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No products found
because this supplier's products are not listed.
Ennio d’Amico, et al.,
bioRxiv - Biochemistry 2022
Quote:
The following Dynactin coding genes were codon-optimized for protein expression in insect cells and synthesized (Epoch Life Science): DCTN1 (Protein name p150 ...
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No products found
because this supplier's products are not listed.
Aniruddha R. Agnihotri, et al.,
bioRxiv - Molecular Biology 2019
Quote:
Spodoptera frugiperda (Sf9) insect cell line was purchased from the National Centre from Cell Sciences (NCCS) cell repository and was maintained in Grace’s insect medium (HiMedia, ...
-
No products found
because this supplier's products are not listed.
Viviane Kremling, et al.,
bioRxiv - Molecular Biology 2022
Quote:
Constructs were amplified from insect cell codon-optimized DNA fragments (Bio Basic Inc., Canada) for protein production in High Five insect cells ...
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No products found
because this supplier's products are not listed.
Jason Neidleman, et al.,
bioRxiv - Immunology 2020
Quote:
... or Influenza Virus Control Peptide Pool (Anaspec). As a positive control for cytokine detection ...
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No products found
because this supplier's products are not listed.
Zihao Wang, et al.,
bioRxiv - Molecular Biology 2024
Quote:
... pH 7.0 (The Protein Complex Suite F1, Molecular Dimensions, protein: precipitant ratio 2:1). The crystal was harvested and flash-cooled without adding cryo-protectant ...
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CSC 2F0 cells were isolated by centrifugal counterflow elutriation from 250 individual donor...
Cat# CSC 2F0 V,
1.0 Units, $405.0
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Lorna Ewart, et al.,
bioRxiv - Pharmacology and Toxicology 2022
Quote:
... 2% Culture-Boost (Cell Systems), and 10% Fetal Bovine Serum (FBS ...
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No products found
because this supplier's products are not listed.
Bernarda Calla, et al.,
bioRxiv - Genomics 2019
Quote:
... DNA was extracted using an E.Z.N.A.® insect DNA kit (Omega Bio-tek, Norcross, GA) according to the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Jakob Farnung, et al.,
bioRxiv - Biochemistry 2022
Quote:
... Protein solution (2 μL) was mixed with reservoir solution (2 μL) on siliconized cover-slips (Hampton Research). Crystallizations were performed at room temperature ...
-
No products found
because this supplier's products are not listed.
Daniel Wells, et al.,
bioRxiv - Genetics 2019
Quote:
... The purified protein was used to immunize 2 rabbits (Eurogentec, Belgium), and the resulting immune antisera were tested against the recombinant antigen by ELISA (Eurogentec) ...
-
No products found
because this supplier's products are not listed.
James Brett Case, et al.,
bioRxiv - Microbiology 2020
Quote:
... Virus was plaque-purified on Vero CCL81 cells in the presence of 25 μg/ml of cytosine arabinoside (TriLink BioTechnologies), and plaques in agarose plugs were amplified on Vero CCL81 cells ...
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No products found
because this supplier's products are not listed.
Laura Schenkel, et al.,
bioRxiv - Cell Biology 2023
Quote:
... The cells were fixed in 2 % paraformaldehyde (Polyscience; Hirschberg an der Bergstrasse ...
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No products found
because this supplier's products are not listed.
Paul K. LaFosse, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Virus was injected unilaterally with a stereotactic syringe pump (Stoelting; Wood Dale, IL) through a pulled glass pipette tip cut to an opening of 10–15 μm diameter ...
-
No products found
because this supplier's products are not listed.
Noémie Aurine, et al.,
bioRxiv - Cell Biology 2019
Quote:
... or universal IFN-type (a recombinant human IFN-alpha protein hybrid; Pbl Assay Science, 11200-2). Bat cells seeded in 12-well plates at 80% confluence were treated for 6 h at 37°C with either poly IC (0–1000 ng/mL ...
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No products found
because this supplier's products are not listed.
Yuma Nishikawa, et al.,
bioRxiv - Synthetic Biology 2024
Quote:
... Protein was extracted from the cells using E.coli/Yeast Protein Extraction Buffer (Tokyo Chemical Industry Co., Ltd., Tokyo, Japan), and the lysate fraction was used for analysis.
-
WB,ELISA
Cat# A5244, SKU# A5244-100ul,
100ul, $157.00
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Qiong Wang, et al.,
bioRxiv - Microbiology 2022
Quote:
... Virus quantification by real-time PCR was performed using the QPCR Mix (Bimake, Cat#B21202), following the supplier’s instruction ...
-
No products found
because this supplier's products are not listed.
Sophie E. Cousineau, et al.,
bioRxiv - Microbiology 2022
Quote:
... the media on these cells was changed for complete Huh-7.5 media with 5 μM 2’CMA (2’C-methyladenosine, Carbosynth), an HCV NS5B polymerase inhibitor ...
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No products found
because this supplier's products are not listed.
Sara Y. Brucker, et al.,
bioRxiv - Developmental Biology 2021
Quote:
... and the desired amount of cell suspension mixed with Basement Membrane Extract (Type 2, 3533-001-2, Trevigen; BME) at a ratio of 65% BME to 35% cell suspension ...
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No products found
because this supplier's products are not listed.
Gerda E. Breitwieser, et al.,
bioRxiv - Neuroscience 2023
Quote:
... phospho- and total ERK1/2 were determined using the two-plate adherent cell protocol (Phosph-ERK1/2 kit, Cisbio), following manufacturer’s instructions ...
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No products found
because this supplier's products are not listed.
Luisa Henkel, et al.,
bioRxiv - Genomics 2020
Quote:
... successfully transduced cells were selected with 2 µg/ml puromycin (Biomol). One plate was cultured in the absence of puromycin for MOI determination ...
-
No products found
because this supplier's products are not listed.
Christopher Rovera, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... cells were treated with 2 ng/ml TGF-β1 (#11343160, ImmunoTools), 2 ng/ml LIF (#300-05 ...
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No products found
because this supplier's products are not listed.
Srinivasan Sivanandan, et al.,
bioRxiv - Genomics 2023
Quote:
... Next the samples were transformed into electrocompetent cells (Biosearch Technologies, 60242-2). One 0.1cm cuvette (Bio-Rad ...
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No products found
because this supplier's products are not listed.
H. Tai Nguyen, et al.,
bioRxiv - Cancer Biology 2022
Quote:
Western blot analyses of FANC protein expression were performed using total cell protein extracts (∼40 μg from ∼1-2.5 × 106 cells) prepared in RIPA Lysis Buffer (Boster Biological Technology ...
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No products found
because this supplier's products are not listed.
Yoshiaki Nishimura, et al.,
bioRxiv - Microbiology 2020
Quote:
... Cells were grown to ~90% confluency in a 96-well plate prior to addition of serially diluted virus to achieve luminescence signals of 30,000-1,000,000 (BMG Labtech PolarStar Optima plate reader at maximum gain) ...
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No products found
because this supplier's products are not listed.
Matthew H. Van Dam, et al.,
bioRxiv - Genomics 2020
Quote:
... sequencing one cell with CLR version 2 chemistry (PacBio, Menlo Park, CA, USA).
-
No products found
because this supplier's products are not listed.
Jin-Young Yoon, et al.,
bioRxiv - Pathology 2022
Quote:
... These cells were loaded with Rhod-2 AM (AAT Bioquest, Inc, Catalog #21062) at room temperature for 30 minutes ...
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No products found
because this supplier's products are not listed.
Maria Font Farre, et al.,
bioRxiv - Plant Biology 2023
Quote:
... 44.5 μL of labelled proteins were incubated with 2 μM Cy5 Picolyl Azide (Click Chemistry Tools, 50 μM stock in DMSO) or Fluorescein Picolyl Azide (Click Chemistry Tools ...
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No products found
because this supplier's products are not listed.
Jennine M. Dawicki-McKenna, et al.,
bioRxiv - Molecular Biology 2022
Quote:
Total protein extraction from cells was performed using 1.5x Laemmli buffer [15% glycerol (Amresco #M152), 3% SDS (Sigma #L5750) ...
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No products found
because this supplier's products are not listed.
Simon P. Fraessle, et al.,
bioRxiv - Immunology 2022
Quote:
... 2×104 Target cells were seeded into 96 well E-Plate (ACEA Biosciences Inc.) and rested overnight ...
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No products found
because this supplier's products are not listed.
Jooman Park, et al.,
bioRxiv - Physiology 2023
Quote:
... total proteins from tissue and SVF cells were lysed with RIPA buffer (Boston BioProducts, Boston, MA) supplemented with protease inhibitor cocktail (MedChem Express ...
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No products found
because this supplier's products are not listed.
April H. Nguyen, et al.,
bioRxiv - Microbiology 2023
Quote:
Protein-protein interactions were evaluated using the Bacterial Two Hybrid System (Euromedex) according to the manufacturer instructions ...
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No products found
because this supplier's products are not listed.
Gaurav Mendiratta, et al.,
bioRxiv - Cancer Biology 2021
Quote:
SK-MEL-2 cells were grown in EMEM (HyClone) supplemented with 10% fetal bovine serum (Atlanta Biologicals) and Penicillin-Streptomycin (Gibco) ...
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No products found
because this supplier's products are not listed.
Logan R. Hurst, et al.,
bioRxiv - Cell Biology 2020
Quote:
... 2% dextrose (RPI)] ...
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No products found
because this supplier's products are not listed.
Brianna M. Doratt, et al.,
bioRxiv - Immunology 2023
Quote:
Freshly thawed UCBMCs (1-2×106 cells) were stained with Ghost Violet 540 (Tonbo Biosciences, San Diego, CA) for 30 min at 4C in the dark before being incubated with Fc blocker (Human TruStain FcX ...
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No products found
because this supplier's products are not listed.
Laura Maria Florez, et al.,
bioRxiv - Immunology 2023
Quote:
... The muMECs were seeded in T75 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min (from Cell Biologics, Euromedex) and cultures were divided twice a week or as necessary ...
-
No products found
because this supplier's products are not listed.
Joydeb Sinha, et al.,
bioRxiv - Cell Biology 2023
Quote:
... Harvested virus was 0.4μM filtered (Celltreat 229749) and concentrated by centrifugation using a 100kdA cut-off PES protein concentrator (Pierce 88533 ...
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No products found
because this supplier's products are not listed.
Shiyu Sun, et al.,
bioRxiv - Immunology 2021
Quote:
... The coding sequence for SARS-CoV-2 RBD spanning S protein 319-541 (GenBank: YP_009724390) was codon-optimized for mammalian cells and synthesized by GENEWIZ, China ...
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No products found
because this supplier's products are not listed.
Brogan Ashley, et al.,
bioRxiv - Physiology 2021
Quote:
... or 2 μM Receptor Associated Protein (RAP; Innovative Research, USA), which block pinocytosis ...
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No products found
because this supplier's products are not listed.
Hunter K. Walt, et al.,
bioRxiv - Molecular Biology 2024
Quote:
... insects were individually placed into tubes of RNAzol RT reagent (Molecular Research Center, Cincinnati, OH) and frozen at −80°C for total RNA isolation ...
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No products found
because this supplier's products are not listed.
Sara M. Eslami, et al.,
bioRxiv - Bioengineering 2023
Quote:
... Quartz spectrophotometer cell (Starna Cells, cat. no. 1-Q-2) and measured using an Olis Cary-16 circular dichroism spectrometer ...
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No products found
because this supplier's products are not listed.
Justin J. Botterill, et al.,
bioRxiv - Neuroscience 2021
Quote:
Virus was delivered using a 500nL Neuros Syringe (#65457-02, Hamilton Company) attached to the stereotaxic apparatus with a probe holder (#751873 ...
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No products found
because this supplier's products are not listed.
Nurit P. Azouz, et al.,
bioRxiv - Immunology 2023
Quote:
... glycerol) with 250 ng total protein from cell extracts or 120 ng recombinant GST-tagged OVOL1 protein (Abnova #H00005017-P01) and non-fluorescent competitor containing the wild-type or mutant binding site for 30 minutes at room temperature and cross-linked at 120 mJ/cm2 ...
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No products found
because this supplier's products are not listed.
Michael D. Vahey, Daniel A. Fletcher,
bioRxiv - Microbiology 2019
Quote:
... replacing media with virus growth media supplemented with or without a specified concentration of oseltamivir carboxylate (Toronto Research Chemicals O700980), but without TPCK-treated trypsin ...
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No products found
because this supplier's products are not listed.
Melissa H. Bergeman, et al.,
bioRxiv - Microbiology 2023
Quote:
... Image segmentation and 3D rendering of HSV-1 virus particle undergoing exocytosis from Rab6a vesicle was done in Imaris (Oxford Instruments) by constructing spots and surfaces for the objects at respective image slices ...
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No products found
because this supplier's products are not listed.
Ourania Galanopoulou, et al.,
bioRxiv - Cell Biology 2023
Quote:
... Protein A/G-fused Tn5 transposase protein (CUTANA pA/G-Tn5, Epicypher) was added and the samples were incubated for 1 hour at room temperature followed by washing with a buffer containing 20 mM HEPES ...
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No products found
because this supplier's products are not listed.
Belen Esquerra-Ruvira, et al.,
bioRxiv - Molecular Biology 2022
Quote:
NZYBlue Protein Marker (NZYtech) was used for protein size estimation and protein concentrations were measured with QUBIT® 2.0 (Invitrogen).
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No products found
because this supplier's products are not listed.
Kalpana Mandal, et al.,
bioRxiv - Biophysics 2019
Quote:
... cells were incubated overnight with 2-μm-diameter fluorescently labeled latex beads (660/690 fluorescence; Bangs Laboratories) before seeding them on the micropatterns ...
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No products found
because this supplier's products are not listed.
Jessica Cheng, et al.,
bioRxiv - Genetics 2022
Quote:
... Cell nuclei were resuspended in 2 mL 0.4% SDS shearing buffer for sonication with a Covaris S220X sonicator (Covaris) for 7 min ...
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No products found
because this supplier's products are not listed.
Hannah J. MacMillan, et al.,
bioRxiv - Genomics 2022
Quote:
Total RNA was isolated from EndoC cells using the Norgen RNA/DNA/Protein Purification Kit (Norgen Biotek Corp. Thorold, Ontario, Canada) and subjected to reverse transcription using the SuperScript IV VILO MasterMix kit (Thermo Fisher Scientific ...
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No products found
because this supplier's products are not listed.
Timothy S. Balmer, Laurence O. Trussell,
bioRxiv - Neuroscience 2019
Quote:
... fluorescent microspheres (Spherotech, FP4060-2) were imaged following the same procedures used for biocytin filled cells (Figure S5).
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No products found
because this supplier's products are not listed.
Iain C. Clark, et al.,
bioRxiv - Genomics 2022
Quote:
... 280 μL of oil (Fluent Biosciences # FB0001804) was added to the cell-bead mixture and vortexed (Vortex Genie 2, Scientific Industries) using a custom adapter (Fluent BioSciences ...