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Citations for Thermo Fisher :
1 - 50 of 10000+ citations for Dengue Virus Serotype 2 NS1 Protein HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... after which cells were permeabilised (0.1% Triton X100) and stained with an antibody recognising dengue virus envelope protein (Invitrogen MA1-27093, for DENV serotypes 1-3), pan flavivirus envelope (Millipore MAB10216 ...
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bioRxiv - Immunology 2024Quote: ... Antibodies used: anti-Influenza A virus NS1 (PA5-32243, ThermoFisher), anti-acetyl-α-tubulin (Ly640 ...
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bioRxiv - Microbiology 2024Quote: ... NS1 proteins were detected using DENV2 NS1 polyclonal antibody (1:1,000 ThermoFisher Scientific) with overnight incubation at 4 °C ...
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bioRxiv - Immunology 2021Quote: ... were coated overnight with either Dengue 2 fixed virus antigen (Microbix, Cat# EL-22-02) or polyvalent total immunoglobulin (Invitrogen, Cat# H17000). Before adding PBMC’s ...
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bioRxiv - Microbiology 2020Quote: ... The dengue 2 E protein antibody PA5-32246 was used at 1:10,000 for western blotting (Thermo-Fisher Scientific, Waltham, MA). Secondary detection for western blotting used anti-rabbit HRP antibody diluted 1:10,000 (Amersham ECL ...
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bioRxiv - Microbiology 2023Quote: Plasmid DNA from either wild type dengue plasmid or mutated dengue plasmid were quantified using a High Sensitivity Range Qubit (Thermo Fisher). Library preparation was carried out with the Nextera XT DNA Library Prep kit (Illumina ...
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bioRxiv - Immunology 2021Quote: ... NS1 (Invitrogen Cat #PIPA532243, 1:1000), NP (BioRad Cat#MCA400 ...
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bioRxiv - Neuroscience 2023Quote: ... HEK293 cells were infected with virus particles and selected with hygromycin B (200 µg/ml, Invitrogen). For single clone selection ...
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bioRxiv - Microbiology 2021Quote: ... were transfected with pcineo plasmids (0.4 μg) carrying the RSV codon-optimised NS1 or FLAG-NS1 WT or mutant constructs using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2021Quote: ... The proteins were expressed in HEK293 Freestyle cells (Invitrogen) in suspension culture using serum-free media (Invitrogen ...
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bioRxiv - Molecular Biology 2020Quote: HeLa cells (ATCC CCL-2) and HEK293-FT (ThermoFisher) were cultured at 37 °C in 5% CO2 in medium composed of high glucose Dulbecco’s modified eagles medium (DMEM ...
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bioRxiv - Microbiology 2022Quote: ... Immunostaining of NS1 and TRIM25 was realized by using the following antibody combination: mouse anti-NS1/goat anti-mouse Alexa 488 (ThermoFisher Scientific) and rabbit anti-TRIM25 (abcam)/goat anti-rabbit Atto647N (sigma) ...
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bioRxiv - Microbiology 2020Quote: HEK293 were transfected with 3xFlag-tagged plasmids encoding each SARS-CoV-2 protein by Lipofectamine 3000 (Thermo Fisher Scientific, #L3000015). 3×107 HEK293 cells were harvested in 10 ml lysis buffer (50 mM Tris-HCl [pH 7.5] ...
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bioRxiv - Microbiology 2023Quote: ... anti-NS1 and anti-NS2 (all 1:2000, Thermo Fisher). Membranes were washed in PBS-T and incubated with fluorescent secondary antibody (anti-mouse or anti-rabbit conjugated to AlexaFluor488 or 647 ...
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bioRxiv - Microbiology 2022Quote: ... pneumoniae serotype 2 strain D39 [17] was plated on brain heart infusion (BHI, Thermo Scientific, MA, USA) agar plates supplemented with 3% v/v defibrinated horse blood (Thermo Scientific ...
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bioRxiv - Microbiology 2021Quote: HEK293/TLR4 and HEK293/null cells (both Invitrogen) were maintained in 4.5 g/L glucose DMEM (Gibco ...
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bioRxiv - Immunology 2021Quote: ... 25 μl of virus was immediately mixed with 25 μl of serially diluted (2×) protein A/G purified IgG (ThermoFisher) from mouse sera (starting at 500 μg/ml ...
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bioRxiv - Neuroscience 2022Quote: LC-MS/MS analyses were conducted using either a QExactive Plus Orbitrap (QE, RNase-digested polysomes) or a Velos Pro Elite Orbitrap (Elite, virus polysomes and HEK293 aggregates) mass spectrometer (Thermo Fisher) coupled online to a nanoAcquity UPLC system (Waters Corporation ...
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bioRxiv - Immunology 2021Quote: ... Dengue viruses were grown in C6/36 cells cultured in RPMI 1640 (Gibco 11875-093), L-Glutamine (Gibco 25030-081) ...
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bioRxiv - Microbiology 2022Quote: ... The levels of RNA corresponding to the N protein-encoding gene of SARS-CoV-2 were measured using the TaqMan Fast Virus 1-step Master Mix (Thermo Scientific). Each 20-μL reaction mixture contained 5.0 μL of 4× TaqMan Fast Virus 1-Step Master Mix ...
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bioRxiv - Bioengineering 2023Quote: ... The levels of RNA corresponding to the N protein-encoding gene of SARS-CoV-2 were measured using the TaqMan Fast Virus 1-step Master Mix (Thermo Scientific). Each 20-μL reaction mixture contained 5.0 μL of 4× TaqMan Fast Virus 1-Step Master Mix ...
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bioRxiv - Microbiology 2021Quote: ... For the preparation of the BGLF2-KO virus, HEK293/EBV(dBGLF2) cells (Konishi et al., 2018) were transfected with BZLF1 expression plasmid using Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... HEK293 T-Rex (referred to as HEK293, cat.no. R71007, Invitrogen) and NIH3T3 cells were grown in DMEM Glutamax® (Thermo Fisher Scientific ...
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Modified N-linked glycosylation status predicts trafficking defective human Piezo1 channel mutationsbioRxiv - Biophysics 2020Quote: ... HEK293S GnT1-/- or HEK293 cells (ThermoFisher Scientific, Cat. No. R78007) using Lipofectamine 3000 transfection reagent (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: HEK293 cells (Invitrogen) were grown in DMEM (1g/L glucose ...
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bioRxiv - Synthetic Biology 2022Quote: HEK293 cells (ThermoFisher; further authenticated by assessing cell morphology and growth rate ...
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bioRxiv - Immunology 2021Quote: ... Virus particles in 2% FBS/DMEM/1mM Pen/Strep (Thermofisher, 15140122) were incubated with cells for 2 hours at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... and the concentration of total virus proteins were determined by a BCA protein assay kit (Thermo Fisher) (31 ...
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bioRxiv - Immunology 2023Quote: ... The recombinant NS1 gene was cloned into the pTRC–His2c expression vector (Invitrogen) and expressed in the BirA-enzyme containing E ...
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bioRxiv - Biochemistry 2022Quote: HEK293 and commercially available Flp-In T-REx HEK293 cells (Invitrogen; R78007) were cultured in DMEM (GIBCO ...
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bioRxiv - Genetics 2023Quote: HEK293 cell protein was extracted with 0.3 mL of RIPA buffer (ThermoFisher, Cat. No. 89900) containing Protease Inhibitor Cocktail (ThermoFisher ...
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bioRxiv - Cell Biology 2020Quote: ... HEK293 cells (ThermoFisher Scientific) were cultured in DMEM/F-12 ...
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bioRxiv - Bioengineering 2022Quote: ... FreeStyle HEK293 cells (ThermoFisher) were used for recombinant S protein production ...
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bioRxiv - Molecular Biology 2019Quote: ... HEK293 FT cells (ThermoFisher) were co-transfected with plasmids pCMV-dR8.2 dvpr (gag-pol ...
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bioRxiv - Synthetic Biology 2019Quote: HEK293 cells (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: HEK293 cells (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293-FT (Invitrogen # R70007) cells were grown in Dulbecco’s Modified Eagle Medium (ThermoFisher #11965118 ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293 (Thermo Fisher Scientific) were cultured in DMEM (Dulbecco’s Modified Eagle Medium ...
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bioRxiv - Immunology 2022Quote: ... or mouse anti-Hepatitis C virus Core protein (clone C7-50, Life Technologies) were visualised using IRDye 800CW Donkey anti-Rabbit IgG or Goat anti-Mouse IgG (LiCor) ...
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bioRxiv - Cell Biology 2020Quote: Live HUVECs and HEK293 cells were stained with Laurdan dye (6-dodecanoyl-2-dimethylaminonaphthalene) (ThermoFisher) at 10 μM (HUVECs ...
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bioRxiv - Immunology 2021Quote: ... 2 × 106 CHO-hCD4 or HEK293-hCD4 were non-enzymatically detached using dissociation buffer (Gibco) and suspended in 5% fetal bovine serum (FBS ...
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bioRxiv - Physiology 2023Quote: Cell surface proteins from transfected HEK293 cells were biotinylated using the Pierce Cell Surface Protein Isolation Kit (ThermoFisher Scientific., cat# 89881). Briefly ...
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bioRxiv - Microbiology 2022Quote: ... The protein concentration of the purified virus preparations was determined using a BCA protein assay kit (Thermo Fisher Scientific) prior to the addition of lysis buffer ...
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bioRxiv - Biochemistry 2022Quote: HEK293 wild type (ATCC, #CRL-1573) and HEK293 Flp-in T-REx (ThermoFisher, #R78007) wild type cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM ...
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bioRxiv - Molecular Biology 2024Quote: HEK293 Flp-In T-REx cells (or HEK293 T-REx; Thermo Fisher Scientific, R78007) and HEK293 cells [American Type Culture Collection (ATCC) ...
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bioRxiv - Cancer Biology 2021Quote: HEK293-FT (Thermo Fisher Scientific) were cultured in DMEM supplemented with 10% FBS ...
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bioRxiv - Bioengineering 2021Quote: ... HEK293 Freestyle suspension cells (ThermoFisher), and immortalized human cord blood-derived mesenchymal stromal cells (cbMSCs ...
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bioRxiv - Neuroscience 2020Quote: HEK293-FT (RRID:CVCL_6911, Thermo Scientific) cells were grown in DMEM containing 10% FBS ...
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bioRxiv - Molecular Biology 2022Quote: HEK293-FlpIn cells (ThermoFisher Scientific) were transfected with empty vector (negative control ...
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bioRxiv - Biochemistry 2022Quote: HEK293 FlpIn-TRex cells (Invitrogen) were cultured in DMEM (Gibco ...