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No products found
because this supplier's products are not listed.
Joanne Durgan, et al.,
bioRxiv - Cell Biology 2020
Quote:
... Cells at 2 × 106 cells/ml in BalanCD medium (Irvine Scientific) were transfected with 1 microgram plasmid/106 cells in 100-ml batches ...
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No products found
because this supplier's products are not listed.
Md. Golam Kibria, et al.,
bioRxiv - Biophysics 2022
Quote:
... 500 μL of protein solution was placed in a 2 mm path-length quartz cuvette (TOSOH, Japan), and spectra were collected in a continuous scanning mode from 260 to 200 nm wavelength ...
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No products found
because this supplier's products are not listed.
Kartika Padhan, et al.,
bioRxiv - Immunology 2020
Quote:
... We sorted 1-2 million cells from each cell types for experiments involving FCS2 chamber (Bioptechs) and 100,000-200,00 cells for experiments involving 8-well chamber (Lab-Tek) ...
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No products found
because this supplier's products are not listed.
James D. Dahlvang, et al.,
bioRxiv - Immunology 2022
Quote:
... PBMCs were counted and resuspended at 2×108 cells/mL in 10mL of PBS + 2% FBS (PEAK Serum, PS-FB1) + 1 mM EDTA (SC Buffer) ...
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No products found
because this supplier's products are not listed.
Han Kang Tee, et al.,
bioRxiv - Microbiology 2019
Quote:
... The viral loads in organs were determined using TaqMan fast virus 1-step master mix (ABI, USA). One step RT-PCR was also performed to amplify viral RNA from the organs using MyTaq One-Step RT-PCR kit (Bioline ...
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No products found
because this supplier's products are not listed.
Janhavi Prasad Natekar, et al.,
bioRxiv - Microbiology 2022
Quote:
Tissues harvested from virus-inoculated animals were weighed and homogenized in a bullet blender (Next Advance, Averill Park, NY, USA) using stainless steel or zirconium beads ...
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No products found
because this supplier's products are not listed.
Ioannis Oikonomakos, et al.,
bioRxiv - Developmental Biology 2023
Quote:
... cells were cultured from day 0 to day 2 in 1:1 ReproFF2 (REPROCELL RCHEMD006) or StemFit™ Basic04CT (REPROCELL ASB04CT) ...
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Lenti/Retro Virus
Cat# LVB1000,
Conservation Buffer (1mL), USD $95.00/mL
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Olumayokun A Olajide, et al.,
bioRxiv - Neuroscience 2021
Quote:
... Transfection of BV-2 cells was performed by preparing a Glia-Mag transfection reagent (OZ Biosciences) and Cignal NF-κB luciferase reporter (Qiagen ...
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No products found
because this supplier's products are not listed.
Leah M. Williams, et al.,
bioRxiv - Cell Biology 2019
Quote:
... a piece of tissue approximately 2 cm by 2 cm was placed into a glass dounce tissue grinder (Wheaton USA) with 1 ml of AT Lysis Buffer and protease inhibitors (described above) ...
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No products found
because this supplier's products are not listed.
Immacolata Porreca, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... T cells were lysed using DirectPCR (cell) (Viagen Biotech, LA, USA) lysis buffer supplemented with proteinase K (10 µg/mL ...
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No products found
because this supplier's products are not listed.
Teri N. Hreha, et al.,
bioRxiv - Immunology 2023
Quote:
... O and K serotypes (1:200, Meridian Life Science #B65109G), Rat anti-Ly6G-APC (1:200 ...
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Recombinant AAV-2 VP1 Protein was expressed in E. coli.
Cat# VP1-1787A,
10ug , USD $298
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Yan Qi, et al.,
bioRxiv - Molecular Biology 2024
Quote:
... the presence of anti- Ad and anti-hemagglutinin antibodies was measured by ELISA against a purified Ad6 virus preparation (Greffex, Inc.) and a recombinant hemagglutinin protein (Creative Biomart). In the first case ...
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No products found
because this supplier's products are not listed.
Angela Ma, et al.,
bioRxiv - Microbiology 2022
Quote:
... DFA kits were used to test for herpes simplex virus types 1 and 2 (D3 DFA HSV Identification and Typing kit, Quidel, San Diego, CA), varicella-zoster virus (Light Diagnostics ...
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No products found
because this supplier's products are not listed.
Shiwei Liu, et al.,
bioRxiv - Genomics 2023
Quote:
... we grew parasites at 37°C in vitro at 3% hematocrit (serotype A positive human erythrocytes, Valley Biomedical, VA or BioIVT, NY) in RPMI 1640 medium (Invitrogen ...
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No products found
because this supplier's products are not listed.
Michihito Sasaki, et al.,
bioRxiv - Microbiology 2020
Quote:
... the original stock of this virus (wild type, WT) was prepared by inoculation of Vero-TMPRSS2 cells with Mynox mycoplasma elimination reagent (Minerva Biolabs) [11] ...
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No products found
because this supplier's products are not listed.
Aleksei Kuznetsov, et al.,
bioRxiv - Biochemistry 2020
Quote:
... and SARS-CoV-2 Spike protein S1 (Icosagen OÜ, Estonia, cat# P-305-100) were used in this study.
-
No products found
because this supplier's products are not listed.
Chengjin Ye, et al.,
bioRxiv - Microbiology 2022
Quote:
... and SARS-CoV-2 M protein was purchased from ProteoGenix (Catalog# PX-COV-P025).
-
No products found
because this supplier's products are not listed.
Hsuan-Hsuan Lu, et al.,
bioRxiv - Cancer Biology 2019
Quote:
... cells were pre-treated with Afatinib (2 μM; T2303, TargetMol) or LY2157299 (10 μM ...
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No products found
because this supplier's products are not listed.
Marwa K. Ibrahim, et al.,
bioRxiv - Microbiology 2021
Quote:
Cells were lysed with PRO-PREP protein extraction solution (Intron Biotechnology). Protein samples were separated on a 12% gel via SDS-PAGE ...
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No products found
because this supplier's products are not listed.
Dorit Möhrle, et al.,
bioRxiv - Animal Behavior and Cognition 2023
Quote:
... 2) KD (weight ratio of fat to carbohydrate plus protein is approximately 6.3:1, F3666, Bio-serv, NJ). Percentage calories provided by each nutrient category are ...
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No products found
because this supplier's products are not listed.
Gila Lustig, et al.,
bioRxiv - Microbiology 2019
Quote:
... Supernatant containing released virus was harvested two days post-transfection and filtered through a 0.45 micron filter(GVS) and stored in 0.5ml aliqouts at −80°C ...
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No products found
because this supplier's products are not listed.
Vitor Mendes, et al.,
bioRxiv - Biochemistry 2020
Quote:
... was mixed in 1:1 and 1:2 (protein to reservoir) ratio with well solution using a mosquito robot (TTP labtech). Initial conditions were obtained in the Classics lite crystallization screen (Qiagen) ...
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No products found
because this supplier's products are not listed.
Carly Strelez, et al.,
bioRxiv - Cancer Biology 2023
Quote:
Human umbilical vein endothelial cells (HUVEC) expressing Red Fluorescent Protein (RFP) (Angio-Proteomie, #cAP-0001RFP) were expanded in EBM-2 media with EGM-2 SingleQuots Supplements (2% FBS ...
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No products found
because this supplier's products are not listed.
Abbigayl E.C. Burtis, et al.,
bioRxiv - Immunology 2024
Quote:
... Cells were washed 2x with PBS containing 2% FBS (Atlas Biologicals, Fort Collins, CO). Viable T cells were enriched using the Easy Sep T cell isolation Kit followed by the Easy Sep Dead Cell removal Kit (Stem Cell Technologies ...
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No products found
because this supplier's products are not listed.
Svetlana Glushakova, et al.,
bioRxiv - Biophysics 2022
Quote:
... The mechanical activation of PIEZO1 in RBC by shear stress was achieved by constant agitation of cells (1-2 × 108 cells/ml) on the platform of a “Belly Button Shaker” (Model BBUAAUV15, Ibi Scientific) at a speed of 14 rpm ...
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No products found
because this supplier's products are not listed.
Bettina P Mihalas, et al.,
bioRxiv - Cell Biology 2023
Quote:
... Cells were incubated in human polyclonal anti-centromere protein antibody (1:50; Antibodies Incorporated, 15-235), rabbit polyclonal anti-SGOL2 antibody (1:200 ...
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No products found
because this supplier's products are not listed.
Mads Kuhlmann Andersen, et al.,
bioRxiv - Physiology 2022
Quote:
... 5 μL of crude homogenate and protein standards (0 to 2 mg mL-1 bovine serum albumin, ALB001.25, Bioshop Canada, Burlington, ON, CA) was loaded into wells in triplicate followed by 250 μL of Bradford reagent (B6916 ...
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No products found
because this supplier's products are not listed.
Brenton T. Laing, et al.,
bioRxiv - Neuroscience 2022
Quote:
Immunostaining was conducted for axonal projections and verification of virus expression using a chicken anti-GFP polyclonal antibody (1:1000, Cat # GFP-1020, RRID:AB_10000240; Aves Labs, CA, U.S.A.) or a rabbit anti-DsRed polyclonal antibody (1:1000 ...
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No products found
because this supplier's products are not listed.
Dora Buzas, et al.,
bioRxiv - Biochemistry 2023
Quote:
... Control wells containing virus only (no ADAH11) as well as a positive control (a commercial monoclonal antibody (Absolute Antibody; Sb#15) recognising the S protein RBD ...
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No products found
because this supplier's products are not listed.
Chengyao Wang, et al.,
bioRxiv - Bioengineering 2020
Quote:
Human intestinal epithelial cells Caco-2 (ATCC® HTB-37™) were cultured in a 75 cm2 cell culture flask (Nest Biotechnology) with complete 1X Eagle’s Modified Eagle Medium (EMEM ...
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No products found
because this supplier's products are not listed.
Gabriella A. Bertaccini, et al.,
bioRxiv - Biophysics 2023
Quote:
... differentiated cells were passaged with 2 mg/mL of Dispase (Cat. No. CnT-DNP-10, Cellntec) incubated for 30 min at 37C ...
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No products found
because this supplier's products are not listed.
Mostafa Jarahian, et al.,
bioRxiv - Immunology 2020
Quote:
... Complexes of NCR-Fc fusion proteins (1 to 2 μg per staining) and PE-labeled goat anti-hIgG Fc (Dianova; 1:100 in FACS buffer) were allowed to form for 30 min before being added to cells for 60 min on ice ...
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No products found
because this supplier's products are not listed.
Jessica T. Stieglitz, James A. Van Deventer,
bioRxiv - Synthetic Biology 2021
Quote:
... (S)-2-amino-6-((2-azidoethoxy)carbonylamino)hexanoic acid (Iris Biotech), (S)-2-amino-6-(((prop-2-yn-1-yloxy)carbonyl)amino)hexanoic acid (AstaTech) ...
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No products found
because this supplier's products are not listed.
Kristin D. Dahl, et al.,
bioRxiv - Developmental Biology 2022
Quote:
... MBP (Myelin Basic Protein, Neuromics Cat # ...
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No products found
because this supplier's products are not listed.
R. Wercberger, et al.,
bioRxiv - Neuroscience 2020
Quote:
... 2% Fluoro-Gold (Fluorochrome), or the HSV-hEF1a-GFP-L10a.
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No products found
because this supplier's products are not listed.
Celia Fernandez-Sanz, et al.,
bioRxiv - Physiology 2021
Quote:
Equal amounts of protein (70 μg) supplemented with 5x Protein Loading Buffer (National Diagnostics, USA) were preheated (95°C ...
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No products found
because this supplier's products are not listed.
Yi Sak Kim, et al.,
bioRxiv - Neuroscience 2024
Quote:
BV-2 microglia cell line was cultured in DMEM with 5% FBS and 50 μg/ml gentamicin (Omega Scientific) as described previously5 ...
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No products found
because this supplier's products are not listed.
O Andrini, et al.,
bioRxiv - Physiology 2024
Quote:
... Worms were imaged on 2% dry agar pads mounted in M9 solution containing 2% microbeads (Tebu-Bio, polystyrene polybeads 0.10 µm microsphere) ...
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No products found
because this supplier's products are not listed.
Yasunori Watanabe, et al.,
bioRxiv - Microbiology 2020
Quote:
... Protein was then stained using methylamine tungstate (Nanoprobes) before being allowed to dry at room temperature for 15 minutes ...
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No products found
because this supplier's products are not listed.
Chris Y. Cheung, et al.,
bioRxiv - Cell Biology 2021
Quote:
... His-KPNA5 protein was obtained from LifeSpan BioSciences, Inc ...
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No products found
because this supplier's products are not listed.
Yeranuhi Hovhannisyan, et al.,
bioRxiv - Pathology 2023
Quote:
... and CW30318 (Control 2, Fuji Cellular Dynamics) derived from healthy individuals without any cardiac pathology ...
-
No products found
because this supplier's products are not listed.
Lindsay Smith, et al.,
bioRxiv - Developmental Biology 2020
Quote:
One-cell stage zebrafish embryos were injected with 10 pg of a cDNA construct containing a fluorescent protein attached to a PIP-binding protein domain [Bodipy-PI (Echelon Biosciences Inc)] ...
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No products found
because this supplier's products are not listed.
Daniel Wrapp, et al.,
bioRxiv - Microbiology 2021
Quote:
... 3 μL of protein was deposited on an UltrAuFoil 1.2/1.3 grid that was plasma cleaned at 25 mA for 2 minutes using a Solarus plasma cleaner (Gatan). Excess liquid was blotted away for 3 seconds in a Vitrobot Mark IV (FEI ...
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No products found
because this supplier's products are not listed.
Ugo Sardo, et al.,
bioRxiv - Physiology 2023
Quote:
Liver proteins were extracted by physical dissociation (Ultra-turrax, IKA) in PEB Buffer (150 mM NaCl ...
-
No products found
because this supplier's products are not listed.
Wilton T. Snead, et al.,
bioRxiv - Biophysics 2021
Quote:
... Protein solutions were loaded into glass-bottom imaging chambers (Grace Bio-Labs). To prevent protein adsorption to the imaging surface ...
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No products found
because this supplier's products are not listed.
Eamim Daidrê Squizani, et al.,
bioRxiv - Microbiology 2020
Quote:
... and homogenized in 2 ml sterile PBS using a BeadBug (Benchmark Scientific). Organ homogenates were serially diluted and plated onto Sabouraud dextrose agar plates ...
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No products found
because this supplier's products are not listed.
Olha Zapukhliak, et al.,
bioRxiv - Neuroscience 2020
Quote:
... amplified using a 2-channel differential amplifier M1800 (A-M Systems, Carlsborg, WA), digitized at 10 kHz using an analog-to-digital converter (NI PCI-6221 ...
-
No products found
because this supplier's products are not listed.
Guodong Wang, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Cells were harvested by cell lifter (Biologix, 70-2180) 72-h after co-transfection with PHP.eB (Addgene ...
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No products found
because this supplier's products are not listed.
Federico Tessadori, et al.,
bioRxiv - Developmental Biology 2020
Quote:
Cell counting: cell counting was carried out in Volocity (Quorum technologies) on confocal-acquired 3D stacks.All statistical assays were carried out in Graphpad Prism 6.0 (GraphPad Software).
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No products found
because this supplier's products are not listed.
M. Chighizola, et al.,
bioRxiv - Biophysics 2020
Quote:
... and plated in low concentration of 4.000 cells/cm2 (to guarantee the presence of single separated cells) on Ø 40 mm glass-bottom dishes for cell culture (Willco Wells) the day before the experiment ...