systems, Minneapolis, MN) according to the manufacturer’s instructions ... -
Sartorius
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2021Quote: ... Purified NS1 was then concentrated using Vivaspin columns (Sartorius). -
Eppendorf
No products found because this supplier's products are not listed.bioRxiv - Molecular Biology 2020Quote: ... one milliliter of NS1 recombinant protein (3 mg/ml in 2x PBS) was placed into a 1.5 ml Eppendorf tube (Eppendorf, Germany); and the tube mixed for 66 hours (55°C ... -
PerkinElmer
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2017, published in mBio doi: 10.1128/mBio.00939-17Quote: ... NS1 substrates were labeled with Tran35S label (Perkin Elmer, Waltham, MA) by incubation in methionine- and cysteine free media containing 10% dialyzed FBS for 20 min before the addition of 200 μCi/mL of Tran35S label ... -
Illumina
No products found because this supplier's products are not listed.bioRxiv - Genomics 2020Quote: ... with the respiratory virus oligo panel including SARS-CoV-2 probes (Illumina, San Diego ... -
Mirus Bio
No products found because this supplier's products are not listed.bioRxiv - Systems Biology 2021Quote: ... torque teno virus hypothetical protein or human respirovirus 3 D protein were transfected into the cells using TransIT-LT1 (Mirus Bio) according to manufacturer’s protocol ... -
Beckman
No products found because this supplier's products are not listed.bioRxiv - Bioengineering 2021Quote: ... or using flow cytometry (HEK293, CytoFlex, Beckman Coulter). -
Charles River Labs
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2021Quote: ... Rescued virus was then amplified via inoculation of virus into 10-day-old embryonated chicken eggs (Charles River) at 37 °C for three days. -
Cell Signaling Technology
No products found because this supplier's products are not listed.bioRxiv - Molecular Biology 2020Quote: ... HEK293 lysates were collected using RIPA buffer (Cell Signaling Technology), and the protein concentrations of the lysates were quantified through Bradford assays (Bio-Rad ... -
BioLegend
No products found because this supplier's products are not listed.bioRxiv - Immunology 2020Quote: The serum levels of a total of 12 virus-related cytokines were measured by a bead-based immunoassay panel (Mouse Anti-Virus Panel, Cat No: 740622, Biolegend, USA). The BALF levels of a total of 12 inflammatory cytokines were measured by a bead-based immunoassay panel (Mouse Inflammation Panel ... -
Tib Molbiol
No products found because this supplier's products are not listed.bioRxiv - Epidemiology 2018Quote: ... or dengue virus RNA (TIB Molbiol, Lightmix kit ... -
Electron Microscopy Sciences
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2020Quote: ... the μl of virus solution were placed on glow discharged C-Flat 2/2 grids (EMS) and plunge frozen into liquid ethane using a Gatan Cryoplunge 3 ... -
Peprotech
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2019, published in Stem Cell Reports doi: 10.1016/j.stemcr.2019.12.007Quote: ... 2 ng ml−1 recombinant human TGFβ1 (112 amino acid, HEK293-derived, Peprotech, 100-21). Cells were routinely maintained in E8 medium on 1:800 diluted growth factor reduced Matrigel (see below) ... -
Macherey-Nagel GmbH
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2020Quote: Viral RNA was isolated from virus stocks with Nucleospin RNA virus (Macherey-Nagel) according to manufacturer protocol ... -
System Biosciences
No products found because this supplier's products are not listed.Cited in A rectal cancer model establishes a platform to study individual responses to chemoradiationbioRxiv - Cancer Biology 2019Quote: ... the virus media was concentrated using PEG-it Virus Precipitation Solution (SBI System Biosciences), and then the lentiviral particles resuspended in 300 µL PBS ... -
VWR
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2021Quote: ... P1 virus was propagated to passage 2 (P2) by infecting 50 ml of SF9 (VWR, #EM71104-3) culture and harvesting after 5–7 days as described above ... -
World Precision Instruments
No products found because this supplier's products are not listed.bioRxiv - Neuroscience 2021Quote: ... Approximately 1 µl (2 x 1010 GC/hemisphere) virus was dispensed using a NanoFil syringe with a 33G beveled needle (World Precision Instruments). The other hemisphere was injected in the same way ... -
The Native Antigen Company
Recombinant AntigenCat# DENV2-NS1-100, 100µg USD $274.0 AskbioRxiv - Microbiology 2019, published in PLOS ONE doi: 10.1371/journal.pone.0215708Quote: Recombinant DV NS1 antigens (rDVNS1Ag) from the four serotypes (DV 1-4 inclusive) expressed in mammalian cells (The Native Antigen Company, Kidlington, Oxfordshire, OX5 1LH, UK) were used in molar excess as components of the conjugate diluent. -
Acrobiosystems
No products found because this supplier's products are not listed.bioRxiv - Biochemistry 2020Quote: ... Data analysis was performed using Byonic 2.3 software and manually using Xcalibur 4.2 and GlycoWorkbench 1.1. The N protein expressed in HEK293 cells (Cat. No. NUN-C5227) was purchased from AcroBiosystems (Newark, DE). -
Mabtech
No products found because this supplier's products are not listed.bioRxiv - Immunology 2020Quote: ... The SARS-CoV-2 S2 N defined peptide pool contains 41 synthetic peptides binding to human HLA derived from the S and N proteins of the SARS-CoV-2 virus (#3620-1, Mabtech AB) 20 ... -
BPS Bioscience
No products found because this supplier's products are not listed.bioRxiv - Immunology 2021Quote: ... HEK293 cells expressing human Angiotensin converting enzyme 2 (ACE2) (BPS Biosciences, Cat #79951) were cultured in EMEM containing 10% FBS and 5 mg/mL Puromycin to select for ACE2-expressing cells ... -
Cell Biolabs
No products found because this supplier's products are not listed.bioRxiv - Neuroscience 2020Quote: ... Type 2 serotypes of rAAV vectors were prepared using the AAV Helper Free Packaging System (Cell Biolabs, San Diego, CA, USA). The serotypes were produced in HEK 293 cells using a helper virus-free system and were purified using two CsCl2 density gradients and titrated by quantitative polymerase chain reaction ... -
Greiner
No products found because this supplier's products are not listed.bioRxiv - Immunology 2020Quote: ... The virus-monoclonal antibody mixture was added (2 mL) to Vero cells in a 6-well plate (Greiner). After infecting the cells for 1 hour at 37°C ... -
RayBiotech
No products found because this supplier's products are not listed.bioRxiv - Immunology 2021Quote: ... or SARS-CoV-2 spike protein (RayBiotech) overnight at 4°C ... -
Drummond Scientific
No products found because this supplier's products are not listed.bioRxiv - Neuroscience 2020Quote: ... and virus was injected using either a Nanoject (Drummond Scientific) and glass pulled pipette or a syringe pump (Harvard Scientific ...